LOCUS       pUC-ODN-hsRPL3-CterTwStrep_assembledseq.gb.xdna    3150 bp      DNA     circular   2023/9/6
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=[DEFINITION]: synthetic DNA - [LOCUS]: pUC-ODN-hsRPL3-CterTw
            Strep - [ACCESSION]: . - [SOURCE]: 1..3150
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     source          886..885
                     /label=source
                     /SerialCloner_Color=&h2F4F4F
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=organism="synthetic DNA construct" - mol_type="other DNA" - plasmid="pUC-ODN-hsRPL3-CterTwStrep" -  \ 
                     /SerialCloner_Desc=1..3150 \ 
     gene            886..417
                     /label=pUC-ODN
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="pUC-ODN" -  \ 
                     /SerialCloner_Desc=1..2682 \ 
     gene            418..577
                     /label=5'_HomoArm_gene
                     /SerialCloner_Color=&h0080FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="5'_HomoArm" -  \ 
                     /SerialCloner_Desc=2683..2842 \ 
     gene            578..712
                     /label=RPL3_Ex10_and_Twin_Strep_gene
                     /SerialCloner_Color=&hFF8080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="RPL3_Ex10_and_Twin_Strep" -  \ 
                     /SerialCloner_Desc=2843..2977 \ 
     gene            713..885
                     /label=3'_HomoArm_gene
                     /SerialCloner_Color=&h0080FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="3'_HomoArm" -  \ 
                     /SerialCloner_Desc=2978..3150 \ 
     primer_bind     398..442
                     /label=gene-specific primer: CCGGGGTAGGGGTGGTCT_spacer: gctcttc_overlap: attcgagctcggtacccggg_gene-specific Tm: 72.4  Ta: 68.7_5'_HomoArm_fwd_primer_bind
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="5'_HomoArm_fwd" - note="gene-specific Tm: 72.4  Ta: 68.7" - note="overlap: attcgagctcggtacccggg" - note="spacer: gctcttc" - note="gene-specific primer: CCGGGGTAGGGGTGGTCT" -  \ 
                     /SerialCloner_Desc=2663..2707 \ 
     primer_bind     complement(545..587)
                     /label=gene-specific primer: CTGTGGGGAGAGAGGCAG_spacer: ctttttgagtggtcc_overlap: caattcggtc_gene-specific Tm: 67.7  Ta: 68.7_5'_HomoArm_rev_primer_bind
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="5'_HomoArm_rev" - note="gene-specific Tm: 67.7  Ta: 68.7" - note="overlap: caattcggtc" - note="spacer: ctttttgagtggtcc" - note="gene-specific primer: CTGTGGGGAGAGAGGCAG" -  \ 
                     /SerialCloner_Desc=complement(2810..2852) \ 
     primer_bind     563..596
                     /label=gene-specific primer: GACCGAATTGCAAAGGAAG_spacer: ggaccactcaaaaag_gene-specific Tm: 61.3  Ta: 59.7_RPL3_Ex10_and_Twin_Strep_fwd_primer_bind
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="RPL3_Ex10_and_Twin_Strep_fwd" - note="gene-specific Tm: 61.3  Ta: 59.7" - note="spacer: ggaccactcaaaaag" - note="gene-specific primer: GACCGAATTGCAAAGGAAG" -  \ 
                     /SerialCloner_Desc=2828..2861 \ 
     primer_bind     complement(694..722)
                     /label=gene-specific primer: TTACTTCTCAAATTGGGGG_overlap: gttcctggca_gene-specific Tm: 58.7  Ta: 59.7_RPL3_Ex10_and_Twin_Strep_rev_primer_bind
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="RPL3_Ex10_and_Twin_Strep_rev" - note="gene-specific Tm: 58.7  Ta: 59.7" - note="overlap: gttcctggca" - note="gene-specific primer: TTACTTCTCAAATTGGGGG" -  \ 
                     /SerialCloner_Desc=complement(2959..2987) \ 
     primer_bind     703..730
                     /label=gene-specific primer: TGCCAGGAACAGATTTTG_overlap: tgagaagtaa_gene-specific Tm: 59.7  Ta: 60.7_3'_HomoArm_fwd_primer_bind
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="3'_HomoArm_fwd" - note="gene-specific Tm: 59.7  Ta: 60.7" - note="overlap: tgagaagtaa" - note="gene-specific primer: TGCCAGGAACAGATTTTG" -  \ 
                     /SerialCloner_Desc=2968..2995 \ 
     primer_bind     complement(861..905)
                     /label=gene-specific primer: CCAGCGAAGCTACAGAAAG_spacer: gcaatg_overlap: cctgcaggtcgactctagag_gene-specific Tm: 63.0  Ta: 60.7_3'_HomoArm_rev_primer_bind
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="3'_HomoArm_rev" - note="gene-specific Tm: 63.0  Ta: 60.7" - note="overlap: cctgcaggtcgactctagag" - note="spacer: gcaatg" - note="gene-specific primer: CCAGCGAAGCTACAGAAAG" -  \ 
                     /SerialCloner_Desc=complement(3126..20) \ 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggct 
      421 cttcccgggg taggggtggt cttgatgcct taggcctctc ttgtggctgt ggagactggc 
      481 ccagagtctt ggtctcctct agagtgggca tctgctgtca cctgtcatct tctacctctg 
      541 accactgcct ctctccccac agggaccact caaaaaggac cgaattgcaa aggaagaagg 
      601 agctaagctt tccggaggga gcgcttggag ccacccgcag ttcgaaaaag gtggaggttc 
      661 tggcggtgga tcgggaggtt cagcgtggtc tcacccccaa tttgagaagt aatgccagga 
      721 acagattttg cagttggtgg ggtctcaata aaagttattt tccactgaca tcttgcctct 
      781 ttcttcagga gagaggctgg gactggggag gcagatgggg gcccttgagg tgccttgtgc 
      841 tccccaaggc cactgccctc ctttctgtag cttcgctggc attgcctcta gagtcgacct 
      901 gcaggcatgc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 
      961 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 
     1021 aatgagtgag ctaactcaca ttaattgcgt tgcgctcaca gcccgctttc cagtcgggaa 
     1081 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 
     1141 ttgggcgctg ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 
     1201 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 
     1261 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 
     1321 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 
     1381 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 
     1441 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 
     1501 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 
     1561 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 
     1621 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 
     1681 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 
     1741 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 
     1801 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 
     1861 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 
     1921 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 
     1981 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 
     2041 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 
     2101 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 
     2161 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 
     2221 taataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 
     2281 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 
     2341 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 
     2401 tagctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 
     2461 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 
     2521 tcggtcctcc gatcgttgtc agaagtaagt tggccgctgt gttatcactc atggttatgg 
     2581 cagcactaca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 
     2641 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 
     2701 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 
     2761 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 
     2821 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 
     2881 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 
     2941 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 
     3001 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 
     3061 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata 
     3121 aaaataggcg tatcacgagg ccctttcgtc 
//