LOCUS       LCCV2-Lum-sgZBP1       10527 bp DNA     circular SYN 01-SEP-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 1, 2023 from SnapGene 7.0.0
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.1.0
FEATURES             Location/Qualifiers
     source          1..10527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      920..1508
                     /label=rori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
                     /note="color: #ffff00"
     CDS             1691..1783
                     /codon_start=1
                     /label=rlacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="APNTQTASPRALADSLMQLARQVSRLESGQ"
     protein_bind    1796..1817
                     /label=rCAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
                     /note="color: #31849b"
     promoter        1832..1862
                     /label=rlac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff"
     protein_bind    1870..1886
                     /label=rlac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
                     /note="color: #31849b"
     polyA_signal    complement(1959..2080)
                     /label=rSV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
                     /note="color: #a6acb3; direction: LEFT"
     CDS             complement(2075..2200)
                     /codon_start=1
                     /label=rble (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=97.6"
                     /note="/match_length=33.3"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="RDFDSTAAFYERLGFGIVFRDAGWMILQRGDLMLEFFAHPNL"
     CDS             complement(2210..2584)
                     /codon_start=1
                     /label=rBleoR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(2603..2650)
                     /label=rEM7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     promoter        complement(2698..3027)
                     /label=rSV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      complement(3041..3469)
                     /direction=LEFT
                     /label=rf1 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
                     /note="color: #ffff00"
     promoter        complement(3490..3511)
                     /label=rSV40 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=6.1"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     polyA_signal    complement(3515..3739)
                     /label=rbGH poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
                     /note="color: #a6acb3; direction: LEFT"
     LTR             complement(3771..4004)
                     /label=r3' LTR (Delta-U3)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
                     /note="color: #ffe4c4; direction: LEFT"
     CDS             complement(4006..4080)
                     /codon_start=1
                     /label=rnef (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=80.0"
                     /note="/match_length=25.8"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="PASTLRPMTYKAAVDLSHFLKEKGG"
     misc_feature    complement(4076..4664)
                     /label=rWPRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3; direction: LEFT"
     CDS             complement(4680..5195)
                     /codon_start=1
                     /label=rNluc
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             complement(5211..5927)
                     /codon_start=1
                     /label=rEGFP
                     /note="pLannotate"
                     /note="/database=fpbase"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(5985..7012)
                     /codon_start=1
                     /label=rCre (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.8"
                     /note="/match_length=99.6"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="CPIY*PYTKICLHYRSMQRVMRFART*WTCSGIARRFLSIPGKCF
                     SPFAGRGRHGAS*ITGNGFPQNLKMFAIIFYIFRRAVWQ*KLSSNIWAS*TCFIVGPGC
                     HDQVTAMLFHWLCGGSEKKTLMPVNVQNRL*RSNALISTRFVHSWKIAIAARIYVIWHF
                     WGLLITPCYV*PKLPGSGLKISHVLTVGEC*SILAERKRWLAPQV*RRHLAWG*LNWSS
                     DGFPYLV*LMIRITTCFAGSEKMVLPRHLPPASYQLAPWKGFLKQLID*FTALRMTLVR
                     DTWPGLDTVPVSEPREIWPALEFQYRRSCKLVAGPM*ILS*TISVTWIVKQGQWCACWK
                     MA"
     promoter        complement(7057..7268)
                     /label=rEF-1-alpha core promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.1"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     CDS             complement(7294..7312)
                     /codon_start=1
                     /label=rsmall t antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.6"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="LGLERS"
     misc_RNA        complement(7330..7405)
                     /label=rgRNA scaffold
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_RNA"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    7406..7425
