LOCUS       Exported                7725 bp ds-DNA     circular SYN 13-SEP-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pXK-1649 pcDNA3.1-CMV-PuroR.L-mCherry-repeat.PuroR.R-T2A-eGFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 14, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7725
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(203..212)
                     /label=1027
     primer_bind     209..229
                     /label=965
     primer_bind     209..229
                     /label=955
     primer_bind     209..229
                     /label=2845
     primer_bind     232..253
                     /label=2069
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     248..268
                     /label=731
     primer_bind     251..274
                     /label=1384
     primer_bind     298..316
                     /label=2760
     primer_bind     complement(300..319)
                     /label=2747
     primer_bind     308..333
                     /label=2127
     primer_bind     complement(311..330)
                     /label=3150
     primer_bind     311..330
                     /label=3149
     primer_bind     312..334
                     /label=1711
     primer_bind     complement(331..350)
                     /label=3166
     primer_bind     complement(394..414)
                     /label=2743
     primer_bind     complement(429..450)
                     /label=2272
     primer_bind     complement(524..537)
                     /label=3166
     primer_bind     564..581
                     /label=1375
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     736..755
                     /label=pCMV5F
     primer_bind     769..789
                     /label=CMV-F
     primer_bind     780..797
                     /label=pFlag-CMV-F:
     primer_bind     781..800
                     /label=PIRES2-EGFP-P5
     primer_bind     795..816
                     /label=pEGFP-N5
     primer_bind     complement(800..819)
                     /label=2070
     primer_bind     complement(839..858)
                     /label=2944
     primer_bind     complement(841..864)
                     /label=2893
     primer_bind     863..883
                     /label=1893
     primer_bind     863..882
                     /label=T7
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     863..881
                     /label=2159
     primer_bind     863..880
                     /label=T72G
     primer_bind     911..934
                     /label=BS-3-Hind3-F
     primer_bind     917..940
                     /label=1517
     primer_bind     918..940
                     /label=2841
     misc_feature    920..1224
                     /label=PuroR.L
     primer_bind     920..937
                     /label=1080
     primer_bind     923..944
                     /label=2847
     primer_bind     923..943
                     /label=1513
     primer_bind     923..943
                     /label=1900
     primer_bind     923..941
                     /label=1850
     primer_bind     923..935
                     /label=1619
     primer_bind     1025..1044
                     /label=1526
     primer_bind     1040..1059
                     /label=1535
                     /note="2014.4.21"
     primer_bind     complement(1054..1074)
                     /label=1518
     primer_bind     complement(1067..1085)
                     /label=2681
     primer_bind     complement(1105..1124)
                     /label=1519
     primer_bind     1120..1131
                     /label=729
     primer_bind     1120..1129
                     /label=2577
     primer_bind     complement(1131..1148)
                     /label=2985
     primer_bind     complement(1131..1148)
                     /label=2983
     primer_bind     complement(1131..1148)
                     /label=2981
     primer_bind     complement(1131..1148)
                     /label=2842
     primer_bind     1132..1142
                     /label=2625
     primer_bind     1141..1163
                     /label=415
     primer_bind     complement(1154..1177)
                     /label=1380
     primer_bind     complement(1156..1175)
                     /label=1520
     primer_bind     complement(1156..1167)
                     /label=ACYCDuetUP1
     primer_bind     1171..1187
                     /label=1381
     primer_bind     1176..1193
                     /label=2949
     primer_bind     1192..1201
                     /label=1067
     primer_bind     1192..1201
                     /label=3105
     primer_bind     1192..1201
                     /label=1017
     primer_bind     complement(1205..1230)
                     /label=1521
     primer_bind     complement(1222..1231)
                     /label=2338
     primer_bind     1223..1273
                     /label=1019
     primer_bind     1225..1271
                     /label=hAAVS1-sgP-F
     primer_bind     complement(1232..1255)
                     /label=1653
     primer_bind     1232..1255
                     /label=1652
     primer_bind     1232..1250
                     /label=1779
     misc_feature    1233..1255
                     /label=AAVS1.T
     primer_bind     complement(1233..1253)
                     /label=2882
     primer_bind     1255..1274
                     /label=2077
     CDS             1256..1966
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1256..1274
                     /label=2238
     primer_bind     1256..1274
                     /label=1475
     primer_bind     1256..1274
                     /label=1907
     primer_bind     complement(1256..1273)
                     /label=1491
     primer_bind     1256..1273
                     /label=725
     primer_bind     1256..1269
                     /label=2637
     primer_bind     1256..1268
                     /label=BS-1-b
     primer_bind     1259..1277
                     /label=3032
     primer_bind     1328..1368
                     /label=727
