LOCUS       pJB94                   8780 bp ds-DNA     circular     07-SEP-2023
DEFINITION  .
COMMENT     LOCUS       pMTL84151               6297 bp    DNA     circular
            DEFINITION  Shuttle vector pMTL84151. clostron.com REFERENCE   1
            (bases 1 to 6297)   AUTHORS   Heap,J.T., Pennington,O.J.,
            Cartman,S.T. and Minton,N.P.   TITLE     A modular system for
            Clostridium shuttle plasmids   JOURNAL   Journal of Microbiological
            Methods (2009) 78(1) 79-85    PUBMED   19445976
FEATURES             Location/Qualifiers
     terminator      9..59
                     /label="CD0164 terminator"
     primer_bind     63..80
                     /label="M13R"
     CDS             400..1821
                     /label="SacB"
                     /note="gene: Bacillus subtilis sacB product: secreted levansucrase that renders bacterial growth sensitive to sucrose note: negative selection marker translation: MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISHITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYHIVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEWSGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDGKTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKAYYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDEIERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLKMDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSVVKDSILEQGQLTVNK"
     CDS             400..1821
                     /label="SacB"
                     /note="gene: Bacillus subtilis sacB product: secreted levansucrase that renders bacterial growth sensitive to sucrose note: negative selection marker translation: MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISHITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYHIVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEWSGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDGKTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKAYYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDEIERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLKMDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSVVKDSILEQGQLTVNK"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISHITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYHIVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEWSGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDGKTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKAYYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDEIERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLKMDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSVVKDSILEQGQLTVNK*"
     CDS             1822..2013
                     /label="lacZ alpha"
     primer_bind     complement(1851..1868)
                     /label="M13F"
     terminator      2015..2056
                     /label="Cpa fdx terminator"
     CDS             2221..2868
                     /label="orfB"
     misc_feature    complement(2912..2940)
                     /label="riboswitch piece A 3'"
     misc_feature    complement(3129..3186)
                     /label="Riboswitch"
     pCD6ori         3187..4553
                     /label="repA"
     pCD6ori         4554..4821
                     /label="repA"
     CDS             5637..6260
                     /label="catP"
     rep_origin      6473..7018
                     /label="ColE1 RNA II"
     misc_feature    complement(7286..7977)
                     /label="oriTtn916"
     CDS             8206..8646
                     /label="TraJ"
ORIGIN
        1 cctgcaggat aaaaaaattg tagataaatt ttataaaata gttttatcta caattttttt
