LOCUS       Exported                6565 bp ds-DNA     circular SYN 06-SEP-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6565)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 6, 2023 from SnapGene 4.1.4
            http://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..6565
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             222..869
                     /label=cat
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366; direction: RIGHT"
     promoter        1896..2000
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff"
     CDS             2001..2861
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366; direction: RIGHT"
     rep_origin      3032..3620
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
                     /note="color: #ffff00"
     protein_bind    3908..3929
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
                     /note="color: #31849b"
     promoter        3944..3974
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff"
     protein_bind    3982..3998
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
                     /note="color: #31849b"
     CDS             complement(4040..4057)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     CDS             complement(4064..4777)
                     /label=Superfolder GFP
                     /note="pLannotate"
                     /note="/database=fpbase"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366; direction: LEFT"
     misc_feature    complement(4811..4900)
                     /label=sarA P1 promoter fragment
                     /note="Constitutive promoter used in S. aureus"
                     /note="color: #3366ff; direction: LEFT"
     misc_feature    complement(4905..4947)
                     /label=PSarA1
                     /note="color: #0000ff; direction: LEFT"
     misc_feature    complement(4952..5013)
                     /label=sarA P1 promoter fragment
                     /note="Constitutive promoter used in S. aureus"
                     /note="color: #3366ff; direction: LEFT"
     misc_feature    5297..1405
                     /label=pC194_replicon
                     /note="color: #ffff00"
     CDS             complement(5394..6329)
                     /codon_start=1
                     /product="The pC194 ORI for S. aureus for replication 
                     (rolling circle replication) of the plasmid in S. aureus. 
                     Middle to high copy number. 
                     Because this is a rolling circle plasmid, stability may be 
                     lost when the plasmid is greater than about 9 kb 
                     Includes the rep protein but likely doesn't include the 
                     double and single origin sequences that are likely present 
                     shortly before this gene, so be careful if inserting 
                     sequences directly before this gene. "
                     /label=pC194 ORI
                     /note="The pC194 ORI for S. aureus for replication of the 
                     plasmid in S. aureus. Includes the "
                     /note="color: #ffcc00"
                     /translation="MCYNMEKYTEKKQRNQVFQKFIKRHIGENQMDLVEDCNTFLSFVA
                     DKTLEKQKLYKANSCKNRFCPVCAWRKARKDALGLSLMMQYIKQQEKKEFIFLTLTTPN
                     VMSDELENEIKRYNNSFRKLIKRKKVGSVIKGYVRKLEITYNKKRDDYNPHFHVLIAVN
                     KSYFTDKRYYISQQEWLDLWRDVTGISEITQVQVQKIRQNNNKELYEMAKYSGKDSDYL
                     INQKVFDAFYKSLKGKQVLVYSGLFKEAKKKLKNGDLDYLKEIDPTEYIYQIFYIWKQK
                     EYLASELYDLTEQEKREINHKMIDEIEEEQ"
ORIGIN
        1 aaaaaaacac aaaagaccac attttttaat gtggtctttt attcttcaac taaagcaccc
       61 attagttcaa caaacgaaaa ttggataaag tgggatattt ttaaaatata tatttatgtt
      121 acagtaatat tgacttttaa aaaaggattg attctaatga agaaagcaga caagtaagcc
      181 tcctaaattc actttagata aaaatttagg aggcatatca aatgaacttt aataaaattg
      241 atttagacaa ttggaagaga aaagagatat ttaatcatta tttgaaccaa caaacgactt
      301 ttagtataac cacagaaatt gatattagtg ttttataccg aaacataaaa caagaaggat
      361 ataaatttta ccctgcattt attttcttag tgacaagggt gataaactca aatacagctt
      421 ttagaactgg ttacaatagc gacggagagt taggttattg ggataagtta gagccacttt
      481 atacaatttt tgatggtgta tctaaaacat tctctggtat ttggactcct gtaaagaatg
      541 acttcaaaga gttttatgat ttataccttt ctgatgtaga gaaatataat ggttcgggga
      601 aattgtttcc caaaacacct atacctgaaa atgctttttc tctttctatt attccatgga
      661 cttcatttac tgggtttaac ttaaatatca ataataatag taattacctt ctacccatta
      721 ttacagcagg aaaattcatt aataaaggta attcaatata tttaccgcta tctttacagg
      781 tacatcattc tgtttgtgat ggttatcatg caggattgtt tatgaactct attcaggaat
      841 tgtcagatag gcctaatgac tggcttttat aatatgagat aatgccgact gtacttttta
      901 cagtcggttt tctaatgtca ctaacctgcc ccgttagttg aagaaggttt ttatattaca
      961 gctccagatc catatccttc tttttctgaa ccgacttctc ctttttcgct tctttattcc
     1021 aattgcttta ttgacgttga gcctcggaac ccttaacaat cccaaaactt gtcgaatggt
     1081 cggcttaata gctcacgcta tgccgacatt cgtctgcaag tttagttaag ggttcttctc
     1141 aacgcacaat aaattttctc ggcataaatg cgtggtctaa tttttatttt taataacctt
     1201 gatagcaaaa aatgccattc caatacaaaa ccacatacct ataatcgata accacataac
     1261 agtcataaaa ccactccttt ttaacaaact ttatcacaag aaatatttaa attttaaatg
     1321 cctttatttt gaattttaag gggcatttta aagatttagg ggtaaatcat atagttttat
