LOCUS       p063_pAAV-C2-SNAP       5699 bp ds-DNA     circular     08-SEP-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    152..833
                     /label="GFAP promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    632..653
                     /label="dalis"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    808..813
                     /label="reikia cia mutacijos"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    812..812
                     /label="13C>T R5C"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    813..813
                     /label="14G>A R5H"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    823..833
                     /label="pabaiga"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    993..1073
                     /label="Signal peptide"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    1060..1073
                     /label="non-specific aa"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1074..1556
                     /label="c2"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1557..1637
                     /label="linker"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1638..2180
                     /label="SNAP-tag"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2188..2776
                     /label="WPRE"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    3353..3474
                     /label="SV40polyA"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    3577..3706
                     /label="ITR"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             4160..4819
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     rep_origin      4917..5599
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
ORIGIN
        1 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc
       61 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag agagggagtg
      121 gccaactcca tcactagggg ttcctactag taacatatcc tggtgtggag taggggacgc
      181 tgctctgaca gaggctcggg ggcctgagct ggctctgtga gctggggagg aggcagacag
      241 ccaggccttg tctgcaagca gacctggcag cattgggctg gccgcccccc agggcctcct
      301 cttcatgccc agtgaatgac tcaccttggc acagacacaa tgttcggggt gggcacagtg
      361 cctgcttccc gccgcacccc agcccccctc aaatgccttc cgagaagccc attgagcagg
      421 gggcttgcat tgcaccccag cctgacagcc tggcatcttg ggataaaagc agcacagccc
      481 cctaggggct gcccttgctg tgtggcgcca ccggcggtgg agaacaaggc tctattcagc
      541 ctgtgcccag gaaaggggat caggggatgc ccaggcatgg acagtgggtg gcaggggggg
      601 agaggagggc tgtctgcttc ccagaagtcc aaggacacaa atgggtgagg ggagagctct
      661 ccccatagct gggctgcggc ccaaccccac cccctcaggc tatgccaggg ggtgttgcca
      721 ggggcacccg ggcatcgcca gtctagccca ctccttcata aagccctcgt atcccaggag
      781 cgagcagagc cagagcaggt tggagaggag acgcatcacc tctgctgctt gcctaaaaca
      841 ggtaagtccc attaatctcc ctatcagtga tagagaaggt ctgaagagtt tactccctat
      901 cagtgataga gattaattcc tctactaacc ttgttcatct tttctttttt tttctacagg
      961 tcctgggtga ttaacagctt aagGCCGCCA CCATGCAGGT CTCCCGTGTG Ctggccgcgc
     1021 tgtgcggcat gctactctgc gcctctggcc tcttcgccgc gtctggtgac catatgcacg
     1081 gatgttctga gcccctgggc ctgaagaata acacaattcc tgacagccag atgtcagcct
     1141 ccagcagcta caagacatgg aacctgcgtg cttttggctg gtacccccac ttgggaaggc
     1201 tggataatca gggcaagatc aatgcctgga cggctcagag caacagtgcc aaggaatggc
     1261 tgcaggttga cctgggcact cagaggcaag tgacaggaat catcacccag ggggcccgtg
     1321 actttggcca catccagtat gtggcgtcct acaaggtagc ccacagtgat gatggtgtgc
     1381 agtggactgt atatgaggag caaggaagca gcaaggtctt ccagggcaac ttggacaaca
     1441 actcccacaa gaagaacatc ttcgagaaac ccttcatggc tcgctacgtg cgtgtccttc
     1501 cagtgtcctg gcataaccgc atcaccctgc gcctggagct gctgggctgt aagcttGGCA
     1561 CTGGAGGCTC TGGAGGCACT GGAGGCTCTG GAGGCACCGG TAGCACTAGC GGCGGAAGCG
     1621 GCGGGACAGG TACGCGTGAC AAAGACTGCG AAATGAAGCG CACCACCCTG GATAGCCCTC
     1681 TGGGCAAGCT GGAACTGTCT GGGTGCGAAC AGGGCCTGCA CCGTATCATC TTCCTGGGCA
     1741 AAGGAACATC TGCCGCCGAC GCCGTGGAAG TGCCTGCCCC AGCCGCCGTG CTGGGCGGAC
     1801 CAGAGCCACT GATGCAGGCC ACCGCCTGGC TCAACGCCTA CTTTCACCAG CCTGAGGCCA
     1861 TCGAGGAGTT CCCTGTGCCA GCCCTGCACC ACCCAGTGTT CCAGCAGGAG AGCTTTACCC
     1921 GCCAGGTGCT GTGGAAACTG CTGAAAGTGG TGAAGTTCGG AGAGGTCATC AGCTACAGCC
     1981 ACCTGGCCGC CCTGGCCGGC AATCCCGCCG CCACCGCCGC CGTGAAAACC GCCCTGAGCG
     2041 GAAATCCCGT GCCCATTCTG ATCCCCTGCC ACCGGGTGGT GCAGGGCGAC CTGGACGTGG
     2101 GGGGCTACGA GGGCGGGCTC GCCGTGAAAG AGTGGCTGCT GGCCCACGAG GGCCACAGAC
     2161 TGGGCAAGCC TGGGCTGGGT ggatcctaat caacctctgg attacaaaat ttgtgaaaga
     2221 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     2281 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     2341 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     2401 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     2461 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     2521 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     2581 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     2641 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     2701 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     2761 tgggccgcct ccccgcctga attctgcatg tttaaacata catacttctt tacattccag
     2821 atatctgcat caaacaccat tgtcacactc catcgcgatc acgcgagccg aacgaacaaa
     2881 cccaacaaca tgaaactacc tagcgcttct attcagcatg atgtctttcg tatacacaaa
     2941 ttcggttcta cagggtaacc taggctgcat gtttaaacat acatacttct ttacattcca
     3001 gatatctgca tcaaacacca ttgtcacact ccatcgcgat cacgcgagcc gaacgaacaa
     3061 acccaacaac atgaaactac ctagcgcttc tattcagcat gatgtctttc gtatacacaa
     3121 attcggttct acagggtaac ctaggctgca tgtttaaaca tacatacttc tttacattcc
     3181 agatatctgc atcaaacacc attgtcacac tccatcgcga tcacgcgagc cgaacgaaca
     3241 aacccaacaa catgaaacta cctagcgctt ctattcagca tgatgtcttt cgtatacaca
     3301 aattcggttc tacagggtaa cctaggctgc aggtaccttc gagcagacat gataagatac
     3361 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     3421 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     3481 aacaattgca ttcattttat gtttcaggtt cagggggaga tgtgggaggt tttttaaagc
     3541 aagtaaaacc tctacaaatg tggtaaaatc aagcttagga acccctagtg atggagttgg
     3601 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
     3661 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
     3721 agctagcggg cgattaagga aagggctaga tcattcttga agacgaaagg gcctcgtgat
     3781 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     3841 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     3901 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     3961 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     4021 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     4081 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     4141 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     4201 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     4261 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     4321 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     4381 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     4441 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     4501 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     4561 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     4621 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     4681 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     4741 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     4801 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     4861 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     4921 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     4981 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     5041 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     5101 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     5161 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     5221 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     5281 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     5341 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     5401 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     5461 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     5521 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     5581 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     5641 gtaataaaca cacacacacc aacaaccgtg gttggttgtt gtgttggttt attctcgag
//