LOCUS       Simpson_B5H_16_p       10525 bp DNA     circular SYN 01-SEP-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 1, 2023 from SnapGene 7.0.0
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..10525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(257..685)
                     /direction=LEFT
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
                     /note="color: #ffff00"
     promoter        complement(706..727)
                     /label=SV40 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=6.1"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     polyA_signal    complement(731..955)
                     /label=bGH poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
                     /note="color: #a6acb3; direction: LEFT"
     LTR             complement(987..1220)
                     /label=3' LTR (Delta-U3)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
                     /note="color: #ffe4c4; direction: LEFT"
     CDS             complement(1222..1296)
                     /codon_start=1
                     /label=nef (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=80.0"
                     /note="/match_length=25.8"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="PASTLRPMTYKAAVDLSHFLKEKGG"
     misc_feature    complement(1292..1880)
                     /label=WPRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3; direction: LEFT"
     CDS             complement(1896..2411)
                     /codon_start=1
                     /label=Nluc
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             complement(2427..3143)
                     /codon_start=1
                     /label=EGFP
                     /note="pLannotate"
                     /note="/database=fpbase"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(3201..4228)
                     /codon_start=1
                     /label=Cre (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.8"
                     /note="/match_length=99.6"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="CPIY*PYTKICLHYRSMQRVMRFART*WTCSGIARRFLSIPGKCF
                     SPFAGRGRHGAS*ITGNGFPQNLKMFAIIFYIFRRAVWQ*KLSSNIWAS*TCFIVGPGC
                     HDQVTAMLFHWLCGGSEKKTLMPVNVQNRL*RSNALISTRFVHSWKIAIAARIYVIWHF
                     WGLLITPCYV*PKLPGSGLKISHVLTVGEC*SILAERKRWLAPQV*RRHLAWG*LNWSS
                     DGFPYLV*LMIRITTCFAGSEKMVLPRHLPPASYQLAPWKGFLKQLID*FTALRMTLVR
                     DTWPGLDTVPVSEPREIWPALEFQYRRSCKLVAGPM*ILS*TISVTWIVKQGQWCACWK
                     MA"
     promoter        complement(4273..4484)
                     /label=EF-1-alpha core promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.1"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     CDS             complement(4510..4528)
                     /codon_start=1
                     /label=small t antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.6"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="LGLERS"
     misc_RNA        complement(4546..4620)
                     /label=gRNA scaffold
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=98.7"
                     /note="/other=misc_RNA"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    4621..4639
                     /label=sgMLKL
                     /note="color: #ff9900"
     promoter        complement(4649..4889)
                     /label=U6 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    complement(4940..5057)
                     /label=cPPT/CTS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3; direction: LEFT"
     CDS             complement(5107..5207)
                     /codon_start=1
                     /label=Rev (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.0"
                     /note="/match_length=28.8"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="THLPTPRGPDRPEGIEEEGGERDRDRSIRLVNG"
     misc_feature    complement(5584..5817)
                     /label=RRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3; direction: LEFT"
     CDS             complement(5963..6326)
                     /codon_start=1
                     /label=HIV-1 gag (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.3"
                     /note="/match_length=24.2"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MGARASVLSGGELDRDGKKFG*GQGERKNIN*NI*YGQAGS*NDS
                     QLILAC*KHQKAVDKYWDSYNHPFRQDQKNLDHYIIQ*QPSIVCIKG*R*KTPRKL*TR
                     *RKSKTKVRPPHSKRPL"
     misc_feature    complement(6310..6435)
                     /label=HIV-1 Psi
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3; direction: LEFT"
     LTR             complement(6482..6662)
                     /label=5' LTR (truncated)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
                     /note="color: #ffe4c4; direction: LEFT"
