LOCUS       H129N_3                 7896 bp    DNA     circular SYN 18-NOV-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     LTR             complement(7178..7647)
                     /note="pLannotate"
                     /label="LTR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
     LTR             complement(1796..2265)
                     /note="pLannotate"
                     /label="LTR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
     CDS             complement(6297..6713)
                     /note="pLannotate"
                     /label="gag (truncated)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(3104..3314)
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        7678..7782
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(5367..6256)
                     /note="pLannotate"
                     /label="HaloTag® (fragment)"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="99.9"
                     /fragment="True"
                     /other="CDS"
     CDS             join(7783..7896,1..747)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(3461..5302)
                     /note="pLannotate"
                     /label="MRE11 (fragment)"
                     /database="swissprot"
                     /identity="97.2"
                     /match_length="86.7"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(2486..3086)
                     /note="pLannotate"
                     /label="PuroR (fragment)"
                     /database="snapgene"
                     /identity="99.3"
                     /match_length="100.2"
                     /fragment="True"
                     /other="CDS"
     rep_origin      907..1495
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     misc_feature    complement(6780..7054)
                     /note="pLannotate"
                     /label="MESV Ψ (fragment)"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="80.4"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(5333..5353)
                     /note="pLannotate"
                     /label="TEV site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     LTR             complement(2266..2387)
                     /note="pLannotate"
                     /label="3' LTR (ΔU3) (fragment)"
                     /database="snapgene"
                     /identity="97.6"
                     /match_length="28.9"
                     /fragment="True"
                     /other="LTR"
     promoter        complement(3341..3413)
                     /note="pLannotate"
                     /label="SV40 promoter (fragment)"
                     /database="snapgene"
                     /identity="98.6"
                     /match_length="24.5"
                     /fragment="True"
                     /other="promoter"
     misc_feature    complement(5304..5326)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="40.4"
                     /fragment="True"
                     /other="misc_feature"
     misc_feature    complement(3437..3455)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="35.8"
                     /fragment="True"
                     /other="misc_feature"
     CDS             1678..1730
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="4.9"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
       61 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
      121 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
      181 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
      241 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
      301 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
      361 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
      421 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
      481 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
      541 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
      601 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
      661 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
      721 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
      781 attgatttgc ggccgcaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
      841 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
      901 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
