LOCUS       pCMV-T7-B103_(LM2907)        5196 bp    DNA     circular SYN 24-NOV-2023
DEFINITION  JDS246.
ACCESSION   urn.local...118-h0aqq87
KEYWORDS    .
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     enhancer        132..356
                     /created_by="lha16"
                     /modified_by="lha16"
                     /label="CMV_promoter"
     promoter        377..396
                     /X-Transferred_From="pCRII-TOPO"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pCMV-T7-Cas9 (MLM3613), pCMV-T7-Cas9
                     delta NcoI"
                     /Transferred_Similarity="100.00%"
                     /modified_by="lha16"
                     /label="T7 promoter"
     CDS             412..2124
                     /created_by="lha16"
                     /Color="#ee0077"
                     /modified_by="lha16"
                     /label="B103(H73R, A147K, R221Y, A318G, M339L, E359D,
                     K372E, F383L, D384N, A503M, I511V, R544K, T550K)"
     CDS             625..627
                     /created_by="lha16"
                     /label="H73R"
     CDS             847..849
                     /created_by="lha16"
                     /label="A147K"
     CDS             1069..1071
                     /created_by="lha16"
                     /label="R221Y"
     CDS             1360..1362
                     /created_by="lha16"
                     /label="A318G"
     CDS             1423..1425
                     /created_by="lha16"
                     /label="M339L"
     CDS             1483..1485
                     /created_by="lha16"
                     /label="E359D"
     CDS             1522..1524
                     /created_by="lha16"
                     /label="K372E"
     CDS             1555..1557
                     /created_by="lha16"
                     /label="F383L"
     CDS             1558..1560
                     /created_by="lha16"
                     /label="D384N"
     CDS             1915..1917
                     /created_by="lha16"
                     /label="A503M"
     CDS             1939..1941
                     /created_by="lha16"
                     /label="I511V"
     CDS             2038..2040
                     /created_by="lha16"
                     /modified_by="lha16"
                     /label="R544K"
     CDS             2056..2058
                     /created_by="lha16"
                     /label="T550K"
     CDS             2125..2148
                     /Color="#ff7400"
                     /created_by="lha16"
                     /label="GS linker"
     CDS             2149..2202
                     /Color="#6bba70"
                     /created_by="lha16"
                     /label="BPNLS"
     CDS             2203..2205
                     /Color="#000000"
                     /created_by="lha16"
                     /label
     terminator      2254..2481
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIK
                     KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
                     ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH
                     MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSK
                     SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD
                     NLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTL
                     LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVK
                     LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
                     YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL
                     PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKE
                     DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT
                     LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF
                     LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT
                     VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKE
                     HPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKV
                     LTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA
                     GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG
                     KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
                     MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV
                     AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLF
                     ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH
                     KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA
                     PAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDGSPKKKRKVS
                     SDYKDHDGDYKDHDIDYKDDDDKAAG*"
                     /label="bGH_PA_terminator"
     terminator      2740..2871
                     /SerialCloner_Color="&hF08080"
                     /SerialCloner_Show="False"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""SV40_PA_terminator"" -
                     translation=""MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     -
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     -
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     - GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLST \"
                     /X-Transferred_From="3.
