LOCUS       LCCV2-Lum-sgZBP#       10526 bp DNA     circular SYN 01-SEP-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10526)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 1, 2023 from SnapGene 7.0.0
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.1.0
FEATURES             Location/Qualifiers
     source          1..10526
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             25..150
                     /codon_start=1
                     /label=rble (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=97.6"
                     /note="/match_length=33.3"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="RDFDSTAAFYERLGFGIVFRDAGWMILQRGDLMLEFFAHPNL"
     polyA_signal    145..266
                     /label=rSV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
                     /note="color: #a6acb3"
     protein_bind    complement(339..355)
                     /label=rlac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
                     /note="color: #31849b; direction: LEFT"
     promoter        complement(363..393)
                     /label=rlac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     protein_bind    complement(408..429)
                     /label=rCAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
                     /note="color: #31849b; direction: LEFT"
     CDS             complement(442..534)
                     /codon_start=1
                     /label=rlacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="APNTQTASPRALADSLMQLARQVSRLESGQ"
     rep_origin      complement(717..1305)
                     /direction=LEFT
                     /label=rori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
                     /note="color: #ffff00"
     CDS             complement(1476..2336)
                     /codon_start=1
                     /label=rAmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2337..2441)
                     /label=rAmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    complement(2500..2552)
                     /label=rbom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=37.6"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3; direction: LEFT"
     enhancer        2707..3086
                     /label=rCMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
                     /note="color: #a6acb3"
     promoter        3087..3286
                     /label=rCMV promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=98.0"
                     /note="/other=promoter"
                     /note="color: #ffffff"
     LTR             3304..3484
                     /label=r5' LTR (truncated)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
                     /note="color: #ffe4c4"
     misc_feature    3531..3656
                     /label=rHIV-1 Psi
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3"
     CDS             3640..4003
                     /codon_start=1
                     /label=rHIV-1 gag (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.3"
                     /note="/match_length=24.2"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MGARASVLSGGELDRDGKKFG*GQGERKNIN*NI*YGQAGS*NDS
                     QLILAC*KHQKAVDKYWDSYNHPFRQDQKNLDHYIIQ*QPSIVCIKG*R*KTPRKL*TR
                     *RKSKTKVRPPHSKRPL"
     misc_feature    4149..4382
                     /label=rRRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3"
     CDS             4759..4859
                     /codon_start=1
                     /label=rRev (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.0"
                     /note="/match_length=28.8"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="THLPTPRGPDRPEGIEEEGGERDRDRSIRLVNG"
     misc_feature    4909..5026
                     /label=rcPPT/CTS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3"
     promoter        5077..5317
                     /label=rU6 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff"
     misc_feature    5327..5346
                     /label=sgZBP1#2
                     /note="color: #ff9900"
     misc_RNA        5348..5421
                     /label=rgRNA scaffold
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=97.4"
                     /note="/other=misc_RNA"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             5439..5457
                     /codon_start=1
                     /label=rsmall t antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.6"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="LGLERS"
     promoter        5483..5694
                     /label=rEF-1-alpha core promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.1"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff"
     CDS             5739..6766
                     /codon_start=1
                     /label=rCre (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.8"
                     /note="/match_length=99.6"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="CPIY*PYTKICLHYRSMQRVMRFART*WTCSGIARRFLSIPGKCF
                     SPFAGRGRHGAS*ITGNGFPQNLKMFAIIFYIFRRAVWQ*KLSSNIWAS*TCFIVGPGC
                     HDQVTAMLFHWLCGGSEKKTLMPVNVQNRL*RSNALISTRFVHSWKIAIAARIYVIWHF
                     WGLLITPCYV*PKLPGSGLKISHVLTVGEC*SILAERKRWLAPQV*RRHLAWG*LNWSS
                     DGFPYLV*LMIRITTCFAGSEKMVLPRHLPPASYQLAPWKGFLKQLID*FTALRMTLVR
                     DTWPGLDTVPVSEPREIWPALEFQYRRSCKLVAGPM*ILS*TISVTWIVKQGQWCACWK
                     MA"
     CDS             6824..7540
                     /codon_start=1
                     /label=rEGFP
                     /note="pLannotate"
                     /note="/database=fpbase"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             7556..8071
                     /codon_start=1
                     /label=rNluc
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     misc_feature    8087..8675
                     /label=rWPRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3"
     CDS             8671..8745
                     /codon_start=1
                     /label=rnef (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=80.0"
                     /note="/match_length=25.8"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="PASTLRPMTYKAAVDLSHFLKEKGG"
     LTR             8747..8980
                     /label=r3' LTR (Delta-U3)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
                     /note="color: #ffe4c4"
     polyA_signal    9012..9236
                     /label=rbGH poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
                     /note="color: #a6acb3"
     promoter        9240..9261
                     /label=rSV40 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=6.1"
                     /note="/other=promoter"
                     /note="color: #ffffff"
     rep_origin      9282..9710
                     /label=rf1 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
                     /note="color: #ffff00"
     promoter        9724..10053
                     /label=rSV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff"
     promoter        10101..10148
                     /label=rEM7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff"
     CDS             join(10167..10526,1..15)
                     /codon_start=1
                     /label=rBleoR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #ccffcc"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
ORIGIN
        1 gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg
       61 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
      121 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
      181 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
      241 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
      301 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
      361 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
      421 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
      481 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
      541 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      601 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      661 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      721 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      781 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      841 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      901 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      961 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     1021 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     1081 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     1141 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     1201 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     1261 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     1321 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     1381 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     1441 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     1501 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     1561 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     1621 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     1681 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     1741 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     1801 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     1861 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     1921 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     1981 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     2041 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     2101 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     2161 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     2221 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     2281 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     2341 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     2401 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     2461 ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
