LOCUS       Exported                9622 bp ds-DNA     circular SYN 01-SEP-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9622)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 7, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9622
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        10..385
                     /label=TRE3G promoter
     primer_bind     361..414
                     /label=lentiTO_HA_Fw
     CDS             395..421
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    428..1863
                     /label=PPM1H
     misc_feature    1871..1987
                     /label=mito tag
     primer_bind     complement(1966..2004)
                     /label=lentiTO_mito_rv
     primer_bind     1994..2013
                     /label=lentiTO_HA_Fw
     misc_feature    2030..2575
                     /label=EF1 promoter
     misc_feature    2575..2576
                     /label=GCTAGAC
                     /note="Geneious type: Editing History Deletion"
     gene            2582..3325
                     /label=rtta3G
     CDS             3335..3391
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     gene            3392..3790
                     /label=Blasticidin Resistance
     misc_feature    3392
                     /label=ATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTT...
                     /note="Geneious type: Editing History Deletion"
     misc_feature    3797..4386
                     /label=WPRE
     misc_feature    4160..4161
                     /label=G
                     /note="Geneious type: Editing History Deletion"
     misc_feature    complement(6237..7097)
                     /label=AmpR (beta-lactamase)
                     /note="AmpR (beta-lactamase)"
     misc_feature    8780..9012
                     /label=RRE
     misc_feature    9013..9501
                     /label=Env
     misc_feature    9013..9501
                     /label=Env
ORIGIN
        1 gatactagtt ttactcccta tcagtgatag agaacgtatg aagagtttac tccctatcag
       61 tgatagagaa cgtatgcaga ctttactccc tatcagtgat agagaacgta taaggagttt
      121 actccctatc agtgatagag aacgtatgac cagtttactc cctatcagtg atagagaacg
      181 tatctacagt ttactcccta tcagtgatag agaacgtata tccagtttac tccctatcag
      241 tgatagagaa cgtataagct ttaggcgtgt acggtgggcg cctataaaag cagagctcgt
      301 ttagtgaacc gtcagatcgc ctggagcaat tccacaacac ttttgtctta taccaacttt
      361 ccgtaccact tcctaccctc gtaaatctag aatgtaccca tacgatgtgc cagattacgc
      421 cggatcccgt ttcccctacg ggcggccaga gttcctgggg ctgtctcagg acgaggtgga
      481 gtgcagcgcc gaccacatcg cccgccccat cctcatcctc aaggagactc ggcggctgcc
      541 ctgggccact ggctacgcag aggttatcaa tgccgggaag agcacacaca atgaagacca
      601 agccagctgt gaggtgctca ctgtgaagaa gaaggcaggg gccgtgacct caaccccaaa
      661 caggaactca tccaagagac ggtcctccct tcccaatggg gaagggctgc agctgaagga
      721 gaactcggaa tccgagggtg tttcctgcca ctattggtcg ctgtttgacg ggcacgcggg
      781 gtccggggcc gcggtggtgg cgtcacgcct gctgcagcac cacatcacgg agcagctgca
      841 ggacatcgtg gacatcctga agaactccgc cgtcctgccc cctacctgcc tgggggagga
      901 gcctgagaac acgcccgcca acagccggac tctgacccgg gcagcctccc tgcgcggagg
      961 ggtgggggcc ccgggctccc ccagcacgcc ccccacacgc ttctttaccg agaagaagat
     1021 tccccatgag tgcctggtca tcggagcgct tgaaagtgca ttcaaggaaa tggacctaca
     1081 gatagaacga gagaggagtt catataatat atctggtggc tgcacggccc tcattgtgat
     1141 ttgccttttg gggaagctgt atgttgcaaa tgctggggat agcagggcca taatcatcag
     1201 aaatggagaa attatcccca tgtcttcaga atttaccccc gagacggagc gccagcgact
     1261 tcagtacctg gcattcatgc agcctcactt gctgggaaat gagttcacac atttggagtt
     1321 tccaaggaga gtacagagaa aggagcttgg aaagaagatg ctctacaggg actttaatat
     1381 gacaggctgg gcatacaaaa ccattgagga tgaggacttg aagttccccc ttatatatgg
     1441 agaaggcaag aaggcccggg taatggcaac tattggagtg accaggggac ttggggacca
     1501 tgacctgaag gtgcatgact ccaacatcta cattaaacca ttcctgtctt cagctccaga
     1561 ggtaagaatc tacgatcttt caaaatatga tcatggatca gatgatgtgc tgatcttggc
     1621 cactgatgga ctctgggacg ttttatcaaa tgaagaagta gcagaagcaa tcactcagtt
     1681 tcttcctaac tgtgatccag atgatcctca caggtacaca ctggcagctc aggacctggt
     1741 gatgcgtgcc cggggtgtgc tgaaggacag aggatggcgg atatctaatg accgactggg
     1801 ctcaggagac gacatttctg tatatgtcat tcctttaata catggaaaca agctgtcatc
     1861 agcggccgcc ttctgggaaa ggaacctgcc ctctgtttct ggcctgctga agatcattgg
     1921 attttccaca tcagtaactg ccctggggtt tgtgctgtac aaatacaagc tcctgccacg
     1981 gtcttgagct agctaccctt acgacgtgcc agactacgcc taaggccgca aggatctgcg
     2041 atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2101 ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta aactgggaaa
     2161 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2221 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca cagctgaagc
     2281 ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc cgccatccac
     2341 gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg
     2401 tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc ccttggagcc
     2461 tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa ctctacgtct
     2521 ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg cctacgccac
     2581 catgagccgg ctggacaaga gcaaagtgat caacagcgcc ctggaactgc tgaacggcgt
     2641 gggcatcgag ggcctgacca cccggaagct ggcccagaaa ctgggcgtgg aacagcccac
     2701 cctgtactgg cacgtgaaga acaagcgggc cctgctggac gccctgccca tcgagatgct
     2761 ggaccggcac cacacccaca gctgccctct ggaaggcgag agctggcagg acttcctgcg
     2821 gaacaacgcc aagagctaca gatgcgccct gctgagccac cgggacggcg ccaaagtgca
     2881 cctgggcacc agacccaccg agaagcagta cgagacactg gaaaaccagc tggccttcct
     2941 gtgccagcag ggcttcagcc tggaaaacgc cctgtacgcc ctgagcgccg tgggccactt
     3001 caccctgggc tgcgtgctgg aagaacagga acaccaggtc gccaaagagg aaagagagac
     3061 acccaccacc gacagcatgc cccccctgct gaagcaggcc atcgagctgt tcgacagaca
     3121 gggcgccgag cccgccttcc tgttcggcct ggaactgatc atctgcggcc tcgagaagca
     3181 gctgaagtgc gagagcggcg gacccaccga cgccctggac gacttcgacc tggacatgct
     3241 gcccgccgat gccctggatg attttgatct ggatatgctc cctgctgacg ctctcgatga
     3301 cttcgatctc gatatgctgc caggcggaag cggagctact aacttcagcc tgctgaagca
     3361 ggctggagac gtggaggaga accctggacc tatggccaag cctttgtctc aagaagaatc
     3421 caccctcatt gaaagagcaa cggctacaat caacagcatc cccatctctg aagactacag
     3481 cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc actggtgtca atgtatatca
     3541 ttttactggg ggaccttgtg cagaactcgt ggtgctgggc actgctgctg ctgcggcagc
