LOCUS       pET28-H12TEV-hEIF5B-1~1220(FL)-Replacement.gb.xdna    9013 bp      DNA     circular   2024/3/11
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    5071..5169
                     /label=H12TEV
                     /SerialCloner_Color=&hFF8080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=6x his - thrombin - 6x his - TEV site  not continuous 12x his \ 
     misc_feature    5179..8841
                     /label=eIF5B 1~1220 (full length) [*]
                     /SerialCloner_Color=&h0080FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=human eIF5B (ENST00000289371) aa 1~1220 (full length) \ 
     variation       6743..6743
                     /label=rs7558074 (K522T)
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
                     /SerialCloner_Desc=https://www.ensembl.org/Homo_sapiens/Variation/Explore?db=core;g=ENSG00000158417;r=2:99337389-99401326;source=dbSNP;t=ENST00000289371;v=rs7558074 \ 
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt 
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 
      541 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat 
      601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa 
      661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc 
      721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga 
      781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc 
      841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac 
      901 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac 
      961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat 
     1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag 
     1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca 
     1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac 
     1201 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg 
     1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca 
     1321 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac 
     1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa 
     1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 
     1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 
     1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 
     1621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 
     1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 
     1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 
     1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 
     1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 
     1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 
     1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 
     2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 
     2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 
     2161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 
     2221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg 
     2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta 
     2341 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg 
     2401 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct 
     2461 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag 
     2521 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc 
     2581 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag 
     2641 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt 
     2701 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa 
     2761 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg 
     2821 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg 
     2881 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc 
     2941 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta 
     3001 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca 
     3061 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc 
     3121 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc 
     3181 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa 
     3241 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc 
     3301 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac 
     3361 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca 
     3421 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta 
     3481 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 
     3541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 
     3601 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca 
     3661 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa 
     3721 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt 
     3781 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg 
     3841 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca 
     3901 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta 
     3961 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg 
     4021 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat 
     4081 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct 
     4141 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg 
     4201 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat 
     4261 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc 
     4321 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca 
     4381 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg 
     4441 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt 
     4501 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg 
     4561 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct 