                     pCAG-nls-Csy4-FokI-T2A-dCas9-recoded (C-terminal fusion) -
                     linker variants; 6.pCAG-nls-FokI-Csy4-T2A-Cas9-recoded
                     (N-terminal fusion) -linker variants; JDS246;
                     VC-(VC780+Csy4-Cas9)_pLKO-TRC001;
                     pCAG-Csy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK376); pCAG-Csy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64
                     recoded (BPK376frameshift);
                     pCAG-Csy4-P2A-dCas9-NLS-FLAG-VP64 recoded;
                     pCAG-Csy4-T2A-Cas9-VP64 recoded (BPK1);
                     pCAG-Csy4-T2A-Cas9-VP64 recoded (BPK2a);
                     pCAG-Csy4-T2A-Cas9-recoded (SQT834);
                     pCAG-Csy4-T2A-FokI-dCas9-recoded-nls (JAF1484);
                     pCAG-Csy4-T2A-KRAB-dCas9-recoded-nlsFLAG (NW9);
                     pCAG-Csy4-T2A-NLS-FLAG-NLS-VP64-dCas9-recoded (making
                     p125); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-KRAB recoded
                     (BPK353); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-VP64 recoded
                     (BPK326); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-VP64 recoded NO
                     CTERM FLAG;
                     pCAG-Csy4-T2A-VP64-NLS/FLAG/NLS-dCas9-recoded-nlsFLAG
                     (BPK328); pCAG-Csy4-T2A-VP64-dCas9 linker variant BbsI
                     cassette; pCAG-Csy4-T2A-VP64-dCas9-recoded-nlsFLAG
                     (BPK285); pCAG-Csy4-T2A-VP64-dCas9-recoded-nlsFLAG
                     (BPK286); pCAG-Csy4-T2A-dCas9-KRAB recoded (NW8);
                     pCAG-Csy4-T2A-dCas9-VP64 recoded (BPK3);
                     pCAG-Csy4-T2A-dCas9-VP64 recoded NO FLAG (BPK373 or 374?);
                     pCAG-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64;
                     pCAG-NLS-FLAG-NLS-dCas9-VP64 recoded;
                     pCAG-NLS-FLAG-VP64-dCas9-recoded;
                     pCAG-NLS-VP64-dCas9-recoded; pCAG-NLS-dCas9-NLS-3xFLAG
                     recoded; pCAG-NLS-dCas9-VP64 recoded; pCAG-NLS-dCas9-VP64
                     recoded CopyXXXXX;
                     pCAG-NLS-hCsy4-P2A-NLS-FLAG-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-NLS-hTtAgo (BPK633); pCAG-NLS-hTtAgo-NLS-3xFLAG
                     (BPK573); pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64;
                     pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64 D478A/D546A;
                     pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64 D478N/D546N;
                     pCAG-VP64-NLS-FLAG-NLS-dCas9-recoded-NLS-FLAG (BPK329);
                     pCAG-VP64-NLS-hTtAgo; pCAG-VP64-dCas9-recod linker variant
                     BbsI cassette; pCAG-VP64-dCas9-recoded GGGGSx5 linker;
                     pCAG-VP64-dCas9-recoded-NLS-FLAG (BPK278);
                     pCAG-VP64-hCsy4-NLS-P2A-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-dCas9-VP64;
                     pCAG-hCsy4-NLS-P2A-NLS-FLAG-NLS-hdCas9-NLS-FLAG-VP64
                     (BPK553);
                     pCAG-hCsy4-NLS-VP64-P2A-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-3xFLAG-KRAB
                     (BPK550); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64
                     delta175-307 (BPK544);
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded;
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK447);
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64-GG recoded
                     (BPK531); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-KRAB
                     (BPK454); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-VP64
                     recoded (BPK453); pCAG-hCsy4-P2A-NLS-dCas9-NLS-FLAG-VP64
                     recoded (BPK546); pCAG-hCsy4-P2A-NLS-dCas9-NLS-KRAB
                     (BPK548); pCAG-hCsy4-P2A-dCas9-NLS-FLAG-VP64 recoded
                     (BPK422); pCAG-hCsy4-P2A-dCas9-NLS-KRAB (BPK439);
                     pCAG-hCsy4-P2A-dCas9-NLS-VP64 recoded (BPK450);
                     pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK428); pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-KRAB
                     (BPK444); pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-VP64
                     recoded (BPK437); pCAG-hCsy4-T2A-dCas9-NLS-FLAG-VP64
                     recoded (BPK426); pCAG-hCsy4-T2A-dCas9-NLS-KRAB (BPK442);
                     pCAG-hCsy4-T2A-dCas9-NLS-VP64 recoded (BPK435);
                     pCAG-hCsy4-T2A-nls-hFokI-dhCas9-nls-3XFLAG (SQT1601);
                     pCAG-hTtAgo; pCAG-hTtAgo-NLS; pCAG-hTtAgo-NLS-3xFLAG;
                     pCAG-nls-FokI-Csy4-T2A-dCas9-recoded (N-terminal fusion
                     CK1891); pCMV-NLS-hTtAgo-NLS-3xFLAG; pCW-Cas9 (modified);
