LOCUS       Exported                8089 bp ds-DNA     circular SYN 13-SEP-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pXK-1648 pXL-BacII-CMV-mCherry-CMV-(ATAACG)GFP-sgT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8089)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 14, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8089
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             132..992
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2338..2647
                     /label=PB 5'LTR
     primer_bind     2706..2756
                     /label=YTL-168
     primer_bind     2749..2769
                     /label=YTL-166
     primer_bind     2781..2805
                     /label=ZCY-5512
     enhancer        2789..3092
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     2941..2961
                     /label=YTL-151
     promoter        3093..3296
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3191..3211
                     /label=ZCY-5514
     primer_bind     3205..3227
                     /label=ZCY-5515
     primer_bind     complement(3318..3333)
                     /label=Primer 3
     primer_bind     3322..3343
                     /label=ZCY-2681
     primer_bind     3324..3342
                     /label=ZCY-5353
     primer_bind     3329..3351
                     /label=ZCY-4632
     CDS             3330..4040
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     3330..3349
                     /label=ZCY-315 cherry-WPRE primer-F NheI
     primer_bind     3332..3342
                     /label=ACG-F without shift
     primer_bind     complement(3357..3386)
                     /label=CasRx mCherry pre-gRNA spacer 2
     primer_bind     complement(3362..3391)
                     /label=CasRx mCherry pre-gRNA spacer 4
     primer_bind     complement(3365..3386)
                     /label=CasRx mCherry gRNA spacer 2
     primer_bind     complement(3370..3391)
                     /label=CasRx mCherry gRNA spacer 4
     primer_bind     3418..3437
                     /label=ZCY-1286
     primer_bind     3485..3496
                     /label=ZCY-2681
     primer_bind     complement(3608..3637)
                     /label=CasRx mCherry pre-gRNA spacer 3
     primer_bind     complement(3614..3634)
                     /label=YTL-178
     primer_bind     complement(3616..3637)
                     /label=CasRx mCherry gRNA spacer 3
     primer_bind     complement(3625..3654)
                     /label=CasRx mCherry pre-gRNA spacer 1
     primer_bind     complement(3625..3654)
                     /label=Primer 4
     primer_bind     complement(3633..3654)
                     /label=CasRx mCherry gRNA spacer 1
     primer_bind     complement(3741..3761)
                     /label=ZCY-5517
     primer_bind     complement(3747..3770)
                     /label=ZCY-5516
     primer_bind     complement(3827..3846)
                     /label=ZCY-1287
     primer_bind     complement(4018..4042)
                     /label=ZCY-5354
     primer_bind     complement(4018..4037)
                     /label=ZCY-2682
     primer_bind     complement(4018..4037)
                     /label=ZCY-4633
     primer_bind     complement(4019..4040)
                     /label=ZCY-5513
     misc_feature    4041..4628
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4259..4279)
                     /label=YTL-177
     primer_bind     4649..4669
                     /label=YTL-192
     misc_feature    4670..4895
                     /label=BGH polyA
     primer_bind     complement(4723..4743)
                     /label=YTL-144
     primer_bind     complement(4860..4882)
                     /label=YTL-232 saCAS9 R
     primer_bind     4861..4877
                     /label=YTL-166
     primer_bind     complement(4883..4903)
                     /label=ZCY-316 Cherry -WPRE primer-R AscI
     primer_bind     4895..4916
                     /label=Primer 1
     primer_bind     complement(4898..4961)
                     /label=YTL-169
     primer_bind     complement(4898..4919)
                     /label=YTL-167
     primer_bind     4954..4973
                     /label=YTL-168
     primer_bind     4966..4986
                     /label=YTL-166
     primer_bind     4998..5022
                     /label=ZCY-5512
     enhancer        5006..5309
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     5158..5178
                     /label=YTL-151
     promoter        5310..5513
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5408..5428
                     /label=ZCY-5514
     primer_bind     5422..5444
                     /label=ZCY-5515
     misc_feature    5465..5484
                     /label=CMV-profor primer
     misc_feature    5490..5511
                     /label=PEGFP-N-5
                     /note="OyIoµA"
     primer_bind     5542..5586
                     /label=target site-GFP-F
     primer_bind     5542..5570
                     /label=ACG-F without shift
     primer_bind     5542..5568
                     /label=(ATAACG)-F without shift
     primer_bind     5542..5568
                     /label=(ATAACG)-F no shift
     misc_feature    5554..5573
                     /label=(ATAACG)sg-T
     primer_bind     5557..5577
                     /label=ZCY-315 cherry-WPRE primer-F NheI
     CDS             5560..6274
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     misc_feature    5560..5646
                     /label=GFPL
     misc_feature    5560..5575
                     /label=GFP5'.seqR
     primer_bind     5560..5571
                     /label=ZCY-2681
     primer_bind     5560..5570
                     /label=ZCY-5353
     CDS             5561..6274
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     5571..5586
                     /label=GFP-F(A-G)
     misc_feature    5603..5624
                     /label=PEGFP-N-3
                     /note="·½IoµA"
     misc_feature    5850..5867
                     /label=GFPin.seqF
     misc_feature    6211..6232
                     /label=PEGFP-C-5
     primer_bind     complement(6255..6275)
                     /label=ZCY-5354
     primer_bind     complement(6255..6274)
                     /label=ZCY-2682
     primer_bind     complement(6255..6274)
                     /label=ZCY-4633
     primer_bind     complement(6256..6275)
                     /label=ZCY-5513
     misc_feature    6320..6341
                     /label=PEGFP-C-3
     misc_feature    6322..6534
                     /label= SV40 ployA
     primer_bind     complement(6523..6540)
                     /label=GFP-R XhoI
     primer_bind     6540..6563
                     /label=U6-F EcoRI
     promoter        6547..6795
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    6796..6815
                     /label=(ATAACG)sgRNA
     primer_bind     6796..6810
                     /label=(ATAACG)-F without shift
     primer_bind     6796..6810
                     /label=(ATAACG)-F no shift
     primer_bind     6800..6812
                     /label=ACG-F without shift
     primer_bind     6802..6813
                     /label=ZCY-2681
