LOCUS       pUC-T7-hTP53-P0~670-FLuc.gb.xdna    5003 bp      DNA     circular   2024/1/11
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=[DEFINITION]: synthetic DNA - [LOCUS]: pUC-T7-hTP53-P0~670/F
            
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     gene            2349..5003
                     /label=pUC19_backbone
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="pUC19" - 2466..5120 \ 
     5'UTR           1..136
                     /label=p53_P0~P1
                     /SerialCloner_Color=&hFF80FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="p53_P0~P1" - 1..136 \ 
     gene            137..689
                     /label=p53_P1~670 [*]
                     /SerialCloner_Color=&h8080C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="p53_P1~670" - 137..806 \ 
     gene            690..2348
                     /label=firefly_Luciferase_gene
                     /SerialCloner_Color=&h0080FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="firefly_Luciferase" - 807..2465 \ 
ORIGIN
        1 aattctaata cgactcacta tagtcatggc gactgtccag ctttgtgcca ggagcctcgc 
       61 aggggttgat gggattgggg ttttcccctc ccatgtgctc aagactggcg ctaaaagttt 
      121 tgagcttctc aaaagtCTAG AGCCACCGTC CAGGGAGCag gtagctgctg ggctccgggg 
      181 acactttgcg ttcgggctgg gagcgtgctt tccacgacgg tgacacgctt ccctggattg 
      241 gcagccagac tgccttccgg gtcactgcca tggaggagcc gcagtcagat cctagcgtcg 
      301 agccccctct gagtcaggaa acattttcag acctatggaa actacttcct gaaaacaacg 
      361 ttctgtcccc cttgccgtcc caagcaatgg atgatttgat gctgtccccg gacgatattg 
      421 aacaatggtt cactgaagac ccaggtccag atgaagctcc cagaatgcca gaggctgctc 
      481 cccccgtggc ccctgcacca gcagctccta caccggcggc ccctgcacca gccccctcct 
      541 ggcccctgtc atcttctgtc ccttcccaga aaacctacca gggcagctac ggtttccgtc 
      601 tgggcttctt gcattctggg acagccaagt ctgtgacttg cacgtactcc cctgccctca 
      661 acaaGATGTT TTGCCAACTG GCCAAGACCa tggaagacgc caaaaacata aagaaaggcc 
      721 cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat aaggctatga 
      781 agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc gaggtggaca 
      841 tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg aaacgatatg 
      901 ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc 
      961 cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac atttataatg 
     1021 aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc gtttccaaaa 
     1081 aggggttgca aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa aaaattatta 
     1141 tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc gtcacatctc 
     1201 atctacctcc cggttttaat gaatacgatt ttgtgccaga gtccttcgat agggacaaga 
     1261 caattgcact gatcatgaac tcctctggat ctactggtct gcctaaaggt gtcgctctgc 
     1321 ctcatagaac tgcctgcgtg agattctcgc atgccagaga tcctattttt ggcaatcaaa 
     1381 tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt ggaatgttta 
     1441 ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga tttgaagaag 
     1501 agctgtttct gaggagcctt caggattaca agattcaaag tgcgctgctg gtgccaaccc 
     1561 tattctcctt cttcgccaaa agcactctga ttgacaaata cgatttatct aatttacacg 
     1621 aaattgcttc tggtggcgct cccctctcta aggaagtcgg ggaagcggtt gccaagaggt 
     1681 tccatctgcc aggtatcagg caaggatatg ggctcactga gactacatca gctattctga 
     1741 ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca ttttttgaag 
     1801 cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcaaaga ggcgaactgt 
     1861 gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa tccggaagcg accaacgcct 
     1921 tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac 
     1981 acttcttcat cgttgaccgc ctgaagtctc tgattaagta caaaggctat caggtggctc 
     2041 ccgctgaatt ggaatccatc ttgctccaac accccaacat cttcgacgca ggtgtcgcag 
     2101 gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa 
     2161 agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa 
     2221 agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg 
     2281 acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag atcgccgtgt 
     2341 aattctagag tcgacctgca ggcatgcaag cttggcgtaa tcatggtcat agctgtttcc 
     2401 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 
     2461 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 
     2521 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 
     2581 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 
     2641 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 
     2701 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 
     2761 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 
     2821 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 
     2881 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 
     2941 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 
     3001 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 
     3061 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 
     3121 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 
     3181 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg 
     3241 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 
     3301 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 
     3361 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 
     3421 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 
     3481 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 
     3541 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 
     3601 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 
     3661 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 
     3721 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 
     3781 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 
     3841 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 
     3901 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 
     3961 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 
     4021 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 
     4081 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 
     4141 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 
     4201 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 
     4261 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 
     4321 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 
     4381 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 
     4441 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 
     4501 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat 
     4561 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg 
     4621 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta 
     4681 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg 
     4741 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg 
     4801 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag 
     4861 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc 
     4921 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg 
     4981 acgttgtaaa acgacggcca gtg
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