     3601 tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac aggggcatct tgagcccctg
     3661 cggacggtgc cgacaggtgc ttctcgatct gcatcctggg atcaaagcca tagtgaagga
     3721 cagtgatgga cagccgacgg cagttgggat tcgtgaattg ctgccctctg gttatgtgtg
     3781 ggagggctaa gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     3841 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     3901 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     3961 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     4021 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     4081 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     4141 gacaggggct cggctgttgg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     4201 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     4261 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     4321 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     4381 ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     4441 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaaaataag atctgctttt
     4501 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     4561 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     4621 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     4681 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     4741 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     4801 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     4861 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     4921 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     4981 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     5041 gacttttgca gagacggccc aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     5101 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     5161 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     5221 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     5281 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     5341 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     5401 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     5461 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     5521 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     5581 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     5641 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     5701 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     5761 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     5821 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     5881 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     5941 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     6001 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     6061 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     6121 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     6181 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     6241 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     6301 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     6361 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     6421 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     6481 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     6541 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     6601 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     6661 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     6721 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     6781 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     6841 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     6901 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     6961 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     7021 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     7081 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     7141 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     7201 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     7261 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
     7321 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     7381 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     7441 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     7501 cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac
     7561 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     7621 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     7681 acgacggcca gtgccaagct gacgcgtgta gtcttatgca atactcttgt agtcttgcaa
     7741 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     7801 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     7861 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
     7921 tcgatacaat aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7981 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     8041 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     8101 ggaaaatctc tagcagtggc gcccgaacag ggacctgaaa gcgaaaggga aaccagagga
     8161 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     8221 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     8281 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     8341 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     8401 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     8461 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     8521 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     8581 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggcca ctgatcttca
     8641 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     8701 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     8761 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     8821 ctatgggcgc agcctcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     8881 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     8941 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     9001 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     9061 cttggaatgc tagttggagt aataaatctc tggaacagat tggaatcaca cgacctggat
     9121 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     9181 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     9241 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     9301 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     9361 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     9421 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     9481 gaacggatct cgacggttaa cttttaaaag aaaagggggg attggggggt acagtgcagg
     9541 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     9601 tacaaaaatt caaaatttta tc
//
