LOCUS       pCMV-T7-RepBM-nCas9-EcKlenow_(JO606)        9948 bp    DNA     circular UNK 24-NOV-2023
DEFINITION  .
ACCESSION   <unknown
KEYWORDS    .
SOURCE      .
  ORGANISM  .
FEATURES             Location/Qualifiers
     CDS             complement(171..1031)
                     /gene="Ampicillin"
                     /Color="#777777"
                     /modified_by="ben"
                     /Original_Translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVL
                     GNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKV
                     DDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADL
                     RLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAK
                     AILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLS
                     KDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRY
                     DEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKM
                     DGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIE
                     KILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFD
                     KNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNR
                     KVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDI
                     LEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDK
                     QSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSP
                     AIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIK
                     ELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFL
                     KDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAER
                     GGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLV
                     SDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKM
                     IAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRD
                     FATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSP
                     TVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLI
                     IKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNE
                     QKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIH
                     LFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDG
                     SPKKKRKVSSDYKDHDGDYKDHDIDYKDDDDKAAG*"
                     /label="Ampicillin"
     promoter        complement(1032..1136)
                     /X-Transferred_From="JDS246; pCMV-NLS-hTtAgo-NLS-3xFLAG;
                     pLV-CMVEI.EGFPDS-iNpLTRSEBBA6_0693; pLVX-TetOne-puro;
                     pMLM3705; pMLM3705 annotation mess"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pCMV-T7-Cas9 (MLM3613), pCMV-T7-Cas9
                     delta NcoI"
                     /Transferred_Similarity="100.00%"
                     /label="AmpR promoter"
     promoter        1420..1785
                     /SerialCloner_Color="&h03C000"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /X-Transferred_From="3.
                     pCAG-nls-Csy4-FokI-T2A-dCas9-recoded (C-terminal fusion) -
                     linker variants; 6.pCAG-nls-FokI-Csy4-T2A-Cas9-recoded
                     (N-terminal fusion) -linker variants; JDS246;
                     pCAG-Csy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK376); pCAG-Csy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64
                     recoded (BPK376frameshift);
                     pCAG-Csy4-P2A-dCas9-NLS-FLAG-VP64 recoded;
                     pCAG-Csy4-T2A-Cas9-VP64 recoded (BPK1);
                     pCAG-Csy4-T2A-Cas9-VP64 recoded (BPK2a);
                     pCAG-Csy4-T2A-Cas9-recoded (SQT834);
                     pCAG-Csy4-T2A-FokI-dCas9-recoded-nls (JAF1484);
                     pCAG-Csy4-T2A-KRAB-dCas9-recoded-nlsFLAG (NW9);
                     pCAG-Csy4-T2A-NLS-FLAG-NLS-VP64-dCas9-recoded (making
                     p125); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-KRAB recoded
                     (BPK353); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-VP64 recoded
                     (BPK326); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-VP64 recoded NO
                     CTERM FLAG;
                     pCAG-Csy4-T2A-VP64-NLS/FLAG/NLS-dCas9-recoded-nlsFLAG
                     (BPK328); pCAG-Csy4-T2A-VP64-dCas9 linker variant BbsI
                     cassette; pCAG-Csy4-T2A-VP64-dCas9-recoded-nlsFLAG
                     (BPK285); pCAG-Csy4-T2A-VP64-dCas9-recoded-nlsFLAG
                     (BPK286); pCAG-Csy4-T2A-dCas9-KRAB recoded (NW8);
                     pCAG-Csy4-T2A-dCas9-VP64 recoded (BPK3);
                     pCAG-Csy4-T2A-dCas9-VP64 recoded NO FLAG (BPK373 or 374?);
                     pCAG-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64;
                     pCAG-NLS-FLAG-NLS-dCas9-VP64 recoded;
                     pCAG-NLS-FLAG-VP64-dCas9-recoded;
                     pCAG-NLS-VP64-dCas9-recoded; pCAG-NLS-dCas9-NLS-3xFLAG
                     recoded; pCAG-NLS-dCas9-VP64 recoded; pCAG-NLS-dCas9-VP64
                     recoded CopyXXXXX;
                     pCAG-NLS-hCsy4-P2A-NLS-FLAG-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-NLS-hTtAgo (BPK633); pCAG-NLS-hTtAgo-NLS-3xFLAG
                     (BPK573); pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64;
                     pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64 D478A/D546A;
                     pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64 D478N/D546N;
                     pCAG-VP64-NLS-FLAG-NLS-dCas9-recoded-NLS-FLAG (BPK329);
                     pCAG-VP64-NLS-hTtAgo; pCAG-VP64-dCas9-recod linker variant
                     BbsI cassette; pCAG-VP64-dCas9-recoded GGGGSx5 linker;
                     pCAG-VP64-dCas9-recoded-NLS-FLAG (BPK278);
                     pCAG-VP64-hCsy4-NLS-P2A-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-dCas9-VP64;
                     pCAG-hCsy4-NLS-P2A-NLS-FLAG-NLS-hdCas9-NLS-FLAG-VP64
                     (BPK553);
                     pCAG-hCsy4-NLS-VP64-P2A-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-3xFLAG-KRAB