       61 atcaggaaac agctatgacc gcggccgcgt cgactagttc tttaggcccg tagtctgcaa
      121 atccttttat gattttctat caaacaaaag aggaaaatag accagttgca atccaaacga
      181 gagtctaata gaatgaggtc gaaaagtaaa tcgcgcgggt ttgttactga taaagcaggc
      241 aagacctaaa atgtgtaaag ggcaaagtgt atactttggc gtcacccctt acatatttta
      301 ggtctttttt tattgtgcgt aactaacttg ccatcttcaa acaggagggc tggaagaagc
      361 agaccgctaa cacagtacat aaaaaaggag acatgaacga tgaacatcaa aaagtttgca
      421 aaacaagcaa cagtattaac ctttactacc gcactgctgg caggaggcgc aactcaagcg
      481 tttgcgaaag aaacgaacca aaagccatat aaggaaacat acggcatttc ccatattaca
      541 cgccatgata tgctgcaaat ccctgaacag caaaaaaatg aaaaatatca agttcctgaa
      601 tttgattcgt ccacaattaa aaatatctct tctgcaaaag gcctggacgt ttgggacagc
      661 tggccattac aaaacgctga cggcactgtc gcaaactatc acggctacca catcgtcttt
      721 gcattagccg gagatcctaa aaatgcggat gacacatcga tttacatgtt ctatcaaaaa
      781 gtcggcgaaa cttctattga cagctggaaa aacgctggcc gcgtctttaa agacagcgac
      841 aaattcgatg caaatgattc tatcctaaaa gaccaaacac aagaatggtc aggttcagcc
      901 acatttacat ctgacggaaa aatccgttta ttctacactg atttctccgg taaacattac
      961 ggcaaacaaa cactgacaac tgcacaagtt aacgtatcag catcagacag ctctttgaac
     1021 atcaacggtg tagaggatta taaatcaatc tttgacggtg acggaaaaac gtatcaaaat
     1081 gtacagcagt tcatcgatga aggcaactac agctcaggcg acaaccatac gctgagagat
     1141 cctcactacg tagaagataa aggccacaaa tacttagtat ttgaagcaaa cactggaact
     1201 gaagatggct accaaggcga agaatcttta tttaacaaag catactatgg caaaagcaca
     1261 tcattcttcc gtcaagaaag tcaaaaactt ctgcaaagcg ataaaaaacg cacggctgag
     1321 ttagcaaacg gcgctctcgg tatgattgag ctaaacgatg attacacact gaaaaaagtg
     1381 atgaaaccgc tgattgcatc taacacagta acagatgaaa ttgaacgcgc gaacgtcttt
     1441 aaaatgaacg gcaaatggta cctgttcact gactcccgcg gatcaaaaat gacgattgac
     1501 ggcattacgt ctaacgatat ttacatgctt ggttatgttt ctaattcttt aactggccca
     1561 tacaagccgc tgaacaaaac tggccttgtg ttaaaaatgg atcttgatcc taacgatgta
     1621 acctttactt actcacactt cgctgtacct caagcgaaag gaaacaatgt cgtgattaca
     1681 agctatatga caaacagagg attctacgca gacaaacaat caacgtttgc gcctagcttc
     1741 ctgctgaaca tcaaaggcaa gaaaacatct gttgtcaaag acagcatcct tgaacaagga
     1801 caattaacag ttaacaaata actcgaggcc tgcagacatg caagcttggc actggccgtc
     1861 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     1921 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     1981 cagttgcgca gcctgaatgg cgaatggcgc tagcataaaa ataagaagcc tgcatttgca
     2041 ggcttcttat ttttatggcg cgcccgccct taagtctaaa aattagggga gatgtaagga
     2101 tttgggaaaa atagaagatg ttataatcat aaatatggta ttcgtaggct taaagtcaaa
     2161 aaggaggtga aatataaata gatttttagc taaattaagt aagaaatagg aggagattta
     2221 ttgaacaaaa aattagaaaa accatttgta tataagagag agtacgattt gactggatat
     2281 gatgttgaaa ttttacaaaa atatgagtta gaacaagcaa tatatgttta tgttgggagt
     2341 agttgtgcat ataacatgag agctagaagt agtaaatgga gataccatat aagaacaaat
     2401 aataagtcta tatgttgtaa cattaaaaat tttatacata acttggaatt gttttataaa
     2461 atggaattaa agttgtcaga taatattatt aatgataagc tatactatag caatatagca
     2521 gagtttgaag aatttgaaac actagaaaaa gctagagagg tagaaagtac tattataagt
     2581 caatatcaat ttttagattc tataaatcac atgttaaaac aaaaaataat tttattgagt
     2641 aataaggata gtgtgttaaa cataactaaa aatggaaata caaattattt gaaagtaaaa
     2701 aataaataca tagaaaaaca taagaacaag ccaataatga gataccatat caactgtcaa
     2761 ttcaatacag atggaagtgt caaaagtatt acacaggagt ttgaaccaat attggaatta
     2821 aacaaaaaaa ataccctaag ccgaccaagc agagtatttt taaaataata ttttaagata
     2881 acaacaaaat gagataatac tactagacaa tgacaactca actaccaatt gagtttatgg
