LOCUS       pUC-T7_J1~398_Luc.gb.xdna    4734 bp      DNA     circular   2023/9/6
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=[DEFINITION]: synthetic DNA - [LOCUS]: pUC-T7/J1~398/Luc - [
            ACCESSION]: . - [SOURCE]: 1..4734
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     source          1..4734
                     /label=source
                     /SerialCloner_Color=&h2F4F4F
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=organism="synthetic DNA construct" - mol_type="other DNA" - plasmid="pUC-T7/J1~398/Luc" -  \ 
                     /SerialCloner_Desc=1..4734 \ 
     misc_feature    2080..4734
                     /label=pUC19_backbone
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="pUC19" - 2080..4734 \ 
     5'UTR           1..420
                     /label=T7/J1~398
                     /SerialCloner_Color=&hFF00FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="T7/HCV_JFH1_1~398" - 
1..420 \ 
     gene            421..2079
                     /label=firefly_Luciferase_gene
                     /SerialCloner_Color=&h0080FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="firefly_Luciferase" -  \ 
                     /SerialCloner_Desc=421..2079 \ 
     primer_bind     4715..31
                     /label=gene-specific primer: TAATACGACTCACTATAGACCTGCCC_spacer: aattc_overlap: ttgtaaaacgacggccagtg_gene-specific Tm: 66.8  Ta: 67.8_T7/J1~398_fwd_primer_bind
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="T7/J1~398_fwd" - note="gene-specific Tm: 66.8  Ta: 67.8" - note="overlap: ttgtaaaacgacggccagtg" - note="spacer: aattc" - note="gene-specific primer: TAATACGACTCACTATAGACCTGCCC" -  \ 
                     /SerialCloner_Desc=4715..31 \ 
     primer_bind     complement(403..430)
                     /label=gene-specific primer: TGGGCGACGGTTGGTGTT_overlap: cgtcttccat_gene-specific Tm: 70.6  Ta: 67.8_T7/J1~398_rev_primer_bind
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="T7/J1~398_rev" - note="gene-specific Tm: 70.6  Ta: 67.8" - note="overlap: cgtcttccat" - note="gene-specific primer: TGGGCGACGGTTGGTGTT" -  \ 
                     /SerialCloner_Desc=complement(403..430) \ 
     primer_bind     411..438
                     /label=gene-specific primer: ATGGAAGACGCCAAAAAC_overlap: ccgtcgccca_gene-specific Tm: 60.3  Ta: 61.3_firefly_Luciferase_fwd_primer_bind
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="firefly_Luciferase_fwd" - note="gene-specific Tm: 60.3  Ta: 61.3" - note="overlap: ccgtcgccca" - note="gene-specific primer: ATGGAAGACGCCAAAAAC" -  \ 
                     /SerialCloner_Desc=411..438 \ 
     primer_bind     complement(2056..2099)
                     /label=gene-specific primer: TTACACGGCGATCTTTCC_spacer: ctagaa_overlap: tgcatgcctgcaggtcgact_gene-specific Tm: 61.9  Ta: 61.3_firefly_Luciferase_rev_primer_bind
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="firefly_Luciferase_rev" - note="gene-specific Tm: 61.9  Ta: 61.3" - note="overlap: tgcatgcctgcaggtcgact" - note="spacer: ctagaa" - note="gene-specific primer: TTACACGGCGATCTTTCC" -  \ 
                     /SerialCloner_Desc=complement(2056..2099) \ 
ORIGIN
        1 aattctaata cgactcacta tagacctgcc cctaataggg gcgacactcc gccatgaatc 
       61 actcccctgt gaggaactac tgtcttcacg cagaaagcgc ctagccatgg cgttagtatg 
      121 agtgtcgtac agcctccagg cccccccctc ccgggagagc catagtggtc tgcggaaccg 
      181 gtgagtacac cggaattgcc gggaagactg ggtcctttct tggataaacc cactctatgc 
      241 ccggccattt gggcgtgccc ccgcaagact gctagccgag tagcgttggg ttgcgaaagg 
      301 ccttgtggta ctgcctgata gggcgcttgc gagtgccccg ggaggtctcg tagaccgtgc 
      361 accatgagca caaatcctaa acctcaaaga aaaaccaaaa gaaacaccaa ccgtcgccca 
      421 atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct ggaagatgga 
      481 accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt 
      541 gcttttacag atgcacatat cgaggtggac atcacttacg ctgagtactt cgaaatgtcc 
      601 gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta 
      661 tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt 
      721 gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgggcatt 
      781 tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa 
      841 aaaaagctcc caatcatcca aaaaattatt atcatggatt ctaaaacgga ttaccaggga 
      901 tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat 
      961 tttgtgccag agtccttcga tagggacaag acaattgcac tgatcatgaa ctcctctgga 
     1021 tctactggtc tgcctaaagg tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg 
     1081 catgccagag atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt 
     1141 gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt 
     1201 cgagtcgtct taatgtatag atttgaagaa gagctgtttc tgaggagcct tcaggattac 
     1261 aagattcaaa gtgcgctgct ggtgccaacc ctattctcct tcttcgccaa aagcactctg 
     1321 attgacaaat acgatttatc taatttacac gaaattgctt ctggtggcgc tcccctctct 
     1381 aaggaagtcg gggaagcggt tgccaagagg ttccatctgc caggtatcag gcaaggatat 
     1441 gggctcactg agactacatc agctattctg attacacccg agggggatga taaaccgggc 
     1501 gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa 
     1561 acgctgggcg ttaatcaaag aggcgaactg tgtgtgagag gtcctatgat tatgtccggt 
     1621 tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct 
     1681 ggagacatag cttactggga cgaagacgaa cacttcttca tcgttgaccg cctgaagtct 
     1741 ctgattaagt acaaaggcta tcaggtggct cccgctgaat tggaatccat cttgctccaa 
     1801 caccccaaca tcttcgacgc aggtgtcgca ggtcttcccg acgatgacgc cggtgaactt 
     1861 cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat 
     1921 tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac 
     1981 gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata 
     2041 aaggccaaga agggcggaaa gatcgccgtg taattctaga gtcgacctgc aggcatgcaa 
     2101 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 
     2161 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 
     2221 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 
     2281 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 
     2341 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 
     2401 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 
     2461 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 
     2521 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 
     2581 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 
     2641 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 
     2701 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 
     2761 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 
     2821 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 
     2881 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 
     2941 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct 
     3001 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 
     3061 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 
     3121 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 
     3181 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 
     3241 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 
     3301 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 
     3361 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 
     3421 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 
     3481 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 
     3541 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 
     3601 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 
     3661 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 
     3721 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 
     3781 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 
     3841 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 
     3901 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 
     3961 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 
     4021 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 
     4081 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 
     4141 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 
     4201 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 
     4261 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta 
     4321 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc 
     4381 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc 
     4441 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc 
     4501 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa 
     4561 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg 
     4621 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa 
     4681 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtg
//