                     (BPK550); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64
                     delta175-307 (BPK544);
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded;
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK447);
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64-GG recoded
                     (BPK531); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-KRAB
                     (BPK454); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-VP64
                     recoded (BPK453); pCAG-hCsy4-P2A-NLS-dCas9-NLS-FLAG-VP64
                     recoded (BPK546); pCAG-hCsy4-P2A-NLS-dCas9-NLS-KRAB
                     (BPK548); pCAG-hCsy4-P2A-dCas9-NLS-FLAG-VP64 recoded
                     (BPK422); pCAG-hCsy4-P2A-dCas9-NLS-KRAB (BPK439);
                     pCAG-hCsy4-P2A-dCas9-NLS-VP64 recoded (BPK450);
                     pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK428); pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-KRAB
                     (BPK444); pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-VP64
                     recoded (BPK437); pCAG-hCsy4-T2A-dCas9-NLS-FLAG-VP64
                     recoded (BPK426); pCAG-hCsy4-T2A-dCas9-NLS-KRAB (BPK442);
                     pCAG-hCsy4-T2A-dCas9-NLS-VP64 recoded (BPK435);
                     pCAG-hCsy4-T2A-nls-hFokI-dhCas9-nls-3XFLAG (SQT1601);
                     pCAG-hTtAgo; pCAG-hTtAgo-NLS; pCAG-hTtAgo-NLS-3xFLAG;
                     pCAG-nls-FokI-Csy4-T2A-dCas9-recoded (N-terminal fusion
                     CK1891); pCMV-NLS-hTtAgo-NLS-3xFLAG; pLenti-CAG-Puro-DEST;
                     pMLM3705; pMLM3705 annotation mess"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pCMV-T7-Cas9 (MLM3613), pCMV-T7-Cas9
                     delta NcoI"
                     /Transferred_Similarity="100.00%"
                     /modified_by="lha16"
                     /label="CMV Enhancer"
     promoter        complement(1469..1497)
                     /SerialCloner_Color="&hF08080"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""AmpR_promoter"" -
                     translation=""MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     -
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     -
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     - DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWM \"
                     /X-Transferred_From="3.
                     pCAG-nls-Csy4-FokI-T2A-dCas9-recoded (C-terminal fusion) -
                     linker variants; 6.pCAG-nls-FokI-Csy4-T2A-Cas9-recoded
                     (N-terminal fusion) -linker variants; JDS246;
                     pCAG-Csy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK376); pCAG-Csy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64
                     recoded (BPK376frameshift);
                     pCAG-Csy4-P2A-dCas9-NLS-FLAG-VP64 recoded;
                     pCAG-Csy4-T2A-Cas9-VP64 recoded (BPK1);
                     pCAG-Csy4-T2A-Cas9-VP64 recoded (BPK2a);
                     pCAG-Csy4-T2A-Cas9-recoded (SQT834);
                     pCAG-Csy4-T2A-FokI-dCas9-recoded-nls (JAF1484);
                     pCAG-Csy4-T2A-KRAB-dCas9-recoded-nlsFLAG (NW9);
                     pCAG-Csy4-T2A-NLS-FLAG-NLS-VP64-dCas9-recoded (making
                     p125); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-KRAB recoded
                     (BPK353); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-VP64 recoded
                     (BPK326); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-VP64 recoded NO
                     CTERM FLAG;
                     pCAG-Csy4-T2A-VP64-NLS/FLAG/NLS-dCas9-recoded-nlsFLAG
                     (BPK328); pCAG-Csy4-T2A-VP64-dCas9 linker variant BbsI
                     cassette; pCAG-Csy4-T2A-VP64-dCas9-recoded-nlsFLAG
                     (BPK285); pCAG-Csy4-T2A-VP64-dCas9-recoded-nlsFLAG
                     (BPK286); pCAG-Csy4-T2A-dCas9-KRAB recoded (NW8);
                     pCAG-Csy4-T2A-dCas9-VP64 recoded (BPK3);
                     pCAG-Csy4-T2A-dCas9-VP64 recoded NO FLAG (BPK373 or 374?);
                     pCAG-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64;
                     pCAG-NLS-FLAG-NLS-dCas9-VP64 recoded;
                     pCAG-NLS-FLAG-VP64-dCas9-recoded;
                     pCAG-NLS-VP64-dCas9-recoded; pCAG-NLS-dCas9-NLS-3xFLAG
                     recoded; pCAG-NLS-dCas9-VP64 recoded; pCAG-NLS-dCas9-VP64
                     recoded CopyXXXXX;
                     pCAG-NLS-hCsy4-P2A-NLS-FLAG-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-NLS-hTtAgo (BPK633); pCAG-NLS-hTtAgo-NLS-3xFLAG
                     (BPK573); pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64;
                     pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64 D478A/D546A;
                     pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64 D478N/D546N;
                     pCAG-VP64-NLS-FLAG-NLS-dCas9-recoded-NLS-FLAG (BPK329);
                     pCAG-VP64-NLS-hTtAgo; pCAG-VP64-dCas9-recod linker variant
                     BbsI cassette; pCAG-VP64-dCas9-recoded GGGGSx5 linker;
                     pCAG-VP64-dCas9-recoded-NLS-FLAG (BPK278);
                     pCAG-VP64-hCsy4-NLS-P2A-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-dCas9-VP64;
                     pCAG-hCsy4-NLS-P2A-NLS-FLAG-NLS-hdCas9-NLS-FLAG-VP64
                     (BPK553);
                     pCAG-hCsy4-NLS-VP64-P2A-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-3xFLAG-KRAB
                     (BPK550); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64
                     delta175-307 (BPK544);
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded;
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK447);
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64-GG recoded
                     (BPK531); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-KRAB
                     (BPK454); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-VP64
                     recoded (BPK453); pCAG-hCsy4-P2A-NLS-dCas9-NLS-FLAG-VP64
