LOCUS       pCMV-T7-PCV2-nCas9-deadEcKlenow_(JO520)        9909 bp    DNA     circular SYN 24-NOV-2023
DEFINITION  .
ACCESSION
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
COMMENT     Annotated with pLannotate v1.2.0.
FEATURES             Location/Qualifiers
     misc_feature    complement(173..792)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /X-Transferred_From="pBR322 origin"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pCAG-hCas9-nls-3XFLAG (SQT817),
                     pCAG-hCas9-nls-3XFLAG delta NcoI (BPK742), pCMV-T7-Cas9
                     (MLM3613), pCMV-T7-Cas9 delta NcoI"
                     /Transferred_Similarity="100.00%"
                     /label="pBR322 origin"
                     /note="Geneious type: origin of replication"
     CDS             complement(947..1807)
                     /gene="Ampicillin"
                     /Color="#777777"
                     /modified_by="ben"
                     /Original_Translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVL
                     GNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKV
                     DDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADL
                     RLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAK
                     AILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLS
                     KDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRY
                     DEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKM
                     DGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIE
                     KILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFD
                     KNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNR
                     KVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDI
                     LEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDK
                     QSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSP
                     AIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIK
                     ELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFL
                     KDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAER
                     GGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLV
                     SDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKM
                     IAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRD
                     FATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSP
                     TVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLI
                     IKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNE
                     QKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIH
                     LFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDG
                     SPKKKRKVSSDYKDHDGDYKDHDIDYKDDDDKAAG*"
                     /label="Ampicillin"
     promoter        complement(1808..1912)
                     /X-Transferred_From="JDS246; pCMV-NLS-hTtAgo-NLS-3xFLAG;
                     pLV-CMVEI.EGFPDS-iNpLTRSEBBA6_0693; pLVX-TetOne-puro;
                     pMLM3705; pMLM3705 annotation mess"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pCMV-T7-Cas9 (MLM3613), pCMV-T7-Cas9
                     delta NcoI"
                     /Transferred_Similarity="100.00%"
                     /label="AmpR promoter"
     promoter        2196..2561
                     /SerialCloner_Color="&h03C000"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /X-Transferred_From="3.
                     pCAG-nls-Csy4-FokI-T2A-dCas9-recoded (C-terminal fusion) -
                     linker variants; 6.pCAG-nls-FokI-Csy4-T2A-Cas9-recoded
                     (N-terminal fusion) -linker variants; JDS246;
                     pCAG-Csy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK376); pCAG-Csy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64
                     recoded (BPK376frameshift);
                     pCAG-Csy4-P2A-dCas9-NLS-FLAG-VP64 recoded;
                     pCAG-Csy4-T2A-Cas9-VP64 recoded (BPK1);
                     pCAG-Csy4-T2A-Cas9-VP64 recoded (BPK2a);
                     pCAG-Csy4-T2A-Cas9-recoded (SQT834);
                     pCAG-Csy4-T2A-FokI-dCas9-recoded-nls (JAF1484);
                     pCAG-Csy4-T2A-KRAB-dCas9-recoded-nlsFLAG (NW9);
                     pCAG-Csy4-T2A-NLS-FLAG-NLS-VP64-dCas9-recoded (making
                     p125); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-KRAB recoded
                     (BPK353); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-VP64 recoded
                     (BPK326); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-VP64 recoded NO
                     CTERM FLAG;
                     pCAG-Csy4-T2A-VP64-NLS/FLAG/NLS-dCas9-recoded-nlsFLAG
                     (BPK328); pCAG-Csy4-T2A-VP64-dCas9 linker variant BbsI
                     cassette; pCAG-Csy4-T2A-VP64-dCas9-recoded-nlsFLAG
                     (BPK285); pCAG-Csy4-T2A-VP64-dCas9-recoded-nlsFLAG
                     (BPK286); pCAG-Csy4-T2A-dCas9-KRAB recoded (NW8);
                     pCAG-Csy4-T2A-dCas9-VP64 recoded (BPK3);
                     pCAG-Csy4-T2A-dCas9-VP64 recoded NO FLAG (BPK373 or 374?);
                     pCAG-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64;
                     pCAG-NLS-FLAG-NLS-dCas9-VP64 recoded;
                     pCAG-NLS-FLAG-VP64-dCas9-recoded;
                     pCAG-NLS-VP64-dCas9-recoded; pCAG-NLS-dCas9-NLS-3xFLAG
                     recoded; pCAG-NLS-dCas9-VP64 recoded; pCAG-NLS-dCas9-VP64
                     recoded CopyXXXXX;
                     pCAG-NLS-hCsy4-P2A-NLS-FLAG-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-NLS-hTtAgo (BPK633); pCAG-NLS-hTtAgo-NLS-3xFLAG
                     (BPK573); pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64;
                     pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64 D478A/D546A;
                     pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64 D478N/D546N;
                     pCAG-VP64-NLS-FLAG-NLS-dCas9-recoded-NLS-FLAG (BPK329);
                     pCAG-VP64-NLS-hTtAgo; pCAG-VP64-dCas9-recod linker variant
                     BbsI cassette; pCAG-VP64-dCas9-recoded GGGGSx5 linker;