     4621 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga 
     4681 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg 
     4741 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc 
     4801 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg 
     4861 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg 
     4921 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga 
     4981 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa 
     5041 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac 
     5101 agcagcggcc tggtgccgcg cggcagccat ATGCATCACC ATCACCATCA CGAGAACCTG 
     5161 TACTTCCAAG GGGGATCCAT GGGGAAGAAA CAGAAAAACA AGAGCgaaga cagcaccaag 
     5221 gatgacattg atcttgatgc cttggctgca gaaatagaag gagctggtgc tgccaaagaa 
     5281 caggagcctc aaaagtcaaa agggaaaaag aaaaaagaga aaaaaaagca ggactttgat 
     5341 gaagatgata tcctgaaaga actggaagaa ttgtctttgg aagctcaagg catcaaagct 
     5401 gacagagaaa ctgttgcagt gaagccaaca gaaaacaatg aagaggaatt cacctcaaaa 
     5461 gataaaaaaa agaaaggaca gaagggcaaa aaacagagtt ttgatgataa tgatagcgaa 
     5521 gaattggaag ataaagattc aaaatcaaaa aagactgcaa aaccgaaagt ggaaatgtac 
     5581 tctgggagtg atgatgatga tgattttaac aaacttccta aaaaagctaa agggaaagct 
     5641 caaaaatcaa ataagaagtg ggatgggtca gaggaggatg aggataacag taaaaaaatt 
     5701 aaagagcgtt caagaataaa ttcttctggt gaaagtggtg atgaatcaga tgaatttttg 
     5761 caatctagaa aaggacagaa aaaaaatcag aaaaacaagc caggtcctaa catagaaagt 
     5821 gggaatgaag atgatgacgc ctccttcaaa attaagacag tggcccaaaa gaaggcagaa 
     5881 aagaaggagc gcgagagaaa aaagcgagat gaagaaaaag cgaaactgcg gaagctgaaa 
     5941 gaaaaagaag agttagaaac aggtaaaaag gatcagagta aacaaaagga atctcaaagg 
     6001 aaatttgaag aagaaactgt aaaatccaaa gtgactgttg atactggagt aattcctgcc 
     6061 tctgaagaga aagcagagac tcccacagct gcagaagatg acaatgaagg agacaaaaag 
     6121 aagaaagata agaagaaaaa gaaaggagaa aaggaagaaa aagagaaaga gaagaaaaaa 
     6181 ggacctagca aagccactgt taaagctatg caagaagctc tggctaagct taaagaggaa 
     6241 gaagaaagac agaagagaga agaggaagaa cgtataaaac ggcttgaaga attagaagcc 
     6301 aagcgtaaag aagaggaacg attggaacaa gaaaaaagag aaaggaaaaa gcaaaaagaa 
     6361 aaagaaagaa aagaacgctt gaaaaaagaa gggaaacttt taactaaatc ccagagagaa 
     6421 gccagagcca gagccgaagc tactcttaaa ctgctacaag ctcagggtgt tgaagtgcca 
     6481 tcaaaagact ctttgccaaa gaagaggcca atttatgaag ataaaaagag gaaaaaaata 
     6541 ccacagcagc tagaaagtaa agaagtgtct gaatcaatgg aattatgtgc tgctgtagaa 
     6601 gttatggaac aaggagtacc agaaaaggaa gagacaccac ctcctgttga accagaagaa 
     6661 gaagaagata ctgaggatgc tggattggat gattgggaag ctatggccag tgatgaggag 
     6721 acagaaaaag tagaaggaaa caCagttcat atagaagtaa aagaaaaccc tgaagaggag 
     6781 gaggaggagg aagaagagga agaagaagat gaagaaagtg aagaagagga ggaagaggag 
     6841 ggagaaagtg aaggcagtga aggtgatgag gaagatgaaa aggtgtcaga tgagaaggat 
     6901 tcagggaaga cattagataa aaagccaagt aaagaaatga gctcagattc tgaatatgac 
     6961 tctgatgatg atcggactaa agaagaaagg gcttatgaca aagcaaaacg gaggattgag 
     7021 aaacggcgac ttgaacatag taaaaatgta aacaccgaaa agctaagagc ccctattatc 
     7081 tgcgtacttg ggcatgtgga cacagggaag acaaaaattc tagataagct ccgtcacaca 
     7141 catgtacaag atggtgaagc aggtggtatc acacaacaaa ttggggccac caatgttcct 
     7201 cttgaagcta ttaatgaaca gactaagatg attaaaaatt ttgatagaga gaatgtacgg 
     7261 attccaggaa tgctaattat tgatactcct gggcatgaat ctttcagtaa tctgagaaat 
     7321 agaggaagct ctctttgtga cattgccatt ttagttgttg atattatgca tggtttggag 
     7381 ccccagacaa ttgagtctat caaccttctc aaatctaaaa aatgtccctt cattgttgca 
     7441 ctcaataaga ttgataggtt atatgattgg aaaaagagtc ctgactctga tgtggctgct 
     7501 actttaaaga agcagaaaaa gaatacaaaa gatgaatttg aggagcgagc aaaggctatt 
     7561 attgtagaat ttgcacagca gggtttgaat gctgctttgt tttatgagaa taaagatccc 
     7621 cgcacttttg tgtctttggt acctacctct gcacatactg gtgatggcat gggaagtctg 
     7681 atctaccttc ttgtagagtt aactcagacc atgttgagca agagacttgc acactgtgaa 
     7741 gagctgagag cacaggtgat ggaggttaaa gctctcccgg ggatgggcac cactatagat 
     7801 gtcatcttga tcaatgggcg tttgaaggaa ggagatacaa tcattgttcc tggagtagaa 
     7861 gggcccattg taactcagat tcgaggcctc ctgttacctc ctcctatgaa ggaattacga 
     7921 gtgaagaacc agtatgaaaa gcataaagaa gtagaagcag ctcagggggt aaagattctt 
     7981 ggaaaagacc tggagaaaac attggctggt ttacccctcc ttgtggctta taaagaagat 
     8041 gaaatccctg ttcttaaaga tgaattgatc catgagttaa agcagacact aaatgctatc 
     8101 aaattagaag aaaaaggagt ctatgtccag gcatctacac tgggttcttt ggaagctcta 
     8161 ctggaatttc tgaaaacatc agaagtgccc tatgcaggaa ttaacattgg cccagtgcat 
     8221 aaaaaagatg ttatgaaggc ttcagtgatg ttggaacatg accctcagta tgcagtaatt 
     8281 ttggccttcg atgtgagaat tgaacgagat gcacaagaaa tggctgatag tttaggagtt 
     8341 agaattttta gtgcagaaat tatttatcat ttatttgatg cctttacaaa atatagacaa 
     8401 gactacaaga aacagaaaca agaagaattt aagcacatag cagtatttcc ctgcaagata 
     8461 aaaatcctcc ctcagtacat ttttaattct cgagatccga tagtgatggg ggtgacggtg 
     8521 gaagcaggtc aggtgaaaca ggggacaccc atgtgtgtcc caagcaaaaa ttttgttgac 
     8581 atcggaatag taacaagtat tgaaataaac cataaacaag tggatgttgc aaaaaaagga 
     8641 caagaagttt gtgtaaaaat agaacctatc cctggtgagt cacccaaaat gtttggaaga 
     8701 cattttgaag ctacagatat tcttgttagt aagatcagcc ggcagtccat tgatgcactc 
     8761 aaagactggt tcagagatga aatgcagaag agtgactggc agcttattgt gGAGCTGAAG 
     8821 AAAGTATTTG AAATCATCTA Agcggccgca ctcgagcacc accaccacca ccactgagat 
     8881 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa 
     8941 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga 
     9001 actatatccg gat
//