LOCUS       CMV-SynCas.v39-bGH-AmpR 7127 bp ds-DNA     circular     13-SEP-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        complement(766..870)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     enhancer        1140..1519
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     promoter        1520..1723
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    1765..2478
                     /label="Cas7.1"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1765..2541
                     /label="Cas7-11 Frag"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    1765..3639
                     /label="DiCas7-11 Frag"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1765..5523
                     /label="SynCas.v52"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             1765..5523
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /translation="MTTTMKISIEFLEPFRMTKWQESTRRNKNNKEFVRGQAFARWHRNKKDNTKGRPYITGTLLRSAVIRSAENLLTLSDGKISEKTCCPGKFDTEDKDRLLQLRQRSTLRWTDKNPCPDNAETYCPFCELLGRSGNDGKKAEKKDWRFRIHFGNLSLPGKPDFDGPKAIGSQRVLNRVDFKSGKAHDFFKAYEVDHTRFPRFEGEITIDNKVSAEARKLLCDSLKFTDRLCGALCVIRFDEYTPAADSGKQTENVQAEPNANLAEKTAEQIISILDDNKKTEYTRLLADAIRSLRRSSKLVAGLPKDHDGKDDHYLWDIGKKKKDENSVTIRQILTTSADTKELKNAGKWREFCEKLGEALYLKSKDMSGGLKITRRILGDAEFHGKPDRLEKSRSVSIGSVLKETVVCGELVAKTPFFFGAIDEDAKQTDLQVLLTPDNKYRLPRSAVRGILRRDLQTYFDSPCNAELGGRPCMCKTCRIMRGITVMDARSEYNAPPEIRHRTRINPFTGTVAEGALFNMEVAPEGIVFPFQLRYRGSEDGLPDALKTVLKWWAEGQAFMSGAASTGKGRFRMENAKYETLDLSDENQRNDYLKNWGWRDEKGLEELKKRLNSGLPEPGNYRDPKWTKVGYEIKIQSPLLSNDPIAALFDTANHDAVAYKKRVWENGRIIERHTFKGESIRGVLRTALGREYGLFELKHEDCPCSLCTIFGNEHEAGKIRFEDLEIEDGVAEKHFDHVAIDRFTGGAVDKKKFDDFSLAGSPGKPIKLKGCFWLRADIFSDEKKDEKKKIINALCDVRDGLYPLGGKGGVGYGWVKELRITDDDKRISRLMNPAFDETDVAVPEKPKTDAEVRIEAEKVYYPHYFVEPHKKVEREEKPCGHQKFHEGRLTGKIECTLKTLSPLIIPDSEVVTENGNGHKSYQFFRLNNEIIIPGSEIRGMISSVYEALTNSCFRVVDDIIPVVISRSVSPYQIAQKLPSPDLRPCEREILEDSEIGSLDKCSEKRLFRLHEDGLCPSCRLFGTTHYKGRVRFGFAKHEGGEKWLMDDKTKDEYLTLPLLERPRPTWSMPSDEDDVPGRKFYVHHNGWEKVKQDSLDNKTSKTENNRSVEPLDKDNLFKFDVFFENLEPWELGLLLYSLELEEGLAHKFGMAKAFGFGSTKIDADKILLREEAGKFHPCEKADEYLKKGLNKLTSWFGKNEINEHMRNLLLFMTYYQNLPKVKYPDFDGYAKWRCSYVEQDKVEYFQNRWIVAS*"
     misc_feature    2479..2541
                     /label="Linker 1"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2542..2859
                     /label="Cas11"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2860..2967
                     /label="Linker 2"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2968..3510
                     /label="Cas7.2"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3511..3639
                     /label="Linker 3"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    3640..4227
                     /label="Cas7.3"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3640..4227
                     /label="Cas7-1 Frag"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    4228..4428
                     /label="DiCas7-11 Frag"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    4228..4428
                     /label="Linker 4"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    4429..4656
                     /label="Cas7.4-1"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    4429..5268
                     /label="Cas7-1 Frag"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    4657..4674
                     /label="INS"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    4675..5268
                     /label="Cas7.4-2"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    5269..5520
                     /label="CTE"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    5269..5520
                     /label="HvsCas7-11 Frag"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             5530..5547
                     /label="6xHis"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     polyA_signal    5576..5800
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     complement(5871..5887)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     protein_bind    5895..5911
                     /label="lac operator"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        complement(5919..5949)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     protein_bind    5964..5985
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     rep_origin      complement(6273..6861)