     promoter        complement(6680..6879)
                     /label=CMV promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=98.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     enhancer        complement(6880..7259)
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    7414..7466
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=37.6"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3"
     promoter        7525..7629
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff"
     CDS             7630..8490
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8661..9249
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
                     /note="color: #ffff00"
     CDS             9432..9524
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="APNTQTASPRALADSLMQLARQVSRLESGQ"
     protein_bind    9537..9558
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
                     /note="color: #31849b"
     promoter        9573..9603
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff"
     protein_bind    9611..9627
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
                     /note="color: #31849b"
     polyA_signal    complement(9700..9821)
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
                     /note="color: #a6acb3; direction: LEFT"
     CDS             complement(9816..9941)
                     /codon_start=1
                     /label=ble (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=97.6"
                     /note="/match_length=33.3"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="RDFDSTAAFYERLGFGIVFRDAGWMILQRGDLMLEFFAHPNL"
     CDS             complement(9951..10325)
                     /codon_start=1
                     /label=BleoR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #ccffcc"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(10344..10391)
                     /label=EM7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     promoter        complement(join(10439..10525,1..243))
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
       61 gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat
      121 acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg
      181 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca
      241 cacattccac agaattaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
      301 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
      361 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
      421 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
      481 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
      541 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
      601 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
      661 cttaatgcgc cgctacaggg cgcgtgggga taccccctag agccccagct ggttctttcc
      721 gcctcagaag ccatagagcc caccgcatcc ccagcatgcc tgctattgtc ttcccaatcc
      781 tcccccttgc tgtcctgccc caccccaccc cccagaatag aatgacacct actcagacaa
      841 tgcgatgcaa tttcctcatt ttattaggaa aggacagtgg gagtggcacc ttccagggtc
      901 aaggaaggca cgggggaggg gcaaacaaca gatggctggc aactagaagg cacagtcgag
      961 gctgatcagc gggtttaaac gggccctgct agagattttc cacactgact aaaagggtct
     1021 gagggatctc tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca
     1081 aggcaagctt tattgaggct taagcagtgg gttccctagt tagccagaga gctcccaggc
     1141 tcagatctgg tctaaccaga gagacccagt acaagcaaaa agcagatctt gtcttcgttg
     1201 ggagtgaatt agcccttcca gtcccccctt ttcttttaaa aagtggctaa gatctacagc
     1261 tgccttgtaa gtcattggtc ttaaagtcga cgcggggagg cggcccaaag ggagatccga
     1321 ctcgtctgag ggcgaaggcg aagacgcgga agaggccgca gagccggcag caggccgcgg
     1381 gaaggaaggt ccgctggatt gagggccgaa gggacgtagc agaaggacgt cccgcgcaga
     1441 atccaggtgg caacacaggc gagcagccaa ggaaaggacg atgatttccc cgacaacacc
     1501 acggaattgt cagtgcccaa cagccgagcc cctgtccagc agcgggcaag gcaggcggcg
     1561 atgagttccg ccgtggcaat agggaggggg aaagcgaaag tcccggaaag gagctgacag
     1621 gtggtggcaa tgccccaacc agtgggggtt gcgtcagcaa acacagtgca caccacgcca
     1681 cgttgcctga caacgggcca caactcctca taaagagaca gcaaccagga tttatacaag
     1741 gaggagaaaa tgaaagccat acgggaagca atagcatgat acaaaggcat taaagcagcg
     1801 tatccacata gcgtaaaagg agcaacatag ttaagaatac cagtcaatct ttcacaaatt
     1861 ttgtaatcca gaggttgatt gtcgacttaa cgcgtttacg ccagaatgcg ttcgcacagc
     1921 cgccagccgg tcactccgtt gatggttact cggaacagca gggagccgtc ggggttgatc
     1981 aggcgctcgt cgataatttt gttgccgttc cacagggtcc ctgttacagt gatctttttg
     2041 ccgtcgaaca cggcgatgcc ttcatacggc cgtccgaaat agtcgatcat gttcggcgta
     2101 accccgtcga ttaccagtgt gccatagtgc aggatcacct taaagtgatg atcatccaca
     2161 gggtacacca ccttaaaaat tttttcgatc tggcccattt ggtcgccgct cagaccttca
     2221 tacgggatga tgacatggat gtcgatcttc agcccatttt caccgctcag gacaatcctt
     2281 tggatcggag ttacggacac cccgagattc tgaaacaaac tggacacacc tccctgttca