                     recoded (BPK546); pCAG-hCsy4-P2A-NLS-dCas9-NLS-KRAB
                     (BPK548); pCAG-hCsy4-P2A-dCas9-NLS-FLAG-VP64 recoded
                     (BPK422); pCAG-hCsy4-P2A-dCas9-NLS-KRAB (BPK439);
                     pCAG-hCsy4-P2A-dCas9-NLS-VP64 recoded (BPK450);
                     pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK428); pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-KRAB
                     (BPK444); pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-VP64
                     recoded (BPK437); pCAG-hCsy4-T2A-dCas9-NLS-FLAG-VP64
                     recoded (BPK426); pCAG-hCsy4-T2A-dCas9-NLS-KRAB (BPK442);
                     pCAG-hCsy4-T2A-dCas9-NLS-VP64 recoded (BPK435);
                     pCAG-hCsy4-T2A-nls-hFokI-dhCas9-nls-3XFLAG (SQT1601);
                     pCAG-hTtAgo; pCAG-hTtAgo-NLS; pCAG-hTtAgo-NLS-3xFLAG;
                     pCAG-nls-FokI-Csy4-T2A-dCas9-recoded (N-terminal fusion
                     CK1891); pCMV-NLS-hTtAgo-NLS-3xFLAG; pLenti-CAG-Puro-DEST;
                     pMLM3705; pMLM3705 annotation mess; pcDNA5/FRT"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pCMV-T7-Cas9 (MLM3613), pCMV-T7-Cas9
                     delta NcoI"
                     /Transferred_Similarity="100.00%"
                     /label="AmpR_promoter"
     promoter        1486..1773
                     /SerialCloner_Color="&hDC143C"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""CAG_enhancer"" - 84..371 \"
                     /X-Transferred_From="3.
                     pCAG-nls-Csy4-FokI-T2A-dCas9-recoded (C-terminal fusion) -
                     linker variants; 6.pCAG-nls-FokI-Csy4-T2A-Cas9-recoded
                     (N-terminal fusion) -linker variants; JDS246;
                     pCAG-AttB-Csy4-VEGFA1-3 (BPK318); pCAG-CFP;
                     pCAG-Csy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK376); pCAG-Csy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64
                     recoded (BPK376frameshift);
                     pCAG-Csy4-P2A-dCas9-NLS-FLAG-VP64 recoded;
                     pCAG-Csy4-T2A-Cas9-VP64 recoded (BPK1);
                     pCAG-Csy4-T2A-Cas9-VP64 recoded (BPK2a);
                     pCAG-Csy4-T2A-Cas9-recoded (SQT834);
                     pCAG-Csy4-T2A-FokI-dCas9-recoded-nls (JAF1484);
                     pCAG-Csy4-T2A-KRAB-dCas9-recoded-nlsFLAG (NW9);
                     pCAG-Csy4-T2A-NLS-FLAG-NLS-VP64-dCas9-recoded (making
                     p125); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-KRAB recoded
                     (BPK353); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-VP64 recoded
                     (BPK326); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-VP64 recoded NO
                     CTERM FLAG;
                     pCAG-Csy4-T2A-VP64-NLS/FLAG/NLS-dCas9-recoded-nlsFLAG
                     (BPK328); pCAG-Csy4-T2A-VP64-dCas9 linker variant BbsI
                     cassette; pCAG-Csy4-T2A-VP64-dCas9-recoded-nlsFLAG
                     (BPK285); pCAG-Csy4-T2A-VP64-dCas9-recoded-nlsFLAG
                     (BPK286); pCAG-Csy4-T2A-dCas9-KRAB recoded (NW8);
                     pCAG-Csy4-T2A-dCas9-VP64 recoded (BPK3);
                     pCAG-Csy4-T2A-dCas9-VP64 recoded NO FLAG (BPK373 or 374?);
                     pCAG-Csy4site2-gRNA-Csy4site2 (SQT1468) + 1 site;
                     pCAG-Csy4site2-gRNA-Csy4site2 (SQT1468) + 4 sites;
                     pCAG-Csy4site2-gRNA-Csy4site2 BsaI;
                     pCAG-Csy4site2-gRNA-Csy4site2 BsaI cassette; pCAG-DEST
                     (BPK88); pCAG-DEST V2;
                     pCAG-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64;
                     pCAG-NLS-FLAG-NLS-dCas9-VP64 recoded;
                     pCAG-NLS-FLAG-VP64-dCas9-recoded;
                     pCAG-NLS-VP64-dCas9-recoded; pCAG-NLS-dCas9-NLS-3xFLAG
                     recoded; pCAG-NLS-dCas9-VP64 recoded; pCAG-NLS-dCas9-VP64
                     recoded CopyXXXXX;
                     pCAG-NLS-hCsy4-P2A-NLS-FLAG-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-NLS-hTtAgo (BPK633); pCAG-NLS-hTtAgo-NLS-3xFLAG
                     (BPK573); pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64;
                     pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64 D478A/D546A;
                     pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64 D478N/D546N;
                     pCAG-VP64-NLS-FLAG-NLS-dCas9-recoded-NLS-FLAG (BPK329);
                     pCAG-VP64-NLS-hTtAgo; pCAG-VP64-dCas9-recod linker variant
                     BbsI cassette; pCAG-VP64-dCas9-recoded GGGGSx5 linker;
                     pCAG-VP64-dCas9-recoded-NLS-FLAG (BPK278);
                     pCAG-VP64-hCsy4-NLS-P2A-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-dCas9-VP64;
                     pCAG-hCsy4-NLS-P2A-NLS-FLAG-NLS-hdCas9-NLS-FLAG-VP64
                     (BPK553);
                     pCAG-hCsy4-NLS-VP64-P2A-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-3xFLAG-KRAB
                     (BPK550); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64
                     delta175-307 (BPK544);
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded;
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK447);
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64-GG recoded
                     (BPK531); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-KRAB
                     (BPK454); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-VP64
                     recoded (BPK453); pCAG-hCsy4-P2A-NLS-dCas9-NLS-FLAG-VP64
                     recoded (BPK546); pCAG-hCsy4-P2A-NLS-dCas9-NLS-KRAB
                     (BPK548); pCAG-hCsy4-P2A-dCas9-NLS-FLAG-VP64 recoded
                     (BPK422); pCAG-hCsy4-P2A-dCas9-NLS-KRAB (BPK439);
                     pCAG-hCsy4-P2A-dCas9-NLS-VP64 recoded (BPK450);
                     pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK428); pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-KRAB
                     (BPK444); pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-VP64
