LOCUS       Exported                7254 bp DNA     circular SYN 09-SEP-2023
DEFINITION  pxl-T1 sgRNA-CMV-?¡Âpuro+T1-T2A-eGFP-polyA-ipuro rotated to position 
            122.
ACCESSION   .
VERSION     .
KEYWORDS    r-pxl-T1 sgRNA-CMV-?puro+T1-T2A-eGFP-polyA-ipuro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 9, 2023 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    7..246
                     /label=U6 pro
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    247..266
                     /label=T1
                     /note="color: #999999; direction: RIGHT"
     misc_feature    267..348
                     /label=sgRNA
                     /note="color: #00ccff; direction: RIGHT"
     misc_feature    357..1011
                     /label=CMV pro
                     /note="color: #ffffff; direction: RIGHT"
     CDS             922..1542
                     /codon_start=1
                     /label=ORF-3
                     /note="color: #cc0000"
                     /translation="MGGRRVRWEVYISRALWLTREPTAYWLIEIGSMTEYKPTVRLATR
                     DDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAA
                     VAVWSSPNVSRGRPPGAAPAQGARVVPGHRRRLARPPGQGSGQRRRAPRSGGGRARRGA
                     RLPGDLRAPQPPLLRAARLHRHRRRRGARRTAHLVHDPQARCR"
     CDS             1018..1246
                     /codon_start=1
                     /label=deta-puro
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVW"
     misc_feature    1247..1269
                     /label=T1+PAM
                     /note="color: #999999; direction: RIGHT"
     misc_feature    1270..1537
                     /label=deta-puro
                     /note="color: #cc0000; direction: RIGHT"
     CDS             1517..2338
                     /codon_start=1
                     /label=ORF-2
                     /note="color: #cc0000"
                     /translation="MTRKPGAASTMEVDGSGEGRGSLLTCGDVEENPGPVSKGEELFTG
                     VVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQ
                     CFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGI
                     DFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTP
                     IGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     misc_feature    1559..1621
                     /label=T2A
                     /note="color: #cc99b2; direction: RIGHT"
     CDS             1622..2338
                     /codon_start=1
                     /label=eGFP
                     /note="color: #00ff00"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     misc_feature    2345..2651
                     /label=polyA
                     /note="color: #999999; direction: RIGHT"
     misc_feature    2691..3284
                     /label=ipuro
                     /note="color: #ccffcc; direction: RIGHT"
     misc_feature    3321..3633
                     /label=5' TR
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    4124..4246
                     /label=ColE1 ori
                     /note="color: #ffff00; direction: RIGHT"
     misc_feature    4124..4246
                     /label=alpha LacZ gene
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    5283..6140
                     /label=Amp
                     /note="color: #ccffcc; direction: RIGHT"
     misc_feature    6272..6728
                     /label=F1 ori
                     /note="color: #ffff00; direction: RIGHT"
     misc_feature    7087..7245
                     /label=3' TR
                     /note="color: #cc0000; direction: RIGHT"
ORIGIN
        1 gaattctttc ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata
       61 attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag
      121 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg
      181 cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
      241 aacaccgttc tccgaacgtg tcacgtgttt tagagctaga aatagcaagt taaaataagg
      301 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcttttttgc ggccgccgat
      361 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
      421 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
      481 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      541 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      601 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
      661 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactctcct
      721 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      781 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      841 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
      901 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      961 agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat tggatccatg
     1021 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     1081 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     1141 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     1201 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggagttc tccgaacgtg
     1261 tcacgtggga ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     1321 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     1381 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     1441 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     1501 accgcgcacc tggtgcatga cccgcaagcc cggtgccgct agcaccatgg aggtcgacgg
     1561 cagtggagag ggcagaggaa gtctgctaac atgcggtgac gtcgaggaga atcctggccc
     1621 agtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     1681 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     1741 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     1801 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     1861 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     1921 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     1981 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     2041 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     2101 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     2161 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     2221 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     2281 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaact
     2341 cgagggatca attctctagg gctcgctgat cagcctcgac tgtgccttct agttgccagc
     2401 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     2461 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     2521 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     2581 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctcgatcc
     2641 tctagagtcg aagatcttaa tacgactcac tatagggcga attgggtacc accgagtaca
     2701 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     2761 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     2821 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     2881 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     2941 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     3001 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     3061 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     3121 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     3181 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     3241 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgccgggccc cccctcgagg
     3301 tcgacggtat cgataagctt gatatctata acaagaaaat atatatataa taagttatca
     3361 cgtaagtaga acatgaaata acaatataat tatcgtatga gttaaatctt aaaagtcacg
     3421 taaaagataa tcatgcgtca ttttgactca cgcggtcgtt atagttcaaa atcagtgaca
     3481 cttaccgcat tgacaagcac gcctcacggg agctccaagc ggcgactgag atgtcctaaa
     3541 tgcacagcga cggattcgcg ctatttagaa agagagagca atatttcaag aatgcatgcg
     3601 tcaattttac gcagactatc tttctagggt taatctagct gcatcaggat catatcgtcg
     3661 ggtctttttt ccggctcagt catcgcccaa gctggcgcta tctgggcatc ggggaggaag
     3721 aagcccgtgc cttttcccgc gaggttgaag cggcatggaa agagtttgcc gaggatgact
     3781 gctgctgcat tgacgttgag cgaaaacgca cgtttaccat gatgattcgg gaaggtgtgg
     3841 ccatgcacgc ctttaacggt gaactgttcg ttcaggccac ctgggatacc agttcgtcgc
     3901 ggcttttccg gacacagttc cggatggtca gcccgaagcg catcagcaac ccgaacaata
     3961 ccggcgacag ccggaactgc cgtgccggtg tgcagattaa tgacagcggt gcggcgctgg
     4021 gatattacgt cagcgaggac gggtatcctg gctggatgcc gcagaaatgg acatggatac
     4081 cccgtgagtt acccggcggg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     4141 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     4201 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     4261 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     4321 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     4381 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     4441 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     4501 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     4561 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     4621 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     4681 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     4741 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     4801 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     4861 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     4921 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     4981 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     5041 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     5101 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     5161 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     5221 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     5281 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     5341 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     5401 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     5461 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     5521 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     5581 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     5641 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     5701 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     5761 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     5821 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     5881 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     5941 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     6001 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     6061 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     6121 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     6181 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     6241 tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa
     6301 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     6361 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     6421 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     6481 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     6541 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     6601 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     6661 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     6721 ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     6781 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     6841 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcctcgttc
     6901 attcacgttt ttgaacccgt ggaggacggg cagactcgcg gtgcaaatgt gttttacagc
     6961 gtgatggagc agatgaagat gctcgacacg ctgcagaaca cgcagctaga ttaaccctag
     7021 aaagataatc atattgtgac gtacgttaaa gataatcatg cgtaaaattg acgcatgtgt
     7081 tttatcggtc tgtatatcga ggtttattta ttaatttgaa tagatattaa gttttattat
     7141 atttacactt acatactaat aataaattca acaaacaatt tatttatgtt tatttattta
     7201 ttaaaaaaaa acaaaaactc aaaatttctt ctataaagta acaaaacttt tatc
//