     2941 agctaccaac tccaatatcg gtctaactga ttaagtatct gtagttatat aataatattg
     3001 ctatcaattt tagcatctta acaatattat tatacatact aagctaaaat tattcaatag
     3061 ttgtaaaagt tgattagtca ataagtatat atttaatgta gtgttatctc ttaaaaaaac
     3121 tagataagcG GTGATACCAG CATCGTCTTG ATGCCCTTGG CAGCACCCTG CTAAGGAGGC
     3181 AACAAGATGG AACAATTAGA TTCAAAATAT AAGTTGAAAA AATTTCTAAT GGCAGTATTT
     3241 AGAGATGGTA TAGGACAAGG AAATAATCTT ATTGATAATG AATATGTTAG AGTATTTCAA
     3301 AATAATAAAA GTAATAGTAA ACAATTAGAA CTCGGAGAAG AATTTAAAGA ATATAGTAAA
     3361 ACAACTTTTT TTAAAAATAT AGATGATATA GTAGAATTTA CCTTCGCAAA AAATATTTAT
     3421 TATGAAAATA CATTTTTTAA CCTATGTACA ACTGATGGAA AAGCAGGAAC CAATGAAAAC
     3481 TTAATAAATA GATATGCATT AGGATTTGAT TTTGACAAAA AAGAATTAGG ACAAGGTTTT
     3541 AATTATAAAG ATATAATTAA TTTATTTACT AAGATAGGAT TACATTATCA TATCCTAGTT
     3601 GATAGTGGAA ATGGATTCCA TGTTTATGTG CTAATTAATA AAACTAATAA CATTAAGTTA
     3661 GTATCAGAAG TTACAAATAC ATTAATAAAT AAATTGGGTG CAGATAAACA AGCAAATTTA
     3721 TCTACTCAAG TATTAAGAGT ACCTTATACA TATAATATTA AAAATACTAC TAAACAAGTA
     3781 AAAATAATAC ACCAAGACAA AAATATATAT AGATATGACA TAGAAAAGTT AGCTAAAAAA
     3841 TATTGCAAAG ATGTAAAAAC AGTAGGTAAT ACTAATACAA AATATATATT AGATAGTAAG
     3901 CTACCAAATT GTATAGTAGA TATTTTAAAA AATGGTAGTA AAGATGGACA TAAAAACCTA
     3961 GATTTGCAAA AAATAGTTGT GACTTTAAGA TTGAGGAATA AAAGTTTAAG TCAAGTAATA
     4021 TCCGTTGCTA GAGAATGGAA CTATATATCA CAAAATAGTC TTTCAAATAG TGAGCTAGAA
     4081 TATCAAGTCA AGTATATGTA TGAGAAGCTT AAAACGGTTA ATTTTGGTTG TACAGGTTGT
     4141 GAGTTTAATA GTGATTGTTG GAATAAAATA GAATCAGATT TTATATATAG TGATGAAGAT
     4201 ACTTTGTTCA ATATGCCACA TAAGCACTCA AAGGATTTGA AATATAAGAA TAGGAAAGGG
     4261 GTTAAAATAA TGACTGGTAA TCAATTGTTT ATCTATAATG TGTTACTTAA CAATAAAGAT
     4321 AGAGAATTAA ACATAGACGA TATAATGGAG CTGATAACCT ATAAACGTAA GAAGAAAGTT
     4381 AAAAACATTG TTATGAGTGA AAAGACATTA AGAGAAACAT TAAAAGAACT TCAACATAAT
     4441 GATTATATTA CAAAAACAAA AGGTGTTACA AAGCTAGGAA TAAAAGATAC ATACAATGTA
     4501 AAAGAAGTTA GATGTAATAT AGATAAACAA TATACTATTA GTTACTTTGT TACCATGGCA
     4561 GTAATTTGGG GAATAATTTC AACTGAAGAA TTAAGATTAT ATACTCATAT GAGATATAAG
     4621 CAAGATTTAT TGGTCAAAGA TGATAAAATA AAAGGAAATA TATTAAGAAT TAATCAAGAG
     4681 GAATTAGCAA AAGATTTAGG AGTAACACAG CAAAGAATTT CAAATATGAT AGAATCTTTA
     4741 TTAGATACTA AAATTTTAGA TGTATGGGAA ACTAAAATAA ATGATAGAGG ATTTATGTAC
     4801 TATACATATA GATTAAACAA GTAGATTTTT GATAGGATTA GAATTGATTT TCTAGTCCTA
     4861 TTTTTATGCA AAAAAACTAA TTAATAAAAA TTTCTTTTGG TAAAATAATT GTACGAGAAT
     4921 TGCAAAAAAA AAATGGCATC AAAGTATTGA AATTAAGCCG TTTTAAAAAT TTCTTTTGGT
     4981 AAAATAATTG TACATATATA TGTAGTATAT ATATATATGT TTTTTAGAGA ATGTATAACT
     5041 AGAATATAGA GCTAGAATAT AGAGAATGTA TAACTAGAAT ATAGAGCTAG AATATAGAGA
     5101 ATGTATAACT AGAATATAGA GCTAGAATAT AGAGAATGTA TAACTAGAAT ATAGAGCTAG
     5161 AATATAGAGA ATGTATAACT AGAATATAGA GCTAGAATAT AGAGAATGTA TAACTAGAAT
     5221 ATAGAGCTAG AATATAGAGA ATGTATAACT AGAATATAGA GCTAGAATCC TAATTAGTAG
     5281 GTGCTTTTTT AAAACAAGTT AAAAATCAAA AATAGTATTA GTAAGCATTG GAAATGCTAG
     5341 ATTCTAAAAT AGAAAAGTAA AAAATTGGTG CACTATCTAA ACTTATCTAT ATCGCTTTTT
     5401 CCGTCGTTTG GTTCTCTAGT TACGATACAG GGGATATGCT TATATTGAGT TATAGTACTA
     5461 ATCAGTGCTT AATATAGTTA ATAAAATTAT AGTTACCATA GTTTAGTAAC TATGATGTAT
     5521 GTTAGTTAGA AACTTGCATT TCggccggcc agtgggcaag ttgaaaaatt cacaaaaatg
     5581 tggtataata tctttgttca ttagagcgat aaacttgaat ttgagaggga acttagatgg
     5641 tatttgaaaa aattgataaa aatagttgga acagaaaaga gtattttgac cactactttg
     5701 caagtgtacc ttgtacctac agcatgaccg ttaaagtgga tatcacacaa ataaaggaaa
