LOCUS       LCCV2-Lum-sgMre1       10527 bp DNA     circular SYN 01-SEP-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 1, 2023 from SnapGene 7.0.0
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.1.0
FEATURES             Location/Qualifiers
     source          1..10527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      929..1517
                     /label=rori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
                     /note="color: #ffff00"
     CDS             1700..1792
                     /codon_start=1
                     /label=rlacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="APNTQTASPRALADSLMQLARQVSRLESGQ"
     protein_bind    1805..1826
                     /label=rCAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
                     /note="color: #31849b"
     promoter        1841..1871
                     /label=rlac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff"
     protein_bind    1879..1895
                     /label=rlac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
                     /note="color: #31849b"
     polyA_signal    complement(1968..2089)
                     /label=rSV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
                     /note="color: #a6acb3; direction: LEFT"
     CDS             complement(2084..2209)
                     /codon_start=1
                     /label=rble (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=97.6"
                     /note="/match_length=33.3"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="RDFDSTAAFYERLGFGIVFRDAGWMILQRGDLMLEFFAHPNL"
     CDS             complement(2219..2593)
                     /codon_start=1
                     /label=rBleoR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(2612..2659)
                     /label=rEM7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     promoter        complement(2707..3036)
                     /label=rSV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      complement(3050..3478)
                     /direction=LEFT
                     /label=rf1 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
                     /note="color: #ffff00"
     promoter        complement(3499..3520)
                     /label=rSV40 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=6.1"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     polyA_signal    complement(3524..3748)
                     /label=rbGH poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
                     /note="color: #a6acb3; direction: LEFT"
     LTR             complement(3780..4013)
                     /label=r3' LTR (Delta-U3)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
                     /note="color: #ffe4c4; direction: LEFT"
     CDS             complement(4015..4089)
                     /codon_start=1
                     /label=rnef (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=80.0"
                     /note="/match_length=25.8"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="PASTLRPMTYKAAVDLSHFLKEKGG"
     misc_feature    complement(4085..4673)
                     /label=rWPRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3; direction: LEFT"
     CDS             complement(4689..5204)
                     /codon_start=1
                     /label=rNluc
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             complement(5220..5936)
                     /codon_start=1
                     /label=rEGFP
                     /note="pLannotate"
                     /note="/database=fpbase"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(5994..7021)
                     /codon_start=1
                     /label=rCre (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.8"
                     /note="/match_length=99.6"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="CPIY*PYTKICLHYRSMQRVMRFART*WTCSGIARRFLSIPGKCF
                     SPFAGRGRHGAS*ITGNGFPQNLKMFAIIFYIFRRAVWQ*KLSSNIWAS*TCFIVGPGC
                     HDQVTAMLFHWLCGGSEKKTLMPVNVQNRL*RSNALISTRFVHSWKIAIAARIYVIWHF
                     WGLLITPCYV*PKLPGSGLKISHVLTVGEC*SILAERKRWLAPQV*RRHLAWG*LNWSS
                     DGFPYLV*LMIRITTCFAGSEKMVLPRHLPPASYQLAPWKGFLKQLID*FTALRMTLVR
                     DTWPGLDTVPVSEPREIWPALEFQYRRSCKLVAGPM*ILS*TISVTWIVKQGQWCACWK
                     MA"
     promoter        complement(7066..7277)
                     /label=rEF-1-alpha core promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.1"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     CDS             complement(7303..7321)
                     /codon_start=1
                     /label=rsmall t antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.6"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="LGLERS"
     misc_RNA        complement(7339..7414)
                     /label=rgRNA scaffold
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_RNA"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    7415..7434