      961 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1021 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     1081 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1141 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1201 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     1261 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     1321 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     1381 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     1441 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     1501 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     1561 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     1621 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     1681 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca actatggcca
     1741 tttaatgtaa atacttaaga aaaaaaacca aattaatttt gatacatgct gcatgtgaaa
     1801 gacccccgct gacgggtagt caatcactca gaggagaccc tcccaaggaa cagcgagacc
     1861 acaagtcgga tgcaactgca agagggttta ttggatacac gggtacccgg gcgactcagt
     1921 caatcggagg actggcgccc cgagtgaggg gttgtgggct cttttattga gctcggggag
     1981 cagaagcgcg cgaacagaag cgagaagcga actgattggt tagttcaaat aaggcacagg
     2041 gtcatttcag gtccttgggg caccctggaa acatctgatg gttctctaga aactgctgag
     2101 ggctggaccg catctgggga ccatctgttc ttggccctga gccggggcag gaactgctta
     2161 ccacagatat cctgtttggc ccatattcag ctgttccatc tgttcttggc cctgagccgg
     2221 ggcaggaact gcttaccaca gatatcctgt ttggcccata ttcagctgtt ccatctgttc
     2281 ctgaccttga tctgaacttc tctattctca gttatgtatt tttccatgcc ttgcaaaatg
     2341 gcgttactta agctagcttg ccaaacctac aggtggggtc tttcattccc ccctttttct
     2401 ggagactaaa taaaatcttt tattttatcg atgcatgggg tcgtgcgctc ctttcggtcg
     2461 ggcgctgcgg gtcgtggggc gggcgtcagg caccgggctt gcgggtcatg caccaggtgc
     2521 gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa gccgagccgc tcgtagaagg
     2581 ggaggttgcg gggcgcggag gtctccgagg aaggcgggca ccccggcgcg ctcggccgcc
     2641 tccactccgg ggagcacgac ggcgctgccc agacccttgc cctggtggtc gggcgagacg
     2701 ccgacggtgg ccgggaacca cgcgggctcc ttgggccggt gcggcgccag gaggccttcc
     2761 atctgttgct gcgcggccag ccgggaaccg ctcaactcgg ccatgcgcgg gccgatctcg
     2821 gcgaacaccg cccccgcttc gacgctctcc ggcgtggtcc agaccgccac cgcggcgccg
     2881 tcgtccgcga cccacacctt gccgatgtcg agcccgacgc gcgtgaggaa gagttcttgc
     2941 agctcggtga cccgctcgat gtggcggtcc ggatcgacgg tgtggcgcgt ggcggggtag
     3001 tcggcgaacg cggcggcgag ggtgcgtacg gccccgggga cgtcgtcgcg ggtggcgagg
     3061 cgcaccgtgg gcttgtactc ggtcatggta agctttttgc aaaagcctag gcctccaaaa
     3121 aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa
     3181 ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc
     3241 gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc
     3301 atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga
     3361 tgcatgcttt gcatacttct gcctgctggg gagcactggg gactttccac accctaactg
     3421 acacacattc cacagggtcg actgcagaat tcgaacctta ggaaggctgc tgtcttgtag
     3481 atttaaaggc atctataata gacatatttc tagatgctga cacagcagtc tttgagttcc
     3541 tgctacgggt agaagtctcc agaccagtgt ctgctcttcc tctttgagac cctcctctcg
     3601 atgctgaatt ctgccctctt ccacctcttc gacctcttcc tcggcctctt cctttgttgg
     3661 ttgctgctga gatgctatca tcagagtcat tagccatctg ttctgctaaa tctatactca
     3721 taaggtcatc agcactaaag gcagaagcag actcctctga ctgagatctg agtgctctgg
     3781 ccctggtcat agcctcacgg acttcatcat cttcttcatt agtatttttt tgtctggttt
     3841 ctctgaaacg acgtacctcc tcatcgattt tgtcttcgag ggcatcaatg tgacgttctt
     3901 taagaaatcg ctgtgttttt tccaactggt atttcactaa ttcctcaatg gcatctttct
     3961 cctccttgtc cacaaattct tgtactgctt cacccatccc tctttctgtt agcagtgaga
     4021 gctgcacatt cttctctgcg gtttgaaagt actgttttac aagatcttct acccttaaag
     4081 ttgttccttc ttgttctcta tgcctgaaaa aatggataat gtcttttgga ttagctaccc
     4141 gatccacaaa tttctggcta aagcgaagaa cactgaaagg ttcaaaacct ccactatagt
     4201 ccactcgcag tcgtacaaga ggcttctctg gctggtgaga attacccaga cgttcccgtt
     4261 cagcattttc aagcatttct tcaatcttct ccaaacagaa gctttgtatg gcttgggtta
     4321 ctttaggatt atctgggtta aaaatgtctg gatgattagc tagaacaata tcctccatga
     4381 aaaactgccg cactgtgtga agaggaattt tatgcatatt catcttcctc cctttaatac
     4441 gcagcaaacc aacatgtttc tttacagctt ctcctgggga aagagaagta accactgagc