                     pCW-Cas9 Activator; pCW-Cas9 N-F-N Activator; pCW-Cas9
                     N-F-N Activator-GG; pCW-EFS-Puro-P2A-NFN-dCas9-VP64;
                     pCW-NLS-dCas9-VP64 EFS-Puro-T2A-rTta3-WPRE;
                     pEF1a-Csy4-T2A-NLS-FLAG-NLS-dCas9-VP64 recoded;
                     pEF1a-Csy4-T2A-dCas9-VP64 recoded;
                     pEF1a-Csy4-T2A-dCas9-VP64 recoded TRUNC;
                     pEF1a-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded;
                     pEFS-Csy4-T2A-dCas9-VP64 recoded;
                     pEFS-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK533); pLV-CMVEI.EGFPDS-iNpLTRSEBBA6_0693;
                     pLenti-TREminCMV-NLS-3xFLAG-NLS-dCas9-VP64
                     EFS-Puro-T2A-rTta3-WPRE; pLenti-TREminCMV-NLS-dCas9-VP64
                     EFS-Puro-T2A-rTta3-WPRE; pMLM3705; pMLM3705 annotation
                     mess; pWPXLd (modified); pcDNA5/FRT;
                     pcDNA5/FRT-tdtomato-AAVS1 site 1;
                     pcDNA5/FRT-tdtomato-AAVS1 site 1 in wrong way;
                     pcDNA5/FRT-tdtomato-AAVS1 site 2"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pCMV-T7-Cas9 (MLM3613), pCMV-T7-Cas9
                     delta NcoI"
                     /Transferred_Similarity="100.00%"
                     /modified_by="KleinstiverLab8"
                     /label="SV40_PA_terminator"
     misc_feature    complement(2939..3558)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /X-Transferred_From="pBR322 origin"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pCAG-hCas9-nls-3XFLAG (SQT817),
                     pCAG-hCas9-nls-3XFLAG delta NcoI (BPK742), pCMV-T7-Cas9
                     (MLM3613), pCMV-T7-Cas9 delta NcoI"
                     /Transferred_Similarity="100.00%"
                     /label="pBR322 origin"
                     /note="Geneious type: origin of replication"
     CDS             complement(3713..4573)
                     /gene="Ampicillin"
                     /Original_Translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVL
                     GNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKV
                     DDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADL
                     RLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAK
                     AILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLS
                     KDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRY
                     DEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKM
                     DGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIE
                     KILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFD
                     KNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNR
                     KVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDI
                     LEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDK
                     QSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSP
                     AIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIK
                     ELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFL
                     KDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAER
                     GGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLV
                     SDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKM
                     IAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRD
                     FATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSP
                     TVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLI
                     IKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNE
                     QKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIH
                     LFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDG
                     SPKKKRKVSSDYKDHDGDYKDHDIDYKDDDDKAAG*"
                     /Color="#777777"
                     /modified_by="lha16"
                     /label="Ampicillin"
     promoter        complement(4574..4678)
                     /X-Transferred_From="JDS246; pCMV-NLS-hTtAgo-NLS-3xFLAG;
                     pLV-CMVEI.EGFPDS-iNpLTRSEBBA6_0693; pLVX-TetOne-puro;
                     pMLM3705; pMLM3705 annotation mess"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pCMV-T7-Cas9 (MLM3613), pCMV-T7-Cas9
                     delta NcoI"
                     /Transferred_Similarity="100.00%"
                     /modified_by="lha16"
                     /label="AmpR promoter"
     promoter        4962..131
                     /SerialCloner_Color="&h03C000"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /X-Transferred_From="3.
