LOCUS       CMV-SynCas.v10-bGH-AmpR 7169 bp ds-DNA     circular     13-SEP-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        complement(766..870)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     enhancer        1140..1519
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     promoter        1520..1723
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    1765..2478
                     /label="Cas7.1"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1765..3639
                     /label="DiCas7-11 Frag"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1765..5565
                     /label="SynCas.v10"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             1765..5565
                     /label="Translation 1765-5565"
                     /translation="MTTTMKISIEFLEPFRMTKWQESTRRNKNNKEFVRGQAFARWHRNKKDNTKGRPYITGTLLRSAVIRSAENLLTLSDGKISEKTCCPGKFDTEDKDRLLQLRQRSTLRWTDKNPCPDNAETYCPFCELLGRSGNDGKKAEKKDWRFRIHFGNLSLPGKPDFDGPKAIGSQRVLNRVDFKSGKAHDFFKAYEVDHTRFPRFEGEITIDNKVSAEARKLLCDSLKFTDRLCGALCVIRFDEYTPAADSGKQTENVQAEPNANLAEKTAEQIISILDDNKKTEYTRLLADAIRSLRRSSKLVAGLPKDHDGKDDHYLWDIGKKKKDENSVTIRQILTTSADTKELKNAGKWREFCEKLGEALYLKSKDMSGGLKITRRILGDAEFHGKPDRLEKSRSVSIGSVLKETVVCGELVAKTPFFFGAIDEDAKQTDLQVLLTPDNKYRLPRSAVRGILRRDLQTYFDSPCNAELGGRPCMCKTCRIMRGITVMDARSEYNAPPEIRHRTRINPFTGTVAEGALFNMEVAPEGIVFPFQLRYRGSEDGLPDALKTVLKWWAEGQAFMSGAASTGKGRFRMENAKYETLDLSDENQRNDYLKNWGWRDEKGLEELKKRLNSGLPEPGNYRDPKWTKVGYEIKIQSPLLSNDPIAALFDTANHDAVAYKKRVWENGRIIERHTFKGESIRGVLRTALGREYGLFELKHEDCPCSLCTIFGNEHEAGKIRFEDLEIEDGVAEKHFDHVAIDRFTGGAVDKKKFDDFSLAGSPGKPIKLKGCFWLRADIFSDEKKDEKKKIINALCDVRDGLYPLGGKGGVGYGWVKELRITDGPDDFKLPEPKCKEIPPDGTPSDYPFPQLKPLQTEKDKVYSPHYFLKPAEKKVDRNWEKRLIGHERFDKDLLTGKIECTLKTLSPLIIPDSEVVTENGNGHKSYQFFRLNNEIIIPGSEIRGMISSVYEALTNSCFRVVDDIIPVVISRSVSPYQIAQKLPSPDLRPCEREILEDSEIGSLDKCSEKRLFRLHEDGLCPSCRLFGTTHYKGRVRFGFAKHEGGEKWLMDDKTKDEYLTLPLLERPRPTWSMPSDEDDVPGRKFYVHHNGWEKVKQDSLDNKTSKTENNRSVEPLDKDNLFKFDVFFENLEPWELGLLLYSLELEEGLAHKFGMAKAFGFGSTKIDADKILLREAPNEWQDATNRKDDMLRKGFGELTSWFDKDWDKVEHIDGLRSLLQIPEALSRAEVRYPDLTEDDPEKPPGYVQLSDKKEGRKYLIRPWQPWY*"
     misc_feature    2479..2541
                     /label="Linker 1"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2542..2859
                     /label="Cas11"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2860..2967
                     /label="Linker 2"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2968..3510
                     /label="Cas7.2"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3511..3639
                     /label="Linker 3"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    3640..4227
                     /label="Cas7.3"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3640..5565
                     /label="Cas7-1 933AA"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    4228..4443
                     /label="Linker"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    4444..4671
                     /label="Cas7.4-1"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    4672..4689
                     /label="INS"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    4690..5283
                     /label="Cas7.4-2"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    5284..5562
                     /label="CTE"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             5572..5589
                     /label="6xHis"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     polyA_signal    5618..5842
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     complement(5913..5929)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     protein_bind    5937..5953
                     /label="lac operator"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        complement(5961..5991)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     protein_bind    6006..6027
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     rep_origin      complement(6315..6903)
                     /label="ori"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             complement(7074..765)
                     /label="AmpR"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
ORIGIN
        1 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