                     /label=sgZBP1#1
                     /note="color: #ff9900"
     promoter        complement(7435..7675)
                     /label=rU6 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    complement(7726..7843)
                     /label=rcPPT/CTS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3; direction: LEFT"
     CDS             complement(7893..7993)
                     /codon_start=1
                     /label=rRev (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.0"
                     /note="/match_length=28.8"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="THLPTPRGPDRPEGIEEEGGERDRDRSIRLVNG"
     misc_feature    complement(8370..8603)
                     /label=rRRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3; direction: LEFT"
     CDS             complement(8749..9112)
                     /codon_start=1
                     /label=rHIV-1 gag (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.3"
                     /note="/match_length=24.2"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MGARASVLSGGELDRDGKKFG*GQGERKNIN*NI*YGQAGS*NDS
                     QLILAC*KHQKAVDKYWDSYNHPFRQDQKNLDHYIIQ*QPSIVCIKG*R*KTPRKL*TR
                     *RKSKTKVRPPHSKRPL"
     misc_feature    complement(9096..9221)
                     /label=rHIV-1 Psi
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3; direction: LEFT"
     LTR             complement(9268..9448)
                     /label=r5' LTR (truncated)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
                     /note="color: #ffe4c4; direction: LEFT"
     promoter        complement(9466..9665)
                     /label=rCMV promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=98.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     enhancer        complement(9666..10045)
                     /label=rCMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    10200..10252
                     /label=rbom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=37.6"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3"
     promoter        10311..10415
                     /label=rAmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             join(10416..10527,1..749)
                     /codon_start=1
                     /label=rAmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
       61 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      121 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      181 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      241 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      301 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      361 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      421 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      481 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      541 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      601 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      661 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      721 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
      781 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
      841 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
      901 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      961 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1021 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1081 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1141 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1201 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1261 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1321 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1381 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1441 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1501 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1561 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1621 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1681 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1741 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     1801 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     1861 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     1921 ccaagctcta gctagaggtc gacggtatac agacatgata agatacattg atgagtttgg
     1981 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     2041 tgctttattt gtaaccatta taagctgcaa taaacaagtt ggggtgggcg aagaactcca
     2101 gcatgagatc cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc
     2161 ccaacctttc atagaaggcg gcggtggaat cgaaatctcg tagcacgtgt cagtcctgct
     2221 cctcggccac gaagtgcacg cagttgccgg ccgggtcgcg cagggcgaac tcccgccccc
     2281 acggctgctc gccgatctcg gtcatggccg gcccggaggc gtcccggaag ttcgtggaca
     2341 cgacctccga ccactcggcg tacagctcgt ccaggccgcg cacccacacc caggccaggg
     2401 tgttgtccgg caccacctgg tcctggaccg cgctgatgaa cagggtcacg tcgtcccgga