                     recoded (BPK437); pCAG-hCsy4-T2A-dCas9-NLS-FLAG-VP64
                     recoded (BPK426); pCAG-hCsy4-T2A-dCas9-NLS-KRAB (BPK442);
                     pCAG-hCsy4-T2A-dCas9-NLS-VP64 recoded (BPK435);
                     pCAG-hCsy4-T2A-nls-hFokI-dhCas9-nls-3XFLAG (SQT1601);
                     pCAG-hTtAgo; pCAG-hTtAgo-NLS; pCAG-hTtAgo-NLS-3xFLAG;
                     pCAG-nls-FokI-Csy4-T2A-dCas9-recoded (N-terminal fusion
                     CK1891); pCMV-NLS-hTtAgo-NLS-3xFLAG; pLenti-CAG-Puro-DEST;
                     pMLM3705; pMLM3705 annotation mess"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pCMV-T7-Cas9 (MLM3613), pCMV-T7-Cas9
                     delta NcoI"
                     /Transferred_Similarity="100.00%"
                     /modified_by="ben"
                     /label="CAG_enhancer"
     promoter        1786..2010
                     /label="CMV_promoter"
     promoter        2031..2050
                     /X-Transferred_From="pCRII-TOPO"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pCMV-T7-Cas9 (MLM3613), pCMV-T7-Cas9
                     delta NcoI"
                     /Transferred_Similarity="100.00%"
                     /modified_by="lha16"
                     /label="T7 promoter"
     motif           2054..2066
                     /created_by="ben"
                     /label="Kozak"
     CDS             2063..2452
                     /created_by="joana"
                     /Color="#ffbb33"
                     /modified_by="lha16"
                     /label="RepBM HUHe"
     CDS             2453..2524
                     /Color="#ff7400"
                     /created_by="lha16"
                     /label="GS-XTEN-GS linker"
     CDS             2522..6625
                     /product="nickase mutant of the Cas9 endonuclease from
                     theStreptococcus pyogenes Type II CRISPR/Cas system"
                     /note="generates RNA-guided single strand nicks in DNA
                     dueto the H840A mutation in the HNH catalytic domain"
                     /Original_Translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLG
                     NTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVD
                     DSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
                     LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKA
                     ILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSK
                     DTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYD
                     EHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMD
                     GTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
                     ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDK
                     NLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRK
                     VTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDIL
                     EDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQ
                     SGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPA
                     IKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKE
                     LGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLK
                     DDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERG
                     GLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVS
                     DFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMI
                     AKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDF
                     ATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPT
                     VAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLII
                     KLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQ
                     KQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHL
                     FTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
                     /modified_by="chenp"
                     /modified_by="kleinstivermac7"
                     /Color="#eeeeee"
                     /modified_by="ben"
                     /label="nSpCas9(H840A)"
     CDS             5039..5041
                     /created_by="ben"
                     /label="H840A"
     CDS             6626..6649
                     /Color="#ff7400"
                     /created_by="ben"
                     /label="GS linker"
     CDS             6650..6703
                     /created_by="chenp"
                     /Color="#6bba70"
                     /modified_by="ben"
                     /label="BPNLS"
     CDS             6704..6727
                     /Color="#ff7400"
                     /created_by="ben"
                     /label="GS linker"
     CDS             6728..8542
                     /created_by="KleinstiverLab8"
                     /modified_by="KleinstiverLab8"
                     /Color="#ee0077"
                     /modified_by="ben"
                     /label="EcKlenow(-exo;D355A/D357A)"
     CDS             8543..8554
                     /Color="#ff7400"
                     /created_by="ben"
                     /label="GS linker"
     CDS             8555..8608
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /Original_Translation="KRTADGSEFEPKKKRKV"
                     /Color="#6bba70"
                     /modified_by="ben"
                     /label="BPNLS"
     CDS             8609..8611
                     /Color="#000000"
                     /created_by="ben"
                     /label
     terminator      8660..8887
                     /label="bGH_PA_terminator"
     terminator      9146..9277
                     /SerialCloner_Color="&hF08080"
                     /SerialCloner_Show="False"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""SV40_PA_terminator"" -
                     translation=""MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     -
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     -
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     - GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLST \"
                     /X-Transferred_From="3.