     primer_bind     1343..1361
                     /label=1201
     primer_bind     1361..1374
                     /label=727
     primer_bind     1373..1414
                     /label=2635
     primer_bind     1411..1422
                     /label=2637
     primer_bind     complement(1556..1573)
                     /label=1376
     primer_bind     complement(1573..1632)
                     /label=2658
     primer_bind     complement(1578..1632)
                     /label=2657
     primer_bind     complement(1583..1641)
                     /label=1500
     primer_bind     1583..1641
                     /label=1499
     primer_bind     complement(1583..1632)
                     /label=2656
     primer_bind     complement(1588..1632)
                     /label=2655
     primer_bind     complement(1595..1613)
                     /label=1200
     primer_bind     1613..1632
                     /label=2659
     primer_bind     1613..1630
                     /label=1377
     primer_bind     complement(1617..1642)
                     /label=1029
     primer_bind     1702..1712
                     /label=1598
     primer_bind     1772..1789
                     /label=1497
     primer_bind     1786..1805
                     /label=2751
     primer_bind     1823..1832
                     /label=1064
     primer_bind     1880..1900
                     /label=2301
     primer_bind     complement(1926..1942)
                     /label=2651
     primer_bind     complement(1929..1942)
                     /label=2576
     primer_bind     complement(1931..1942)
                     /label=1378
     primer_bind     complement(1942..1964)
                     /label=2243
     primer_bind     complement(1943..1965)
                     /label=3034
     primer_bind     complement(1944..1963)
                     /label=1908
     primer_bind     complement(1945..1966)
                     /label=1476
     primer_bind     complement(1947..1966)
                     /label=2638
     primer_bind     complement(1948..1965)
                     /label=2761
     primer_bind     complement(1948..1965)
                     /label=2078
     primer_bind     complement(1949..1966)
                     /label=728
     primer_bind     complement(1951..1997)
                     /label=hAAVS1-sgP-R
     primer_bind     complement(1951..1997)
                     /label=BS-2
     primer_bind     complement(1952..1966)
                     /label=BS-4-Xbal1-R
     misc_feature    1967..1989
                     /label=AAVS1.T
     primer_bind     1967..1989
                     /label=1652
     primer_bind     complement(1967..1987)
                     /label=2882
     primer_bind     1967..1984
                     /label=1779
     primer_bind     1990..2020
                     /label=1526
     misc_feature    1998..2000
                     /label=stop codon
     misc_feature    2001..2200
                     /label=repeat
     primer_bind     2016..2035
                     /label=1535
                     /note="2014.4.21"
     primer_bind     complement(2030..2050)
                     /label=1518
     primer_bind     complement(2043..2061)
                     /label=2681
     primer_bind     complement(2081..2100)
                     /label=1519
     primer_bind     2096..2107
                     /label=729
     primer_bind     2096..2105
                     /label=2577
     primer_bind     complement(2107..2124)
                     /label=2985
     primer_bind     complement(2107..2124)
                     /label=2983
     primer_bind     complement(2107..2124)
                     /label=2981
     primer_bind     complement(2107..2124)
                     /label=2842
     primer_bind     2108..2118
                     /label=2625
     primer_bind     2117..2139
                     /label=415
     primer_bind     complement(2130..2153)
                     /label=1380
     primer_bind     complement(2132..2151)
                     /label=1520
     primer_bind     complement(2132..2143)
                     /label=ACYCDuetUP1
     primer_bind     2147..2163
                     /label=1381
     primer_bind     2152..2169
                     /label=2949
     primer_bind     2168..2177
                     /label=1067
     primer_bind     2168..2177
                     /label=3105
     primer_bind     2168..2177
                     /label=1017
     primer_bind     complement(2181..2201)
                     /label=1521
     misc_feature    2201..2492
                     /label=PuroR
     primer_bind     complement(2201..2211)
                     /label=2172
     primer_bind     2221..2243
                     /label=2982
     primer_bind     2221..2243
                     /label=2984
     primer_bind     2222..2243
                     /label=2986
     primer_bind     2225..2243
                     /label=2843
     primer_bind     2225..2241
                     /label=2980
     primer_bind     2225..2240
                     /label=2964
     primer_bind     complement(2232..2250)
                     /label=1522
     primer_bind     complement(2248..2259)
                     /label=2176
     primer_bind     2253..2308
                     /label=727
     primer_bind     complement(2282..2302)
                     /label=1523
     primer_bind     complement(2317..2329)
                     /label=729
     primer_bind     complement(2332..2351)
                     /label=1524
     primer_bind     complement(2356..2392)
                     /label=407
     primer_bind     complement(2356..2365)
                     /label=481
     primer_bind     complement(2356..2365)
                     /label=479
     primer_bind     complement(2356..2365)
                     /label=475
     primer_bind     complement(2356..2365)
                     /label=509
     primer_bind     complement(2356..2365)
                     /label=480
     primer_bind     complement(2356..2365)