                     pCAG-VP64-dCas9-recoded-NLS-FLAG (BPK278);
                     pCAG-VP64-hCsy4-NLS-P2A-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-dCas9-VP64;
                     pCAG-hCsy4-NLS-P2A-NLS-FLAG-NLS-hdCas9-NLS-FLAG-VP64
                     (BPK553);
                     pCAG-hCsy4-NLS-VP64-P2A-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-3xFLAG-KRAB
                     (BPK550); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64
                     delta175-307 (BPK544);
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded;
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK447);
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64-GG recoded
                     (BPK531); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-KRAB
                     (BPK454); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-VP64
                     recoded (BPK453); pCAG-hCsy4-P2A-NLS-dCas9-NLS-FLAG-VP64
                     recoded (BPK546); pCAG-hCsy4-P2A-NLS-dCas9-NLS-KRAB
                     (BPK548); pCAG-hCsy4-P2A-dCas9-NLS-FLAG-VP64 recoded
                     (BPK422); pCAG-hCsy4-P2A-dCas9-NLS-KRAB (BPK439);
                     pCAG-hCsy4-P2A-dCas9-NLS-VP64 recoded (BPK450);
                     pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK428); pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-KRAB
                     (BPK444); pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-VP64
                     recoded (BPK437); pCAG-hCsy4-T2A-dCas9-NLS-FLAG-VP64
                     recoded (BPK426); pCAG-hCsy4-T2A-dCas9-NLS-KRAB (BPK442);
                     pCAG-hCsy4-T2A-dCas9-NLS-VP64 recoded (BPK435);
                     pCAG-hCsy4-T2A-nls-hFokI-dhCas9-nls-3XFLAG (SQT1601);
                     pCAG-hTtAgo; pCAG-hTtAgo-NLS; pCAG-hTtAgo-NLS-3xFLAG;
                     pCAG-nls-FokI-Csy4-T2A-dCas9-recoded (N-terminal fusion
                     CK1891); pCMV-NLS-hTtAgo-NLS-3xFLAG; pLenti-CAG-Puro-DEST;
                     pMLM3705; pMLM3705 annotation mess"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pCMV-T7-Cas9 (MLM3613), pCMV-T7-Cas9
                     delta NcoI"
                     /Transferred_Similarity="100.00%"
                     /modified_by="lha16"
                     /label="CMV Enhancer"
     promoter        complement(2245..2273)
                     /SerialCloner_Color="&hF08080"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""AmpR_promoter"" -
                     translation=""MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     -
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     -
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     - DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWM \"
                     /X-Transferred_From="3.
                     pCAG-nls-Csy4-FokI-T2A-dCas9-recoded (C-terminal fusion) -
                     linker variants; 6.pCAG-nls-FokI-Csy4-T2A-Cas9-recoded
                     (N-terminal fusion) -linker variants; JDS246;
                     pCAG-Csy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK376); pCAG-Csy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64
                     recoded (BPK376frameshift);
                     pCAG-Csy4-P2A-dCas9-NLS-FLAG-VP64 recoded;
                     pCAG-Csy4-T2A-Cas9-VP64 recoded (BPK1);
                     pCAG-Csy4-T2A-Cas9-VP64 recoded (BPK2a);
                     pCAG-Csy4-T2A-Cas9-recoded (SQT834);
                     pCAG-Csy4-T2A-FokI-dCas9-recoded-nls (JAF1484);
                     pCAG-Csy4-T2A-KRAB-dCas9-recoded-nlsFLAG (NW9);
                     pCAG-Csy4-T2A-NLS-FLAG-NLS-VP64-dCas9-recoded (making
                     p125); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-KRAB recoded
                     (BPK353); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-VP64 recoded
                     (BPK326); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-VP64 recoded NO
                     CTERM FLAG;
                     pCAG-Csy4-T2A-VP64-NLS/FLAG/NLS-dCas9-recoded-nlsFLAG
                     (BPK328); pCAG-Csy4-T2A-VP64-dCas9 linker variant BbsI
                     cassette; pCAG-Csy4-T2A-VP64-dCas9-recoded-nlsFLAG
                     (BPK285); pCAG-Csy4-T2A-VP64-dCas9-recoded-nlsFLAG
                     (BPK286); pCAG-Csy4-T2A-dCas9-KRAB recoded (NW8);
                     pCAG-Csy4-T2A-dCas9-VP64 recoded (BPK3);
                     pCAG-Csy4-T2A-dCas9-VP64 recoded NO FLAG (BPK373 or 374?);
                     pCAG-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64;
                     pCAG-NLS-FLAG-NLS-dCas9-VP64 recoded;
                     pCAG-NLS-FLAG-VP64-dCas9-recoded;
                     pCAG-NLS-VP64-dCas9-recoded; pCAG-NLS-dCas9-NLS-3xFLAG
                     recoded; pCAG-NLS-dCas9-VP64 recoded; pCAG-NLS-dCas9-VP64
                     recoded CopyXXXXX;
                     pCAG-NLS-hCsy4-P2A-NLS-FLAG-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-NLS-hTtAgo (BPK633); pCAG-NLS-hTtAgo-NLS-3xFLAG
                     (BPK573); pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64;
                     pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64 D478A/D546A;
                     pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64 D478N/D546N;
                     pCAG-VP64-NLS-FLAG-NLS-dCas9-recoded-NLS-FLAG (BPK329);
                     pCAG-VP64-NLS-hTtAgo; pCAG-VP64-dCas9-recod linker variant
                     BbsI cassette; pCAG-VP64-dCas9-recoded GGGGSx5 linker;
                     pCAG-VP64-dCas9-recoded-NLS-FLAG (BPK278);
                     pCAG-VP64-hCsy4-NLS-P2A-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-dCas9-VP64;
                     pCAG-hCsy4-NLS-P2A-NLS-FLAG-NLS-hdCas9-NLS-FLAG-VP64
                     (BPK553);
                     pCAG-hCsy4-NLS-VP64-P2A-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-3xFLAG-KRAB
                     (BPK550); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64
                     delta175-307 (BPK544);
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded;
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK447);
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64-GG recoded
