LOCUS       Exported               10159 bp ds-DNA     circular SYN 27-APR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10159)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 7, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10159
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        10..385
                     /label=TRE3G promoter
     primer_bind     368..411
                     /label=lentiTO-d37-ppm1h-F
     primer_bind     368..408
                     /label=Tre3g-frag1-PPM1H-Fw
     misc_feature    395..1830
                     /label=PPM1H
     misc_feature    1838..2524
                     /label=TMEM115 1-229
     primer_bind     complement(2504..2545)
                     /label=lentiTO_tmem_R
     misc_feature    2531..2557
                     /label=HA-tag
                     /note="HA-tag"
     misc_feature    2567..3112
                     /label=EF1 promoter
     misc_feature    3112..3113
                     /label=GCTAGAC
                     /note="Geneious type: Editing History Deletion"
     gene            3119..3862
                     /label=rtta3G
     CDS             3872..3928
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     gene            3929..4327
                     /label=Blasticidin Resistance
     misc_feature    3929
                     /label=ATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTT...
                     /note="Geneious type: Editing History Deletion"
     misc_feature    4334..4923
                     /label=WPRE
     misc_feature    4697..4698
                     /label=G
                     /note="Geneious type: Editing History Deletion"
     misc_feature    complement(6774..7634)
                     /label=AmpR (beta-lactamase)
                     /note="AmpR (beta-lactamase)"
     misc_feature    9317..9549
                     /label=RRE
     misc_feature    9550..10038
                     /label=Env
     misc_feature    9550..10038
                     /label=Env
ORIGIN
        1 gatactagtt ttactcccta tcagtgatag agaacgtatg aagagtttac tccctatcag
       61 tgatagagaa cgtatgcaga ctttactccc tatcagtgat agagaacgta taaggagttt
      121 actccctatc agtgatagag aacgtatgac cagtttactc cctatcagtg atagagaacg
      181 tatctacagt ttactcccta tcagtgatag agaacgtata tccagtttac tccctatcag
      241 tgatagagaa cgtataagct ttaggcgtgt acggtgggcg cctataaaag cagagctcgt
      301 ttagtgaacc gtcagatcgc ctggagcaat tccacaacac ttttgtctta taccaacttt
      361 ccgtaccact tcctaccctc gtaaatctag aatgcgtttc ccctacgggc ggccagagtt
      421 cctggggctg tctcaggacg aggtggagtg cagcgccgac cacatcgccc gccccatcct
      481 catcctcaag gagactcggc ggctgccctg ggccactggc tacgcagagg ttatcaatgc
      541 cgggaagagc acacacaatg aagaccaagc cagctgtgag gtgctcactg tgaagaagaa
      601 ggcaggggcc gtgacctcaa ccccaaacag gaactcatcc aagagacggt cctcccttcc
      661 caatggggaa gggctgcagc tgaaggagaa ctcggaatcc gagggtgttt cctgccacta
      721 ttggtcgctg tttgacgggc acgcggggtc cggggccgcg gtggtggcgt cacgcctgct
      781 gcagcaccac atcacggagc agctgcagga catcgtggac atcctgaaga actccgccgt
      841 cctgccccct acctgcctgg gggaggagcc tgagaacacg cccgccaaca gccggactct
      901 gacccgggca gcctccctgc gcggaggggt gggggccccg ggctccccca gcacgccccc
      961 cacacgcttc tttaccgaga agaagattcc ccatgagtgc ctggtcatcg gagcgcttga
     1021 aagtgcattc aaggaaatgg acctacagat agaacgagag aggagttcat ataatatatc
     1081 tggtggctgc acggccctca ttgtgatttg ccttttgggg aagctgtatg ttgcaaatgc
     1141 tggggatagc agggccataa tcatcagaaa tggagaaatt atccccatgt cttcagaatt
     1201 tacccccgag acggagcgcc agcgacttca gtacctggca ttcatgcagc ctcacttgct
     1261 gggaaatgag ttcacacatt tggagtttcc aaggagagta cagagaaagg agcttggaaa
     1321 gaagatgctc tacagggact ttaatatgac aggctgggca tacaaaacca ttgaggatga
     1381 ggacttgaag ttccccctta tatatggaga aggcaagaag gcccgggtaa tggcaactat
     1441 tggagtgacc aggggacttg gggaccatga cctgaaggtg catgactcca acatctacat
     1501 taaaccattc ctgtcttcag ctccagaggt aagaatctac gatctttcaa aatatgatca
     1561 tggatcagat gatgtgctga tcttggccac tgatggactc tgggacgttt tatcaaatga
     1621 agaagtagca gaagcaatca ctcagtttct tcctaactgt gatccagatg atcctcacag
     1681 gtacacactg gcagctcagg acctggtgat gcgtgcccgg ggtgtgctga aggacagagg
     1741 atggcggata tctaatgacc gactgggctc aggagacgac atttctgtat atgtcattcc
     1801 tttaatacat ggaaacaagc tgtcatcagc ggccgccatg caacgtgccc tgccaggcgc
     1861 ccgccagcac ttgggggcca ttctggccag cgccagcgtg gtggtgaagg ctctgtgtgc
     1921 ggcggtacta ttcctctacc tgctctcctt cgccgtggac acaggctgcc tggcggtcac
     1981 cccgggctac ctctttcctc ccaacttctg gatctggacc ctggccaccc atgggctgat
     2041 ggagcagcat gtgtgggacg tggccatcag cctgacaacg gtggtggtgg ccgggcgttt
     2101 gctggagccc ctctgggggg ccttggagct gctcatcttc ttctcagtgg tgaatgtgtc
     2161 tgtagggctg ctgggggcct tcgcctacct cctcacctac atggcttcct tcaacctggt
     2221 ctacctgttc actgtccgta tccacggcgc cttgggcttc ctaggtggcg tcctggtggc
     2281 actcaagcaa accatggggg actgtgtggt cctgcgagtg ccccaggtgc gcgtcagtgt
     2341 gatgcccatg ctgctgctgg cgctgctgct cctgctgcgg ctcgccacgc tgctccagag
     2401 cccggcgctg gcttcctatg gcttcgggct gctctccagt tgggtatatc ttcgcttcta
     2461 ccagcgccat agccggggcc gaggggacat ggctgaccac tttgctttcg ccactttctt
     2521 ccctgctagc tacccttacg acgtgccaga ctacgcctaa ggccgcaagg atctgcgatc
     2581 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
     2641 gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
     2701 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
     2761 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc
     2821 gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc catccacgcc
     2881 ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct
     2941 aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct tggagcctac
     3001 ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc tacgtctttg
     3061 tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct acgccaccat
     3121 gagccggctg gacaagagca aagtgatcaa cagcgccctg gaactgctga acggcgtggg
     3181 catcgagggc ctgaccaccc ggaagctggc ccagaaactg ggcgtggaac agcccaccct
     3241 gtactggcac gtgaagaaca agcgggccct gctggacgcc ctgcccatcg agatgctgga
     3301 ccggcaccac acccacagct gccctctgga aggcgagagc tggcaggact tcctgcggaa
     3361 caacgccaag agctacagat gcgccctgct gagccaccgg gacggcgcca aagtgcacct
     3421 gggcaccaga cccaccgaga agcagtacga gacactggaa aaccagctgg ccttcctgtg
     3481 ccagcagggc ttcagcctgg aaaacgccct gtacgccctg agcgccgtgg gccacttcac
     3541 cctgggctgc gtgctggaag aacaggaaca ccaggtcgcc aaagaggaaa gagagacacc
     3601 caccaccgac agcatgcccc ccctgctgaa gcaggccatc gagctgttcg acagacaggg
     3661 cgccgagccc gccttcctgt tcggcctgga actgatcatc tgcggcctcg agaagcagct
     3721 gaagtgcgag agcggcggac ccaccgacgc cctggacgac ttcgacctgg acatgctgcc
     3781 cgccgatgcc ctggatgatt ttgatctgga tatgctccct gctgacgctc tcgatgactt