                     /label=478
     primer_bind     complement(2356..2365)
                     /label=476
     primer_bind     complement(2356..2365)
                     /label=474
     primer_bind     complement(2356..2365)
                     /label=477
     primer_bind     complement(2356..2365)
                     /label=473
     primer_bind     complement(2380..2390)
                     /label=3032
     primer_bind     complement(2382..2400)
                     /label=1525
     primer_bind     2392..2412
                     /label=Puro-F
     primer_bind     complement(2474..2510)
                     /label=1514
     primer_bind     complement(2474..2492)
                     /label=1901
     primer_bind     complement(2475..2495)
                     /label=2844
     primer_bind     complement(2475..2493)
                     /label=2848
     primer_bind     complement(2478..2492)
                     /label=1081
     primer_bind     complement(2480..2492)
                     /label=1383
     primer_bind     2491..2553
                     /label=1902
     primer_bind     2491..2553
                     /label=2258
     primer_bind     2491..2521
                     /label=1844
     primer_bind     2491..2519
                     /label=1843
     primer_bind     2502..2526
                     /label=1515
     misc_feature    2508..2570
                     /label=T2A
     primer_bind     2508..2527
                     /label=245
     primer_bind     complement(2514..2538)
                     /label=1908
     primer_bind     2514..2527
                     /label=520
     primer_bind     2514..2527
                     /label=395
     primer_bind     2514..2525
                     /label=715
     primer_bind     2514..2525
                     /label=611
     primer_bind     complement(2519..2570)
                     /label=1909
     primer_bind     complement(2527..2571)
                     /label=2608
     primer_bind     complement(2527..2548)
                     /label=2677
     primer_bind     complement(2528..2569)
                     /label=193
     primer_bind     2533..2570
                     /label=1900
     primer_bind     complement(2548..2569)
                     /label=236
     primer_bind     complement(2551..2574)
                     /label=521
     primer_bind     complement(2551..2573)
                     /label=396
     primer_bind     complement(2552..2571)
                     /label=716
     primer_bind     complement(2552..2570)
                     /label=612
     primer_bind     2565..2585
                     /label=725
     CDS             2571..3287
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     2571..2591
                     /label=1936
     primer_bind     2571..2590
                     /label=1941
     primer_bind     2571..2589
                     /label=1891
     primer_bind     2571..2586
                     /label=2238
     primer_bind     2571..2586
                     /label=2077
     primer_bind     2571..2586
                     /label=1475
     primer_bind     2571..2586
                     /label=1907
     primer_bind     complement(2571..2585)
                     /label=1491
     primer_bind     2571..2585
                     /label=1019
     primer_bind     2571..2583
                     /label=hAAVS1-sgP-F
     primer_bind     2571..2581
                     /label=2637
     primer_bind     complement(2609..2625)
                     /label=1622
     primer_bind     complement(2613..2634)
                     /label=pEGFP-N3(EGFP-N)
     primer_bind     2630..2641
                     /label=631
     primer_bind     2644..2661
                     /label=1386
     primer_bind     2645..2663
                     /label=1918
     primer_bind     2645..2663
                     /label=1889
     primer_bind     2646..2664
                     /label=1925
     primer_bind     2646..2664
                     /label=1923
     primer_bind     2657..2674
                     /label=727
     primer_bind     2711..2768
                     /label=1926
     primer_bind     complement(2718..2745)
                     /label=2848
     primer_bind     2732..2741
                     /label=1472
     primer_bind     2749..2768
                     /label=1928
     primer_bind     2749..2768
                     /label=1927
     primer_bind     2796..2814
                     /label=1878
     primer_bind     2796..2814
                     /label=1893
     primer_bind     2800..2819
                     /label=1930
     primer_bind     2800..2819
                     /label=1933
     primer_bind     2800..2819
                     /label=1932
     primer_bind     complement(2820..2879)
                     /label=2665
     primer_bind     complement(2822..2842)
                     /label=2239
     primer_bind     complement(2825..2879)
                     /label=2664
     primer_bind     2829..2859
                     /label=1934
     primer_bind     complement(2830..2888)
                     /label=1498
     primer_bind     2830..2888
                     /label=1497
     primer_bind     complement(2830..2879)
                     /label=2663
     primer_bind     complement(2835..2879)
                     /label=2662
     primer_bind     complement(2835..2879)
                     /label=2673
     primer_bind     complement(2836..2879)
                     /label=2672
     primer_bind     complement(2837..2879)
                     /label=2671
     primer_bind     complement(2838..2879)
                     /label=2670
     primer_bind     complement(2839..2857)
                     /label=726
     primer_bind     complement(2840..2889)
                     /label=1935
     primer_bind     2840..2859
                     /label=2240
     primer_bind     complement(2841..2858)
                     /label=868
     primer_bind     complement(2841..2858)
                     /label=382
     primer_bind     2842..2899
