LOCUS       Exported                9048 bp ds-DNA     circular SYN 06-SEP-2023
DEFINITION  stably express GFP-tagged NDP52 for CID induced mitophagy.
ACCESSION   .
VERSION     .
KEYWORDS    pHAGE-FKBP-GFP-NDP52
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9048)
  AUTHORS   Vargas JNS, Wang C, Bunker E, Hao L, Maric D, Schiavo G, Randow F, 
            Youle RJ
  TITLE     Spatiotemporal Control of ULK1 Activation by NDP52 and TBK1 during 
            Selective Autophagy.
  JOURNAL   Mol Cell. 2019 Apr 18;74(2):347-362.e6. doi: 
            10.1016/j.molcel.2019.02.010. Epub 2019 Mar 7.
  PUBMED    30853401
REFERENCE   2  (bases 1 to 9048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Sep 11, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9048
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     1948..1974
                     /label=SMP3608
     misc_feature    2062..2179
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2180..2201
                     /label=SMP2956
     primer_bind     2191..2210
                     /label=SMP2937
     primer_bind     2194..2214
                     /label=SMP2954
     enhancer        2231..2534
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(2376..2397)
                     /label=SMP1803
     promoter        2535..2738
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2564..2579)
                     /label=SMP2619
     primer_bind     2643..2664
                     /label=SMP2400
     primer_bind     2646..2665
                     /label=EGFP-N-for
     primer_bind     2683..2701
                     /label=SMP443
     primer_bind     2689..2709
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2702..2718
                     /label=IRES-for
     primer_bind     2717..2737
                     /label=SMP736
     primer_bind     2728..2747
                     /label=pTRE-for
     primer_bind     2735..2759
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     2735..2755
                     /label=SMP2952
     primer_bind     2735..2752
                     /label=CEP4-for
     primer_bind     2789..2805
                     /label=TET-CMV-for
     regulatory      2821..2830
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2827..3150
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     primer_bind     complement(3083..3094)
                     /label=SMP296
     primer_bind     3107..3126
                     /label=SMP3313
     CDS             3157..3477
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             3496..4209
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     3496..3513
                     /label=SMP2824
     primer_bind     complement(3496..3513)
                     /label=SMP3729
     primer_bind     3499..3516
                     /label=SMP2016
     primer_bind     complement(3535..3551)
                     /label=PEN1-737R
     primer_bind     complement(3538..3559)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3540..3560)
                     /label=SMP919
     primer_bind     complement(3555..3573)
                     /label=SMP2564
     primer_bind     complement(3566..3586)
                     /label=SMP2562
     primer_bind     complement(3568..3586)
                     /label=EGFP-N-R+33
     primer_bind     complement(3601..3619)
                     /label=EGFP-N-rev
     primer_bind     complement(3609..3628)
                     /label=SMP2433
     primer_bind     complement(3624..3642)
                     /label=SMP3388
     primer_bind     3667..3687
                     /label=SMP2316
     primer_bind     3741..3760
                     /label=SMP1001
     primer_bind     3787..3806
                     /label=SMP1325
     primer_bind     complement(3797..3817)
                     /label=SMP2969
     primer_bind     complement(3799..3818)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(3854..3874)
                     /label=SMP2971
     primer_bind     complement(3857..3873)
                     /label=SMP2578
     primer_bind     complement(3887..3900)
                     /label=SMP2969
     primer_bind     3900..3918
                     /label=SMP444
     primer_bind     3910..3934
                     /label=SMP1666
     primer_bind     3964..3981
                     /label=SMP3040
     primer_bind     3964..3981
                     /label=SMP3104
     primer_bind     3969..3988
                     /label=SMP1330
     primer_bind     3995..4012
                     /label=SMP4080
     primer_bind     3996..4015
                     /label=SMP437
     primer_bind     complement(3996..4015)
                     /label=SMP471
     primer_bind     4034..4051
                     /label=SMP3892
     primer_bind     4047..4066
                     /label=SMP364
     primer_bind     4096..4128
                     /label=SMP295
     primer_bind     complement(4096..4128)
                     /label=SMP296
     primer_bind     4119..4137
                     /label=EGFP-C-F-31
     primer_bind     4128..4144
                     /label=EGFP-for
     primer_bind     4134..4152
                     /label=SMP564
     primer_bind     4146..4167
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4150..4167
                     /label=EGFP-C-for
     primer_bind     4182..4219
                     /label=SMP2241
     primer_bind     complement(4182..4200)
                     /label=SMP2415
     primer_bind     complement(4189..4209)
                     /label=SMP2429
     primer_bind     4189..4209
                     /label=SMP3728
     primer_bind     complement(4189..4208)
                     /label=SMP2596
     primer_bind     complement(4191..4219)
                     /label=SMP4373
     primer_bind     complement(4207..4233)
                     /label=SMP884
     primer_bind     complement(4213..4233)