     1381 gcctaaaaac ctacagaagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     1441 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     1501 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     1561 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     1621 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     1681 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     1741 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     1801 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     1861 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     1921 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     1981 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     2041 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     2101 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     2161 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     2221 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     2281 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     2341 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     2401 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     2461 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     2521 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     2581 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     2641 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     2701 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     2761 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     2821 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     2881 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     2941 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     3001 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     3061 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     3121 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     3181 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3241 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3301 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3361 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3421 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3481 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3541 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3601 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3661 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3721 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     3781 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     3841 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     3901 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     3961 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     4021 acatgattac gaattcttag tggtggtggt ggtggtggga tcctttgtag agctcatcca
     4081 tgccatgtgt aatcccagca gcagttacaa actcaagaag gaccatgtgg tcacgctttt
     4141 cgttaggatc tttcgaaagg acagattgtg tcgacaggta atggttgtct ggtaaaagga
     4201 cagggccatc gccaattgga gtattttgtt gataatggtc tgctagttga acggaaccat
     4261 cttcaacgtt gtggcgaatt ttgaagttag ctttgattcc attcttttgt ttgtctgccg
     4321 tgatgtatac attgtgtgag ttaaagttgt actcgagttt gtgtccaaga atgtttccat
     4381 cttctttaaa atcaataccc tttaactcga tacgattaac aagggtatca ccttcaaact
     4441 tgacttcagc acgcgtcttg taggtcccgt catctttgaa agatatagtg cgttcctgta
     4501 cataaccttc aggcatggca ctcttgaaaa agtcatgacg tttcatgtga tccggataac
     4561 gggaaaagca ttgaacacca taggtcagag tagtgacaag tgttggccac ggaacaggta
     4621 gttttccagt agtgcaaata aatttaaggg tgagttttcc gtttgtagca tcaccttcac
     4681 cctctccacg gacagaaaat ttgtgcccat taacatcacc atctaattca acaagaattg
     4741 ggacaactcc agtgaaaagt tcttctcctt tgctcataaa taatcatcct cctaaggtac
     4801 ccggggatcc gatgcatctt gctcgataca tttgcccgat aatatattga tatctaatct
     4861 ttatttatta tagatatgtt agtcataatt ttgcattaaa gcacgttaat tataactaat
     4921 taaaaatgag aagtaaacaa aaaagtgctc ccaaattatt cattagacac agtgattgta
     4981 tttctgggtt agcatttggt ttagtcaaaa atatcaggct agcggatcct ctagagtcga
     5041 cctgcaggca tgcaagcttt taaaaagcaa atatgagcca aataaatata ttctaattct
     5101 acaaacaaaa atttgagcaa attcagtgtc gattttttaa gacactgccc agttacatgc
     5161 aaattaaaat tttcatgatt ttttatagtt cctaacaggg ttaaaatttg tataacgaaa
     5221 gtataatgtt tatataacgt tagtataata aagcatttta acattatact tttgataatc
     5281 gtttatcgtc gtcatcacaa taacttttaa aatactcgtg cataattcaa cagctgacct
     5341 cccaataact acatggtgtt atcgggaggt cagctgttag cacttatatt ttgttattgt
     5401 tcttcctcga tttcgtctat cattttgtga ttaatttctc ttttttcttg ttctgttaag
     5461 tcataaagtt cactagctaa atactctttt tgtttccaaa tataaaaaat ttgatagata
     5521 tattcggttg gatcaatttc ttttaagtaa tctaaatccc cattttttaa tttcttttta
     5581 gcctctttaa ataatcctga ataaactaat acctgtttac ctttaagtga tttataaaat
     5641 gcatcaaaga ctttttgatt tattaaataa tcactatctt taccagaata cttagccatt
     5701 tcatataatt ctttattatt attttgtctt attttttgaa cttgaacttg tgttatttct
     5761 gaaatgcccg ttacatcacg ccataaatct aaccattctt gttggctaat ataatatctt
     5821 ttatctgtga aatacgattt atttactgca attaacacat gaaaatgagg attataatca
     5881 tctctttttt tattatatgt aatctctaac ttacgaacat atccctttat aacactacct
     5941 actttttttc tctttataag ttttctaaaa gaattattat aacgttttat ttcattttct
     6001 aattcatcac tcattacatt aggtgtagtc aaagttaaaa agataaactc ctttttctct
     6061 tgctgcttaa tatattgcat catcaaagat aaacccaatg catcttttct agcttttctc
     6121 caagcacaga caggacaaaa tcgattttta caagaattag ctttatataa tttctgtttt
     6181 tctaaagttt tatcagctac aaaagacaga aatgtattgc aatcttcaac taaatccatt
     6241 tgattctctc caatatgacg tttaataaat ttctgaaata cttgatttct ttgttttttc
     6301 tcagtatact tttccatgtt ataacacata aaaacaactt agttttcaca aactatgaca
     6361 ataaaaaaag ttgctttttc ccctttctat gtatgttttt tactagtcat ttaaaacgat
     6421 acattaatag gtacgaaaaa gcaacttttt ttgcgcttaa aaccagtcat accaataact
     6481 taagggtaac tagcctcgcc ggcaatagtt acccttatta tcaagataag aaagaaaagg
     6541 atttttcgct acgctcaaat ccttt
//