                     /label=867
     primer_bind     2842..2899
                     /label=381
     primer_bind     complement(2843..2859)
                     /label=1385
     primer_bind     complement(2856..2876)
                     /label=2946
     primer_bind     complement(2858..2882)
                     /label=730
     primer_bind     2859..2877
                     /label=1374
     primer_bind     2860..2879
                     /label=2666
     primer_bind     2860..2877
                     /label=1492
     primer_bind     2864..2882
                     /label=2577
     primer_bind     2866..2886
                     /label=2678
     primer_bind     complement(2869..2885)
                     /label=1940
     primer_bind     complement(2900..2918)
                     /label=1931
     primer_bind     complement(2903..2921)
                     /label=1879
     primer_bind     2925..2999
                     /label=204
     primer_bind     complement(2935..2953)
                     /label=2578
     primer_bind     complement(2950..2969)
                     /label=1929
     primer_bind     2957..2972
                     /label=2577
     primer_bind     complement(2959..2980)
                     /label=1940
     primer_bind     2978..2998
                     /label=1938
     primer_bind     3038..3059
                     /label=2947
     primer_bind     complement(3040..3059)
                     /label=2683
     primer_bind     complement(3056..3075)
                     /label=1890
     primer_bind     complement(3056..3074)
                     /label=1924
     primer_bind     complement(3056..3074)
                     /label=1919
     primer_bind     3063..3081
                     /label=2273
     primer_bind     complement(3079..3108)
                     /label=1656
     primer_bind     complement(3080..3098)
                     /label=2241
     primer_bind     complement(3089..3099)
                     /label=850
     primer_bind     3098..3115
                     /label=2242
     primer_bind     3108..3127
                     /label=2300
     primer_bind     3157..3175
                     /label=2265
     primer_bind     3157..3175
                     /label=1781
     primer_bind     3190..3209
                     /label=2740
     primer_bind     3221..3242
                     /label=pEGFP-C-5':
     primer_bind     complement(3263..3285)
                     /label=2243
     primer_bind     complement(3264..3286)
                     /label=3034
     primer_bind     complement(3265..3284)
                     /label=1908
     primer_bind     complement(3266..3294)
                     /label=1476
     primer_bind     complement(3268..3307)
                     /label=2638
     primer_bind     complement(3269..3294)
                     /label=2078
     primer_bind     complement(3269..3286)
                     /label=2761
     primer_bind     complement(3270..3294)
                     /label=728
     primer_bind     complement(3272..3287)
                     /label=hAAVS1-sgP-R
     primer_bind     complement(3272..3287)
                     /label=BS-2
     primer_bind     complement(3273..3293)
                     /label=BS-4-Xbal1-R
     primer_bind     3319..3340
                     /label=2140
     primer_bind     complement(3319..3336)
                     /label=BGH
     primer_bind     3322..3341
                     /label=1961
     primer_bind     complement(3323..3341)
                     /label=1868
     polyA_signal    3325..3549
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3330..3354
                     /label=1976
     primer_bind     3337..3355
                     /label=2269
     primer_bind     3340..3359
                     /label=3163
     primer_bind     complement(3361..3382)
                     /label=1564
     primer_bind     complement(3388..3400)
                     /label=pFlag-CMV-R:
     primer_bind     complement(3432..3453)
                     /label=2679
     primer_bind     3432..3451
                     /label=2749
     primer_bind     3432..3451
                     /label=2267
     primer_bind     3432..3451
                     /label=1783
     primer_bind     3449..3468
                     /label=2757
     primer_bind     complement(3466..3475)
                     /label=2167
     primer_bind     complement(3467..3483)
                     /label=389
     primer_bind     complement(3506..3525)
                     /label=3164
     primer_bind     complement(3514..3533)
                     /label=966
     primer_bind     complement(3517..3532)
                     /label=3037
     primer_bind     complement(3523..3540)
                     /label=2948
     primer_bind     complement(3531..3553)
                     /label=1599
     primer_bind     complement(3531..3552)
                     /label=2260
     primer_bind     complement(3537..3553)
                     /label=2660
     rep_origin      3595..4023
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     3675..3698
                     /label=3113
     primer_bind     4011..4030
                     /label=1905
     primer_bind     complement(4032..4052)
                     /label=PBABE-R
     primer_bind     4034..4053
                     /label=527
     primer_bind     complement(4053..4081)
                     /label=856
     primer_bind     complement(4053..4081)
                     /label=376
     primer_bind     4076..4091
                     /label=387
     primer_bind     4076..4089
                     /label=385
     primer_bind     complement(4100..4119)
                     /label=pLXSN-R
     primer_bind     complement(4125..4153)
                     /label=856
     primer_bind     complement(4125..4153)
                     /label=376
     primer_bind     4148..4163
                     /label=387
     primer_bind     4148..4161
                     /label=385
     primer_bind     complement(4172..4191)
                     /label=pLXSN-R
