LOCUS       p066_pAAV-C2m2-mKate    5852 bp ds-DNA     circular     08-SEP-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    858..876
                     /label="tet operator"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    894..912
                     /label="tet operator"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    984..992
                     /label="Kozak"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    993..1056
                     /label="Signal peptide"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    1074..1550
                     /label="C2m2"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1557..1637
                     /label="linker"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             1638..2333
                     /label="mKate"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    2341..2929
                     /label="WPRE"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    3506..3627
                     /label="SV40poly(A)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    3730..3859
                     /label="ITR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             4313..4972
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     rep_origin      5070..5752
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
ORIGIN
        1 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc
       61 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag agagggagtg
      121 gccaactcca tcactagggg ttcctactag taacatatcc tggtgtggag taggggacgc
      181 tgctctgaca gaggctcggg ggcctgagct ggctctgtga gctggggagg aggcagacag
      241 ccaggccttg tctgcaagca gacctggcag cattgggctg gccgcccccc agggcctcct
      301 cttcatgccc agtgaatgac tcaccttggc acagacacaa tgttcggggt gggcacagtg
      361 cctgcttccc gccgcacccc agcccccctc aaatgccttc cgagaagccc attgagcagg
      421 gggcttgcat tgcaccccag cctgacagcc tggcatcttg ggataaaagc agcacagccc
      481 cctaggggct gcccttgctg tgtggcgcca ccggcggtgg agaacaaggc tctattcagc
      541 ctgtgcccag gaaaggggat caggggatgc ccaggcatgg acagtgggtg gcaggggggg
      601 agaggagggc tgtctgcttc ccagaagtcc aaggacacaa atgggtgagg ggagagctct
      661 ccccatagct gggctgcggc ccaaccccac cccctcaggc tatgccaggg ggtgttgcca
      721 ggggcacccg ggcatcgcca gtctagccca ctccttcata aagccctcgt atcccaggag
      781 cgagcagagc cagagcaggt tggagaggag acgcatcacc tctgctgctt gcctaaaaca
      841 ggtaagtccc attaatctcc ctatcagtga tagagaaggt ctgaagagtt tactccctat
      901 cagtgataga gattaattcc tctactaacc ttgttcatct tttctttttt tttctacagg
      961 tcctgggtga ttaacagctt aagGCCGCCA CCATGCAGGT CTCCCGTGTG Ctggccgcgc
     1021 tgtgcggcat gctactctgc gcctctggcc tcttcgccgc gtctggtgac catatgcacg
     1081 gatgttctga gcccctgggc ctgaagaata acacaattcc tgacagccag atgtcagcct
     1141 ccagcagcta caatacatgg aacctgcgtg cttttggctg gtacccccac ttgggaaggc
     1201 tggataatca gggcaatatc aatgcctgga cggctcagag caacagtgcc aaggaatggc
     1261 tgcaggttga cctgggcact cagaggcaag tgacaggaat catcacccag ggggcccgtg
     1321 actttggcca catccagtat gtggcgtcct acaaggtagc ccacagtgat gatggtgtgc
     1381 agtggactgt atatgaggag caaggaagca gcaaggtctt ccagggcaac ttggacaaca
     1441 actcccacaa gaagaacatc ttcgagaaac ccttcatggc tcgctacgtg cgtgtccttc
     1501 cagtgtcctg gcataaccgc atcaccctgc gcctggagct gctgggctgt aagcttGGCA
     1561 CTGGAGGCTC TGGAGGCACT GGAGGCTCTG GAGGCACCGG TAGCACTAGC GGCGGAAGCG
     1621 GCGGGACAGG TACGCGTgtg agcgagctga ttaaggagaa catgcacatg aagctgtaca
     1681 tggagggcac cgtgaacaac caccacttca agtgcacatc cgagggcgaa ggcaagccct
     1741 acgagggcac ccagaccatg agaatcaagg tcgtcgaggg cggccctctc cccttcgcct
     1801 tcgacatcct ggctaccagc ttcatgtacg gcagcaaaac cttcatcaac caccctcagg
     1861 gcatccccga cttctttaag cagtccttcc ctgagggctt cacatgggag agagtcacca
     1921 catacgaaga cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc
     1981 tcatctacaa cgtcaagatt agaggggtga acttcccagc caacggccct gtgatgcaga
     2041 agaaaacact cggctgggag gcctccaccg agacgctgta ccccgctgac ggcggcctgg
     2101 aaggcgcatg tgacatggcc ctgaagctcg tgggcggggg ccacctgatc tgcaacttgg
     2161 agaccacata cagatccaag aaacccgcta agaacctcaa gatgcccggc gtctacaacg
     2221 tggacaggag actggaaaga atcaaggagg ccgacaatga gacctacgtc gagcagcacg
     2281 aggtggctgt ggccagatac tctactggtg gcgctggtga tggaggtaaa tgaggatcct
     2341 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     2401 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     2461 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     2521 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     2581 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     2641 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     2701 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     2761 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     2821 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     2881 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgaattctgc
     2941 atgtttaaac atacatactt ctttacattc cagatatctg catcaaacac cattgtcaca
     3001 ctccatcgcg atcacgcgag ccgaacgaac aaacccaaca acatgaaact acctagcgct
     3061 tctattcagc atgatgtctt tcgtatacac aaattcggtt ctacagggta acctaggctg
     3121 catgtttaaa catacatact tctttacatt ccagatatct gcatcaaaca ccattgtcac
     3181 actccatcgc gatcacgcga gccgaacgaa caaacccaac aacatgaaac tacctagcgc
     3241 ttctattcag catgatgtct ttcgtataca caaattcggt tctacagggt aacctaggct
     3301 gcatgtttaa acatacatac ttctttacat tccagatatc tgcatcaaac accattgtca
     3361 cactccatcg cgatcacgcg agccgaacga acaaacccaa caacatgaaa ctacctagcg
     3421 cttctattca gcatgatgtc tttcgtatac acaaattcgg ttctacaggg taacctaggc
     3481 tgcaggtacc ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact
     3541 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     3601 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     3661 gttcaggggg agatgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa
     3721 atcaagctta ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc
     3781 tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag
     3841 tgagcgagcg agcgcgcaga gagggagtgg ccaagctagc gggcgattaa ggaaagggct
     3901 agatcattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     3961 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     4021 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     4081 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     4141 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     4201 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     4261 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     4321 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact
     4381 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     4441 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     4501 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     4561 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     4621 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     4681 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     4741 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     4801 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     4861 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     4921 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     4981 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     5041 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     5101 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     5161 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     5221 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     5281 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     5341 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     5401 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     5461 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     5521 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     5581 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     5641 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     5701 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     5761 gttcctggcc ttttgctggc cttttgctca catgtaataa acacacacac accaacaacc
     5821 gtggttggtt gttgtgttgg tttattctcg ag
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