                     (BPK531); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-KRAB
                     (BPK454); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-VP64
                     recoded (BPK453); pCAG-hCsy4-P2A-NLS-dCas9-NLS-FLAG-VP64
                     recoded (BPK546); pCAG-hCsy4-P2A-NLS-dCas9-NLS-KRAB
                     (BPK548); pCAG-hCsy4-P2A-dCas9-NLS-FLAG-VP64 recoded
                     (BPK422); pCAG-hCsy4-P2A-dCas9-NLS-KRAB (BPK439);
                     pCAG-hCsy4-P2A-dCas9-NLS-VP64 recoded (BPK450);
                     pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK428); pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-KRAB
                     (BPK444); pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-VP64
                     recoded (BPK437); pCAG-hCsy4-T2A-dCas9-NLS-FLAG-VP64
                     recoded (BPK426); pCAG-hCsy4-T2A-dCas9-NLS-KRAB (BPK442);
                     pCAG-hCsy4-T2A-dCas9-NLS-VP64 recoded (BPK435);
                     pCAG-hCsy4-T2A-nls-hFokI-dhCas9-nls-3XFLAG (SQT1601);
                     pCAG-hTtAgo; pCAG-hTtAgo-NLS; pCAG-hTtAgo-NLS-3xFLAG;
                     pCAG-nls-FokI-Csy4-T2A-dCas9-recoded (N-terminal fusion
                     CK1891); pCMV-NLS-hTtAgo-NLS-3xFLAG; pLenti-CAG-Puro-DEST;
                     pMLM3705; pMLM3705 annotation mess; pcDNA5/FRT"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pCMV-T7-Cas9 (MLM3613), pCMV-T7-Cas9
                     delta NcoI"
                     /Transferred_Similarity="100.00%"
                     /label="AmpR_promoter"
     promoter        2262..2549
                     /SerialCloner_Color="&hDC143C"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""CAG_enhancer"" - 84..371 \"
                     /X-Transferred_From="3.
                     pCAG-nls-Csy4-FokI-T2A-dCas9-recoded (C-terminal fusion) -
                     linker variants; 6.pCAG-nls-FokI-Csy4-T2A-Cas9-recoded
                     (N-terminal fusion) -linker variants; JDS246;
                     pCAG-AttB-Csy4-VEGFA1-3 (BPK318); pCAG-CFP;
                     pCAG-Csy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK376); pCAG-Csy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64
                     recoded (BPK376frameshift);
                     pCAG-Csy4-P2A-dCas9-NLS-FLAG-VP64 recoded;
                     pCAG-Csy4-T2A-Cas9-VP64 recoded (BPK1);
                     pCAG-Csy4-T2A-Cas9-VP64 recoded (BPK2a);
                     pCAG-Csy4-T2A-Cas9-recoded (SQT834);
                     pCAG-Csy4-T2A-FokI-dCas9-recoded-nls (JAF1484);
                     pCAG-Csy4-T2A-KRAB-dCas9-recoded-nlsFLAG (NW9);
                     pCAG-Csy4-T2A-NLS-FLAG-NLS-VP64-dCas9-recoded (making
                     p125); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-KRAB recoded
                     (BPK353); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-VP64 recoded
                     (BPK326); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-VP64 recoded NO
                     CTERM FLAG;
                     pCAG-Csy4-T2A-VP64-NLS/FLAG/NLS-dCas9-recoded-nlsFLAG
                     (BPK328); pCAG-Csy4-T2A-VP64-dCas9 linker variant BbsI
                     cassette; pCAG-Csy4-T2A-VP64-dCas9-recoded-nlsFLAG
                     (BPK285); pCAG-Csy4-T2A-VP64-dCas9-recoded-nlsFLAG
                     (BPK286); pCAG-Csy4-T2A-dCas9-KRAB recoded (NW8);
                     pCAG-Csy4-T2A-dCas9-VP64 recoded (BPK3);
                     pCAG-Csy4-T2A-dCas9-VP64 recoded NO FLAG (BPK373 or 374?);
                     pCAG-Csy4site2-gRNA-Csy4site2 (SQT1468) + 1 site;
                     pCAG-Csy4site2-gRNA-Csy4site2 (SQT1468) + 4 sites;
                     pCAG-Csy4site2-gRNA-Csy4site2 BsaI;
                     pCAG-Csy4site2-gRNA-Csy4site2 BsaI cassette; pCAG-DEST
                     (BPK88); pCAG-DEST V2;
                     pCAG-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64;
                     pCAG-NLS-FLAG-NLS-dCas9-VP64 recoded;
                     pCAG-NLS-FLAG-VP64-dCas9-recoded;
                     pCAG-NLS-VP64-dCas9-recoded; pCAG-NLS-dCas9-NLS-3xFLAG
                     recoded; pCAG-NLS-dCas9-VP64 recoded; pCAG-NLS-dCas9-VP64
                     recoded CopyXXXXX;
                     pCAG-NLS-hCsy4-P2A-NLS-FLAG-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-NLS-hTtAgo (BPK633); pCAG-NLS-hTtAgo-NLS-3xFLAG
                     (BPK573); pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64;
                     pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64 D478A/D546A;
                     pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64 D478N/D546N;
                     pCAG-VP64-NLS-FLAG-NLS-dCas9-recoded-NLS-FLAG (BPK329);
                     pCAG-VP64-NLS-hTtAgo; pCAG-VP64-dCas9-recod linker variant
                     BbsI cassette; pCAG-VP64-dCas9-recoded GGGGSx5 linker;
                     pCAG-VP64-dCas9-recoded-NLS-FLAG (BPK278);
                     pCAG-VP64-hCsy4-NLS-P2A-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-dCas9-VP64;
                     pCAG-hCsy4-NLS-P2A-NLS-FLAG-NLS-hdCas9-NLS-FLAG-VP64
                     (BPK553);
                     pCAG-hCsy4-NLS-VP64-P2A-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-3xFLAG-KRAB
                     (BPK550); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64
                     delta175-307 (BPK544);
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded;
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK447);
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64-GG recoded
                     (BPK531); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-KRAB
                     (BPK454); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-VP64
                     recoded (BPK453); pCAG-hCsy4-P2A-NLS-dCas9-NLS-FLAG-VP64
                     recoded (BPK546); pCAG-hCsy4-P2A-NLS-dCas9-NLS-KRAB
                     (BPK548); pCAG-hCsy4-P2A-dCas9-NLS-FLAG-VP64 recoded
                     (BPK422); pCAG-hCsy4-P2A-dCas9-NLS-KRAB (BPK439);
                     pCAG-hCsy4-P2A-dCas9-NLS-VP64 recoded (BPK450);
                     pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK428); pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-KRAB