                     /label=SMP1201
     primer_bind     4216..4279
                     /label=Fragment.FOR
     primer_bind     complement(4231..4254)
                     /label=SMP1053
     misc_feature    4233..4254
                     /label=primer fw
     CDS             4255..5427
                     /codon_start=3
                     /label=NAP1 (no ATG)
                     /translation="DALVEDDICSSNHEKAHKRDTVTPVSIYSGDESVASHFALVTAYE
                     DIKKRLKDSEKENSLLKKRIRFLEEKLIARFEEETSSVGREQVNKAYHAYREVCIDRDN
                     LKSKLDKMNKDNSESLKVLNEQLQSKEVELLQLRTEVETQQVMRNLNPPSSNWEVEKLS
                     CDLKIHGLEQELELMRKECSDLKIELQKAKQTDPYQEDNLKSRDLQKLSISSDNMQHAY
                     WELKREMSNLHLVTQVQAELLRKLKTSTAIKKACAPVGCSEDLGRDSTKLHLMNFTATY
                     TRHPPLLPNGKALCHTTSSPLPGDVKVLSEKAILQSWTDNERSIPNDGTCFQEHSSYGR
                     NSLEDNSWVFPSPPKSSETAFGETKTKTLPLPNLPPLHYLDQHNQNCLYKN"
     misc_difference 4282..4284
                     /label=I11S
     misc_difference 4285..4287
                     /label=L12S
     primer_bind     complement(5393..5436)
                     /label=Fragment.REV
     misc_feature    5428..5452
                     /label=primer rev
     misc_feature    5431..5436
                     /label=BamHI
     misc_feature    5438..6026
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5491..5511)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     complement(5493..5516)
                     /label=SMP3898
     primer_bind     complement(5494..5514)
                     /label=SMP1896
     primer_bind     complement(5505..5524)
                     /label=WHV-5R
     CDS             complement(5909..5920)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6101..6334
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6446..6464)
                     /label=QE-for
     primer_bind     complement(6453..6471)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     6538..6558
                     /label=SMP646
     primer_bind     complement(6538..6558)
                     /label=SMP654
     promoter        6539..6643
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6644..7504
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6689..6708)
                     /label=AmpStart
     primer_bind     complement(6862..6881)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     7435..7454
                     /label=AmpStop
     rep_origin      7675..8263
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8055..8072
                     /label=SMP4077
     primer_bind     8164..8183
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8417..8434
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     8526..8545
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(8549..8560)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8642..8659
                     /label=SMP2212
     primer_bind     complement(8644..8661)
                     /label=SMP4078
     protein_bind    8674..8695
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8710..8740
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     8721..8740
                     /label=pUCM13-rev-157
     protein_bind    8748..8764
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8753..8775
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8755..8775
                     /label=SMP547
     primer_bind     8755..8774
                     /label=LacOp-for
     primer_bind     8767..8786
                     /label=SMP993
     primer_bind     8772..8788
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8772..8788
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8825..8846)
                     /label=SMP3893
     primer_bind     8838..8857
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttaattcaca
     2041 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     2101 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     2161 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggactag
     2221 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2281 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2341 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2401 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2461 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2521 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2581 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2641 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     2701 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     2761 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccggcgg ccgcggtacc
     2821 gccaccatgg gagtgcaggt ggaaaccatc tccccaggag acgggcgcac cttccccaag
     2881 cgcggccaga cctgcgtggt gcactacacc gggatgcttg aagatggaaa gaaatttgat
     2941 tcctcccggg acagaaacaa gccctttaag tttatgctag gcaagcagga ggtgatccga
     3001 ggctgggaag aaggggttgc ccagatgagt gtgggtcaga gagccaaact gactatatct
     3061 ccagattatg cctatggtgc cactgggcac ccaggcatca tcccaccaca tgccactctc
     3121 gtcttcgatg tggagcttct aaaactggaa actagaggcg tgcaggtcga gaccatcagc
     3181 cccggcgacg gccgcacctt tcccaagaga ggccagactt gcgtggtcca ctacaccggc
     3241 atgctggagg acggcaagaa gttcgacagc agccgcgacc gcaacaagcc cttcaagttc
     3301 atgctgggca aacaggaagt gatccgcggc tgggaggaag gcgtggctca gatgagcgtg
     3361 gggcagcggg ccaagctgac catcagcccc gactatgcct acggcgccac cggccacccc
     3421 ggcatcatcc ccccccacgc caccctggtg ttcgacgtgg agctgctgaa gctggagact
     3481 agtaccggtt ctagagtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     3541 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     3601 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     3661 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     3721 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     3781 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     3841 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     3901 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     3961 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     4021 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     4081 cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc
     4141 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     4201 ctgtacaagt ccggactcag atctcgagct caagcttcga attctgcagt cgacgatgca
     4261 ctggtagaag atgatatctg tagctccaat catgaaaaag cccataagag agatacagtg
     4321 actccagttt caatatattc aggagatgaa tctgttgctt cccattttgc tcttgtcact
     4381 gcatatgaag acatcaaaaa acgacttaag gattcagaga aagagaactc tttgttaaag
     4441 aagagaataa gatttttgga agaaaagcta atagctcgat ttgaagaaga aacaagttcc
     4501 gtgggacgag aacaagtaaa taaggcctat catgcatatc gagaggtttg cattgataga
     4561 gataatttga agagcaaact ggacaaaatg aataaagaca actctgaatc tttgaaagta
     4621 ttgaatgagc agctacaatc taaagaagta gaactcctcc agctgaggac agaggtggaa
     4681 actcagcagg tgatgaggaa tttaaatcca ccttcatcaa actgggaggt ggaaaagttg
     4741 agctgtgacc tgaagatcca tggtttggaa caagagctgg aactgatgag gaaagaatgt
     4801 agcgatctca aaatagaact acagaaagcc aaacaaacgg atccatatca ggaagacaat
     4861 ctgaagagca gagatctcca aaaactaagc atttcaagtg ataatatgca gcatgcatac
     4921 tgggaactga agagagaaat gtctaattta catctggtga ctcaagtaca agctgaacta
     4981 ctaagaaaac tgaaaacctc aactgcaatc aagaaagcct gtgcccctgt aggatgcagt
     5041 gaagaccttg gaagagacag cacaaaactg cacttgatga attttactgc aacatacaca
     5101 agacatcccc ctctcttacc aaatggcaaa gctctttgtc ataccacatc ttccccttta
     5161 ccaggagatg taaaggtttt atcagagaaa gcaatcctcc aatcatggac agacaatgag
     5221 agatccattc ctaatgatgg tacatgcttt caggaacaca gttcttatgg cagaaattct
     5281 ctggaagaca attcctgggt atttccaagt cctcctaaat caagtgagac agcatttggg
     5341 gaaactaaaa ctaaaacttt gcctttaccc aaccttccac cactgcatta cttggatcaa
     5401 cataatcaga actgccttta taagaattag ggatcctaat caacctctgg attacaaaat
     5461 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     5521 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     5581 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     5641 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     5701 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     5761 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     5821 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     5881 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     5941 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     6001 gatctccctt tgggccgcct ccccgcctga gatcctttaa gaccaatgac ttacaaggca
     6061 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     6121 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     6181 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     6241 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     6301 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     6361 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggcccgg gttaattaag
     6421 gaaagggcta gatcattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt
     6481 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     6541 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     6601 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     6661 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     6721 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     6781 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     6841 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca
     6901 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     6961 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     7021 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     7081 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     7141 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     7201 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     7261 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     7321 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     7381 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     7441 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     7501 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     7561 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     7621 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     7681 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     7741 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     7801 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     7861 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     7921 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     7981 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     8041 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     8101 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     8161 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     8221 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     8281 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     8341 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     8401 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     8461 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc aagctcatgg ctgactaatt
     8521 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     8581 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc ccgtggcacg acaggtttcc
     8641 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     8701 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     8761 acaatttcac acaggaaaca gctatgacat gattacgaat ttcacaaata aagcattttt
     8821 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     8881 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac
     8941 cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc
     9001 attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagt
//