                     /label=sgMRE11
                     /note="color: #ff9900"
     promoter        complement(7444..7684)
                     /label=rU6 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    complement(7735..7852)
                     /label=rcPPT/CTS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3; direction: LEFT"
     CDS             complement(7902..8002)
                     /codon_start=1
                     /label=rRev (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.0"
                     /note="/match_length=28.8"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="THLPTPRGPDRPEGIEEEGGERDRDRSIRLVNG"
     misc_feature    complement(8379..8612)
                     /label=rRRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3; direction: LEFT"
     CDS             complement(8758..9121)
                     /codon_start=1
                     /label=rHIV-1 gag (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.3"
                     /note="/match_length=24.2"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MGARASVLSGGELDRDGKKFG*GQGERKNIN*NI*YGQAGS*NDS
                     QLILAC*KHQKAVDKYWDSYNHPFRQDQKNLDHYIIQ*QPSIVCIKG*R*KTPRKL*TR
                     *RKSKTKVRPPHSKRPL"
     misc_feature    complement(9105..9230)
                     /label=rHIV-1 Psi
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3; direction: LEFT"
     LTR             complement(9277..9457)
                     /label=r5' LTR (truncated)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
                     /note="color: #ffe4c4; direction: LEFT"
     promoter        complement(9475..9674)
                     /label=rCMV promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=98.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     enhancer        complement(9675..10054)
                     /label=rCMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    10209..10261
                     /label=rbom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=37.6"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3"
     promoter        10320..10424
                     /label=rAmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             join(10425..10527,1..758)
                     /codon_start=1
                     /label=rAmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
       61 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
      121 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
      181 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
      241 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
      301 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
      361 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
      421 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
      481 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
      541 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
      601 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
      661 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
      721 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
      781 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
      841 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
      901 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
      961 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     1021 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     1081 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     1141 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     1201 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     1261 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     1321 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     1381 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     1441 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     1501 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     1561 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     1621 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     1681 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     1741 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     1801 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     1861 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     1921 atgattacgc caagctctag ctagaggtcg acggtataca gacatgataa gatacattga
     1981 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     2041 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagttg gggtgggcga
     2101 agaactccag catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga
     2161 ttccgaagcc caacctttca tagaaggcgg cggtggaatc gaaatctcgt agcacgtgtc
     2221 agtcctgctc ctcggccacg aagtgcacgc agttgccggc cgggtcgcgc agggcgaact
     2281 cccgccccca cggctgctcg ccgatctcgg tcatggccgg cccggaggcg tcccggaagt
     2341 tcgtggacac gacctccgac cactcggcgt acagctcgtc caggccgcgc acccacaccc
     2401 aggccagggt gttgtccggc accacctggt cctggaccgc gctgatgaac agggtcacgt