       61 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
      121 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
      181 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
      241 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
      301 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
      361 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
      421 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
      481 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
      541 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
      601 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
      661 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
      721 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
      781 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
      841 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
      901 cgacggatcg ggagatcgat ctcccgatcc cctagggtcg actctcagta caatctgctc
      961 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
     1021 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
     1081 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
     1141 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     1201 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     1261 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     1321 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     1381 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     1441 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     1501 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     1561 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     1621 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     1681 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca
     1741 gatccgctag agatccgcgg ccgcatgacg actactatga agatttcaat tgaattcctc
     1801 gagccgtttc ggatgaccaa atggcaggaa agcactaggc gaaacaagaa caacaaggaa
     1861 tttgttcgcg gccaagcttt tgcaagatgg catagaaata agaaggataa cacaaaaggg
     1921 cggccttaca taactgggac attgctgcgg tccgcagtaa ttagaagcgc ggaaaacctg
     1981 ctgacattga gtgatgggaa aattagcgaa aagacatgtt gtcctgggaa atttgatacg
     2041 gaagataaag acaggttgct gcaattgagg cagcggtcca cccttcgttg gaccgataag
     2101 aacccctgtc cagataacgc tgaaacttac tgcccgtttt gcgaacttct cgggagatcc
     2161 ggtaacgatg gaaagaaggc tgagaagaag gattggcgct ttcgtattca ttttggcaat
     2221 ctttcccttc cagggaagcc cgatttcgac gggccaaaag ctataggcag ccaacgggta
     2281 cttaacaggg tcgatttcaa atccggtaag gcccatgact tctttaaggc ttacgaagtc
     2341 gaccatacta ggtttccccg cttcgaaggg gagattacca tagataataa agtcagcgct
     2401 gaagccagga aactgctctg tgattctctc aagtttacgg atcggctgtg tggagctctg
     2461 tgcgtaatca ggttcgatga atatacacca gcagccgata gtgggaaaca aaccgaaaat
     2521 gtccaagcag aaccgaatgc taatctcgct gaaaagaccg cagagcaaat tattagcatt
     2581 ctggacgata ataagaaaac cgaatatacc cgcctgcttg ctgatgctat tcgttcactc
     2641 cgccgctcta gtaaacttgt cgctggcttg ccaaaggatc atgacgggaa ggatgaccat
     2701 tacctctggg atatcggcaa gaagaagaaa gacgaaaact ctgtcactat taggcaaatc
     2761 cttacgacct cagcagacac caaggaactc aagaatgccg gaaaatggag agaattctgc
     2821 gagaagctgg gtgaagcgct gtacctcaag agtaaagaca tgagtggcgg cctgaaaatt
     2881 actcggagaa tactgggcga tgctgaattc catggaaagc ccgatcggct tgaaaagagc
     2941 cggtctgtgt caatcggatc tgtgttgaaa gaaactgtgg tatgcggcga actggtcgct
     3001 aaaacgccct tcttcttcgg agcgatagac gaagatgcaa aacaaaccga cctgcaagta
     3061 ctcctcactc ccgataacaa gtatagactg ccaagaagcg ccgtgcgagg tatactccgt
     3121 cgagatcttc aaacctattt tgatagccca tgcaatgctg agttgggtgg acggccatgc
     3181 atgtgtaaga cgtgtaggat tatgagaggg atcacggtga tggatgcgcg cagtgagtac
     3241 aatgccccgc cagaaataag gcatcgtacc cgcattaatc ccttcacagg cacggttgcg
     3301 gaaggtgccc tgtttaatat ggaagtagcc cccgagggga ttgtctttcc atttcaactc
     3361 cggtaccggg gctctgaaga tgggctgccc gatgcactga aaacggtgtt gaaatggtgg
     3421 gctgaggggc aggcattcat gagtggcgct gcctcaaccg ggaagggccg attccggatg
     3481 gaaaatgcta aatatgaaac gctggatctg agcgacgaga atcaaaggaa tgactatctt
     3541 aagaattggg gatggcgtga cgagaagggg ctcgaggaac tgaagaaacg actgaactca
     3601 ggtctgccag agcccggtaa ttatagggat ccaaaatggA CAAAAGTAGG GTATGAGATT