     4501 ttccaggttg tgagatataa aacagctgtt gttcattttt ggttggagct attttacact
     4561 catgttcatg gccccagata acaagatcaa tgaagtcatc caaaaattgt tctggaatga
     4621 agttagtact tccatgttta ctcctgttct gatgaatcac aaataagtta aaccaagagt
     4681 tctcatcttc ctttggtctc aacattgtta cttttttatt gacaaacatt cgatagagcc
     4741 tttcatctgg aatggatcct aaaccatata gcgcaatctt tgtgcttcct ttttgaagca
     4801 aaaccggact aatgtctatc ttctccacag acattgaacg tccaaagtga tttacaaatc
     4861 cagcacaact taaaatgtcc aaggcacaaa gtgcatctgc ccctgtggga tcgtcatgat
     4921 tgccatgaat actaaacact ggaattgaaa tgttgaggtt gccatcttga tagttcaccc
     4981 atggaaactt actaaaacca aagttgactg actgatcact gagaatttca aactggacag
     5041 gccgatcacc catacaatat tttcttaata actcgaggca ggtatgtaat gttttccttg
     5101 agggcttatt ttcatgaaaa agatcaccac ctaacaaaat aaaatccact tcattttcct
     5161 gggcaagtct taaaatttca tcgagtgtta caaacgtatc atttcctctg actgcatctt
     5221 tctccataaa tccaagatga atatctgttg caactaatat tttaaatgtg ttttcatcat
     5281 caagtgcatc tgcagtactc atcttcgaag cttgagctcg agatctgtta tcgctctgaa
     5341 agtacagatc ctcagtggtt ggctcaccgg aaatctccag agtagacagc cagcgcgcga
     5401 tctcgctgcc gatcaggtcc gggttgtctt cttgcagcag attcagaccc gggccgatgt
     5461 ccacagcctt gcagttaggc aggcttttgg ccaggcgagc ggcttcggcc ggtgggatca
     5521 gaacgcctgg ggtgccccag aacagcagct tcgggacagg ggactggtgc agccagtcca
     5581 tgtattcttc gaccagcgcg acgatgttcg ctggctcacc ggcgattggc agctcgtttg
     5641 ggaagcgcca cagtggctcg cggtcaacag gattcaggaa cggctcgcgg taatggtcca
     5701 tctcgacttc agtcagcggg cggacgacac ccatcggcag cgtaccctcg ataaaaacgt
     5761 tctgatcgat gatcagcttg cggccgacgt cggtggtgcg gaaggcctgg aaggtctcgc
     5821 gggcaaattc tggccattcg tcccaggtcg ggatagggcg gatgaactcc ataaatgcaa
     5881 tacctttgac gcgctctgga ttgcgcttgg cccagtggaa acccagagcg gagccccagt
     5941 cgtgaatgac caggacgacc tcttccagac ccagggcttc gatgaaggca tccatgaagc
     6001 ggacgtggtc gtcgaagaaa taacccaggt ctggtttgtc ggatttgccc ataccgatca
     6061 ggtctggagc aatgcagcga tgggtcggtg caacatgcgg gatgatgttg cgccacacgt
     6121 aggaggaggt cgggttaccg tgcaggaaca gcacaggggt gccatcgcgc ggaccaacat
     6181 cgacgtagtg catgcgctcg cccaggactt ccacataatg ggggtcgaat ggaaagccag
     6241 taccgatttc tgccatggtg gcgaccggta gcgtaccacc acactgggat ccggccggcg
     6301 cctagagaag gagtgagggc tggataaagg gaggatcgag gcggggtcga acgaggaggt
     6361 tcaaggggga gagacggggc ggatggagga agaggaggcg gaggcttagg gtgtacaaag
     6421 ggcttgaccc agggaggggg gtcaaaagcc aaggcttccc aggtcacgat gtaggggacc
     6481 tggtctgggt gtccatgcgg gccaggtgaa aagaccttga tcttaacctg ggtgatgagg
     6541 tctcggttaa aggtgccgtc tcgcggccat ccgacgttaa aggttggcca ttctgcagag
     6601 cagaaggtaa cccaacgtct cttcttgaca tctaccgact ggttgtgagc gatccgctcg
     6661 acatctttcc agtgacctaa ggtcaaactt aagggagtgg taacagtctg gccctaattt
     6721 tcagacaaat acagaaacac agtcagacag agacaacaca gaacgatgct gcagcgctgc
     6781 agcagacaag acgcgcggct tcggttccaa accgaaagca aaaattcaga cggaggcggg
     6841 aactgtttta ggttctcgtc tcctaccaga accacatatc ctgacggggt cggattccac
     6901 atcgactccc ttcctcaggt cgggccacaa aaacggcccc caaagtccct gggacgtctc
     6961 ccagggttgc ggccgggtgt tcagaactcg tcagttccac cacgggtccg ccagatacag
     7021 agctagttag ctaactagta ccgacgcagg cgcataaaat cagtcataga cactagacaa
     7081 tcggacagac acagataagt tgctggccag cttgcctccc ggtggtgggt cggtggtccc
     7141 tgggcagggg tctcccgatc ccggacgagc ccccaaatga aagacccccg ctgacgggta
     7201 gtcaatcact cagaggagac cctcccaagg aacagcgaga ccacaagtcg gatgcaactg
     7261 caagagggtt tattggatac acgggtaccc gggcgactca gtcaatcgga ggactggcgc
     7321 cccgagtgag gggttgtggg ctcttttatt gagctcgggg agcagaagcg cgcgaacaga
     7381 agcgagaagc gaactgattg gttagttcaa ataaggcaca gggtcatttc aggtccttgg
     7441 ggcaccctgg aaacatctga tggttctcta gaaactgctg agggctggac cgcatctggg
     7501 gaccatctgt tcttggccct gagccggggc aggaactgct taccacagat atcctgtttg
     7561 gcccatattc agctgttcca tctgttcttg gccctgagcc ggggcaggaa ctgcttacca
     7621 cagatatcct gtttggccca tattcaggct gcaggtggca cttttcgggg aaatgtgcgc
     7681 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     7741 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     7801 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     7861 acgctggtga aagtaaaaga tgctgaagat cagttg
//
