LOCUS       Exported                4475 bp ds-DNA     circular SYN 06-SEP-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 6, 2023 from SnapGene 4.1.4
            http://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    304..361
                     /label=atl terminator
                     /note="atl terminator from S. aureus"
                     /note="color: #ff0000"
     misc_feature    444..481
                     /label=MCS
                     /note="Multiple cloning site designed by authors (Krute, et
                     al., 2016)"
                     /note="color: #a6acb3"
     misc_feature    488..787
                     /label=blaZ terminator
                     /note="blaZ transcription terminator from S. aureus blaZ 
                     gene"
                     /note="color: #ff0000"
     CDS             complement(825..1559)
                     /codon_start=1
                     /product="ermC coding sequence within the erythromycin 
                     resistance fragment, found via UniProt and genebank on 
                     pE194 open reading frames"
                     /label=ermC
                     /note="color: #ff6600"
                     /translation="MNEKNIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHFT
                     LELVKRCNFVTAIEIDHKLCKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIYGNIPY
                     NISTDIIRKIVFDSIANEIYLIVEYGFAKRLLNTKRSLALLLMAEVDISILSMVPREYF
                     HPKPKVNSSLIRLSRKKSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKHAGID
                     DLNNISFEQFLSLFNSYKLFNK"
     misc_feature    complement(1570..1753)
                     /label=sarA P1 promoter fragment
                     /note="Constitutive promoter used in S. aureus"
                     /note="color: #3366ff; direction: LEFT"
     misc_feature    complement(1636..1668)
                     /label=sarA P1 promoter
                     /note="sarA P1 promoter from S. aureus as given on iGEM 
                     (part BBa_K1323021). Within the fragment used to create 
                     this vector. 
                     Transcription start site is directly after the end of this 
                     feature (Bayer, et al., 1996)"
                     /note="color: #a6acb3; direction: LEFT"
     rep_origin      complement(1775..2363)
                     /direction=LEFT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
                     /note="color: #ffff00"
     misc_feature    2566..3561
                     /label=repF
                     /note="Replication gene for plasmid replication in S. 
                     aureus (Krute, 2016). Plasmid replicates in S. aureus using
                     the rolling circle mechanism. 
                     Very stable replication in S. aureus without antibiotic 
                     selection (nearly 100% over 100 generations; Krute, 2016)"
                     /note="color: #ff9900"
     CDS             complement(2575..3504)
                     /label=rep
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=52.4"
                     /note="/match_length=97.8"
                     /note="/other=CDS"
                     /note="color: #993366; direction: LEFT"
     misc_feature    3716..3736
                     /label=dso
                     /note="Double strand origin of replication of pKK30 
                     (derived from LAC-p1 plasmid) for replication in S. aureus 
                     (Krute, 2016). Important for plasmid stability and 
                     beginning of plasmid replication"
                     /note="color: #a6acb3"
     misc_feature    4205..4382
                     /label=sso
                     /note="Single strand replication origin for replication of 
                     pKK30 (derived from LAC-p1 plasmid) in S. aureus (Krute, 
                     2016). Important for plasmid stability (rolling circle 
                     replication)"
                     /note="color: #a6acb3"
ORIGIN
        1 aaaaaaacag tgtgaaaaca agaagaaact cgtttcaaaa tcccctcaaa tattcccctc
       61 gagttacatg caaattaaaa attagcataa aatcctttaa aacttccaca tgtttttcta
      121 aattcctgca gggcgttttt tatcaaaaat cgagaatttt taatttttct ctaaaaactc
      181 cgtaagagca taaggagaat ttgctatcat tcaaaaatga taggtctggc aaagccagaa
      241 attccagggg ttttaaggta ttttaaagtg atcaaaggcg attaaataag caacatgaac
      301 ataggatcaa aagtcatccc cccactatca atcatggggg atgacctttg atcccttttt
      361 tatacataca caagcaaaaa tagcggtgat tgtttaccat caattttaac aatcaccgct
      421 acttttgctt gtaattcatg attcggatcc ccgggtaccg agctcctagg ctagcggccg
      481 catcgcctgt cactttgctt gatatatgag aattatttaa ccttataaat gagaaaaaag
      541 caacgcactt taaataagat acgttgcttt ttcgattgat gaacacctat aattaaacta