                     pCAG-nls-Csy4-FokI-T2A-dCas9-recoded (C-terminal fusion) -
                     linker variants; 6.pCAG-nls-FokI-Csy4-T2A-Cas9-recoded
                     (N-terminal fusion) -linker variants; JDS246;
                     VC-(VC780+Csy4-Cas9)_pLKO-TRC001;
                     pCAG-Csy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK376); pCAG-Csy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64
                     recoded (BPK376frameshift);
                     pCAG-Csy4-P2A-dCas9-NLS-FLAG-VP64 recoded;
                     pCAG-Csy4-T2A-Cas9-VP64 recoded (BPK1);
                     pCAG-Csy4-T2A-Cas9-VP64 recoded (BPK2a);
                     pCAG-Csy4-T2A-Cas9-recoded (SQT834);
                     pCAG-Csy4-T2A-FokI-dCas9-recoded-nls (JAF1484);
                     pCAG-Csy4-T2A-KRAB-dCas9-recoded-nlsFLAG (NW9);
                     pCAG-Csy4-T2A-NLS-FLAG-NLS-VP64-dCas9-recoded (making
                     p125); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-KRAB recoded
                     (BPK353); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-VP64 recoded
                     (BPK326); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-VP64 recoded NO
                     CTERM FLAG;
                     pCAG-Csy4-T2A-VP64-NLS/FLAG/NLS-dCas9-recoded-nlsFLAG
                     (BPK328); pCAG-Csy4-T2A-VP64-dCas9 linker variant BbsI
                     cassette; pCAG-Csy4-T2A-VP64-dCas9-recoded-nlsFLAG
                     (BPK285); pCAG-Csy4-T2A-VP64-dCas9-recoded-nlsFLAG
                     (BPK286); pCAG-Csy4-T2A-dCas9-KRAB recoded (NW8);
                     pCAG-Csy4-T2A-dCas9-VP64 recoded (BPK3);
                     pCAG-Csy4-T2A-dCas9-VP64 recoded NO FLAG (BPK373 or 374?);
                     pCAG-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64;
                     pCAG-NLS-FLAG-NLS-dCas9-VP64 recoded;
                     pCAG-NLS-FLAG-VP64-dCas9-recoded;
                     pCAG-NLS-VP64-dCas9-recoded; pCAG-NLS-dCas9-NLS-3xFLAG
                     recoded; pCAG-NLS-dCas9-VP64 recoded; pCAG-NLS-dCas9-VP64
                     recoded CopyXXXXX;
                     pCAG-NLS-hCsy4-P2A-NLS-FLAG-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-NLS-hTtAgo (BPK633); pCAG-NLS-hTtAgo-NLS-3xFLAG
                     (BPK573); pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64;
                     pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64 D478A/D546A;
                     pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64 D478N/D546N;
                     pCAG-VP64-NLS-FLAG-NLS-dCas9-recoded-NLS-FLAG (BPK329);
                     pCAG-VP64-NLS-hTtAgo; pCAG-VP64-dCas9-recod linker variant
                     BbsI cassette; pCAG-VP64-dCas9-recoded GGGGSx5 linker;
                     pCAG-VP64-dCas9-recoded-NLS-FLAG (BPK278);
                     pCAG-VP64-hCsy4-NLS-P2A-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-dCas9-VP64;
                     pCAG-hCsy4-NLS-P2A-NLS-FLAG-NLS-hdCas9-NLS-FLAG-VP64
                     (BPK553);
                     pCAG-hCsy4-NLS-VP64-P2A-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-3xFLAG-KRAB
                     (BPK550); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64
                     delta175-307 (BPK544);
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded;
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK447);
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64-GG recoded
                     (BPK531); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-KRAB
                     (BPK454); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-VP64
                     recoded (BPK453); pCAG-hCsy4-P2A-NLS-dCas9-NLS-FLAG-VP64
                     recoded (BPK546); pCAG-hCsy4-P2A-NLS-dCas9-NLS-KRAB
                     (BPK548); pCAG-hCsy4-P2A-dCas9-NLS-FLAG-VP64 recoded
                     (BPK422); pCAG-hCsy4-P2A-dCas9-NLS-KRAB (BPK439);
                     pCAG-hCsy4-P2A-dCas9-NLS-VP64 recoded (BPK450);
                     pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK428); pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-KRAB
                     (BPK444); pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-VP64
                     recoded (BPK437); pCAG-hCsy4-T2A-dCas9-NLS-FLAG-VP64
