LOCUS       Exported                8058 bp ds-DNA     circular SYN 21-AUG-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8058)
  AUTHORS   eliasa55
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Sep 11, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8058
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      102..557
                     /label=M13 origin
     rep_origin      119..425
                     /label=F1 ori
     CDS             887..1546
                     /label=AmpR
     rep_origin      1644..2326
                     /label=ColE1 origin
     misc_feature    4528..4549
                     /label=148 Pph_seqF
     misc_feature    4612..5274
                     /label=GST
     primer_bind     5230..5249
                     /label=GST-Fw
     misc_feature    5275..5286
                     /label=GST
                     /label=GST(1)
     primer_bind     5301..5350
                     /label=Fragment.FOR
     misc_feature    5302..5322
                     /label=TEV cleavage ENLYFQ^G
     misc_feature    5329..7518
                     /label=TBK1-CDS
     primer_bind     5920..5939
                     /label=TBK1 Seq Fw-1
     primer_bind     6539..6558
                     /label=TBK1 Seq Fw-2
     primer_bind     6759..6778
                     /label=TBK1 Seq Fw-3
     primer_bind     complement(7494..7542)
                     /label=Fragment.REV
     misc_feature    7553..7573
                     /label=4 SV40_seqR
     polyA_signal    complement(7587..7778)
                     /label=SV40 late polyA
     primer_bind     complement(7589..7620)
                     /label=SV40 Rv - SMP0004
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
      421 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
      481 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
      541 attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      601 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      661 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      721 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      781 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      841 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      901 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
      961 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     1021 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     1081 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     1141 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     1201 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     1261 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     1321 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     1381 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     1441 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1501 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1561 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1621 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1681 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1741 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1801 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1861 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1921 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1981 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2041 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2101 acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2161 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2221 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2281 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2341 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     2401 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     2461 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2521 tctgtgcggt atttcacacc gcagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acctgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg ccctggcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcct acgtacccgt agtggctatg gcagggcttg ccgccccgac
     4021 gttggctgcg agccctgggc cttcacccga acttgggggt tggggtgggg aaaaggaaga
     4081 aacgcgggcg tattggtccc aatggggtct cggtggggta tcgacagagt gccagccctg
     4141 ggaccgaacc ccgcgtttat gaacaaacga cccaacaccc gtgcgtttta ttctgtcttt
     4201 ttattgccgt catagcgcgg gttccttccg gtattgtctc cttccgtgtt tcagttagcc
     4261 tcccccatct cccggtaccg catgctatgc atcagctgct agcaccatgg ctcgagatcc
     4321 cgggtgatca agtcttcgtc gagtgattgt aaataaaatg taatttacag tatagtattt
     4381 taattaatat acaaatgatt tgataataat tcttatttaa ctataatata ttgtgttggg