     2341 aggacttggt ccaggttgta gccggctgtc tgtcgccagt ccccaacgaa atcttcgagt
     2401 gtgaagacca tacctccgga gatatccttg tacagctcgt ccatgccgag agtgatcccg
     2461 gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc
     2521 agggcggact gggtgctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg
     2581 ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg
     2641 atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg
     2701 ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg
     2761 cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc
     2821 ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg
     2881 gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa gcactgcacg
     2941 ccgtaggtca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag
     3001 atgaacttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg
     3061 aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac cccggtgaac
     3121 agctcctcgc ccttgctcac catcggtcca ggattctctt cgacatctcc ggcttgtttc
     3181 agcagagaga agtttgttgc atcgccatct tccagcaggc gcaccattgc ccctgtttca
     3241 ctatccaggt tacggatata gttcatgaca atatttacat tggtccagcc accagcttgc
     3301 atgatctccg gtattgaaac tccagcgcgg gccatatctc gcgcggctcc gacacgggca
     3361 ctgtgtccag accaggccag gtatctctga ccagagtcat ccttagcgcc gtaaatcaat
     3421 cgatgagttg cttcaaaaat cccttccagg gcgcgagttg atagctggct ggtggcagat
     3481 ggcgcggcaa caccattttt tctgacccgg caaaacaggt agttattcgg atcatcagct
     3541 acaccagata cggaaatcca tcgctcgacc agtttagtta cccccaggct aagtgccttc
     3601 tctacacctg cggtgctaac cagcgttttc gttctgccaa tatggattaa cattctccca
     3661 ccgtcagtac gtgagatatc tttaaccctg atcctggcaa tttcggctat acgtaacagg
     3721 gtgttataag caatccccag aaatgccaga ttacgtatat cctggcagcg atcgctattt
     3781 tccatgagtg aacgaacctg gtcgaaatca gtgcgttcga acgctagagc ctgttttgca
     3841 cgttcaccgg catcaacgtt ttcttttcgg atccgccgca taaccagtga aacagcattg
     3901 ctgtcacttg gtcgtggcag cccggaccga cgatgaagca tgtttagctg gcccaaatgt
     3961 tgctggatag tttttactgc cagaccgcgc gcctgaagat atagaagata atcgcgaaca
     4021 tcttcaggtt ctgcgggaaa ccatttccgg ttattcaact tgcaccatgc cgcccacgac
     4081 cggcaaacgg actgaagcat tttccaggta tgctcagaaa acgcctggcg atccctgaac
     4141 atgtccatca ggttcttgcg aacctcatca ctcgttgcat cgaccggtaa tgcaggcaaa
     4201 ttttggtgta cggtcagtaa attggacacc ttcctcttct tcttgggcat ggtagatcct
     4261 ctagaaccgg tcctgtgttc tggcggcaaa cccgttgcga aaaagaacgt tcacggcgac
     4321 tactgcactt atatacggtt ctcccccacc ctcgggaaaa aggcggagcc agtacacgac
     4381 atcactttcc cagtttaccc cgcgccacct tctctaggca ccggatcaat tgccgacccc
     4441 tccccccaac ttctcgggga ctgtgggcga tgtgcgctct gcccactgac gggcaccgga
     4501 gccaattccc actcctttca agacctagct agcgaattca aaaaagcacc gactcggtgc
     4561 cactttttca agttgataac ggactagcct tattttaact tgctatttct agctctaaaa
     4621 gcgtctagga aaccgtgtgc ggtgtttcgt cctttccaca agatatataa agccaagaaa
     4681 tcgaaatact ttcaagttac ggtaagcata tgatagtcca ttttaaaaca taattttaaa
     4741 actgcaaact acccaagaaa ttattacttt ctacgtcacg tattttgtac taatatcttt
     4801 gtgtttacag tcaaattaat tctaattatc tctctaacag ccttgtatcg tatatgcaaa
     4861 tatgaaggaa tcatgggaaa taggccctcg gtaccttaat taaccaaact ggatctctgc
     4921 tgtccctgta ataaacccga aaattttgaa tttttgtaat ttgtttttgt aattctttag
     4981 tttgtatgtc tgttgctatt atgtctacta ttctttcccc tgcactgtac cccccaatcc
     5041 ccccttttct tttaaaattg tggatgaata ctgccatttg tctgcagaat tggcgcacgc
     5101 agtgccgatc cgttcactaa tcgaatggat ctgtctctgt ctctctctcc accttcttct
     5161 tctattcctt cgggcctgtc gggtcccctc ggggttggga ggtgggtctg aaacgataat
     5221 ggtgaatatc cctgcctaac tctattcact atagaaagta cagcaaaaac tattcttaaa
     5281 cctaccaagc ctcctactat cattatgaat aattttatat accacagcca atttgttatg
     5341 ttaaaccaat tccacaaact tgcccattta tctaattcca ataattcttg ttcattcttt
     5401 tcttgctggt tttgcgattc ttcaattaag gagtgtatta agcttgtgta attgttaatt
     5461 tctctgtccc actccatcca ggtcgtgtga ttccaaatct gttccagaga tttattactc
     5521 caactagcat tccaaggcac agcagtggtg caaatgagtt ttccagagca accccaaatc
     5581 cccaggagct gttgatcctt taggtatctt tccacagcca ggattcttgc ctggagctgc
     5641 ttgatgcccc agactgtgag ttgcaacaga tgctgttgcg cctcaatagc cctcagcaaa
     5701 ttgttctgct gctgcactat accagacaat aattgtctgg cctgtaccgt cagcgtcatt
     5761 gacgctgcgc ccatagtgct tcctgctgct cccaagaacc caaggaacaa agctcctatt
     5821 cccactgctc ttttttctct ctgcaccact cttctctttg ccttggtggg tgctactcct
     5881 aatggttcaa tttttactac tttatattta tataattcac ttctccaatt gtccctcata
     5941 tctcctcctc caggtctgaa gatcagcggc cgcttgctgt gcggtggtct tacttttgtt
     6001 ttgctcttcc tctatcttgt ctaaagcttc cttggtgtct tttatctcta tcctttgatg
     6061 cacacaatag agggttgcta ctgtattata taatgatcta agttcttctg atcctgtctg
     6121 aagggatggt tgtagctgtc ccagtatttg tctacagcct tctgatgttt ctaacaggcc
     6181 aggattaact gcgaatcgtt ctagctccct gcttgcccat actatatgtt ttaatttata