                     recoded (BPK426); pCAG-hCsy4-T2A-dCas9-NLS-KRAB (BPK442);
                     pCAG-hCsy4-T2A-dCas9-NLS-VP64 recoded (BPK435);
                     pCAG-hCsy4-T2A-nls-hFokI-dhCas9-nls-3XFLAG (SQT1601);
                     pCAG-hTtAgo; pCAG-hTtAgo-NLS; pCAG-hTtAgo-NLS-3xFLAG;
                     pCAG-nls-FokI-Csy4-T2A-dCas9-recoded (N-terminal fusion
                     CK1891); pCMV-NLS-hTtAgo-NLS-3xFLAG; pCW-Cas9 (modified);
                     pCW-Cas9 Activator; pCW-Cas9 N-F-N Activator; pCW-Cas9
                     N-F-N Activator-GG; pCW-EFS-Puro-P2A-NFN-dCas9-VP64;
                     pCW-NLS-dCas9-VP64 EFS-Puro-T2A-rTta3-WPRE;
                     pEF1a-Csy4-T2A-NLS-FLAG-NLS-dCas9-VP64 recoded;
                     pEF1a-Csy4-T2A-dCas9-VP64 recoded;
                     pEF1a-Csy4-T2A-dCas9-VP64 recoded TRUNC;
                     pEF1a-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded;
                     pEFS-Csy4-T2A-dCas9-VP64 recoded;
                     pEFS-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK533); pLV-CMVEI.EGFPDS-iNpLTRSEBBA6_0693;
                     pLenti-TREminCMV-NLS-3xFLAG-NLS-dCas9-VP64
                     EFS-Puro-T2A-rTta3-WPRE; pLenti-TREminCMV-NLS-dCas9-VP64
                     EFS-Puro-T2A-rTta3-WPRE; pMLM3705; pMLM3705 annotation
                     mess; pWPXLd (modified); pcDNA5/FRT;
                     pcDNA5/FRT-tdtomato-AAVS1 site 1;
                     pcDNA5/FRT-tdtomato-AAVS1 site 1 in wrong way;
                     pcDNA5/FRT-tdtomato-AAVS1 site 2"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pCMV-T7-Cas9 (MLM3613), pCMV-T7-Cas9
                     delta NcoI"
                     /Transferred_Similarity="100.00%"
                     /modified_by="KleinstiverLab8"
                     /label="SV40_PA_terminator"
     misc_feature    complement(9345..16)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /X-Transferred_From="pBR322 origin"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pCAG-hCas9-nls-3XFLAG (SQT817),
                     pCAG-hCas9-nls-3XFLAG delta NcoI (BPK742), pCMV-T7-Cas9
                     (MLM3613), pCMV-T7-Cas9 delta NcoI"
                     /Transferred_Similarity="100.00%"
                     /label="pBR322 origin"
                     /note="Geneious type: origin of replication"
ORIGIN      
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gcgaaccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcgatctcc cgatccccta
     1201 gggtcgactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     1261 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     1321 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     1381 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
     1441 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     1501 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     1561 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     1621 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     1681 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     1741 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     1801 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     1861 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     1921 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     1981 agcagagctg gtttagtgaa ccgtcagatc cgctagagat ccgcggccgc taatacgact
     2041 cactataggg agagccgcca ccatgagcga gaagaaggaa atcgtgaagg gcagagattg
     2101 gaccttcctg gtgtaccccg aatctgctcc tgagaactgg cggaccatcc tcgatgagac
     2161 atttatgaga tgggtcgagt ccccactgca cgacaaggac gtgaacgccg acggcgagat
     2221 taagaaacct cattggcaca tcctgctgag cagcgacggc cccatcacac agaccgccgt
     2281 gcagaaaatc atcggccctc tgaacgcccc taatgcccag aaggtgggat ctgccaaggg
     2341 cctggttaga tatatggtgc acctggacaa ccccgaaaag taccaataca gcctggacga
     2401 gatcgtgggc cacaacggcg ccgatgtggc cagctacttc gagctgaccg cctctggcgg
     2461 aagcagcggc tccgaaaccc ctggcaccag cgagagcgct acacctgaat ccagcggagg
     2521 cagcgacaag aagtacagca tcggcctgga catcggcacc aactctgtgg gctgggccgt
     2581 gatcaccgac gagtacaagg tgcccagcaa gaaattcaag gtgctgggca acaccgaccg
     2641 gcacagcatc aagaagaacc tgatcggagc cctgctgttc gacagcggcg aaacagccga
     2701 ggccacccgg ctgaagagaa ccgccagaag aagatacacc agacggaaga accggatctg
     2761 ctatctgcaa gagatcttca gcaacgagat ggccaaggtg gacgacagct tcttccacag