                     (BPK444); pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-VP64
                     recoded (BPK437); pCAG-hCsy4-T2A-dCas9-NLS-FLAG-VP64
                     recoded (BPK426); pCAG-hCsy4-T2A-dCas9-NLS-KRAB (BPK442);
                     pCAG-hCsy4-T2A-dCas9-NLS-VP64 recoded (BPK435);
                     pCAG-hCsy4-T2A-nls-hFokI-dhCas9-nls-3XFLAG (SQT1601);
                     pCAG-hTtAgo; pCAG-hTtAgo-NLS; pCAG-hTtAgo-NLS-3xFLAG;
                     pCAG-nls-FokI-Csy4-T2A-dCas9-recoded (N-terminal fusion
                     CK1891); pCMV-NLS-hTtAgo-NLS-3xFLAG; pLenti-CAG-Puro-DEST;
                     pMLM3705; pMLM3705 annotation mess"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pCMV-T7-Cas9 (MLM3613), pCMV-T7-Cas9
                     delta NcoI"
                     /Transferred_Similarity="100.00%"
                     /modified_by="ben"
                     /label="CAG_enhancer"
     promoter        2562..2786
                     /label="CMV_promoter"
     promoter        2807..2826
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /modified_by="lha16"
                     /label="T7 promoter"
     motif           2830..2842
                     /created_by="ben"
                     /label="Kozak"
     CDS             2839..3189
                     /created_by="KleinstiverLab8"
                     /Color="#ffbb33"
                     /modified_by="ben"
                     /label="PCV2 HUHe"
     CDS             3190..3261
                     /created_by="KleinstiverLab8"
                     /Color="#ff7400"
                     /modified_by="ben"
                     /label="GS-XTEN-GS linker"
     CDS             3259..7362
                     /product="nickase mutant of the Cas9 endonuclease from
                     theStreptococcus pyogenes Type II CRISPR/Cas system"
                     /note="generates RNA-guided single strand nicks in DNA
                     dueto the H840A mutation in the HNH catalytic domain"
                     /Original_Translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLG
                     NTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVD
                     DSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
                     LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKA
                     ILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSK
                     DTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYD
                     EHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMD
                     GTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
                     ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDK
                     NLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRK
                     VTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDIL
                     EDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQ
                     SGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPA
                     IKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKE
                     LGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLK
                     DDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERG
                     GLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVS
                     DFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMI
                     AKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDF
                     ATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPT
                     VAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLII
                     KLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQ
                     KQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHL
                     FTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
                     /modified_by="chenp"
                     /modified_by="kleinstivermac7"
                     /Color="#eeeeee"
                     /modified_by="ben"
                     /label="nSpCas9(H840A)"
     CDS             5776..5778
                     /created_by="ben"
                     /label="H840A"
     CDS             7363..7386
                     /Color="#ff7400"
                     /created_by="ben"
                     /label="GS linker"
     CDS             7387..7440
                     /created_by="chenp"
                     /Color="#6bba70"
                     /modified_by="ben"
                     /label="BPNLS"
     CDS             7441..7464
                     /Color="#ff7400"
                     /created_by="ben"
                     /label="GS linker"
     CDS             7465..9279
                     /created_by="joana"
                     /Color="#ee0077"
                     /modified_by="lha16"
                     /label="dEcKlenow(D355A/D357A/D705A/D882A)"
     CDS             8608..8610
                     /created_by="lha16"
                     /modified_by="lha16"
                     /label="D705A"
     CDS             9139..9141
                     /created_by="lha16"
                     /modified_by="lha16"
                     /label="D882A"
     CDS             9280..9291
                     /Color="#ff7400"
                     /created_by="ben"
                     /label="GS linker"
     CDS             9292..9345
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /Original_Translation="KRTADGSEFEPKKKRKV"
                     /Color="#6bba70"
                     /modified_by="ben"
                     /label="BPNLS"
     CDS             9346..9348
                     /Color="#000000"
                     /created_by="ben"
                     /label
     terminator      9397..9624
                     /label="bGH_PA_terminator"
     terminator      9883..105
                     /SerialCloner_Color="&hF08080"
                     /SerialCloner_Show="False"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""SV40_PA_terminator"" -
                     translation=""MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     -
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     -
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     - GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLST \"
                     /X-Transferred_From="3.