     rep_origin      4217..4352
                     /label=SV40 Pro
                     /note="SV40 origin of replication"
     primer_bind     4428..4453
                     /label=2254
     primer_bind     4428..4448
                     /label=1477
     CDS             4433..5227
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     4529..4538
                     /label=209
     primer_bind     4670..4688
                     /label=2256
     primer_bind     complement(4792..4802)
                     /label=1032
     primer_bind     complement(4852..4870)
                     /label=2255
     primer_bind     complement(4880..4889)
                     /label=NOS-R
     primer_bind     complement(5204..5224)
                     /label=2257
     primer_bind     complement(5208..5227)
                     /label=388
     primer_bind     5208..5227
                     /label=389
     primer_bind     complement(5246..5265)
                     /label=528
     primer_bind     5299..5349
                     /label=2577
     primer_bind     complement(5380..5399)
                     /label=1478
     polyA_signal    5401..5522
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     5401..5420
                     /label=2573
     primer_bind     complement(5438..5457)
                     /label=SV40-PArev
     primer_bind     5447..5468
                     /label=3168
     primer_bind     complement(5473..5492)
                     /label=3156
     primer_bind     5473..5492
                     /label=3155
     primer_bind     5477..5496
                     /label=pIRES.R:
     primer_bind     5492..5511
                     /label=EBV-R
     primer_bind     complement(5499..5522)
                     /label=1516
     primer_bind     5500..5519
                     /label=pCMV-R
     primer_bind     complement(5501..5522)
                     /label=1869
     primer_bind     complement(5504..5523)
                     /label=3158
     primer_bind     5504..5523
                     /label=3157
     primer_bind     complement(5505..5522)
                     /label=734
     primer_bind     complement(5512..5531)
                     /label=2574
     primer_bind     complement(5514..5531)
                     /label=2649
     primer_bind     complement(5515..5534)
                     /label=1906
     primer_bind     complement(5570..5587)
                     /label=M13R
     primer_bind     complement(5571..5590)
                     /label=1626
     primer_bind     complement(5571..5588)
                     /label=750
     primer_bind     complement(5583..5606)
                     /label=M13R-48
     primer_bind     complement(5589..5608)
                     /label=pQE30-F (2)
     primer_bind     complement(5610..5632)
                     /label=221
     primer_bind     complement(5633..5652)
                     /label=pTarget.R
     primer_bind     complement(5636..5655)
                     /label=PCAMBIA1301-F
     primer_bind     complement(5659..5679)
                     /label=3.0rev:
     primer_bind     complement(5710..5773)
                     /label=2621
     primer_bind     5751..5760
                     /label=1067
     primer_bind     5751..5760
                     /label=1017
     primer_bind     complement(5773..5806)
                     /label=488'
     primer_bind     complement(5773..5806)
                     /label=488
     primer_bind     5864..5882
                     /label=957
     primer_bind     5864..5882
                     /label=947
     primer_bind     5917..5937
                     /label=2072
     primer_bind     complement(5921..5940)
                     /label=1620
     primer_bind     complement(5932..5959)
                     /label=1620
     primer_bind     complement(5946..5965)
                     /label=U6-F-Plus?
     primer_bind     5957..5977
                     /label=367
     primer_bind     5957..5976
                     /label=3186
     rep_origin      complement(5973..6558)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6103..6115
                     /label=815
     CDS             complement(6729..7589)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6845..6858)
                     /label=2917
     primer_bind     6876..6886
                     /label=1380
     primer_bind     complement(7514..7524)
                     /label=739
     promoter        complement(7590..7694)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7640..7660)
                     /label=366
     primer_bind     complement(7641..7661)
                     /label=1588
     primer_bind     complement(7675..7694)
                     /label=2071
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
      961 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     1021 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     1081 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     1141 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     1201 catggccgag ttgagcggtt cccgggatcc tgctgtcccc tccaccccac agtggatggt
     1261 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
     1321 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     1381 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     1441 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
     1501 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     1561 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     1621 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     1681 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     1741 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     1801 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     1861 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     1921 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaactgt cccctccacc