     2461 cgtcccggac cacaccggcg aagtcgtcct ccacgaagtc ccgggagaac ccgagccggt
     2521 cggtccagaa ctcgaccgct ccggcgacgt cgcgcgcggt gagcaccgga acggcactgg
     2581 tcaacttggc catggtttag ttcctcacct tgtcgtatta tactatgccg atatactatg
     2641 ccgatgatta attgtcaaca cgtgctgatc agatccgaaa atggatatac aagctcccgg
     2701 gagctttttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc
     2761 agaggcagag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg
     2821 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat
     2881 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
     2941 ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
     3001 ggagcctggg gactttccac accctaactg acacacattc cacagaatta attcgcgtta
     3061 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     3121 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     3181 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     3241 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     3301 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     3361 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     3421 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtgg
     3481 ggataccccc tagagcccca gctggttctt tccgcctcag aagccataga gcccaccgca
     3541 tccccagcat gcctgctatt gtcttcccaa tcctccccct tgctgtcctg ccccacccca
     3601 ccccccagaa tagaatgaca cctactcaga caatgcgatg caatttcctc attttattag
     3661 gaaaggacag tgggagtggc accttccagg gtcaaggaag gcacggggga ggggcaaaca
     3721 acagatggct ggcaactaga aggcacagtc gaggctgatc agcgggttta aacgggccct
     3781 gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac cagagtcaca
     3841 caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag gcttaagcag
     3901 tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc agagagaccc
     3961 agtacaagca aaaagcagat cttgtcttcg ttgggagtga attagccctt ccagtccccc
     4021 cttttctttt aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagt
     4081 cgacgcgggg aggcggccca aagggagatc cgactcgtct gagggcgaag gcgaagacgc
     4141 ggaagaggcc gcagagccgg cagcaggccg cgggaaggaa ggtccgctgg attgagggcc
     4201 gaagggacgt agcagaagga cgtcccgcgc agaatccagg tggcaacaca ggcgagcagc
     4261 caaggaaagg acgatgattt ccccgacaac accacggaat tgtcagtgcc caacagccga
     4321 gcccctgtcc agcagcgggc aaggcaggcg gcgatgagtt ccgccgtggc aatagggagg
     4381 gggaaagcga aagtcccgga aaggagctga caggtggtgg caatgcccca accagtgggg
     4441 gttgcgtcag caaacacagt gcacaccacg ccacgttgcc tgacaacggg ccacaactcc
     4501 tcataaagag acagcaacca ggatttatac aaggaggaga aaatgaaagc catacgggaa
     4561 gcaatagcat gatacaaagg cattaaagca gcgtatccac atagcgtaaa aggagcaaca
     4621 tagttaagaa taccagtcaa tctttcacaa attttgtaat ccagaggttg attgtcgact
     4681 taacgcgttt acgccagaat gcgttcgcac agccgccagc cggtcactcc gttgatggtt
     4741 actcggaaca gcagggagcc gtcggggttg atcaggcgct cgtcgataat tttgttgccg
     4801 ttccacaggg tccctgttac agtgatcttt ttgccgtcga acacggcgat gccttcatac
     4861 ggccgtccga aatagtcgat catgttcggc gtaaccccgt cgattaccag tgtgccatag
     4921 tgcaggatca ccttaaagtg atgatcatcc acagggtaca ccaccttaaa aattttttcg
     4981 atctggccca tttggtcgcc gctcagacct tcatacggga tgatgacatg gatgtcgatc
     5041 ttcagcccat tttcaccgct caggacaatc ctttggatcg gagttacgga caccccgaga
     5101 ttctgaaaca aactggacac acctccctgt tcaaggactt ggtccaggtt gtagccggct
     5161 gtctgtcgcc agtccccaac gaaatcttcg agtgtgaaga ccatacctcc ggagatatcc
     5221 ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc
     5281 atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg actgggtgct caggtagtgg
     5341 ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg
     5401 agctgcacgc tgccgtcctc gatgttgtgg cggatcttga agttcacctt gatgccgttc
     5461 ttctgcttgt cggccatgat atagacgttg tggctgttgt agttgtactc cagcttgtgc
     5521 cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg
     5581 gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag
     5641 atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc
     5701 atgtggtcgg ggtagcggct gaagcactgc acgccgtagg tcagggtggt cacgagggtg
     5761 ggccagggca cgggcagctt gccggtggtg cagatgaact tcagggtcag cttgccgtag
     5821 gtggcatcgc cctcgccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc
     5881 agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct caccatcggt
     5941 ccaggattct cttcgacatc tccggcttgt ttcagcagag agaagtttgt tgcatcgcca
     6001 tcttccagca ggcgcaccat tgcccctgtt tcactatcca ggttacggat atagttcatg
     6061 acaatattta cattggtcca gccaccagct tgcatgatct ccggtattga aactccagcg
     6121 cgggccatat ctcgcgcggc tccgacacgg gcactgtgtc cagaccaggc caggtatctc
     6181 tgaccagagt catccttagc gccgtaaatc aatcgatgag ttgcttcaaa aatcccttcc
     6241 agggcgcgag ttgatagctg gctggtggca gatggcgcgg caacaccatt ttttctgacc
     6301 cggcaaaaca ggtagttatt cggatcatca gctacaccag atacggaaat ccatcgctcg
     6361 accagtttag ttacccccag gctaagtgcc ttctctacac ctgcggtgct aaccagcgtt
     6421 ttcgttctgc caatatggat taacattctc ccaccgtcag tacgtgagat atctttaacc
     6481 ctgatcctgg caatttcggc tatacgtaac agggtgttat aagcaatccc cagaaatgcc