     2821 actggaagag tccttcctgg tggaagagga taagaagcac gagcggcacc ccatcttcgg
     2881 caacatcgtg gacgaggtgg cctaccacga gaagtacccc accatctacc acctgagaaa
     2941 gaaactggtg gacagcaccg acaaggccga cctgcggctg atctatctgg ccctggccca
     3001 catgatcaag ttccggggcc acttcctgat cgagggcgac ctgaaccccg acaacagcga
     3061 cgtggacaag ctgttcatcc agctggtgca gacctacaac cagctgttcg aggaaaaccc
     3121 catcaacgcc agcggcgtgg acgccaaggc catcctgtct gccagactga gcaagagcag
     3181 acggctggaa aatctgatcg cccagctgcc cggcgagaag aagaatggcc tgttcggaaa
     3241 cctgattgcc ctgagcctgg gcctgacccc caacttcaag agcaacttcg acctggccga
     3301 ggatgccaaa ctgcagctga gcaaggacac ctacgacgac gacctggaca acctgctggc
     3361 ccagatcggc gaccagtacg ccgacctgtt tctggccgcc aagaacctgt ccgacgccat
     3421 cctgctgagc gacatcctga gagtgaacac cgagatcacc aaggcccccc tgagcgcctc
     3481 tatgatcaag agatacgacg agcaccacca ggacctgacc ctgctgaaag ctctcgtgcg
     3541 gcagcagctg cctgagaagt acaaagagat tttcttcgac cagagcaaga acggctacgc
     3601 cggctacatt gacggcggag ccagccagga agagttctac aagttcatca agcccatcct
     3661 ggaaaagatg gacggcaccg aggaactgct cgtgaagctg aacagagagg acctgctgcg
     3721 gaagcagcgg accttcgaca acggcagcat cccccaccag atccacctgg gagagctgca
     3781 cgccattctg cggcggcagg aagattttta cccattcctg aaggacaacc gggaaaagat
     3841 cgagaagatc ctgaccttcc gcatccccta ctacgtgggc cctctggcca ggggaaacag
     3901 cagattcgcc tggatgacca gaaagagcga ggaaaccatc accccctgga acttcgagga
     3961 agtggtggac aagggcgctt ccgcccagag cttcatcgag cggatgacca acttcgataa
     4021 gaacctgccc aacgagaagg tgctgcccaa gcacagcctg ctgtacgagt acttcaccgt
     4081 gtataacgag ctgaccaaag tgaaatacgt gaccgaggga atgagaaagc ccgccttcct
     4141 gagcggcgag cagaaaaagg ccatcgtgga cctgctgttc aagaccaacc ggaaagtgac
     4201 cgtgaagcag ctgaaagagg actacttcaa gaaaatcgag tgcttcgact ccgtggaaat
     4261 ctccggcgtg gaagatcggt tcaacgcctc cctgggcaca taccacgatc tgctgaaaat
     4321 tatcaaggac aaggacttcc tggacaatga ggaaaacgag gacattctgg aagatatcgt
     4381 gctgaccctg acactgtttg aggacagaga gatgatcgag gaacggctga aaacctatgc
     4441 ccacctgttc gacgacaaag tgatgaagca gctgaagcgg cggagataca ccggctgggg
     4501 caggctgagc cggaagctga tcaacggcat ccgggacaag cagtccggca agacaatcct
     4561 ggatttcctg aagtccgacg gcttcgccaa cagaaacttc atgcagctga tccacgacga
     4621 cagcctgacc tttaaagagg acatccagaa agcccaggtg tccggccagg gcgatagcct
     4681 gcacgagcac attgccaatc tggccggcag ccccgccatt aagaagggca tcctgcagac
     4741 agtgaaggtg gtggacgagc tcgtgaaagt gatgggccgg cacaagcccg agaacatcgt
     4801 gatcgaaatg gccagagaga accagaccac ccagaaggga cagaagaaca gccgcgagag
     4861 aatgaagcgg atcgaagagg gcatcaaaga gctgggcagc cagatcctga aagaacaccc
     4921 cgtggaaaac acccagctgc agaacgagaa gctgtacctg tactacctgc agaatgggcg
     4981 ggatatgtac gtggaccagg aactggacat caaccggctg tccgactacg atgtggacgc
     5041 tatcgtgcct cagagctttc tgaaggacga ctccatcgac aacaaggtgc tgaccagaag
     5101 cgacaagaac cggggcaaga gcgacaacgt gccctccgaa gaggtcgtga agaagatgaa
     5161 gaactactgg cggcagctgc tgaacgccaa gctgattacc cagagaaagt tcgacaatct
     5221 gaccaaggcc gagagaggcg gcctgagcga actggataag gccggcttca tcaagagaca
     5281 gctggtggaa acccggcaga tcacaaagca cgtggcacag atcctggact cccggatgaa
     5341 cactaagtac gacgagaatg acaagctgat ccgggaagtg aaagtgatca ccctgaagtc
     5401 caagctggtg tccgatttcc ggaaggattt ccagttttac aaagtgcgcg agatcaacaa
     5461 ctaccaccac gcccacgacg cctacctgaa cgccgtcgtg ggaaccgccc tgatcaaaaa
     5521 gtaccctaag ctggaaagcg agttcgtgta cggcgactac aaggtgtacg acgtgcggaa
     5581 gatgatcgcc aagagcgagc aggaaatcgg caaggctacc gccaagtact tcttctacag
     5641 caacatcatg aactttttca agaccgagat taccctggcc aacggcgaga tccggaagcg
     5701 gcctctgatc gagacaaacg gcgaaaccgg ggagatcgtg tgggataagg gccgggattt
     5761 tgccaccgtg cggaaagtgc tgagcatgcc ccaagtgaat atcgtgaaaa agaccgaggt
     5821 gcagacaggc ggcttcagca aagagtctat cctgcccaag aggaacagcg ataagctgat
     5881 cgccagaaag aaggactggg accctaagaa gtacggcggc ttcgacagcc ccaccgtggc
     5941 ctattctgtg ctggtggtgg ccaaagtgga aaagggcaag tccaagaaac tgaagagtgt
     6001 gaaagagctg ctggggatca ccatcatgga aagaagcagc ttcgagaaga atcccatcga
     6061 ctttctggaa gccaagggct acaaagaagt gaaaaaggac ctgatcatca agctgcctaa
     6121 gtactccctg ttcgagctgg aaaacggccg gaagagaatg ctggcctctg ccggcgaact
     6181 gcagaaggga aacgaactgg ccctgccctc caaatatgtg aacttcctgt acctggccag
     6241 ccactatgag aagctgaagg gctcccccga ggataatgag cagaaacagc tgtttgtgga
