LOCUS       Exported                7807 bp ds-DNA     circular SYN 13-SEP-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pXK-1650 pcDNA3.1-iPuroR-CMV-PuroR.L-mCherry-PuroR.R-T2A-eGFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 14, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7807
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     12..34
                     /label=BS-9
     misc_feature    18..611
                     /label=puro
     CDS             18..611
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=iPuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     18..29
                     /label=BS-3
     primer_bind     complement(224..237)
                     /label=BS-7
     primer_bind     complement(251..262)
                     /label=ACYCDuetUP1
     primer_bind     343..356
                     /label=BS-8
     primer_bind     complement(473..482)
                     /label=BS-7
     primer_bind     511..531
                     /label=Puro-F
     primer_bind     complement(599..623)
                     /label=BS-10
     enhancer        625..1004
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1005..1208
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1126..1145
                     /label=pCMV5F
     primer_bind     1159..1179
                     /label=CMV-F
     primer_bind     1159..1179
                     /label=CMV -F
     primer_bind     1170..1187
                     /label=pFlag-CMV-F:
     primer_bind     1171..1190
                     /label=pIRES2-EGFP.P5':
     primer_bind     1171..1190
                     /label=PIRES2-EGFP-P5
     primer_bind     1185..1206
                     /label=pEGFP-N5
     primer_bind     1185..1206
                     /label=pEGFP-N-5':
     primer_bind     1253..1272
                     /label=T7 (2)
     primer_bind     1253..1272
                     /label=T7
     promoter        1253..1271
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1253..1270
                     /label=T72G
     primer_bind     1301..1324
                     /label=BS-3
     misc_feature    1310..1531
                     /label=PuroR-L
     primer_bind     1313..1329
                     /label=BS-9
     primer_bind     complement(1519..1532)
                     /label=BS-7
     primer_bind     1529..1577
                     /label=hAAVS1-sgP-F
     misc_feature    1539..1561
                     /label=Target
     CDS             1562..2272
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2257..2303)
                     /label=hAAVS1-sgP-R
     primer_bind     complement(2257..2303)
                     /label=BS-2
     primer_bind     complement(2258..2272)
                     /label=BS-4-Xbal1-R
     misc_feature    2273..2295
                     /label=Target
     primer_bind     2296..2319
                     /label=BS-8
     misc_feature    2307..2574
                     /label=PuroR-R
     primer_bind     complement(2436..2445)
                     /label=BS-7
     primer_bind     2474..2494
                     /label=Puro-F
     primer_bind     complement(2562..2574)
                     /label=BS-10
     CDS             2599..2652
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2653..3369
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     2653..2665
                     /label=hAAVS1-sgP-F
     primer_bind     complement(2695..2716)
                     /label=pEGFP-N3(EGFP-N)
     primer_bind     complement(2695..2716)
                     /label=pEGFP-N-3':
     primer_bind     3303..3324
                     /label=pEGFP-C5(EGFP-C)
     primer_bind     3303..3324
                     /label=pEGFP-C-5':
     primer_bind     complement(3354..3369)
                     /label=hAAVS1-sgP-R
     primer_bind     complement(3354..3369)
                     /label=BS-2
     primer_bind     complement(3355..3375)
                     /label=BS-4-Xbal1-R
     primer_bind     complement(3401..3418)
                     /label=pcDNA3.1R
     primer_bind     complement(3401..3418)
                     /label=BGH
     polyA_signal    3407..3631
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3470..3482)
                     /label=pFlag-CMV-R:
     rep_origin      3677..4105
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4114..4134)
                     /label=PBABE-R
     primer_bind     complement(4182..4201)
                     /label=pLXSN-R (2)
     primer_bind     complement(4182..4201)
                     /label=pLXSN-R
     primer_bind     complement(4254..4273)
                     /label=pLXSN-R (2)
     primer_bind     complement(4254..4273)
                     /label=pLXSN-R
     rep_origin      4299..4434
                     /label=SV40 Pro
                     /note="SV40 origin of replication"
     CDS             4515..5309
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4962..4971)
                     /label=NOS-R
     polyA_signal    5483..5604
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5520..5539)
                     /label=SV40-PArev
     primer_bind     5559..5578
                     /label=pIRES.R:
     primer_bind     5574..5593
                     /label=EBV-R :
     primer_bind     5574..5593
                     /label=EBV-R
     primer_bind     5582..5601
                     /label=pCMV-R (2)
     primer_bind     5582..5601
                     /label=pCMV-R
     primer_bind     complement(5652..5669)
                     /label=M13R
     primer_bind     complement(5653..5669)
                     /label=M13R(-26)
     primer_bind     complement(5665..5688)
                     /label=M13R-48
     primer_bind     complement(5665..5688)
                     /label=M13R(-48)
     primer_bind     complement(5671..5690)
                     /label=pQE30-F (2)
     primer_bind     complement(5671..5690)
                     /label=pQE30-F
     primer_bind     complement(5715..5734)
                     /label=pTarget.R
     primer_bind     complement(5718..5737)
                     /label=PCAMBIA1301-F
     primer_bind     complement(5718..5737)
                     /label=P1
     primer_bind     complement(5741..5761)
                     /label=3.0rev:
     primer_bind     complement(6028..6047)
                     /label=U6-F-Plus?
     rep_origin      complement(6055..6640)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6811..7671)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7672..7776)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
       61 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
      121 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
      181 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
      241 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
      301 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
      361 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
      421 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
      481 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
      541 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
      601 agcccggtgc ctaatgaacg cgttgacatt gattattgac tagttattaa tagtaatcaa
      661 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      721 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      781 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      841 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      901 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      961 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     1021 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     1081 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     1141 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     1201 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
     1261 ctcactatag ggagacccaa gctggctagc gtttaaactt aagcttacca tgaccgagta
     1321 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     1381 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     1441 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     1501 gtgggtcgcg gacgacggcg ccgcggtggc ggatcctgct gtcccctcca ccccacagtg
     1561 gatggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
     1621 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     1681 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
     1741 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
     1801 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
     1861 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     1921 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     1981 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
     2041 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
     2101 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
     2161 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     2221 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt aactgtcccc
     2281 tccaccccac agtgggcggc cgctaaaggc ctcctggcgc cgcaccggcc caaggagccc
     2341 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     2401 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     2461 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     2521 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgccgctcga
     2581 gctaccggtg gcagtggaga gggcagagga agtctgctaa catgcggtga cgtcgaggag
     2641 aatcctggcc cagtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     2701 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     2761 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     2821 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     2881 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     2941 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     3001 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     3061 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     3121 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     3181 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     3241 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     3301 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     3361 tacaagtaat ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     3421 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     3481 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     3541 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     3601 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     3661 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     3721 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     3781 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     3841 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     3901 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     3961 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     4021 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     4081 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     4141 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4201 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     4261 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     4321 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     4381 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     4441 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat
     4501 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     4561 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     4621 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     4681 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     4741 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     4801 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     4861 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     4921 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     4981 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     5041 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     5101 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     5161 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     5221 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     5281 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     5341 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     5401 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     5461 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     5521 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     5581 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     5641 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     5701 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     5761 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     5821 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     5881 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     5941 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     6001 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     6061 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     6121 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     6181 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     6241 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     6301 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     6361 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     6421 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     6481 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     6541 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtttttttgt
     6601 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     6661 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     6721 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     6781 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     6841 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     6901 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     6961 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     7021 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     7081 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     7141 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     7201 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     7261 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     7321 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     7381 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     7441 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     7501 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     7561 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     7621 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     7681 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     7741 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     7801 tgacgtc
//