                     /label="ori"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             complement(7032..765)
                     /label="AmpR"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
ORIGIN
        1 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
       61 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
      121 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
      181 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
      241 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
      301 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
      361 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
      421 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
      481 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
      541 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
      601 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
      661 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
      721 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
      781 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
      841 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
      901 cgacggatcg ggagatcgat ctcccgatcc cctagggtcg actctcagta caatctgctc
      961 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
     1021 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
     1081 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
     1141 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     1201 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     1261 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     1321 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     1381 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     1441 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     1501 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     1561 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     1621 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     1681 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca
     1741 gatccgctag agatccgcgg ccgcatgacg actactatga agatttcaat tgaattcctc
     1801 gagccgtttc ggatgaccaa atggcaggaa agcactaggc gaaacaagaa caacaaggaa
     1861 tttgttcgcg gccaagcttt tgcaagatgg catagaaata agaaggataa cacaaaaggg
     1921 cggccttaca taactgggac attgctgcgg tccgcagtaa ttagaagcgc ggaaaacctg
     1981 ctgacattga gtgatgggaa aattagcgaa aagacatgtt gtcctgggaa atttgatacg
     2041 gaagataaag acaggttgct gcaattgagg cagcggtcca cccttcgttg gaccgataag
     2101 aacccctgtc cagataacgc tgaaacttac tgcccgtttt gcgaacttct cgggagatcc
     2161 ggtaacgatg gaaagaaggc tgagaagaag gattggcgct ttcgtattca ttttggcaat
     2221 ctttcccttc cagggaagcc cgatttcgac gggccaaaag ctataggcag ccaacgggta
     2281 cttaacaggg tcgatttcaa atccggtaag gcccatgact tctttaaggc ttacgaagtc
     2341 gaccatacta ggtttccccg cttcgaaggg gagattacca tagataataa agtcagcgct
     2401 gaagccagga aactgctctg tgattctctc aagtttacgg atcggctgtg tggagctctg
     2461 tgcgtaatca ggttcgatga atatacacca gcagccgata gtgggaaaca aaccgaaaat
     2521 gtccaagcag aaccgaatgc taatctcgct gaaaagaccg cagagcaaat tattagcatt
     2581 ctggacgata ataagaaaac cgaatatacc cgcctgcttg ctgatgctat tcgttcactc
     2641 cgccgctcta gtaaacttgt cgctggcttg ccaaaggatc atgacgggaa ggatgaccat
     2701 tacctctggg atatcggcaa gaagaagaaa gacgaaaact ctgtcactat taggcaaatc
     2761 cttacgacct cagcagacac caaggaactc aagaatgccg gaaaatggag agaattctgc
     2821 gagaagctgg gtgaagcgct gtacctcaag agtaaagaca tgagtggcgg cctgaaaatt
     2881 actcggagaa tactgggcga tgctgaattc catggaaagc ccgatcggct tgaaaagagc
     2941 cggtctgtgt caatcggatc tgtgttgaaa gaaactgtgg tatgcggcga actggtcgct
     3001 aaaacgccct tcttcttcgg agcgatagac gaagatgcaa aacaaaccga cctgcaagta
     3061 ctcctcactc ccgataacaa gtatagactg ccaagaagcg ccgtgcgagg tatactccgt
     3121 cgagatcttc aaacctattt tgatagccca tgcaatgctg agttgggtgg acggccatgc
     3181 atgtgtaaga cgtgtaggat tatgagaggg atcacggtga tggatgcgcg cagtgagtac
     3241 aatgccccgc cagaaataag gcatcgtacc cgcattaatc ccttcacagg cacggttgcg
     3301 gaaggtgccc tgtttaatat ggaagtagcc cccgagggga ttgtctttcc atttcaactc
     3361 cggtaccggg gctctgaaga tgggctgccc gatgcactga aaacggtgtt gaaatggtgg
     3421 gctgaggggc aggcattcat gagtggcgct gcctcaaccg ggaagggccg attccggatg