     3841 cgatctcgat atgctgccag gcggaagcgg agctactaac ttcagcctgc tgaagcaggc
     3901 tggagacgtg gaggagaacc ctggacctat ggccaagcct ttgtctcaag aagaatccac
     3961 cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt
     4021 cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt
     4081 tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg
     4141 caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg
     4201 acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag
     4261 tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga
     4321 gggctaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     4381 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     4441 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     4501 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     4561 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     4621 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     4681 aggggctcgg ctgttggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     4741 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     4801 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     4861 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     4921 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     4981 agaaaagggg ggactggaag ggctaattca ctcccaacga aaataagatc tgctttttgc
     5041 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     5101 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     5161 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     5221 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     5281 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     5341 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     5401 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     5461 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     5521 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac
     5581 ttttgcagag acggcccaaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     5641 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     5701 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     5761 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     5821 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     5881 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     5941 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     6001 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     6061 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     6121 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     6181 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     6241 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     6301 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     6361 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     6421 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     6481 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     6541 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     6601 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     6661 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     6721 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     6781 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     6841 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     6901 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     6961 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     7021 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     7081 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     7141 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     7201 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     7261 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     7321 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     7381 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     7441 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     7501 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     7561 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     7621 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     7681 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     7741 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     7801 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     7861 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     7921 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     7981 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     8041 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt
     8101 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     8161 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     8221 acggccagtg ccaagctgac gcgtgtagtc ttatgcaata ctcttgtagt cttgcaacat
     8281 ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt
     8341 ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg
     8401 gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg
     8461 atacaataaa cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     8521 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     8581 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     8641 aaatctctag cagtggcgcc cgaacaggga cctgaaagcg aaagggaaac cagaggagct
     8701 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     8761 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
     8821 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
     8881 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
     8941 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
     9001 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
     9061 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
     9121 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccactg atcttcagac
     9181 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     9241 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     9301 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     9361 tgggcgcagc ctcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     9421 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     9481 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     9541 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     9601 ggaatgctag ttggagtaat aaatctctgg aacagattgg aatcacacga cctggatgga
     9661 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     9721 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     9781 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     9841 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     9901 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
     9961 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
    10021 cggatctcga cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
    10081 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
    10141 aaaaattcaa aattttatc
//