     6301 acagcacaag cactacctgg acgagatcat cgagcagatc agcgagttct ccaagagagt
     6361 gatcctggcc gacgctaatc tggacaaagt gctgtccgcc tacaacaagc accgggataa
     6421 gcccatcaga gagcaggccg agaatatcat ccacctgttt accctgacca atctgggagc
     6481 ccctgccgcc ttcaagtact ttgacaccac catcgaccgg aagaggtaca ccagcaccaa
     6541 agaggtgctg gacgccaccc tgatccacca gagcatcacc ggcctgtacg agacacggat
     6601 cgacctgtct cagctgggag gtgactccgg cggaagctct ggtggcagca agcggaccgc
     6661 cgacggctct gaattcgaga gccctaagaa gaaaagaaag gtgagcggag gctctagcgg
     6721 cggaagcgtt atctcctacg acaactacgt gaccatcctg gacgaagaga cactgaaggc
     6781 ttggatcgct aaactggaaa aagcccctgt gttcgccttc gccaccgcca ccgactcctt
     6841 agataacatc agcgccaacc tggtgggcct gtctttcgcc atcgagcctg gcgtggccgc
     6901 ctacatcccc gtggcccacg attacctgga tgccccagat caaatcagca gagagagagc
     6961 actggagctc ctgaagcccc tgctggagga cgagaaggcc ctgaaggtgg gacagaacct
     7021 gaaatacgat aggggaatcc tggctaatta cggcatcgag ctgagaggca tcgccttcga
     7081 taccatgctg gaatcttaca tcctgaacag cgtggccggc cgccacgaca tggacagcct
     7141 ggctgaacgg tggctgaagc acaaaaccat caccttcgaa gagatcgccg gaaagggcaa
     7201 gaaccagctg acatttaatc agatcgcact ggaagaagcc ggcagatacg ccgctgagga
     7261 cgccgacgtg acccttcagc tgcacctgaa gatgtggccc gatctgcaga agcacaaggg
     7321 cccactaaac gtgttcgaga acatcgagat gcctctggtg cccgtcctga gcagaatcga
     7381 gaggaatggc gtgaagatcg accccaaggt gctgcacaac cacagcgagg aactgacact
     7441 gcggctggcc gagctggaaa agaaggccca cgaaatcgcc ggcgaggagt tcaacctgtc
     7501 tagcaccaag cagctacaaa caatcctgtt cgagaagcag ggcattaagc ctctgaaaaa
     7561 gacacctggc ggagccccca gcaccagcga agaagtgctg gaggaactgg cgctggatta
     7621 ccctctgccc aaagtgatcc tggaatatag aggactggcc aagctgaagt ctacatacac
     7681 cgacaagctg cctctgatga tcaaccccaa gaccggccgg gtgcatacat cttatcacca
     7741 ggccgtgaca gccaccggcc ggctgagcag cacagatcct aacctgcaaa acatcccagt
     7801 gcggaacgaa gagggcagaa gaatcagaca ggctttcatt gcccctgagg actacgtcat
     7861 cgtgtctgct gactacagcc agatagaact gagaatcatg gcccatctgt ctcgggacaa
     7921 gggcctgctg accgcttttg cagaaggcaa ggacatccac agagccaccg ctgccgaagt
     7981 gttcggcctg cctctggaaa cagtgaccag cgagcaaaga aggtccgcca aggccattaa
     8041 cttcggcctg atctacggca tgagcgcctt tggcctggcc agacagctca acatccctag
     8101 aaaggaggcc cagaaataca tggacctgta cttcgagcgg taccccggcg tgctggagta
     8161 catggaacgg accagagccc aggctaaaga gcagggatac gtggaaaccc tggatggccg
     8221 gagactgtat ctgcctgaca tcaagagcag caacggcgcc agaagagccg ccgctgagag
     8281 agctgctatc aacgccccta tgcagggcac cgccgccgac atcatcaagc gggccatgat
     8341 cgccgtggac gcctggctgc aggccgagca gcctagagtg cggatgatca tgcaggtgca
     8401 cgacgagctg gtgtttgagg tgcacaagga cgacgtggat gctgtggcca agcagattca
     8461 ccagctgatg gaaaattgca ccagactgga cgtgccactg ctggtcgagg ttggaagcgg
     8521 agagaattgg gaccaggccc actctggcgg ctcaaaaaga accgccgacg gcagcgaatt
     8581 cgagtctccc aagaagaaga ggaaagtctg accggtcatc atcaccatca ccattgagtt
     8641 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     8701 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     8761 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     8821 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     8881 tctatggctt ctgaggcgga aagaaccagc tggggctcga taccgtcgac ctctagctag
     8941 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     9001 ccacacaaca tacgagccgg aagcataaag tgtaaagcct agggtgccta atgagtgagc
     9061 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     9121 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     9181 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     9241 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     9301 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     9361 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     9421 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     9481 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     9541 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     9601 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     9661 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     9721 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     9781 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     9841 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     9901 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaa
//
