LOCUS       pCFT7.2/FAST            3270 bp ds-DNA     circular     11-SEP-2023
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name: pudo4a.gb
FEATURES             Location/Qualifiers
     primer          596..613
                     /label="SeqbGO1 (bG051)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: sequence: GTACGCAACAGCTCATTG ApEinfo_revcolor: #c6c9d1"
     primer_bind     1434..1454
                     /label="M13-rev"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="ApEinfo_revcolor: #ff9ccd"
     terminator      1472..1529
                     /label="thrLABC_ter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="ApEinfo_revcolor: #b1ff67"
     primer          complement(1534..1553)
                     /label="part2-amorce-igem-rev"
                     /note="sequence: CCGAAGACACTCGGCTCTTCTTGACAGCTAGCTCAGTC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1624..1671
                     /label="contains_RBS"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             1672..2049
                     /label="FAST"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     terminator      2127..2187
                     /label="L3S2P21_ter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="ApEinfo_revcolor: #b1ff67"
     primer_bind     complement(2196..2213)
                     /label="M13-fwd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="ApEinfo_revcolor: #ff9ccd"
     misc_feature    2214..2242
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="ApEinfo_revcolor: #c7b0e3"
     CDS             2284..3141
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="ApEinfo_revcolor: #ffef86"
     primer_bind     3145..3164
                     /label="T7"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="ApEinfo_revcolor: #ff9ccd"
     terminator      3184..3237
                     /label="arcA_ter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="ApEinfo_revcolor: #b1ff67"
     promoter        complement(3252..3270)
                     /label="T3 promoter"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="ApEinfo_revcolor: #ff9ccd"
ORIGIN
        1 GCGGCCACCG GCTGGCTCGC TTCGCTCGGC CCGTGGACAA CCCTGCTGGA CAAGCTGATG
       61 GACAGGCTGC GCCTGCCCAC GAGCTTGACC ACAGGGATTG CCCACCGGCT ACCCAGCCTT
      121 CGACCACATA CCCACCGGCT CCAACTGCGC GGCCTGCGGC CTTGCCCCAT CAATTTTTTT
      181 AATTTTCTCT GGGGAAAAGC CTCCGGCCTG CGGCCTGCGC GCTTCGCTTG CCGGTTGGAC
      241 ACCAAGTGGA AGGCGGGTCA AGGCTCGCGC AGCGACCGCG CAGCGGCTTG GCCTTGACGC
      301 GCCTGGAACG ACCCAAGCCT ATGCGAGTGG GGGCAGTCGA AGGCGAAGCC CGCCCGCCTG
      361 CCCCCCGAGC CTCACGGCGG CGAGTGCGGG GGTTCCAAGG GGGCAGCGCC ACCTTGGGCA
      421 AGGCCGAAGG CCGCGCAGTC GATCAACAAG CCCCGGAGGG GCCACTTTTT GCCGGAGGGG
      481 GAGCCGCGCC GAAGGCGTGG GGGAACCCCG CAGGGGTGCC CTTCTTTGGG CACCAAAGAA
      541 CTAGATATAG GGCGAAATGC GAAAGACTTA AAAATCAACA ACTTAAAAAA GGGGGGTACG
      601 CAACAGCTCA TTGCGGCACC CCCCGCAATA GCTCATTGCG TAGGTTAAAG AAAATCTGTA
      661 ATTGACTGCC ACTTTTACGC AACGCATAAT TGTTGTCGCG CTGCCGAAAA GTTGCAGCTG
      721 ATTGCGCATG GTGCCGCAAC CGTGCGGCAC CCTACCGCAT GGAGATAAGC ATGGCCACGC
      781 AGTCCAGAGA AATCGGCATT CAAGCCAAGA ACAAGCCCGG TCACTGGGTG CAAACGGAAC
      841 GCAAAGCGCA TGAGGCGTGG GCCGGGCTTA TTGCGAGGAA ACCCACGGCG GCAATGCTGC
      901 TGCATCACCT CGTGGCGCAG ATGGGCCACC AGAACGCCGT GGTGGTCAGC CAGAAGACAC
      961 TTTCCAAGCT CATCGGACGT TCTTTGCGGA CGGTCCAATA CGCAGTCAAG GACTTGGTGG
     1021 CCGAGCGCTG GATCTCCGTC GTGAAGCTCA ACGGCCCCGG CACCGTGTCG GCCTACGTGG
     1081 TCAATGACCG CGTGGCGTGG GGCCAGCCCC GCGACCAGTT GCGCCTGTCG GTGTTCAGTG
     1141 CCGCCGTGGT GGTTGATCAC GACGACCAGG ACGAATCGCT GTTGGGGCAT GGCGACCTGC
     1201 GCCGCATCCC GACCCTGTAT CCGGGCGAGC AGCAACTACC GACCGGCCCC GGCGAGGAGC
     1261 CGCCCAGCCA GCCCGGCATT CCGGGCATGG AACCAGACCT GCCAGCCTTG ACCGAAACGG
     1321 AGGAATGGGA ACGGCGCGGG CAGCAGCGCC TGCCGATGCC CGATGAGCCG TGTTTTCTGG
     1381 ACGATGGCGA GCCGTTGGAG CCGCCGACAC GGGTCACGCT GCCGCGCCGG TAGCAGGAAA
     1441 CAGCTATGAC CATGCCCGGG TAAGTGACTA GCGGAAACAC AGAAAAAAGC CCGCACCTGA
     1501 CAGTGCGGGC TTTTTTTTTC GACCAAAGGC TCCGAAGAGC CGAGTGTCTT CGGtgaattg
     1561 taatacgact cactataggg cgaattgaca ttgtgagcgg ataacaatat aatgtgtgga
     1621 attcgagctc ggtacccggg gatcctctag atttaagaag gagatataca tatggaacat
     1681 gtggcgttcg ggtccgagga tatcgagaat acgctggcca agatggatga tggccagctg
     1741 gatggcctgg cgttcggcgc catccaactc gacggcgacg ggaatatcct ccagtataat
     1801 gcggcggaag gggatatcac gggccgcgat cccaaacagg tgatcggcaa gaatttcttc
     1861 aaggacgtcg cccccgggac cgattcgccg gagttctatg gcaagttcaa ggaaggggtg
     1921 gcctcgggga atctcaatac gatgttcgaa tggatgatcc cgacgtcgcg cggccccacg
     1981 aaagtgaagg tccatatgaa aaaggccctg tccggggatt cgtattgggt cttcgtgaag
     2041 cgcgtgtaac ggaatgtcca gacctgcagc aatggcCGAC ACGGTCTCTG AGAGAAGACA
     2101 CGTCGCTCTT CGACGTAAGT AACTGACTCG GTACCAAATT CCAGAAAAGA GGCCTCCCGA
     2161 AAGGGGGGCC TTTTTTCGTT TTGGTCCTAA GCTTAACTGG CCGTCGTTTT ACATTCAAAT
     2221 ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG
     2281 AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT
     2341 CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT
     2401 GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC
     2461 CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA
     2521 TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC
     2581 TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA
     2641 TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG
     2701 ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC
     2761 CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG
     2821 ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA
     2881 GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG
     2941 CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG
     3001 TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC
     3061 TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT
     3121 GCCTCACTGA TTAAGCATTG GTAATAATAC GACTCACTAT AGGGTAACTA ATTGAGGCGC
     3181 GCCTACCACC GTCAAAAAAA ACGGCGCTTT TTAGCGCCGT TTTTATTTTT CAACCTTCCT
     3241 AGGTAAGTGA CCCTTTAGTG AGGGTTAATT
//