                     pCAG-nls-Csy4-FokI-T2A-dCas9-recoded (C-terminal fusion) -
                     linker variants; 6.pCAG-nls-FokI-Csy4-T2A-Cas9-recoded
                     (N-terminal fusion) -linker variants; JDS246;
                     VC-(VC780+Csy4-Cas9)_pLKO-TRC001;
                     pCAG-Csy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK376); pCAG-Csy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64
                     recoded (BPK376frameshift);
                     pCAG-Csy4-P2A-dCas9-NLS-FLAG-VP64 recoded;
                     pCAG-Csy4-T2A-Cas9-VP64 recoded (BPK1);
                     pCAG-Csy4-T2A-Cas9-VP64 recoded (BPK2a);
                     pCAG-Csy4-T2A-Cas9-recoded (SQT834);
                     pCAG-Csy4-T2A-FokI-dCas9-recoded-nls (JAF1484);
                     pCAG-Csy4-T2A-KRAB-dCas9-recoded-nlsFLAG (NW9);
                     pCAG-Csy4-T2A-NLS-FLAG-NLS-VP64-dCas9-recoded (making
                     p125); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-KRAB recoded
                     (BPK353); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-VP64 recoded
                     (BPK326); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-VP64 recoded NO
                     CTERM FLAG;
                     pCAG-Csy4-T2A-VP64-NLS/FLAG/NLS-dCas9-recoded-nlsFLAG
                     (BPK328); pCAG-Csy4-T2A-VP64-dCas9 linker variant BbsI
                     cassette; pCAG-Csy4-T2A-VP64-dCas9-recoded-nlsFLAG
                     (BPK285); pCAG-Csy4-T2A-VP64-dCas9-recoded-nlsFLAG
                     (BPK286); pCAG-Csy4-T2A-dCas9-KRAB recoded (NW8);
                     pCAG-Csy4-T2A-dCas9-VP64 recoded (BPK3);
                     pCAG-Csy4-T2A-dCas9-VP64 recoded NO FLAG (BPK373 or 374?);
                     pCAG-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64;
                     pCAG-NLS-FLAG-NLS-dCas9-VP64 recoded;
                     pCAG-NLS-FLAG-VP64-dCas9-recoded;
                     pCAG-NLS-VP64-dCas9-recoded; pCAG-NLS-dCas9-NLS-3xFLAG
                     recoded; pCAG-NLS-dCas9-VP64 recoded; pCAG-NLS-dCas9-VP64
                     recoded CopyXXXXX;
                     pCAG-NLS-hCsy4-P2A-NLS-FLAG-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-NLS-hTtAgo (BPK633); pCAG-NLS-hTtAgo-NLS-3xFLAG
                     (BPK573); pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64;
                     pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64 D478A/D546A;
                     pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64 D478N/D546N;
                     pCAG-VP64-NLS-FLAG-NLS-dCas9-recoded-NLS-FLAG (BPK329);
                     pCAG-VP64-NLS-hTtAgo; pCAG-VP64-dCas9-recod linker variant
                     BbsI cassette; pCAG-VP64-dCas9-recoded GGGGSx5 linker;
                     pCAG-VP64-dCas9-recoded-NLS-FLAG (BPK278);
                     pCAG-VP64-hCsy4-NLS-P2A-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-dCas9-VP64;
                     pCAG-hCsy4-NLS-P2A-NLS-FLAG-NLS-hdCas9-NLS-FLAG-VP64
                     (BPK553);
                     pCAG-hCsy4-NLS-VP64-P2A-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-3xFLAG-KRAB
                     (BPK550); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64
                     delta175-307 (BPK544);
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded;
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK447);
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64-GG recoded
                     (BPK531); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-KRAB
                     (BPK454); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-VP64
                     recoded (BPK453); pCAG-hCsy4-P2A-NLS-dCas9-NLS-FLAG-VP64
                     recoded (BPK546); pCAG-hCsy4-P2A-NLS-dCas9-NLS-KRAB
                     (BPK548); pCAG-hCsy4-P2A-dCas9-NLS-FLAG-VP64 recoded
                     (BPK422); pCAG-hCsy4-P2A-dCas9-NLS-KRAB (BPK439);
                     pCAG-hCsy4-P2A-dCas9-NLS-VP64 recoded (BPK450);
                     pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK428); pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-KRAB
                     (BPK444); pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-VP64
                     recoded (BPK437); pCAG-hCsy4-T2A-dCas9-NLS-FLAG-VP64
                     recoded (BPK426); pCAG-hCsy4-T2A-dCas9-NLS-KRAB (BPK442);
                     pCAG-hCsy4-T2A-dCas9-NLS-VP64 recoded (BPK435);