     1981 ccacagtggg cggccgctaa accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     2041 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     2101 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     2161 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     2221 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     2281 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     2341 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     2401 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     2461 gcacctggtg catgacccgc aagcccggtg ccgctcgagc taccggtggc agtggagagg
     2521 gcagaggaag tctgctaaca tgcggtgacg tcgaggagaa tcctggccca gtgagcaagg
     2581 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     2641 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     2701 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     2761 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     2821 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     2881 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     2941 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     3001 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     3061 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     3121 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     3181 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     3241 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaatct agagggcccg
     3301 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     3361 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     3421 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     3481 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     3541 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     3601 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     3661 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     3721 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     3781 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     3841 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     3901 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     3961 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     4021 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     4081 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     4141 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     4201 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     4261 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     4321 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     4381 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
     4441 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     4501 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     4561 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     4621 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     4681 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     4741 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     4801 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     4861 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     4921 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     4981 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     5041 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     5101 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     5161 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     5221 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     5281 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     5341 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     5401 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     5461 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     5521 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     5581 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     5641 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     5701 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     5761 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     5821 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     5881 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     5941 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     6001 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     6061 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     6121 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     6181 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     6241 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     6301 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     6361 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     6421 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     6481 tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca gattacgcgc
     6541 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     6601 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     6661 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     6721 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     6781 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     6841 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     6901 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     6961 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     7021 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     7081 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     7141 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     7201 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     7261 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     7321 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     7381 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     7441 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     7501 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     7561 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     7621 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     7681 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
