LOCUS       pcft3-2ilov-1        3231 bp DNA     circular SYN 13-SEP-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pCFT3.2/iLOV
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 13, 2023 from SnapGene Viewer 7.0.2
            https://www.snapgene.com
COMMENT     Imported using the Genbank importer. File name: pudo4a.gb
FEATURES             Location/Qualifiers
     source          1..3231
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     rep_origin      1..770
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
                     /note="color: #ffff00"
     primer_bind     596..613
                     /label=SeqbGO1 (bG051)
                     /note="note: sequence: GTACGCAACAGCTCATTG ApEinfo_revcolor:
                     #c6c9d1"
                     /note="color: black; sequence: GTACGCAACAGCTCATTG"
     primer_bind     624..638
                     /label=SeqbGO1 (bG051)
                     /note="note: sequence: GTACGCAACAGCTCATTG ApEinfo_revcolor:
                     #c6c9d1"
                     /note="color: black; sequence: GTACGCAACAGCTCATTG"
     CDS             771..1433
                     /codon_start=1
                     /product="replication protein for the broad-host-range
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /note="color: #993366"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     1434..1454
                     /label=M13-rev
                     /note="ApEinfo_revcolor: #ff9ccd"
                     /note="color: #ff9ccd; direction: RIGHT"
     terminator      1472..1529
                     /label=thrLABC_ter
                     /note="ApEinfo_revcolor: #b1ff67"
                     /note="color: #b1ff67"
     promoter        1561..1579
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    1591..1607
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     misc_feature    1624..1671
                     /label=contains_RBS
                     /note="color: #c0c0c0"
     CDS             1672..2010
                     /label=iLOV
                     /note="color: #ff9ccd; direction: RIGHT"
     terminator      2088..2148
                     /label=L3S2P21_ter
                     /note="ApEinfo_revcolor: #b1ff67"
                     /note="color: #b1ff67"
     primer_bind     complement(2157..2174)
                     /label=M13-fwd
                     /note="ApEinfo_revcolor: #ff9ccd"
                     /note="color: #ff9ccd; direction: LEFT"
     primer_bind     complement(2157..2173)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     promoter        2173..2244
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             2245..3105
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 2245 .. 2313 / #ccffcc / signal sequence
                        2: 2314 .. 3105 / #ccffcc
                     Cleavage site after base 2313"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        3106..3124
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     terminator      3145..3198
                     /label=arcA_ter
                     /note="ApEinfo_revcolor: #b1ff67"
                     /note="color: #b1ff67"
     promoter        complement(3213..3231)
                     /label=T3 promoter
                     /note="ApEinfo_revcolor: #ff9ccd"
                     /note="color: #ff9ccd; direction: LEFT"
ORIGIN
        1 gcggccaccg gctggctcgc ttcgctcggc ccgtggacaa ccctgctgga caagctgatg
       61 gacaggctgc gcctgcccac gagcttgacc acagggattg cccaccggct acccagcctt
      121 cgaccacata cccaccggct ccaactgcgc ggcctgcggc cttgccccat caattttttt
      181 aattttctct ggggaaaagc ctccggcctg cggcctgcgc gcttcgcttg ccggttggac
      241 accaagtgga aggcgggtca aggctcgcgc agcgaccgcg cagcggcttg gccttgacgc
      301 gcctggaacg acccaagcct atgcgagtgg gggcagtcga aggcgaagcc cgcccgcctg
      361 ccccccgagc ctcacggcgg cgagtgcggg ggttccaagg gggcagcgcc accttgggca
      421 aggccgaagg ccgcgcagtc gatcaacaag ccccggaggg gccacttttt gccggagggg
      481 gagccgcgcc gaaggcgtgg gggaaccccg caggggtgcc cttctttggg caccaaagaa
      541 ctagatatag ggcgaaatgc gaaagactta aaaatcaaca acttaaaaaa ggggggtacg
      601 caacagctca ttgcggcacc ccccgcaata gctcattgcg taggttaaag aaaatctgta
      661 attgactgcc acttttacgc aacgcataat tgttgtcgcg ctgccgaaaa gttgcagctg
      721 attgcgcatg gtgccgcaac cgtgcggcac cctaccgcat ggagataagc atggccacgc
      781 agtccagaga aatcggcatt caagccaaga acaagcccgg tcactgggtg caaacggaac
      841 gcaaagcgca tgaggcgtgg gccgggctta ttgcgaggaa acccacggcg gcaatgctgc
      901 tgcatcacct cgtggcgcag atgggccacc agaacgccgt ggtggtcagc cagaagacac
      961 tttccaagct catcggacgt tctttgcgga cggtccaata cgcagtcaag gacttggtgg
     1021 ccgagcgctg gatctccgtc gtgaagctca acggccccgg caccgtgtcg gcctacgtgg
     1081 tcaatgaccg cgtggcgtgg ggccagcccc gcgaccagtt gcgcctgtcg gtgttcagtg
     1141 ccgccgtggt ggttgatcac gacgaccagg acgaatcgct gttggggcat ggcgacctgc
     1201 gccgcatccc gaccctgtat ccgggcgagc agcaactacc gaccggcccc ggcgaggagc
     1261 cgcccagcca gcccggcatt ccgggcatgg aaccagacct gccagccttg accgaaacgg
     1321 aggaatggga acggcgcggg cagcagcgcc tgccgatgcc cgatgagccg tgttttctgg
     1381 acgatggcga gccgttggag ccgccgacac gggtcacgct gccgcgccgg tagcaggaaa
     1441 cagctatgac catgcccggg taagtgacta gcggaaacac agaaaaaagc ccgcacctga
     1501 cagtgcgggc tttttttttc gaccaaaggc tccgaagagc cgagtgtctt cggtgaattg
     1561 taatacgact cactataggg cgaattgaca ttgtgagcgg ataacaatat aatgtgtgga
     1621 attcgagctc ggtacccggg gatcctctag atttaagaag gagatataca tatgaaaatc
     1681 gagaagaact tcgtgattac cgatccgcgt ttaccggaca atccgatcat ctttgccagt
     1741 gatgggtttc tggaactgac ggaatatagc cgtgaggaaa ttcttggtcg caatgctcgc
     1801 tttctgcaag gacctgaaac cgaccaggca actgtccaga aaattcggga tgcgattcgc
     1861 gatcaacgcg aaacgacagt tcagctgatc aactatacca aatccggcaa gaaattctgg
     1921 aacctgttac acctccaacc agtacgtgac cagaaaggcg aattgcagta cttcattggc
     1981 gttcagctgg atggttcgga tcatgtgtaa cggaatgtcc agacctgcag caatggccga
     2041 cacggtctct gagagaagac acgtcgctct tcgacgtaag taactgactc ggtaccaaat
     2101 tccagaaaag aggcctcccg aaaggggggc cttttttcgt tttggtccta agcttaactg
     2161 gccgtcgttt tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     2221 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     2281 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     2341 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     2401 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     2461 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     2521 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     2581 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     2641 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     2701 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     2761 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     2821 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     2881 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     2941 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     3001 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     3061 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaataata cgactcacta
     3121 tagggtaact aattgaggcg cgcctaccac cgtcaaaaaa aacggcgctt tttagcgccg
     3181 tttttatttt tcaaccttcc taggtaagtg accctttagt gagggttaat t
//