                     pCAG-hCsy4-T2A-nls-hFokI-dhCas9-nls-3XFLAG (SQT1601);
                     pCAG-hTtAgo; pCAG-hTtAgo-NLS; pCAG-hTtAgo-NLS-3xFLAG;
                     pCAG-nls-FokI-Csy4-T2A-dCas9-recoded (N-terminal fusion
                     CK1891); pCMV-NLS-hTtAgo-NLS-3xFLAG; pCW-Cas9 (modified);
                     pCW-Cas9 Activator; pCW-Cas9 N-F-N Activator; pCW-Cas9
                     N-F-N Activator-GG; pCW-EFS-Puro-P2A-NFN-dCas9-VP64;
                     pCW-NLS-dCas9-VP64 EFS-Puro-T2A-rTta3-WPRE;
                     pEF1a-Csy4-T2A-NLS-FLAG-NLS-dCas9-VP64 recoded;
                     pEF1a-Csy4-T2A-dCas9-VP64 recoded;
                     pEF1a-Csy4-T2A-dCas9-VP64 recoded TRUNC;
                     pEF1a-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded;
                     pEFS-Csy4-T2A-dCas9-VP64 recoded;
                     pEFS-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK533); pLV-CMVEI.EGFPDS-iNpLTRSEBBA6_0693;
                     pLenti-TREminCMV-NLS-3xFLAG-NLS-dCas9-VP64
                     EFS-Puro-T2A-rTta3-WPRE; pLenti-TREminCMV-NLS-dCas9-VP64
                     EFS-Puro-T2A-rTta3-WPRE; pMLM3705; pMLM3705 annotation
                     mess; pWPXLd (modified); pcDNA5/FRT;
                     pcDNA5/FRT-tdtomato-AAVS1 site 1;
                     pcDNA5/FRT-tdtomato-AAVS1 site 1 in wrong way;
                     pcDNA5/FRT-tdtomato-AAVS1 site 2"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pCMV-T7-Cas9 (MLM3613), pCMV-T7-Cas9
                     delta NcoI"
                     /Transferred_Similarity="100.00%"
                     /modified_by="KleinstiverLab8"
                     /label="SV40_PA_terminator"
ORIGIN      
        1 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
       61 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
      121 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      181 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      241 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
      301 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
      361 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
      421 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
      481 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
      541 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
      601 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
      661 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
      721 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
      781 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
      841 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
      901 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
      961 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     1021 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     1081 taccgcgcga accacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     1141 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     1201 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     1261 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     1321 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     1381 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     1441 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     1501 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     1561 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     1621 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     1681 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     1741 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     1801 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     1861 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     1921 cccgaaaagt gccacctgac gtcgacggat cgggagatcg atctcccgat cccctagggt
     1981 cgactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     2041 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     2101 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
     2161 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
     2221 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     2281 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     2341 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     2401 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     2461 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     2521 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     2581 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     2641 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     2701 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     2761 gagctggttt agtgaaccgt cagatccgct agagatccgc ggccgctaat acgactcact