     2521 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
     2581 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
     2641 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
     2701 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
     2761 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     2821 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     2881 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     2941 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     3001 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     3061 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     3121 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     3181 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     3241 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
     3301 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     3361 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     3421 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     3481 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     3541 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     3601 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     3661 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     3721 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     3781 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     3841 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     3901 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     3961 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     4021 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     4081 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     4141 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     4201 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     4261 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     4321 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     4381 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     4441 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     4501 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     4561 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     4621 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     4681 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     4741 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     4801 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
     4861 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
     4921 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     4981 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     5041 ggacagcaga gatccagttt ggttaattaa ggtaccgagg gcctatttcc catgattcct
     5101 tcatatttgc atatacgata caaggctgtt agagagataa ttagaattaa tttgactgta
     5161 aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt
     5221 gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat
     5281 ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccgacct cttccttcac
     5341 ctcgcggttt agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa
     5401 aaagtggcac cgagtcggtg cttttttgaa ttcgctagct aggtcttgaa aggagtggga
     5461 attggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     5521 gggggagggg tcggcaattg atccggtgcc tagagaaggt ggcgcggggt aaactgggaa
     5581 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     5641 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggaccgg
     5701 ttctagagga tctaccatgc ccaagaagaa gaggaaggtg tccaatttac tgaccgtaca
     5761 ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca agaacctgat
     5821 ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc ttcagtccgt
     5881 ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc ccgcagaacc
     5941 tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag taaaaactat
     6001 ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc cacgaccaag
     6061 tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg ttgatgccgg
     6121 tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg ttcgttcact
     6181 catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg ggattgctta
     6241 taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata tctcacgtac
     6301 tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta gcaccgcagg
     6361 tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga tttccgtatc
     6421 tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa atggtgttgc
     6481 cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt ttgaagcaac
     6541 tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg cctggtctgg
     6601 acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt caataccgga
     6661 gatcatgcaa gctggtggct ggaccaatgt aaatattgtc atgaactata tccgtaacct
     6721 ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgatgcaa caaacttctc
     6781 tctgctgaaa caagccggag atgtcgaaga gaatcctgga ccgatggtga gcaagggcga
     6841 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     6901 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     6961 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     7021 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     7081 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     7141 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     7201 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     7261 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     7321 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     7381 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     7441 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     7501 cgccgccggg atcactctcg gcatggacga gctgtacaag gatatctccg gaggtatggt
     7561 cttcacactc gaagatttcg ttggggactg gcgacagaca gccggctaca acctggacca
     7621 agtccttgaa cagggaggtg tgtccagttt gtttcagaat ctcggggtgt ccgtaactcc
     7681 gatccaaagg attgtcctga gcggtgaaaa tgggctgaag atcgacatcc atgtcatcat
     7741 cccgtatgaa ggtctgagcg gcgaccaaat gggccagatc gaaaaaattt ttaaggtggt
     7801 gtaccctgtg gatgatcatc actttaaggt gatcctgcac tatggcacac tggtaatcga
     7861 cggggttacg ccgaacatga tcgactattt cggacggccg tatgaaggca tcgccgtgtt
     7921 cgacggcaaa aagatcactg taacagggac cctgtggaac ggcaacaaaa ttatcgacga
     7981 gcgcctgatc aaccccgacg gctccctgct gttccgagta accatcaacg gagtgaccgg
     8041 ctggcggctg tgcgaacgca ttctggcgta aacgcgttaa gtcgacaatc aacctctgga
     8101 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     8161 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     8221 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     8281 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     8341 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     8401 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     8461 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     8521 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     8581 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     8641 gacgagtcgg atctcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac
     8701 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     8761 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     8821 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     8881 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     8941 tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg
     9001 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     9061 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     9121 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     9181 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     9241 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     9301 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     9361 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     9421 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     9481 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     9541 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     9601 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     9661 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     9721 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     9781 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     9841 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     9901 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     9961 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
    10021 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
    10081 ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat
    10141 aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca
    10201 ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg
    10261 acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg
    10321 cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg
    10381 acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc
    10441 cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg
    10501 ccggcaactg cgtgcacttc gtggcc
//