                     pCAG-nls-Csy4-FokI-T2A-dCas9-recoded (C-terminal fusion) -
                     linker variants; 6.pCAG-nls-FokI-Csy4-T2A-Cas9-recoded
                     (N-terminal fusion) -linker variants; JDS246;
                     pCAG-Csy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK376); pCAG-Csy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64
                     recoded (BPK376frameshift);
                     pCAG-Csy4-P2A-dCas9-NLS-FLAG-VP64 recoded;
                     pCAG-Csy4-T2A-Cas9-VP64 recoded (BPK1);
                     pCAG-Csy4-T2A-Cas9-VP64 recoded (BPK2a);
                     pCAG-Csy4-T2A-Cas9-recoded (SQT834);
                     pCAG-Csy4-T2A-FokI-dCas9-recoded-nls (JAF1484);
                     pCAG-Csy4-T2A-KRAB-dCas9-recoded-nlsFLAG (NW9);
                     pCAG-Csy4-T2A-NLS-FLAG-NLS-VP64-dCas9-recoded (making
                     p125); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-KRAB recoded
                     (BPK353); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-VP64 recoded
                     (BPK326); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-VP64 recoded NO
                     CTERM FLAG;
                     pCAG-Csy4-T2A-VP64-NLS/FLAG/NLS-dCas9-recoded-nlsFLAG
                     (BPK328); pCAG-Csy4-T2A-VP64-dCas9 linker variant BbsI
                     cassette; pCAG-Csy4-T2A-VP64-dCas9-recoded-nlsFLAG
                     (BPK285); pCAG-Csy4-T2A-VP64-dCas9-recoded-nlsFLAG
                     (BPK286); pCAG-Csy4-T2A-dCas9-KRAB recoded (NW8);
                     pCAG-Csy4-T2A-dCas9-VP64 recoded (BPK3);
                     pCAG-Csy4-T2A-dCas9-VP64 recoded NO FLAG (BPK373 or 374?);
                     pCAG-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64;
                     pCAG-NLS-FLAG-NLS-dCas9-VP64 recoded;
                     pCAG-NLS-FLAG-VP64-dCas9-recoded;
                     pCAG-NLS-VP64-dCas9-recoded; pCAG-NLS-dCas9-NLS-3xFLAG
                     recoded; pCAG-NLS-dCas9-VP64 recoded; pCAG-NLS-dCas9-VP64
                     recoded CopyXXXXX;
                     pCAG-NLS-hCsy4-P2A-NLS-FLAG-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-NLS-hTtAgo (BPK633); pCAG-NLS-hTtAgo-NLS-3xFLAG
                     (BPK573); pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64;
                     pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64 D478A/D546A;
                     pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64 D478N/D546N;
                     pCAG-VP64-NLS-FLAG-NLS-dCas9-recoded-NLS-FLAG (BPK329);
                     pCAG-VP64-NLS-hTtAgo; pCAG-VP64-dCas9-recod linker variant
                     BbsI cassette; pCAG-VP64-dCas9-recoded GGGGSx5 linker;
                     pCAG-VP64-dCas9-recoded-NLS-FLAG (BPK278);
                     pCAG-VP64-hCsy4-NLS-P2A-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-dCas9-VP64;
                     pCAG-hCsy4-NLS-P2A-NLS-FLAG-NLS-hdCas9-NLS-FLAG-VP64
                     (BPK553);
                     pCAG-hCsy4-NLS-VP64-P2A-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-3xFLAG-KRAB
                     (BPK550); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64
                     delta175-307 (BPK544);
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded;
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK447);
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64-GG recoded
                     (BPK531); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-KRAB
                     (BPK454); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-VP64
                     recoded (BPK453); pCAG-hCsy4-P2A-NLS-dCas9-NLS-FLAG-VP64
                     recoded (BPK546); pCAG-hCsy4-P2A-NLS-dCas9-NLS-KRAB
                     (BPK548); pCAG-hCsy4-P2A-dCas9-NLS-FLAG-VP64 recoded
                     (BPK422); pCAG-hCsy4-P2A-dCas9-NLS-KRAB (BPK439);
                     pCAG-hCsy4-P2A-dCas9-NLS-VP64 recoded (BPK450);
                     pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK428); pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-KRAB
                     (BPK444); pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-VP64
                     recoded (BPK437); pCAG-hCsy4-T2A-dCas9-NLS-FLAG-VP64
                     recoded (BPK426); pCAG-hCsy4-T2A-dCas9-NLS-KRAB (BPK442);
                     pCAG-hCsy4-T2A-dCas9-NLS-VP64 recoded (BPK435);
                     pCAG-hCsy4-T2A-nls-hFokI-dhCas9-nls-3XFLAG (SQT1601);
                     pCAG-hTtAgo; pCAG-hTtAgo-NLS; pCAG-hTtAgo-NLS-3xFLAG;
                     pCAG-nls-FokI-Csy4-T2A-dCas9-recoded (N-terminal fusion
                     CK1891); pCMV-NLS-hTtAgo-NLS-3xFLAG; pLenti-CAG-Puro-DEST;
                     pMLM3705; pMLM3705 annotation mess"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pCMV-T7-Cas9 (MLM3613), pCMV-T7-Cas9
                     delta NcoI"
                     /Transferred_Similarity="100.00%"
                     /modified_by="lha16"
                     /label="CMV Enhancer"
     promoter        complement(5011..5039)
                     /SerialCloner_Color="&hF08080"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""AmpR_promoter"" -
                     translation=""MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     -
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     -
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     - DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWM \"
                     /X-Transferred_From="3.