     2821 atagggagag ccgccaccat gagcccatct aagaaaaacg gcagaagcgg acctcaacct
     2881 cacaagcggt gggtctttac cctgaacaac cccagcgaag atgagagaaa gaagatccgg
     2941 gacctgccta tcagcctgtt cgactacttc atcgtgggcg aggaaggaaa tgaggaaggc
     3001 cggacccccc acctgcaggg cttcgccaac ttcgtgaaaa agcagacctt caacaaggtg
     3061 aagtggtacc tgggcgctag atgccacatc gagaaggcca agggcaccga ccagcagaac
     3121 aaagagtact gcagcaagga aggcaatctg ctgatggaat gtggcgcccc tagatcccag
     3181 ggacagagaa gcggaggctc tagcggctcc gagacacccg gcacatctga gtccgccacc
     3241 cctgagagca gcggcggcag cgacaagaag tacagcatcg gcctggacat cggcaccaac
     3301 tctgtgggct gggccgtgat caccgacgag tacaaggtgc ccagcaagaa attcaaggtg
     3361 ctgggcaaca ccgaccggca cagcatcaag aagaacctga tcggagccct gctgttcgac
     3421 agcggcgaaa cagccgaggc cacccggctg aagagaaccg ccagaagaag atacaccaga
     3481 cggaagaacc ggatctgcta tctgcaagag atcttcagca acgagatggc caaggtggac
     3541 gacagcttct tccacagact ggaagagtcc ttcctggtgg aagaggataa gaagcacgag
     3601 cggcacccca tcttcggcaa catcgtggac gaggtggcct accacgagaa gtaccccacc
     3661 atctaccacc tgagaaagaa actggtggac agcaccgaca aggccgacct gcggctgatc
     3721 tatctggccc tggcccacat gatcaagttc cggggccact tcctgatcga gggcgacctg
     3781 aaccccgaca acagcgacgt ggacaagctg ttcatccagc tggtgcagac ctacaaccag
     3841 ctgttcgagg aaaaccccat caacgccagc ggcgtggacg ccaaggccat cctgtctgcc
     3901 agactgagca agagcagacg gctggaaaat ctgatcgccc agctgcccgg cgagaagaag
     3961 aatggcctgt tcggaaacct gattgccctg agcctgggcc tgacccccaa cttcaagagc
     4021 aacttcgacc tggccgagga tgccaaactg cagctgagca aggacaccta cgacgacgac
     4081 ctggacaacc tgctggccca gatcggcgac cagtacgccg acctgtttct ggccgccaag
     4141 aacctgtccg acgccatcct gctgagcgac atcctgagag tgaacaccga gatcaccaag
     4201 gcccccctga gcgcctctat gatcaagaga tacgacgagc accaccagga cctgaccctg
     4261 ctgaaagctc tcgtgcggca gcagctgcct gagaagtaca aagagatttt cttcgaccag
     4321 agcaagaacg gctacgccgg ctacattgac ggcggagcca gccaggaaga gttctacaag
     4381 ttcatcaagc ccatcctgga aaagatggac ggcaccgagg aactgctcgt gaagctgaac
     4441 agagaggacc tgctgcggaa gcagcggacc ttcgacaacg gcagcatccc ccaccagatc
     4501 cacctgggag agctgcacgc cattctgcgg cggcaggaag atttttaccc attcctgaag
     4561 gacaaccggg aaaagatcga gaagatcctg accttccgca tcccctacta cgtgggccct
     4621 ctggccaggg gaaacagcag attcgcctgg atgaccagaa agagcgagga aaccatcacc
     4681 ccctggaact tcgaggaagt ggtggacaag ggcgcttccg cccagagctt catcgagcgg
     4741 atgaccaact tcgataagaa cctgcccaac gagaaggtgc tgcccaagca cagcctgctg
     4801 tacgagtact tcaccgtgta taacgagctg accaaagtga aatacgtgac cgagggaatg
     4861 agaaagcccg ccttcctgag cggcgagcag aaaaaggcca tcgtggacct gctgttcaag
     4921 accaaccgga aagtgaccgt gaagcagctg aaagaggact acttcaagaa aatcgagtgc
     4981 ttcgactccg tggaaatctc cggcgtggaa gatcggttca acgcctccct gggcacatac
     5041 cacgatctgc tgaaaattat caaggacaag gacttcctgg acaatgagga aaacgaggac
     5101 attctggaag atatcgtgct gaccctgaca ctgtttgagg acagagagat gatcgaggaa
     5161 cggctgaaaa cctatgccca cctgttcgac gacaaagtga tgaagcagct gaagcggcgg
     5221 agatacaccg gctggggcag gctgagccgg aagctgatca acggcatccg ggacaagcag
     5281 tccggcaaga caatcctgga tttcctgaag tccgacggct tcgccaacag aaacttcatg
     5341 cagctgatcc acgacgacag cctgaccttt aaagaggaca tccagaaagc ccaggtgtcc
     5401 ggccagggcg atagcctgca cgagcacatt gccaatctgg ccggcagccc cgccattaag
     5461 aagggcatcc tgcagacagt gaaggtggtg gacgagctcg tgaaagtgat gggccggcac
     5521 aagcccgaga acatcgtgat cgaaatggcc agagagaacc agaccaccca gaagggacag
     5581 aagaacagcc gcgagagaat gaagcggatc gaagagggca tcaaagagct gggcagccag
     5641 atcctgaaag aacaccccgt ggaaaacacc cagctgcaga acgagaagct gtacctgtac
     5701 tacctgcaga atgggcggga tatgtacgtg gaccaggaac tggacatcaa ccggctgtcc
     5761 gactacgatg tggacgctat cgtgcctcag agctttctga aggacgactc catcgacaac
     5821 aaggtgctga ccagaagcga caagaaccgg ggcaagagcg acaacgtgcc ctccgaagag
     5881 gtcgtgaaga agatgaagaa ctactggcgg cagctgctga acgccaagct gattacccag
     5941 agaaagttcg acaatctgac caaggccgag agaggcggcc tgagcgaact ggataaggcc
     6001 ggcttcatca agagacagct ggtggaaacc cggcagatca caaagcacgt ggcacagatc
     6061 ctggactccc ggatgaacac taagtacgac gagaatgaca agctgatccg ggaagtgaaa
     6121 gtgatcaccc tgaagtccaa gctggtgtcc gatttccgga aggatttcca gttttacaaa
     6181 gtgcgcgaga tcaacaacta ccaccacgcc cacgacgcct acctgaacgc cgtcgtggga