     2461 ccacaccggc gaagtcgtcc tccacgaagt cccgggagaa cccgagccgg tcggtccaga
     2521 actcgaccgc tccggcgacg tcgcgcgcgg tgagcaccgg aacggcactg gtcaacttgg
     2581 ccatggttta gttcctcacc ttgtcgtatt atactatgcc gatatactat gccgatgatt
     2641 aattgtcaac acgtgctgat cagatccgaa aatggatata caagctcccg ggagcttttt
     2701 gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggcaga
     2761 ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc
     2821 ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga
     2881 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
     2941 cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
     3001 ggactttcca caccctaact gacacacatt ccacagaatt aattcgcgtt aaatttttgt
     3061 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     3121 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     3181 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     3241 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     3301 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     3361 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     3421 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtg gggatacccc
     3481 ctagagcccc agctggttct ttccgcctca gaagccatag agcccaccgc atccccagca
     3541 tgcctgctat tgtcttccca atcctccccc ttgctgtcct gccccacccc accccccaga
     3601 atagaatgac acctactcag acaatgcgat gcaatttcct cattttatta ggaaaggaca
     3661 gtgggagtgg caccttccag ggtcaaggaa ggcacggggg aggggcaaac aacagatggc
     3721 tggcaactag aaggcacagt cgaggctgat cagcgggttt aaacgggccc tgctagagat
     3781 tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg
     3841 ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc
     3901 tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc cagtacaagc
     3961 aaaaagcaga tcttgtcttc gttgggagtg aattagccct tccagtcccc ccttttcttt
     4021 taaaaagtgg ctaagatcta cagctgcctt gtaagtcatt ggtcttaaag tcgacgcggg
     4081 gaggcggccc aaagggagat ccgactcgtc tgagggcgaa ggcgaagacg cggaagaggc
     4141 cgcagagccg gcagcaggcc gcgggaagga aggtccgctg gattgagggc cgaagggacg
     4201 tagcagaagg acgtcccgcg cagaatccag gtggcaacac aggcgagcag ccaaggaaag
     4261 gacgatgatt tccccgacaa caccacggaa ttgtcagtgc ccaacagccg agcccctgtc
     4321 cagcagcggg caaggcaggc ggcgatgagt tccgccgtgg caatagggag ggggaaagcg
     4381 aaagtcccgg aaaggagctg acaggtggtg gcaatgcccc aaccagtggg ggttgcgtca
     4441 gcaaacacag tgcacaccac gccacgttgc ctgacaacgg gccacaactc ctcataaaga
     4501 gacagcaacc aggatttata caaggaggag aaaatgaaag ccatacggga agcaatagca
     4561 tgatacaaag gcattaaagc agcgtatcca catagcgtaa aaggagcaac atagttaaga
     4621 ataccagtca atctttcaca aattttgtaa tccagaggtt gattgtcgac ttaacgcgtt
     4681 tacgccagaa tgcgttcgca cagccgccag ccggtcactc cgttgatggt tactcggaac
     4741 agcagggagc cgtcggggtt gatcaggcgc tcgtcgataa ttttgttgcc gttccacagg
     4801 gtccctgtta cagtgatctt tttgccgtcg aacacggcga tgccttcata cggccgtccg
     4861 aaatagtcga tcatgttcgg cgtaaccccg tcgattacca gtgtgccata gtgcaggatc
     4921 accttaaagt gatgatcatc cacagggtac accaccttaa aaattttttc gatctggccc
     4981 atttggtcgc cgctcagacc ttcatacggg atgatgacat ggatgtcgat cttcagccca
     5041 ttttcaccgc tcaggacaat cctttggatc ggagttacgg acaccccgag attctgaaac
     5101 aaactggaca cacctccctg ttcaaggact tggtccaggt tgtagccggc tgtctgtcgc
     5161 cagtccccaa cgaaatcttc gagtgtgaag accatacctc cggagatatc cttgtacagc
     5221 tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg
     5281 cgcttctcgt tggggtcttt gctcagggcg gactgggtgc tcaggtagtg gttgtcgggc
     5341 agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg
     5401 ctgccgtcct cgatgttgtg gcggatcttg aagttcacct tgatgccgtt cttctgcttg
     5461 tcggccatga tatagacgtt gtggctgttg tagttgtact ccagcttgtg ccccaggatg
     5521 ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc
     5581 tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc
     5641 tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt catgtggtcg
     5701 gggtagcggc tgaagcactg cacgccgtag gtcagggtgg tcacgagggt gggccagggc
     5761 acgggcagct tgccggtggt gcagatgaac ttcagggtca gcttgccgta ggtggcatcg
     5821 ccctcgccct cgccggacac gctgaacttg tggccgttta cgtcgccgtc cagctcgacc
     5881 aggatgggca ccaccccggt gaacagctcc tcgcccttgc tcaccatcgg tccaggattc
     5941 tcttcgacat ctccggcttg tttcagcaga gagaagtttg ttgcatcgcc atcttccagc
     6001 aggcgcacca ttgcccctgt ttcactatcc aggttacgga tatagttcat gacaatattt
     6061 acattggtcc agccaccagc ttgcatgatc tccggtattg aaactccagc gcgggccata
     6121 tctcgcgcgg ctccgacacg ggcactgtgt ccagaccagg ccaggtatct ctgaccagag
     6181 tcatccttag cgccgtaaat caatcgatga gttgcttcaa aaatcccttc cagggcgcga
     6241 gttgatagct ggctggtggc agatggcgcg gcaacaccat tttttctgac ccggcaaaac
     6301 aggtagttat tcggatcatc agctacacca gatacggaaa tccatcgctc gaccagttta
     6361 gttaccccca ggctaagtgc cttctctaca cctgcggtgc taaccagcgt tttcgttctg
     6421 ccaatatgga ttaacattct cccaccgtca gtacgtgaga tatctttaac cctgatcctg
     6481 gcaatttcgg ctatacgtaa cagggtgtta taagcaatcc ccagaaatgc cagattacgt