     5761 agggaatgaa actatatcct gcaatgcttt attatattgc aatgattgta aaccgccatt
     5821 cagagtttag gacggcaatc aatcaagatg gtgaattggg gatatatgat gagatgatac
     5881 caagctatac aatatttcac aatgatactg aaacattttc cagcctttgg actgagtgta
     5941 agtctgactt taaatcattt ttagcagatt atgaaagtga tacgcaacgg tatggaaaca
     6001 atcatagaat ggaaggaaag ccaaatgctc cggaaaacat ttttaatgta tctatgatac
     6061 cgtggtcaac cttcgatggc tttaatctga atttgcagaa aggatatgat tatttgattc
     6121 ctatttttac tatggggaaa tattataaag aagataacaa aattatactt cctttggcaa
     6181 ttcaagttca tcacgcagta tgtgacggat ttcacatttg ccgttttgta aacgaattgc
     6241 aggaattgat aaatagttaa cttcaggttt gtctgtaact aaaaacaagt atttaagcaa
     6301 aaacatcgta gaaatacggt gttttttgtt accctaagtt taaactcctt tttgataatc
     6361 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     6421 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6481 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6541 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     6601 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6661 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6721 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6781 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6841 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6901 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6961 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     7021 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     7081 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     7141 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     7201 cggaagagcg cccaatacgc agggccCtaa catcttctat ttttcccaaa tccttacatc
     7261 tcccctaatt tttagactta agggcgaatt cgccctcaaa ggacgaatat gtcgcctaaa
     7321 gcataatgct ctgaataaga atagaaacca tagtcctcat gaagaaaata ggacagtttc
     7381 agttgtaaga tgttttcgac cacctgctgt acgtctgttg tcggaaagcg aatccttaca
     7441 taatcaaaca gcatttcaag gggagcgtcg ggattgaagc gttccagagc ttcccaaagg
     7501 gactgctgta aatcctctga tggcttgact tttcctgttt caatatcgct tagatactgc
     7561 cttgtaatac cagtcgcaac agctaaacgg ttttgagata gtccataagc caagcgtttt
     7621 tcttttaaat gctgtaacca agtttgttca ttcagtaaaa atccctccaa tcaaaaaggc
     7681 cgtatgtcaa cttttaaagc ccatttgaca tacgctgaaa ttttgtaaat cccttgtaac
     7741 caaaggattt tctaatgttt ttttgactgt ttcctgtcga tttgtacccc cctgttagat
     7801 acggggggtt aagtgctggc gtggctattg ccacaccagc cagcaagatc agtccacacc
     7861 tgcgacttcc gcttcgcacg tcgcctgcgt ggactgtctg ctgttggata actttttaat
     7921 ttcctccaag aaatcatatc cttttggtac aaggggagta taaaaaaggg cgaattctgc
     7981 agattaccta ataatttatc tacattccct ttagcctgct tcggggtcat tatagcgatt
     8041 ttttcggtat atccatcctt tttcgcacga tatacaggat tttgccaaag ggttcgtgta
     8101 gactttcctt ggtgtatcca acggcgtcag ccgggcagga taggtgaagt aggcccaccc
     8161 gcgagcgggt gttccttctt cactgtccct tattcgcacc tggcggtgct caacgggaat
     8221 cctgctctgc gaggctggcc ggctaccgcc ggcgtaacag atgagggcaa gcggatggct
     8281 gatgaaacca agccaaccag gaagggcagc ccacctatca aggtgtactg ccttccagac
     8341 gaacgaagag cgattgagga aaaggcggcg gcggccggca tgagcctgtc ggcctacctg
     8401 ctggccgtcg gccagggcta caaaatcacg ggcgtcgtgg actatgagca cgtccgcgag
     8461 ctggcccgca tcaatggcga cctgggccgc ctgggcggcc tgctgaaact ctggctcacc
     8521 gacgacccgc gcacggcgcg gttcggtgat gccacgatcc tcgccctgct ggcgaagatc
     8581 gaagagaagc aggacgagct tggcaaggtc atgatgggcg tggtccgccc gagggcagag
     8641 ccatgacttt tttagccgct aaaacggccg gggggtgcgc gtgattgcca agcacgtccc
     8701 catgcgctcc atcaagaaga gcgacttcgc ggagctggtg aagtacatca ccgacgagca
     8761 aggcaagacc gatcGGGCCc
//