     6241 accgccctga tcaaaaagta ccctaagctg gaaagcgagt tcgtgtacgg cgactacaag
     6301 gtgtacgacg tgcggaagat gatcgccaag agcgagcagg aaatcggcaa ggctaccgcc
     6361 aagtacttct tctacagcaa catcatgaac tttttcaaga ccgagattac cctggccaac
     6421 ggcgagatcc ggaagcggcc tctgatcgag acaaacggcg aaaccgggga gatcgtgtgg
     6481 gataagggcc gggattttgc caccgtgcgg aaagtgctga gcatgcccca agtgaatatc
     6541 gtgaaaaaga ccgaggtgca gacaggcggc ttcagcaaag agtctatcct gcccaagagg
     6601 aacagcgata agctgatcgc cagaaagaag gactgggacc ctaagaagta cggcggcttc
     6661 gacagcccca ccgtggccta ttctgtgctg gtggtggcca aagtggaaaa gggcaagtcc
     6721 aagaaactga agagtgtgaa agagctgctg gggatcacca tcatggaaag aagcagcttc
     6781 gagaagaatc ccatcgactt tctggaagcc aagggctaca aagaagtgaa aaaggacctg
     6841 atcatcaagc tgcctaagta ctccctgttc gagctggaaa acggccggaa gagaatgctg
     6901 gcctctgccg gcgaactgca gaagggaaac gaactggccc tgccctccaa atatgtgaac
     6961 ttcctgtacc tggccagcca ctatgagaag ctgaagggct cccccgagga taatgagcag
     7021 aaacagctgt ttgtggaaca gcacaagcac tacctggacg agatcatcga gcagatcagc
     7081 gagttctcca agagagtgat cctggccgac gctaatctgg acaaagtgct gtccgcctac
     7141 aacaagcacc gggataagcc catcagagag caggccgaga atatcatcca cctgtttacc
     7201 ctgaccaatc tgggagcccc tgccgccttc aagtactttg acaccaccat cgaccggaag
     7261 aggtacacca gcaccaaaga ggtgctggac gccaccctga tccaccagag catcaccggc
     7321 ctgtacgaga cacggatcga cctgtctcag ctgggaggtg actccggcgg aagctctggt
     7381 ggcagcaagc ggaccgccga cggctctgaa ttcgagagcc ctaagaagaa aagaaaggtg
     7441 agcggaggct ctagcggcgg aagcgttatc tcctacgaca actacgtgac catcctggac
     7501 gaagagacac tgaaggcttg gatcgctaaa ctggaaaaag cccctgtgtt cgccttcgcc
     7561 accgccaccg actccttaga taacatcagc gccaacctgg tgggcctgtc tttcgccatc
     7621 gagcctggcg tggccgccta catccccgtg gcccacgatt acctggatgc cccagatcaa
     7681 atcagcagag agagagcact ggagctcctg aagcccctgc tggaggacga gaaggccctg
     7741 aaggtgggac agaacctgaa atacgatagg ggaatcctgg ctaattacgg catcgagctg
     7801 agaggcatcg ccttcgatac catgctggaa tcttacatcc tgaacagcgt ggccggccgc
     7861 cacgacatgg acagcctggc tgaacggtgg ctgaagcaca aaaccatcac cttcgaagag
     7921 atcgccggaa agggcaagaa ccagctgaca tttaatcaga tcgcactgga agaagccggc
     7981 agatacgccg ctgaggacgc cgacgtgacc cttcagctgc acctgaagat gtggcccgat
     8041 ctgcagaagc acaagggccc actaaacgtg ttcgagaaca tcgagatgcc tctggtgccc
     8101 gtcctgagca gaatcgagag gaatggcgtg aagatcgacc ccaaggtgct gcacaaccac
     8161 agcgaggaac tgacactgcg gctggccgag ctggaaaaga aggcccacga aatcgccggc
     8221 gaggagttca acctgtctag caccaagcag ctacaaacaa tcctgttcga gaagcagggc
     8281 attaagcctc tgaaaaagac acctggcgga gcccccagca ccagcgaaga agtgctggag
     8341 gaactggcgc tggattaccc tctgcccaaa gtgatcctgg aatatagagg actggccaag
     8401 ctgaagtcta catacaccga caagctgcct ctgatgatca accccaagac cggccgggtg
     8461 catacatctt atcaccaggc cgtgacagcc accggccggc tgagcagcac agatcctaac
     8521 ctgcaaaaca tcccagtgcg gaacgaagag ggcagaagaa tcagacaggc tttcattgcc
     8581 cctgaggact acgtcatcgt gtctgctgcg tacagccaga tagaactgag aatcatggcc
     8641 catctgtctc gggacaaggg cctgctgacc gcttttgcag aaggcaagga catccacaga
     8701 gccaccgctg ccgaagtgtt cggcctgcct ctggaaacag tgaccagcga gcaaagaagg
     8761 tccgccaagg ccattaactt cggcctgatc tacggcatga gcgcctttgg cctggccaga
     8821 cagctcaaca tccctagaaa ggaggcccag aaatacatgg acctgtactt cgagcggtac
     8881 cccggcgtgc tggagtacat ggaacggacc agagcccagg ctaaagagca gggatacgtg
     8941 gaaaccctgg atggccggag actgtatctg cctgacatca agagcagcaa cggcgccaga
     9001 agagccgccg ctgagagagc tgctatcaac gcccctatgc agggcaccgc cgccgacatc
     9061 atcaagcggg ccatgatcgc cgtggacgcc tggctgcagg ccgagcagcc tagagtgcgg
     9121 atgatcatgc aggtgcacgc ggagctggtg tttgaggtgc acaaggacga cgtggatgct
     9181 gtggccaagc agattcacca gctgatggaa aattgcacca gactggacgt gccactgctg
     9241 gtcgaggttg gaagcggaga gaattgggac caggcccact ctggcggctc aaaaagaacc
     9301 gccgacggca gcgaattcga gtctcccaag aagaagagga aagtctgacc ggtcatcatc
     9361 accatcacca ttgagtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     9421 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     9481 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     9541 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     9601 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctcgatac
     9661 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     9721 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctagg
     9781 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     9841 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     9901 tgcgtattg
//