                     pCAG-nls-Csy4-FokI-T2A-dCas9-recoded (C-terminal fusion) -
                     linker variants; 6.pCAG-nls-FokI-Csy4-T2A-Cas9-recoded
                     (N-terminal fusion) -linker variants; JDS246;
                     pCAG-Csy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK376); pCAG-Csy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64
                     recoded (BPK376frameshift);
                     pCAG-Csy4-P2A-dCas9-NLS-FLAG-VP64 recoded;
                     pCAG-Csy4-T2A-Cas9-VP64 recoded (BPK1);
                     pCAG-Csy4-T2A-Cas9-VP64 recoded (BPK2a);
                     pCAG-Csy4-T2A-Cas9-recoded (SQT834);
                     pCAG-Csy4-T2A-FokI-dCas9-recoded-nls (JAF1484);
                     pCAG-Csy4-T2A-KRAB-dCas9-recoded-nlsFLAG (NW9);
                     pCAG-Csy4-T2A-NLS-FLAG-NLS-VP64-dCas9-recoded (making
                     p125); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-KRAB recoded
                     (BPK353); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-VP64 recoded
                     (BPK326); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-VP64 recoded NO
                     CTERM FLAG;
                     pCAG-Csy4-T2A-VP64-NLS/FLAG/NLS-dCas9-recoded-nlsFLAG
                     (BPK328); pCAG-Csy4-T2A-VP64-dCas9 linker variant BbsI
                     cassette; pCAG-Csy4-T2A-VP64-dCas9-recoded-nlsFLAG
                     (BPK285); pCAG-Csy4-T2A-VP64-dCas9-recoded-nlsFLAG
                     (BPK286); pCAG-Csy4-T2A-dCas9-KRAB recoded (NW8);
                     pCAG-Csy4-T2A-dCas9-VP64 recoded (BPK3);
                     pCAG-Csy4-T2A-dCas9-VP64 recoded NO FLAG (BPK373 or 374?);
                     pCAG-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64;
                     pCAG-NLS-FLAG-NLS-dCas9-VP64 recoded;
                     pCAG-NLS-FLAG-VP64-dCas9-recoded;
                     pCAG-NLS-VP64-dCas9-recoded; pCAG-NLS-dCas9-NLS-3xFLAG
                     recoded; pCAG-NLS-dCas9-VP64 recoded; pCAG-NLS-dCas9-VP64
                     recoded CopyXXXXX;
                     pCAG-NLS-hCsy4-P2A-NLS-FLAG-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-NLS-hTtAgo (BPK633); pCAG-NLS-hTtAgo-NLS-3xFLAG
                     (BPK573); pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64;
                     pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64 D478A/D546A;
                     pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64 D478N/D546N;
                     pCAG-VP64-NLS-FLAG-NLS-dCas9-recoded-NLS-FLAG (BPK329);
                     pCAG-VP64-NLS-hTtAgo; pCAG-VP64-dCas9-recod linker variant
                     BbsI cassette; pCAG-VP64-dCas9-recoded GGGGSx5 linker;
                     pCAG-VP64-dCas9-recoded-NLS-FLAG (BPK278);
                     pCAG-VP64-hCsy4-NLS-P2A-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-dCas9-VP64;
                     pCAG-hCsy4-NLS-P2A-NLS-FLAG-NLS-hdCas9-NLS-FLAG-VP64
                     (BPK553);
                     pCAG-hCsy4-NLS-VP64-P2A-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-3xFLAG-KRAB
                     (BPK550); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64
                     delta175-307 (BPK544);
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded;
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK447);
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64-GG recoded