     3661 AAAATACAGT CGCCCCTTCT TAGCAACGAT CCTATCGCAG CATTGTTCGA CACTGCAAAC
     3721 CACGACGCGG TCGCGTATAA GAAACGAGTG TGGGAGAACG GACGTATCAT AGAGCGACAC
     3781 ACTTTCAAAG GAGAGTCTAT ACGAGGGGTG CTGCGCACTG CACTTGGGCG TGAGTACGGA
     3841 TTGTTCGAAT TAAAACACGA AGACTGTCCT TGTAGTCTTT GCACAATTTT CGGTAACGAA
     3901 CACGAAGCGG GCAAAATACG TTTCGAAGAC TTAGAAATCG AGGACGGTGT TGCCGAAAAG
     3961 CACTTCGATC ACGTTGCTAT CGATCGCTTC ACAGGCGGAG CCGTAGACAA GAAGAAATTC
     4021 GACGATTTCT CTTTAGCTGG TTCCCCGGGA AAGCCCATTA AACTCAAAGG GTGCTTTTGG
     4081 TTGCGTGCGG ATATATTTAG TGACGAGAAG AAGGACGAGA AGAAGAAAAT AATAAACGCT
     4141 CTTTGTGACG TGCGTGATGG CTTGTACCCT CTCGGTGGCA AGGGCGGTGT CGGATACGGG
     4201 TGGGTAAAAG AGCTGAGAAT AACGGACGGC CCAGACGATT TTAAGCTCCC GGAACCGAAA
     4261 TGTAAGGAAA TTCCGCCAGA CGGTACTCCA AGCGATTACC CTTTTCCGCA GTTAAAGCCA
     4321 CTGCAAACAG AGAAGGATAA AGTTTACTCG CCCCACTACT TTCTGAAACC AGCAGAGAAG
     4381 AAAGTTGACC GGAACTGGGA AAAGCGGCTG ATCGGACACG AGCGTTTCGA TAAGGACCTG
     4441 TTGACTGGTA AAATTGAGTG TACACTGAAA ACGCTGAGCC CTTTAATAAT ACCGGACAGT
     4501 GAGGTCGTTA CGGAGAACGG AAACGGCCAC AAGTCTTACC AGTTCTTCAG ATTAAATAAT
     4561 GAAATCATCA TTCCGGGAAG CGAAATTCGG GGTATGATCA GTTCCGTGTA CGAAGCACTT
     4621 ACTAACTCTT GCTTCCGTGT CGTGGACGAC ATCATCCCGG TGGTGATCTC CCGTAGCGTG
     4681 TCACCGTACC AGATCGCTCA GAAGCTGCCC AGTCCGGATC TGCGTCCTTG CGAGCGTGAG
     4741 ATTCTTGAGG ACTCAGAAAT TGGATCTTTA GACAAGTGTT CAGAGAAACG CCTTTTCCGC
     4801 CTGCATGAAG ACGGTCTTTG CCCATCGTGT CGTCTGTTCG GCACTACACA TTATAAGGGA
     4861 CGCGTTCGGT TCGGCTTCGC AAAGCACGAG GGCGGCGAAA AGTGGCTCAT GGACGACAAG
     4921 ACTAAGGACG AGTATCTGAC TTTACCGTTG TTGGAACGGC CGCGCCCAAC ATGGAGCATG
     4981 CCAAGCGACG AAGACGACGT ACCGGGACGG AAATTCTACG TACACCACAA TGGTTGGGAG
     5041 AAAGTTAAGC AAGATAGTTT AGATAATAAA ACGAGCAAAA CCGAGAATAA TCGAAGCGTG
     5101 GAACCACTCG ATAAGGATAA CCTGTTTAAA TTCGACGTGT TCTTCGAGAA CCTGGAACCC
     5161 TGGGAACTTG GTTTGCTTCT CTACTCATTA GAATTGGAAG AGGGGTTAGC CCACAAGTTT
     5221 GGAATGGCAA AAGCGTTCGG CTTCGGCTCG ACAAAGATTG ACGCCGACAA AATACTGTTA
     5281 CGTGAGGCTC CGAACGAGTG GCAAGATGCT ACCAATCGTA AGGACGACAT GTTACGCAAG
     5341 GGCTTCGGCG AGCTGACATC CTGGTTCGAC AAGGATTGGG ACAAAGTCGA GCACATAGAC
     5401 GGTCTGCGTT CCTTACTTCA GATTCCGGAA GCGCTTTCAA GAGCCGAAGT GCGCTACCCG
     5461 GACCTTACGG AAGACGACCC AGAGAAGCCC CCGGGCTACG TGCAATTGTC TGATAAGAAA
     5521 GAGGGCCGCA AGTACTTGAT CCGTCCCTGG CAACCTTGGT ACTAAaccgg tcatcatcac
     5581 catcaccatt gagtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     5641 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     5701 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     5761 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     5821 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctcgataccg
     5881 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     5941 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctaggat
     6001 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     6061 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcgggaag aggcggtttg
     6121 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     6181 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     6241 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     6301 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     6361 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     6421 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     6481 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     6541 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     6601 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     6661 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     6721 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     6781 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     6841 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     6901 caagaagatc ctttgatctt ttctacgggg tctgacactc agtggaacga aaactcacgt
     6961 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     7021 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     7081 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     7141 tgactccccg tcgtgtagat aactacgat
//