     6241 ttttttcttt ccccctggcc ttaaccgaat tttttcccat cgcgatctaa ttctcccccg
     6301 cttaatactg acgctctcgc acccatctct ctccttctag cctccgctag tcaaaatttt
     6361 tggcgtactc accagtcgcc gcccctcgcc tcttgccgtg cgcgcttcag caagccgagt
     6421 cctgcgtcga gagagctcct ctggtttccc tttcgctttc aagtccctgt tcgggcgcca
     6481 ctgctagaga ttttccacac tgactaaaag ggtctgaggg atctctagtt accagagtca
     6541 cacaacagac gggcacacac tacttgaagc actcaaggca agctttattg aggcttaagc
     6601 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
     6661 ccagtacagg caaaacgcgc tgcttatata gacctcccac cgtacacgcc taccgcccat
     6721 ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca
     6781 aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg
     6841 ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa
     6901 tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca
     6961 ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg
     7021 atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt
     7081 ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt
     7141 gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg
     7201 gctatgaact aatgaccccg taattgatta ctattaataa ctagtcaata atcaatgtca
     7261 acgcgtatat ctggcccgta catcgcgaag cagcgcaaaa cgcctaaccc taagcagatt
     7321 cttcatgcaa ttgtcggtca agccttgcct tgttgtagct taaattttgc tcgcgcacta
     7381 ctcagcgacc tccaacacac aagcagggag cagatactgg cttaactatg cggcatcaga
     7441 gcagattgta ctgagagtgc accatagggg atcgggagat ctcccgatcc gtcgacgtca
     7501 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     7561 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     7621 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     7681 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     7741 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     7801 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     7861 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     7921 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     7981 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     8041 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     8101 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     8161 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     8221 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     8281 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     8341 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     8401 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     8461 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     8521 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     8581 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     8641 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     8701 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     8761 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     8821 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     8881 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     8941 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     9001 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     9061 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     9121 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     9181 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     9241 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     9301 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     9361 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     9421 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     9481 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     9541 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     9601 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     9661 gccaagctct agctagaggt cgacggtata cagacatgat aagatacatt gatgagtttg
     9721 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     9781 ttgctttatt tgtaaccatt ataagctgca ataaacaagt tggggtgggc gaagaactcc
     9841 agcatgagat ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag
     9901 cccaaccttt catagaaggc ggcggtggaa tcgaaatctc gtagcacgtg tcagtcctgc
     9961 tcctcggcca cgaagtgcac gcagttgccg gccgggtcgc gcagggcgaa ctcccgcccc
    10021 cacggctgct cgccgatctc ggtcatggcc ggcccggagg cgtcccggaa gttcgtggac
    10081 acgacctccg accactcggc gtacagctcg tccaggccgc gcacccacac ccaggccagg
    10141 gtgttgtccg gcaccacctg gtcctggacc gcgctgatga acagggtcac gtcgtcccgg
    10201 accacaccgg cgaagtcgtc ctccacgaag tcccgggaga acccgagccg gtcggtccag
    10261 aactcgaccg ctccggcgac gtcgcgcgcg gtgagcaccg gaacggcact ggtcaacttg
    10321 gccatggttt agttcctcac cttgtcgtat tatactatgc cgatatacta tgccgatgat
    10381 taattgtcaa cacgtgctga tcagatccga aaatggatat acaagctccc gggagctttt
    10441 tgcaaaagcc taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggcag
    10501 aggcggcctc ggcctctgca taaat
//