     4441 ttgaattaaa ggtccgtata ctccggaata ttaatagatc atggagataa ttaaaatgat
     4501 aaccatctcg caaataaata agtattttac tgttttcgta acagttttgt aataaaaaaa
     4561 cctataaata ttccggatta ttcataccgt cccaccatcg ggcgcggatc catggcatcc
     4621 cctatactag gttattggaa aattaagggc cttgtgcaac ccactcgact tcttttggaa
     4681 tatcttgaag aaaaatatga agagcatttg tatgagcgcg atgaaggtga taaatggcga
     4741 aacaaaaagt ttgaattggg tttggagttt cccaatcttc cttattatat tgatggtgat
     4801 gttaaattaa cacagtctat ggccatcata cgttatatag ctgacaagca caacatgttg
     4861 ggtggttgtc caaaagagcg tgcagagatt tcaatgcttg aaggagcggt tttggatatt
     4921 agatacggtg tttcgagaat tgcatatagt aaagactttg aaactctcaa agttgatttt
     4981 cttagcaagc tacctgaaat gctgaaaatg ttcgaagatc gtttatgtca taaaacatat
     5041 ttaaatggtg atcatgtaac ccatcctgac ttcatgttgt atgacgctct tgatgttgtt
     5101 ttatacatgg acccaatgtg cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt
     5161 attgaagcta tcccacaaat tgataagtac ttgaaatcca gcaagtatat agcatggcct
     5221 ttgcagggct ggcaagccac gtttggtggt ggcgaccatc ctccaaaatc ggatctggtt
     5281 ccgcgtggat caggatcagg agagaattta tactttcagg gagcgcgcat gcaatccaca
     5341 tccaaccacc tctggctcct ctcggacatt ctcggacaag gcgctaccgc taacgtgttc
     5401 aggggaaggc acaagaagac cggtgacctg ttcgccatca aggtgttcaa caacatctca
     5461 ttcctgcgcc ctgtggacgt ccagatgcgt gagttcgaag tgctgaagaa gctgaaccac
     5521 aagaacatcg tcaagctgtt cgccatcgag gaagaaacca ctacccgcca caaggtgctg
     5581 atcatggagt tctgcccctg cggttccctg tacactgtcc tggaagagcc ttccaacgct
     5641 tacggtctgc ccgagagcga gttcctgatc gtcctgcgtg acgtggtcgg tggcatgaac
     5701 cacctgaggg aaaacggcat cgtgcacaga gacatcaagc caggaaacat catgcgcgtg
     5761 atcggcgagg acggacagag cgtctacaag ctgaccgact tcggcgctgc cagagagctg
     5821 gaagacgacg aacagttcgt ctctctgtac ggaactgaag agtacctgca ccctgacatg
     5881 tacgagaggg ccgtgctgag aaaggaccac cagaagaagt acggtgctac cgtggacctg
     5941 tggtctatcg gcgtcacctt ctaccacgct gccactggat cactgccttt ccgccccttc
     6001 gaaggtcctc gccgtaacaa ggaggtcatg tacaagatca tcactggcaa gccctctggc
     6061 gccatctcag gagtccagaa ggctgaaaac ggtcccatcg actggagcgg cgacatgcca
     6121 gtgagctgct ctctgtcccg cggtctgcaa gtgctgctga ccccagtcct ggccaacatc
     6181 ctggaagctg accaggagaa gtgctggggt ttcgaccagt tcttcgctga aacctccgac
     6241 atcctgcaca gaatggtcat ccacgtcttc agcctgcaac agatgactgc ccacaagatt
     6301 tacatccact cttacaacac tgctactatc ttccacgaac tggtctacaa gcagaccaag
     6361 atcatctcca gcaaccagga actgatctac gagggaagga gactggtgct ggagcccggt
     6421 cgcctggctc agcacttccc aaagactacc gaagagaacc ctatcttcgt ggtgtcccgc
     6481 gagccactga acactatcgg cctgatctac gagaagatca gcctgcccaa ggtccaccca
     6541 cgttacgacc tggacggcga cgctagcatg gctaaggcca tcaccggagt ggtctgctac
     6601 gcttgccgca tcgcttcaac tctgctgctg taccaggaac tgatgcgcaa gggaatccgt
     6661 tggctgatcg agctgatcaa ggacgactac aacgaaactg tgcacaagaa gaccgaggtg
     6721 gtcatcactc tggacttctg catccgtaac atcgaaaaga ccgtgaaggt ctacgagaag
     6781 ctgatgaaga tcaacctgga ggctgccgaa ctgggagaga tcagcgacat ccacactaag
     6841 ctgctgcgcc tgtcttcatc ccagggcact atcgaaacct ccctgcaaga catcgactcc
     6901 cgtctgagcc caggaggttc actggctgac gcttgggctc accaggaagg cacccaccct
     6961 aaggacagga acgtggagaa gctgcaagtc ctgctgaact gcatgactga aatctactac
     7021 cagttcaaga aggacaaggc cgagcgccgt ctggcttaca acgaagagca gatccacaag
     7081 ttcgacaagc agaagctgta ctaccacgcc accaaggcta tgactcactt caccgacgaa
     7141 tgcgtcaaga agtacgaggc tttcctgaac aagagcgaag agtggatcag gaagatgctg
     7201 cacctgagaa agcagctgct gtccctgacc aaccagtgct tcgacatcga agaggaagtg
     7261 agcaagtacc aggagtacac caacgagctg caagagactc tgcctcagaa gatgttcact
     7321 gccagctctg gtatcaagca cactatgacc ccaatctacc cttcatccaa caccctggtg
     7381 gaaatgactc tgggtatgaa gaagctgaag gaggaaatgg agggcgtggt caaggaactg
     7441 gctgagaaca accacatctt ggaaagattc ggtagcctga cgatggatgg tggactgaga
     7501 aacgtggact gtctgtaaaa gcttgtcgag aagtactaga ggatcataat cagccatacc
     7561 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     7621 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     7681 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     7741 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctgatcact gcttgagcct
     7801 aggagatccg aaccagataa gtgaaatcta gttccaaact attttgtcat ttttaatttt
     7861 cgtattagct tacgacgcta cacccagttc ccatctattt tgtcactctt ccctaaataa
     7921 tccttaaaaa ctccatttcc acccctccca gttcccaact attttgtccg cccacagcgg
     7981 ggcatttttc ttcctgttat gtttttaatc aaacatcctg ccaactccat gtgacaaacc
     8041 gtcatcttcg gctacttt
//