     6541 agattacgta tatcctggca gcgatcgcta ttttccatga gtgaacgaac ctggtcgaaa
     6601 tcagtgcgtt cgaacgctag agcctgtttt gcacgttcac cggcatcaac gttttctttt
     6661 cggatccgcc gcataaccag tgaaacagca ttgctgtcac ttggtcgtgg cagcccggac
     6721 cgacgatgaa gcatgtttag ctggcccaaa tgttgctgga tagtttttac tgccagaccg
     6781 cgcgcctgaa gatatagaag ataatcgcga acatcttcag gttctgcggg aaaccatttc
     6841 cggttattca acttgcacca tgccgcccac gaccggcaaa cggactgaag cattttccag
     6901 gtatgctcag aaaacgcctg gcgatccctg aacatgtcca tcaggttctt gcgaacctca
     6961 tcactcgttg catcgaccgg taatgcaggc aaattttggt gtacggtcag taaattggac
     7021 accttcctct tcttcttggg catggtagat cctctagaac cggtcctgtg ttctggcggc
     7081 aaacccgttg cgaaaaagaa cgttcacggc gactactgca cttatatacg gttctccccc
     7141 accctcggga aaaaggcgga gccagtacac gacatcactt tcccagttta ccccgcgcca
     7201 ccttctctag gcaccggatc aattgccgac ccctcccccc aacttctcgg ggactgtggg
     7261 cgatgtgcgc tctgcccact gacgggcacc ggagccaatt cccactcctt tcaagaccta
     7321 gctagcgaat tcaaaaaagc accgactcgg tgccactttt tcaagttgat aacggactag
     7381 ccttatttta acttgctatt tctagctcta aaacgcctcg agtagtgatc tccacggtgt
     7441 ttcgtccttt ccacaagata tataaagcca agaaatcgaa atactttcaa gttacggtaa
     7501 gcatatgata gtccatttta aaacataatt ttaaaactgc aaactaccca agaaattatt
     7561 actttctacg tcacgtattt tgtactaata tctttgtgtt tacagtcaaa ttaattctaa
     7621 ttatctctct aacagccttg tatcgtatat gcaaatatga aggaatcatg ggaaataggc
     7681 cctcggtacc ttaattaacc aaactggatc tctgctgtcc ctgtaataaa cccgaaaatt
     7741 ttgaattttt gtaatttgtt tttgtaattc tttagtttgt atgtctgttg ctattatgtc
     7801 tactattctt tcccctgcac tgtacccccc aatcccccct tttcttttaa aattgtggat
     7861 gaatactgcc atttgtctgc agaattggcg cacgcagtgc cgatccgttc actaatcgaa
     7921 tggatctgtc tctgtctctc tctccacctt cttcttctat tccttcgggc ctgtcgggtc
     7981 ccctcggggt tgggaggtgg gtctgaaacg ataatggtga atatccctgc ctaactctat
     8041 tcactataga aagtacagca aaaactattc ttaaacctac caagcctcct actatcatta
     8101 tgaataattt tatataccac agccaatttg ttatgttaaa ccaattccac aaacttgccc
     8161 atttatctaa ttccaataat tcttgttcat tcttttcttg ctggttttgc gattcttcaa
     8221 ttaaggagtg tattaagctt gtgtaattgt taatttctct gtcccactcc atccaggtcg
     8281 tgtgattcca aatctgttcc agagatttat tactccaact agcattccaa ggcacagcag
     8341 tggtgcaaat gagttttcca gagcaacccc aaatccccag gagctgttga tcctttaggt
     8401 atctttccac agccaggatt cttgcctgga gctgcttgat gccccagact gtgagttgca
     8461 acagatgctg ttgcgcctca atagccctca gcaaattgtt ctgctgctgc actataccag
     8521 acaataattg tctggcctgt accgtcagcg tcattgacgc tgcgcccata gtgcttcctg
     8581 ctgctcccaa gaacccaagg aacaaagctc ctattcccac tgctcttttt tctctctgca
     8641 ccactcttct ctttgccttg gtgggtgcta ctcctaatgg ttcaattttt actactttat
     8701 atttatataa ttcacttctc caattgtccc tcatatctcc tcctccaggt ctgaagatca
     8761 gcggccgctt gctgtgcggt ggtcttactt ttgttttgct cttcctctat cttgtctaaa
     8821 gcttccttgg tgtcttttat ctctatcctt tgatgcacac aatagagggt tgctactgta
     8881 ttatataatg atctaagttc ttctgatcct gtctgaaggg atggttgtag ctgtcccagt
     8941 atttgtctac agccttctga tgtttctaac aggccaggat taactgcgaa tcgttctagc
     9001 tccctgcttg cccatactat atgttttaat ttatattttt tctttccccc tggccttaac
     9061 cgaatttttt cccatcgcga tctaattctc ccccgcttaa tactgacgct ctcgcaccca
     9121 tctctctcct tctagcctcc gctagtcaaa atttttggcg tactcaccag tcgccgcccc
     9181 tcgcctcttg ccgtgcgcgc ttcagcaagc cgagtcctgc gtcgagagag ctcctctggt
     9241 ttccctttcg ctttcaagtc cctgttcggg cgccactgct agagattttc cacactgact
     9301 aaaagggtct gagggatctc tagttaccag agtcacacaa cagacgggca cacactactt
     9361 gaagcactca aggcaagctt tattgaggct taagcagtgg gttccctagt tagccagaga
     9421 gctcccaggc tcagatctgg tctaaccaga gagacccagt acaggcaaaa cgcgctgctt
     9481 atatagacct cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta
     9541 cgacattttg gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg
     9601 gggtggagac ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc
     9661 caaaaccgca tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga
     9721 aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg
     9781 tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac
     9841 cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca
     9901 tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt
     9961 accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt
    10021 gattactatt aataactagt caataatcaa tgtcaacgcg tatatctggc ccgtacatcg
    10081 cgaagcagcg caaaacgcct aaccctaagc agattcttca tgcaattgtc ggtcaagcct
    10141 tgccttgttg tagcttaaat tttgctcgcg cactactcag cgacctccaa cacacaagca
    10201 gggagcagat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
    10261 aggggatcgg gagatctccc gatccgtcga cgtcaggtgg cacttttcgg ggaaatgtgc
    10321 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
    10381 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
    10441 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
    10501 aaacgctggt gaaagtaaaa gatgctg
//