     6541 atatcctggc agcgatcgct attttccatg agtgaacgaa cctggtcgaa atcagtgcgt
     6601 tcgaacgcta gagcctgttt tgcacgttca ccggcatcaa cgttttcttt tcggatccgc
     6661 cgcataacca gtgaaacagc attgctgtca cttggtcgtg gcagcccgga ccgacgatga
     6721 agcatgttta gctggcccaa atgttgctgg atagttttta ctgccagacc gcgcgcctga
     6781 agatatagaa gataatcgcg aacatcttca ggttctgcgg gaaaccattt ccggttattc
     6841 aacttgcacc atgccgccca cgaccggcaa acggactgaa gcattttcca ggtatgctca
     6901 gaaaacgcct ggcgatccct gaacatgtcc atcaggttct tgcgaacctc atcactcgtt
     6961 gcatcgaccg gtaatgcagg caaattttgg tgtacggtca gtaaattgga caccttcctc
     7021 ttcttcttgg gcatggtaga tcctctagaa ccggtcctgt gttctggcgg caaacccgtt
     7081 gcgaaaaaga acgttcacgg cgactactgc acttatatac ggttctcccc caccctcggg
     7141 aaaaaggcgg agccagtaca cgacatcact ttcccagttt accccgcgcc accttctcta
     7201 ggcaccggat caattgccga cccctccccc caacttctcg gggactgtgg gcgatgtgcg
     7261 ctctgcccac tgacgggcac cggagccaat tcccactcct ttcaagacct agctagcgaa
     7321 ttcaaaaaag caccgactcg gtgccacttt ttcaagttga taacggacta gccttatttt
     7381 aacttgctat ttctagctct aaaacccgtc atcgctcaac acctgcggtg tttcgtcctt
     7441 tccacaagat atataaagcc aagaaatcga aatactttca agttacggta agcatatgat
     7501 agtccatttt aaaacataat tttaaaactg caaactaccc aagaaattat tactttctac
     7561 gtcacgtatt ttgtactaat atctttgtgt ttacagtcaa attaattcta attatctctc
     7621 taacagcctt gtatcgtata tgcaaatatg aaggaatcat gggaaatagg ccctcggtac
     7681 cttaattaac caaactggat ctctgctgtc cctgtaataa acccgaaaat tttgaatttt
     7741 tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt gctattatgt ctactattct
     7801 ttcccctgca ctgtaccccc caatcccccc ttttctttta aaattgtgga tgaatactgc
     7861 catttgtctg cagaattggc gcacgcagtg ccgatccgtt cactaatcga atggatctgt
     7921 ctctgtctct ctctccacct tcttcttcta ttccttcggg cctgtcgggt cccctcgggg
     7981 ttgggaggtg ggtctgaaac gataatggtg aatatccctg cctaactcta ttcactatag
     8041 aaagtacagc aaaaactatt cttaaaccta ccaagcctcc tactatcatt atgaataatt
     8101 ttatatacca cagccaattt gttatgttaa accaattcca caaacttgcc catttatcta
     8161 attccaataa ttcttgttca ttcttttctt gctggttttg cgattcttca attaaggagt
     8221 gtattaagct tgtgtaattg ttaatttctc tgtcccactc catccaggtc gtgtgattcc
     8281 aaatctgttc cagagattta ttactccaac tagcattcca aggcacagca gtggtgcaaa
     8341 tgagttttcc agagcaaccc caaatcccca ggagctgttg atcctttagg tatctttcca
     8401 cagccaggat tcttgcctgg agctgcttga tgccccagac tgtgagttgc aacagatgct
     8461 gttgcgcctc aatagccctc agcaaattgt tctgctgctg cactatacca gacaataatt
     8521 gtctggcctg taccgtcagc gtcattgacg ctgcgcccat agtgcttcct gctgctccca
     8581 agaacccaag gaacaaagct cctattccca ctgctctttt ttctctctgc accactcttc
     8641 tctttgcctt ggtgggtgct actcctaatg gttcaatttt tactacttta tatttatata
     8701 attcacttct ccaattgtcc ctcatatctc ctcctccagg tctgaagatc agcggccgct
     8761 tgctgtgcgg tggtcttact tttgttttgc tcttcctcta tcttgtctaa agcttccttg
     8821 gtgtctttta tctctatcct ttgatgcaca caatagaggg ttgctactgt attatataat
     8881 gatctaagtt cttctgatcc tgtctgaagg gatggttgta gctgtcccag tatttgtcta
     8941 cagccttctg atgtttctaa caggccagga ttaactgcga atcgttctag ctccctgctt
     9001 gcccatacta tatgttttaa tttatatttt ttctttcccc ctggccttaa ccgaattttt
     9061 tcccatcgcg atctaattct cccccgctta atactgacgc tctcgcaccc atctctctcc
     9121 ttctagcctc cgctagtcaa aatttttggc gtactcacca gtcgccgccc ctcgcctctt
     9181 gccgtgcgcg cttcagcaag ccgagtcctg cgtcgagaga gctcctctgg tttccctttc
     9241 gctttcaagt ccctgttcgg gcgccactgc tagagatttt ccacactgac taaaagggtc
     9301 tgagggatct ctagttacca gagtcacaca acagacgggc acacactact tgaagcactc
     9361 aaggcaagct ttattgaggc ttaagcagtg ggttccctag ttagccagag agctcccagg
     9421 ctcagatctg gtctaaccag agagacccag tacaggcaaa acgcgctgct tatatagacc
     9481 tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt
     9541 ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga
     9601 cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc
     9661 atcaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat
     9721 aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg
     9781 ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac
     9841 tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt
     9901 attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt
     9961 tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat
    10021 taataactag tcaataatca atgtcaacgc gtatatctgg cccgtacatc gcgaagcagc
    10081 gcaaaacgcc taaccctaag cagattcttc atgcaattgt cggtcaagcc ttgccttgtt
    10141 gtagcttaaa ttttgctcgc gcactactca gcgacctcca acacacaagc agggagcaga
    10201 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca taggggatcg
    10261 ggagatctcc cgatccgtcg acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
    10321 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
    10381 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
    10441 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
    10501 tgaaagtaaa agatgctgaa gatcagt
//
