LOCUS       Exported                9604 bp ds-DNA     circular SYN 06-SEP-2023
DEFINITION  stably express GFP-tagged NDP52 for CID induced mitophagy.
ACCESSION   .
VERSION     .
KEYWORDS    pHAGE-FKBP-GFP-NDP52
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9604)
  AUTHORS   Vargas JNS, Wang C, Bunker E, Hao L, Maric D, Schiavo G, Randow F, 
            Youle RJ
  TITLE     Spatiotemporal Control of ULK1 Activation by NDP52 and TBK1 during 
            Selective Autophagy.
  JOURNAL   Mol Cell. 2019 Apr 18;74(2):347-362.e6. doi: 
            10.1016/j.molcel.2019.02.010. Epub 2019 Mar 7.
  PUBMED    30853401
REFERENCE   2  (bases 1 to 9604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Sep 11, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9604
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     1948..1974
                     /label=SMP3608
     misc_feature    2062..2179
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2180..2201
                     /label=SMP2956
     primer_bind     2191..2210
                     /label=SMP2937
     primer_bind     2194..2214
                     /label=SMP2954
     enhancer        2231..2534
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(2376..2397)
                     /label=SMP1803
     promoter        2535..2738
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2564..2579)
                     /label=SMP2619
     primer_bind     2643..2664
                     /label=SMP2400
     primer_bind     2646..2665
                     /label=EGFP-N-for
     primer_bind     2683..2701
                     /label=SMP443
     primer_bind     2689..2709
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2702..2718
                     /label=IRES-for
     primer_bind     2717..2737
                     /label=SMP736
     primer_bind     2728..2747
                     /label=pTRE-for
     primer_bind     2735..2759
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     2735..2755
                     /label=SMP2952
     primer_bind     2735..2752
                     /label=CEP4-for
     primer_bind     2789..2805
                     /label=TET-CMV-for
     regulatory      2821..2830
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2827..3150
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     primer_bind     complement(3083..3094)
                     /label=SMP296
     primer_bind     3107..3126
                     /label=SMP3313
     CDS             3157..3477
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             3496..4209
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     3496..3513
                     /label=SMP2824
     primer_bind     complement(3496..3513)
                     /label=SMP3729
     primer_bind     3499..3516
                     /label=SMP2016
     primer_bind     complement(3535..3551)
                     /label=PEN1-737R
     primer_bind     complement(3538..3559)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3540..3560)
                     /label=SMP919
     primer_bind     complement(3555..3573)
                     /label=SMP2564
     primer_bind     complement(3566..3586)
                     /label=SMP2562
     primer_bind     complement(3568..3586)
                     /label=EGFP-N-R+33
     primer_bind     complement(3601..3619)
                     /label=EGFP-N-rev
     primer_bind     complement(3609..3628)
                     /label=SMP2433
     primer_bind     complement(3624..3642)
                     /label=SMP3388
     primer_bind     3667..3687
                     /label=SMP2316
     primer_bind     3741..3760
                     /label=SMP1001
     primer_bind     3787..3806
                     /label=SMP1325
     primer_bind     complement(3797..3817)
                     /label=SMP2969
     primer_bind     complement(3799..3818)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(3854..3874)
                     /label=SMP2971
     primer_bind     complement(3857..3873)
                     /label=SMP2578
     primer_bind     complement(3887..3900)
                     /label=SMP2969
     primer_bind     3900..3918
                     /label=SMP444
     primer_bind     3910..3934
                     /label=SMP1666
     primer_bind     3964..3981
                     /label=SMP3040
     primer_bind     3964..3981
                     /label=SMP3104
     primer_bind     3969..3988
                     /label=SMP1330
     primer_bind     3995..4012
                     /label=SMP4080
     primer_bind     3996..4015
                     /label=SMP437
     primer_bind     complement(3996..4015)
                     /label=SMP471
     primer_bind     4034..4051
                     /label=SMP3892
     primer_bind     4047..4066
                     /label=SMP364
     primer_bind     4096..4128
                     /label=SMP295
     primer_bind     complement(4096..4128)
                     /label=SMP296
     primer_bind     4119..4137
                     /label=EGFP-C-F-31
     primer_bind     4128..4144
                     /label=EGFP-for
     primer_bind     4134..4152
                     /label=SMP564
     primer_bind     4146..4167
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4150..4167
                     /label=EGFP-C-for
     primer_bind     4182..4219
                     /label=SMP2241
     primer_bind     complement(4182..4200)
                     /label=SMP2415
     primer_bind     complement(4189..4209)
                     /label=SMP2429
     primer_bind     4189..4209
                     /label=SMP3728
     primer_bind     complement(4189..4208)
                     /label=SMP2596
     primer_bind     complement(4191..4219)
                     /label=SMP4373
     primer_bind     complement(4207..4233)
                     /label=SMP884
     primer_bind     complement(4213..4233)
                     /label=SMP1201
     primer_bind     complement(4231..4254)
                     /label=SMP1053
     misc_feature    4235..4274
                     /label=primer fw
     CDS             4255..4611
                     /codon_start=1
                     /label=OPTN (2-119)