     primer_bind     6802..6812
                     /label=ZCY-5353
     misc_RNA        6816..6891
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(6960..6981)
                     /label=U6-R BamHI
     misc_feature    7149..7383
                     
     primer_bind     complement(7471..7487)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      7632..8087
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagct cggaattaac cctcactaaa gggaacaaaa gctgggtacc tcgcgcgact
     2221 tggtttgcca ttctttagcg cgcgtcgcgt cacacagctt ggccacaatg tggtttttgt
     2281 caaacgaaga ttctatgacg tgtttaaagt ttaggtcgag taaagcgcaa atctttttta
     2341 accctagaaa gatagtctgc gtaaaattga cgcatgcatt cttgaaatat tgctctctct
     2401 ttctaaatag cgcgaatccg tcgctgtgca tttaggacat ctcagtcgcc gcttggagct
     2461 cccgtgaggc gtgcttgtca atgcggtaag tgtcactgat tttgaactat aacgaccgcg
     2521 tgagtcaaaa tgacgcatga ttatctttta cgtgactttt aagatttaac tcatacgata
     2581 attatattgt tatttcatgt tctacttacg tgataactta ttatatatat attttcttgt
     2641 tatagatatc ccggaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
     2701 gcgatgtacg ggccagatat aggtacccat taattaaata gtaatcaatt acggggtcat
     2761 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     2821 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     2881 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     2941 tggcagtaca tcaagtgtat catatgccaa gtccgccccc tattgacgtc aatgacggta
     3001 aatggcccgc ctggcattat gcccagtaca tgaccttacg ggactttcct acttggcagt
     3061 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacaccaatg
     3121 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     3181 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaat aaccccgccc
     3241 cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agaggtcgtt
     3301 tagtgaaccg tcagatcacg cgtgccacca tggtgagcaa gggcgaggag gataacatgg
     3361 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
     3421 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     3481 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
     3541 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
     3601 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
     3661 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
     3721 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     3781 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga
     3841 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc
     3901 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg
     3961 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca
     4021 tggacgagct gtacaagtaa aatcaacctc tggattacaa aatttgtgaa agattgactg
     4081 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     4141 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     4201 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     4261 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     4321 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     4381 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     4441 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     4501 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     4561 tgcggcctct tccgcgtctc cgccttcgcc ctcagacgag tcggatctcc ctttggccgc
     4621 ctccccgcca ccggttaggg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     4681 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     4741 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     4801 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     4861 agcaggcatg ctggggatgc ggtgggctct atggcggcgc gcctaaaagt tttgttactg
     4921 acattgatta ttgactagta agctttagtt attaatagta atcaattacg gggtcattag
     4981 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     5041 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     5101 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     5161 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     5221 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     5281 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     5341 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     5401 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     5461 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
     5521 tgaaccgtca gatccgctag cgctaccggt cgggataacg gtgagcaagg gcgaggagct
     5581 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     5641 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     5701 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     5761 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     5821 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     5881 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     5941 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     6001 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     6061 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     6121 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     6181 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     6241 cgggatcact ctcggcatgg acgagctgta caagtccgga ctcagatcca ccggatctag
     6301 ataactgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     6361 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     6421 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     6481 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttactcgag
     6541 gaattcgagg gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt
     6601 agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga
     6661 cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac
     6721 tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
     6781 aaaggacgaa acaccgataa cggtgagcaa gggcggtttt agagctagaa atagcaagtt
     6841 aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttttgtt
     6901 ttagagctag aaatagcaag ttaaaataag gctagtccgt ttttagcgcg tgcgccaatt
     6961 ctgcagacaa atggcggatc ccgagagcat aatattgata tgtgccaaag ttgtttctga
     7021 ctgactaata agtataattt gtttctatta tgtataggtt aagctaatta cttattttat
     7081 aatacaacat gactgttttt aaagtacaaa ataagtttat ttttgtaaaa gagagaatgt
     7141 ttaaaagttt tgttacttta tagaagaaat tttgagtttt tgtttttttt taataaataa
     7201 ataaacataa ataaattgtt tgttgaattt attattagta tgtaagtgta aatataataa
     7261 aacttaatat ctattcaaat taataaataa acctcgatat acagaccgat aaaacacatg
     7321 cgtcaatttt acgcatgatt atctttaacg tacgtcacaa tatgattatc tttctagggt
     7381 taaataatag tttctaattt ttttattatt cagcctgctg tcgtgaatac cgagctccaa
     7441 ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac gtcgtgactg
     7501 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     7561 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     7621 cgaatggcgc gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     7681 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     7741 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     7801 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     7861 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     7921 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     7981 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     8041 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcc
//