     3481 gaaaatgcta aatatgaaac gctggatctg agcgacgaga atcaaaggaa tgactatctt
     3541 aagaattggg gatggcgtga cgagaagggg ctcgaggaac tgaagaaacg actgaactca
     3601 ggtctgccag agcccggtaa ttatagggat ccaaaatggA CAAAAGTAGG GTATGAGATT
     3661 AAAATACAGT CGCCCCTTCT TAGCAACGAT CCTATCGCAG CATTGTTCGA CACTGCAAAC
     3721 CACGACGCGG TCGCGTATAA GAAACGAGTG TGGGAGAACG GACGTATCAT AGAGCGACAC
     3781 ACTTTCAAAG GAGAGTCTAT ACGAGGGGTG CTGCGCACTG CACTTGGGCG TGAGTACGGA
     3841 TTGTTCGAAT TAAAACACGA AGACTGTCCT TGTAGTCTTT GCACAATTTT CGGTAACGAA
     3901 CACGAAGCGG GCAAAATACG TTTCGAAGAC TTAGAAATCG AGGACGGTGT TGCCGAAAAG
     3961 CACTTCGATC ACGTTGCTAT CGATCGCTTC ACAGGCGGAG CCGTAGACAA GAAGAAATTC
     4021 GACGATTTCT CTTTAGCTGG TTCCCCGGGA AAGCCCATTA AACTCAAAGG GTGCTTTTGG
     4081 TTGCGTGCGG ATATATTTAG TGACGAGAAG AAGGACGAGA AGAAGAAAAT AATAAACGCT
     4141 CTTTGTGACG TGCGTGATGG CTTGTACCCT CTCGGTGGCA AGGGCGGTGT CGGATACGGG
     4201 TGGGTAAAAG AGCTGAGAAT AACGGACgat gataagagga tttctcgcct catgaatcct
     4261 gcctttgatg agacagatgt agccgttcca gaaaagccca aaactgatgc cgaggttcgc
     4321 atcgaggcag agaaagtata ttacccacac tatttcgtcg aaccccataa gaaggtggaa
     4381 cgcgaggaga aaccctgtgg tcatcaaaag ttccacgagg ggcgactgAC TGGTAAAATT
     4441 GAGTGTACAC TGAAAACGCT GAGCCCTTTA ATAATACCGG ACAGTGAGGT CGTTACGGAG
     4501 AACGGAAACG GCCACAAGTC TTACCAGTTC TTCAGATTAA ATAATGAAAT CATCATTCCG
     4561 GGAAGCGAAA TTCGGGGTAT GATCAGTTCC GTGTACGAAG CACTTACTAA CTCTTGCTTC
     4621 CGTGTCGTGG ACGACATCAT CCCGGTGGTG ATCTCCCGTA GCGTGTCACC GTACCAGATC
     4681 GCTCAGAAGC TGCCCAGTCC GGATCTGCGT CCTTGCGAGC GTGAGATTCT TGAGGACTCA
     4741 GAAATTGGAT CTTTAGACAA GTGTTCAGAG AAACGCCTTT TCCGCCTGCA TGAAGACGGT
     4801 CTTTGCCCAT CGTGTCGTCT GTTCGGCACT ACACATTATA AGGGACGCGT TCGGTTCGGC
     4861 TTCGCAAAGC ACGAGGGCGG CGAAAAGTGG CTCATGGACG ACAAGACTAA GGACGAGTAT
     4921 CTGACTTTAC CGTTGTTGGA ACGGCCGCGC CCAACATGGA GCATGCCAAG CGACGAAGAC
     4981 GACGTACCGG GACGGAAATT CTACGTACAC CACAATGGTT GGGAGAAAGT TAAGCAAGAT
     5041 AGTTTAGATA ATAAAACGAG CAAAACCGAG AATAATCGAA GCGTGGAACC ACTCGATAAG
     5101 GATAACCTGT TTAAATTCGA CGTGTTCTTC GAGAACCTGG AACCCTGGGA ACTTGGTTTG
     5161 CTTCTCTACT CATTAGAATT GGAAGAGGGG TTAGCCCACA AGTTTGGAAT GGCAAAAGCG
     5221 TTCGGCTTCG GCTCGACAAA GATTGACGCC GACAAAATAC TGTTACGTga agaggccggg
     5281 aagttccacc cttgcgagaa agcagacgaa taccttaaga agggcttgaa caagctgacc
     5341 agctggttcg gtaagaacga aatcaatgag cacatgcgaa acttactctt gttcatgact
     5401 tactatcaga accttcccaa ggttaagtac cctgattttg acggatacgc aaaatggcgg
     5461 tgcagctacg ttgaacagga caaggtggaa tactttcaga accgctggat tgtggcctct
     5521 TAAaccggtc atcatcacca tcaccattga gtttaaaccc gctgatcagc ctcgactgtg
     5581 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     5641 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     5701 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     5761 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     5821 agctggggct cgataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     5881 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     5941 aagtgtaaag cctaggatgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     6001 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     6061 gcgggaagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     6121 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     6181 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     6241 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     6301 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     6361 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     6421 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     6481 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     6541 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     6601 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     6661 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     6721 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     6781 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     6841 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacactcag
     6901 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     6961 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     7021 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     7081 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgat
//