      601 ttcatctatt atttataatt ttttgtatat acaatatttc tagtttgtta aagagaatta
      661 agaaaataaa tctcgaaaat aatagaggga aaatcagttt ttgatatcaa aattatacat
      721 gtcaacgata atacaaaata taatacaaaa tataagatgt tatcagtatt tattatgcat
      781 tagaataggc gcgcctattt cccttttcta cggcatccct taacttactt attaaataat
      841 ttatagctat tgaaaagaga taagaattgt tcaaagctaa tattgtttaa atcgtcaatt
      901 cctgcatgtt ttaaggaatt gttaaattga ttttttgtaa atattttctt gtattctttg
      961 ttaacccatt tcataacgaa ataattatac ttttgtttat ctttgtgtga tattcttgat
     1021 ttttttctac ttaatctgat aagtgagcta ttcactttag gtttaggatg aaaatattct
     1081 cttggaacca tacttaatat agaaatatca acttctgcca ttaaaagtaa tgccaatgag
     1141 cgttttgtat ttaataatct tttagcaaac ccgtattcca cgattaaata aatctcatta
     1201 gctatactat caaaaacaat tttgcgtatt atatccgtac ttatgttata aggtatatta
     1261 ccatatattt tataggattg gtttttagga aatttaaact gcaatatatc cttgtttaaa
     1321 acttggaaat tatcgtgatc aacaagttta ttttctgtag ttttgcataa tttatggtct
     1381 atttcaatgg cagttacgaa attacacctc tttactaatt caagggtaaa atggcctttt
     1441 cctgagccga tttcaaagat attatcatgt tcatttaatc ttatatttgt cattatttta
     1501 tctatattat gttttgaagt aataaagttt tgactgtgtt ttatattttt ctcgttcatt
     1561 ataaccctca gatatgttag tcataatttt gcattaaata agttttatta aatatattta
     1621 atgctctatt atttagttaa ttataactaa ttaaaaatga gaagtaaaca aaaaagtgtt
     1681 tataaaacaa attattcatt agacacagtg attgtatttc tgggttagca tttggtttag
     1741 tcaaaaatat cagctcgaat tcccatgctc cgtttttcca taggctccgc ccccctgacg
     1801 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     1861 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     1921 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     1981 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     2041 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     2101 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     2161 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     2221 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     2281 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     2341 cgcgcagaaa aaaaggatct caagaagatc ctttgatctc acgctctacg ccggacgcat
     2401 cgtggccagg ctttcgtcga cggatcatac aaagctgtac tgtcttattt ttgtgacaca
     2461 atatattgcg tttgtcttat ttttgtgaca ctacatacag catttgtcac aaaaataaga
     2521 cagtactttt ttataaaaaa tcaacaaaaa agacattagt caacactaat gtcttaaata
     2581 ataattttgc ttttcgaaat tccaaattgc ggtaattgaa tgtgcttttt cttcttcgtc
     2641 tgttgtttta tcttcatcac ttgcattaat caaattacca tcttcgacat cgtctaagtt
     2701 taaaatttta tgtttttgtt taagcaatcc accataactt aacatacgtt ttcgatacaa
     2761 acctttttct aaatcactta caatttcttg atttttttca tcatcatcag ttaaaaaatc
     2821 agatgattta accgaatatt ttgaggtctc tttgattgcc gattcaatgt ctttatcgcc
     2881 ttttctattc ggtttgatcg ctttaacatt agcaacaggt cgatagtcaa cttgtaatgc
     2941 tctttgccat aaattaaccc attcttcttg agttatataa ttctcttttt ttctaaaata
     3001 tgcattttca acacataaca aaacatgcat gtgctgatta taactaccgt catttttatt
     3061 aacggtaact tctgttgaac gcataaatcc aacaagattt tgtttaacct ttttatatct
     3121 actcaaccta tcaaatgctt tagttagatg cttcaaactt tgttctaaag tatctccatc
     3181 tatcgcattt tttgttgaaa gtgttaaaaa caaccaacgt gcttttggct tttccttaat
     3241 tactttttca atcacttttt gagcttgata actatttttc atagcacgtc tccaattaca
     3301 aaccggacat aattttgatt tacaaaacca tgtcttatgt aacttcaaat agccttcatc
     3361 agttggctta aattctaaaa tattaccaca ttgttttaca ttaaaagcct ttttgatttt
     3421 taaaatttct agtatatctg catagcttac attatctatt ttcttttctc tccatgggcg
     3481 ttgtttccca cttttcgtca tatcttttaa cgttttatta tcttgatttt cggttatact
     3541 tctagtagta ttatattgca tataaaaaga cccccagtat tatttaagat tcgacacctt
     3601 aataatataa ctggggtttt ttattgtcaa attttccttt cacgcacaaa aacacgttta
     3661 aacactgtaa tatcaacagt tacagctata tatataaaat cgagttattt ctatatagta
     3721 tcaagacaag aagaaactcg ttcacactcg tttcaaaaaa caacctattc cttcttcttt
     3781 ttatgaccct ctaaacctaa ataaattaat gtgaaaacca atgaataaat aaaactttct
     3841 aaaaagtcaa aaggattatc aaataaaatc ggccaaatct cgatatggat atgttcctga
     3901 aggattccaa aaactaaaaa acgacataga gaataaaaag aaaactagtt caatatcacc
     3961 tagagcagtt ggtgggaact acaaaaatag cggtgactgt ttctattctg aagttttaaa
     4021 ctcatatgga gaacttttaa ctggaaacat aatcacagca gtttttgaag atgttaaagc
     4081 caaaaactta aagggcgtag caaaaaaatt agctaaaatt gggataaaag gaaacctagc
     4141 tggaatcgca gctactatgg tttcgaagga tgtacaatgt aatcttaaat acgggttact
     4201 ttagatacta tttattccaa ttgctttatt gacgttgagc ctcggaaccc ttaacaatcc
     4261 caaaacttgt cgaatggtcg gcttaatagc tcacgctatg ccgacattcg tctgcaagtt
     4321 tagttaaggg ttcttctcaa catcaataaa ttttctcggc ataaatgcgt cgtctatttt
     4381 ttattatctt catgataatc atatttttct tccatctcgt caaaagcatc atctttatag
     4441 aaaaacaaaa agtaagcaac aacaccaata ggaat
//