                     /translation="SHQPLSCLTEKEDSPSESTGNGPPHLAHPNLDTFTPEELLQQMKE
                     LLTENHQLKEAMKLNNQAMKGRFEELSAWTEKQKEERQFFEIQSKEAKERLMALSHENE
                     KLKEELGKLKGKSE"
     primer_bind     4255..4267
                     /label=Vector.FOR
     primer_bind     complement(4383..4417)
                     /label=E50K R
     primer_bind     4383..4417
                     /label=E50K F
     primer_bind     complement(4585..4608)
                     /label=IVM_short_Rv
     primer_bind     4590..4629
                     /label=OPTN_STOP-2-119_IVM_Fw
     primer_bind     complement(4590..4629)
                     /label=OPTN_STOP_2-119_IVM_Rv
     primer_bind     4609..4627
                     /label=IVM_short_Fw
     primer_bind     complement(4763..4803)
                     /label=S173D R
     primer_bind     4763..4803
                     /label=S177D F
     primer_bind     complement(5644..5668)
                     /label=SMP1210
     primer_bind     complement(5649..5694)
                     /label=S473D R
     primer_bind     5649..5694
                     /label=S473D F
     misc_feature    5669..5671
                     /label=Feature 17
     misc_feature    5963..6006
                     /label=primer rev
     misc_feature    5994..6582
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6047..6067)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     complement(6049..6072)
                     /label=SMP3898
     primer_bind     complement(6050..6070)
                     /label=SMP1896
     primer_bind     complement(6061..6080)
                     /label=WHV-5R
     CDS             complement(6465..6476)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6657..6890
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7002..7020)
                     /label=QE-for
     primer_bind     complement(7009..7027)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     7094..7114
                     /label=SMP646
     primer_bind     complement(7094..7114)
                     /label=SMP654
     promoter        7095..7199
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7200..8060
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7245..7264)
                     /label=AmpStart
     primer_bind     complement(7418..7437)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     7991..8010
                     /label=AmpStop
     rep_origin      8231..8819
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8611..8628
                     /label=SMP4077
     primer_bind     8720..8739
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8973..8990
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     9082..9101
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(9105..9116)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     9198..9215
                     /label=SMP2212
     primer_bind     complement(9200..9217)
                     /label=SMP4078
     protein_bind    9230..9251
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9266..9296
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     9277..9296
                     /label=pUCM13-rev-157
     protein_bind    9304..9320
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9309..9331
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9311..9331
                     /label=SMP547
     primer_bind     9311..9330
                     /label=LacOp-for
     primer_bind     9323..9342
                     /label=SMP993
     primer_bind     9328..9344
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9328..9344
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9381..9402)
                     /label=SMP3893
     primer_bind     9394..9413
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttaattcaca
     2041 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     2101 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     2161 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggactag
     2221 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2281 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2341 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2401 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2461 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2521 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2581 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2641 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     2701 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     2761 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccggcgg ccgcggtacc
     2821 gccaccatgg gagtgcaggt ggaaaccatc tccccaggag acgggcgcac cttccccaag
     2881 cgcggccaga cctgcgtggt gcactacacc gggatgcttg aagatggaaa gaaatttgat
     2941 tcctcccggg acagaaacaa gccctttaag tttatgctag gcaagcagga ggtgatccga
     3001 ggctgggaag aaggggttgc ccagatgagt gtgggtcaga gagccaaact gactatatct
     3061 ccagattatg cctatggtgc cactgggcac ccaggcatca tcccaccaca tgccactctc
     3121 gtcttcgatg tggagcttct aaaactggaa actagaggcg tgcaggtcga gaccatcagc
     3181 cccggcgacg gccgcacctt tcccaagaga ggccagactt gcgtggtcca ctacaccggc
     3241 atgctggagg acggcaagaa gttcgacagc agccgcgacc gcaacaagcc cttcaagttc
     3301 atgctgggca aacaggaagt gatccgcggc tgggaggaag gcgtggctca gatgagcgtg
     3361 gggcagcggg ccaagctgac catcagcccc gactatgcct acggcgccac cggccacccc
     3421 ggcatcatcc ccccccacgc caccctggtg ttcgacgtgg agctgctgaa gctggagact
     3481 agtaccggtt ctagagtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     3541 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     3601 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     3661 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     3721 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     3781 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     3841 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     3901 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     3961 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     4021 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     4081 cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc
     4141 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     4201 ctgtacaagt ccggactcag atctcgagct caagcttcga attctgcagt cgactcccat
     4261 caacctctca gctgcctcac tgaaaaggag gacagcccca gtgaaagcac aggaaatgga
     4321 cccccccacc tggcccaccc aaacctggac acgtttaccc cggaggagct gctgcagcag
     4381 atgaaagagc tcctgaccga gaaccaccag ctgaaagaag ccatgaagct aaataatcaa
     4441 gccatgaaag ggagatttga ggagctttcg gcctggacag agaaacagaa ggaagaacgc
     4501 cagttttttg agatacagag caaagaagca aaagagcgtc taatggcctt gagtcatgag
     4561 aatgagaaat tgaaggaaga gcttggaaaa ctaaaaggga aatcagaata ggtcatctga
     4621 ggaccccact gatgactcca ggcttcccag ggccgaagcg gagcaggaaa aggaccagct
     4681 caggacccag gtggtgaggc tacaagcaga gaaggcagac ctgttgggca tcgtgtctga
     4741 actgcagctc aagctgaact ccagcggctc ctcagaagat tcctttgttg aaattaggat
     4801 ggctgaagga gaagcagaag ggtcagtaaa agaaatcaag catagtcctg ggcccacgag
     4861 aacagtctcc actggcacgg cattgtctaa atataggagc agatctgcag atggggccaa
     4921 gaattacttc gaacatgagg agttaactgt gagccagctc ctgctgtgcc taagggaagg
     4981 gaatcagaag gtggagagac ttgaagttgc actcaaggag gccaaagaaa gagtttcaga
     5041 ttttgaaaag aaaacaagta atcgttctga gattgaaacc cagacagagg ggagcacaga
     5101 gaaagagaat gatgaagaga aaggcccgga gactgttgga agcgaagtgg aagcactgaa
     5161 cctccaggtg acatctctgt ttaaggagct tcaagaggct catacaaaac tcagcgaagc
     5221 tgagctaatg aagaagagac ttcaagaaaa gtgtcaggcc cttgaaagga aaaattctgc
     5281 aattccatca gagttgaatg aaaagcaaga gcttgtttat actaacaaaa agttagagct
     5341 acaagtggaa agcatgctat cagaaatcaa aatggaacag gctaaaacag aggatgaaaa
     5401 gtccaaatta actgtgctac agatgacaca caacaagctt cttcaagaac ataataatgc
     5461 attgaaaaca attgaggaac taacaagaaa agagtcagaa aaagtggaca gggcagtgct
     5521 gaaggaactg agtgaaaaac tggaactggc agagaaggct ctggcttcca aacagctgca
     5581 aatggatgaa atgaagcaaa ccattgccaa gcaggaagag gacctggaaa ccatgaccat
     5641 cctcagggct cagatggaag tttactgttc tgattttcat gctgaaagag cagcgagaga
     5701 gaaaattcat gaggaaaagg agcaactggc attgcagctg gcagttctgc tgaaagagaa
     5761 tgatgctttc gaagacggag gcaggcagtc cttgatggag atgcagagtc gtcatggggc
     5821 gagaacaagt gactctgacc agcaggctta ccttgttcaa agaggagctg aggacaggga
     5881 ctggcggcaa cagcggaata ttccgattca ttcctgcccc aagtgtggag aggttctgcc
     5941 tgacatagac acgttacaga ttcacgtgat ggattgcatc atttaaggat cctaatcaac
     6001 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     6061 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     6121 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     6181 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     6241 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     6301 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     6361 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     6421 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     6481 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     6541 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgagatc ctttaagacc
     6601 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     6661 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     6721 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     6781 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     6841 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     6901 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     6961 gcccgggtta attaaggaaa gggctagatc attcttgaag acgaaagggc ctcgtgatac
     7021 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     7081 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     7141 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     7201 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     7261 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     7321 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     7381 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     7441 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     7501 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     7561 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     7621 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     7681 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     7741 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     7801 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     7861 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     7921 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     7981 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     8041 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     8101 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     8161 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     8221 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     8281 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     8341 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     8401 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     8461 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     8521 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     8581 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     8641 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     8701 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     8761 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     8821 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     8881 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     8941 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     9001 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc
     9061 tcatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     9121 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt
     9181 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     9241 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     9301 gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca
     9361 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     9421 cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca
     9481 ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt
     9541 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
     9601 tagt
//