                     (BPK531); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-KRAB
                     (BPK454); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-VP64
                     recoded (BPK453); pCAG-hCsy4-P2A-NLS-dCas9-NLS-FLAG-VP64
                     recoded (BPK546); pCAG-hCsy4-P2A-NLS-dCas9-NLS-KRAB
                     (BPK548); pCAG-hCsy4-P2A-dCas9-NLS-FLAG-VP64 recoded
                     (BPK422); pCAG-hCsy4-P2A-dCas9-NLS-KRAB (BPK439);
                     pCAG-hCsy4-P2A-dCas9-NLS-VP64 recoded (BPK450);
                     pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK428); pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-KRAB
                     (BPK444); pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-VP64
                     recoded (BPK437); pCAG-hCsy4-T2A-dCas9-NLS-FLAG-VP64
                     recoded (BPK426); pCAG-hCsy4-T2A-dCas9-NLS-KRAB (BPK442);
                     pCAG-hCsy4-T2A-dCas9-NLS-VP64 recoded (BPK435);
                     pCAG-hCsy4-T2A-nls-hFokI-dhCas9-nls-3XFLAG (SQT1601);
                     pCAG-hTtAgo; pCAG-hTtAgo-NLS; pCAG-hTtAgo-NLS-3xFLAG;
                     pCAG-nls-FokI-Csy4-T2A-dCas9-recoded (N-terminal fusion
                     CK1891); pCMV-NLS-hTtAgo-NLS-3xFLAG; pLenti-CAG-Puro-DEST;
                     pMLM3705; pMLM3705 annotation mess; pcDNA5/FRT"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pCMV-T7-Cas9 (MLM3613), pCMV-T7-Cas9
                     delta NcoI"
                     /Transferred_Similarity="100.00%"
                     /label="AmpR_promoter"
     promoter        5028..119
                     /SerialCloner_Color="&hDC143C"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""CAG_enhancer"" - 84..371 \"
                     /X-Transferred_From="3.
                     pCAG-nls-Csy4-FokI-T2A-dCas9-recoded (C-terminal fusion) -
                     linker variants; 6.pCAG-nls-FokI-Csy4-T2A-Cas9-recoded
                     (N-terminal fusion) -linker variants; JDS246;
                     pCAG-AttB-Csy4-VEGFA1-3 (BPK318); pCAG-CFP;
                     pCAG-Csy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK376); pCAG-Csy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64
                     recoded (BPK376frameshift);
                     pCAG-Csy4-P2A-dCas9-NLS-FLAG-VP64 recoded;
                     pCAG-Csy4-T2A-Cas9-VP64 recoded (BPK1);
                     pCAG-Csy4-T2A-Cas9-VP64 recoded (BPK2a);
                     pCAG-Csy4-T2A-Cas9-recoded (SQT834);
                     pCAG-Csy4-T2A-FokI-dCas9-recoded-nls (JAF1484);
                     pCAG-Csy4-T2A-KRAB-dCas9-recoded-nlsFLAG (NW9);
                     pCAG-Csy4-T2A-NLS-FLAG-NLS-VP64-dCas9-recoded (making
                     p125); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-KRAB recoded
                     (BPK353); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-VP64 recoded
                     (BPK326); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-VP64 recoded NO
                     CTERM FLAG;
                     pCAG-Csy4-T2A-VP64-NLS/FLAG/NLS-dCas9-recoded-nlsFLAG
                     (BPK328); pCAG-Csy4-T2A-VP64-dCas9 linker variant BbsI
                     cassette; pCAG-Csy4-T2A-VP64-dCas9-recoded-nlsFLAG
                     (BPK285); pCAG-Csy4-T2A-VP64-dCas9-recoded-nlsFLAG
                     (BPK286); pCAG-Csy4-T2A-dCas9-KRAB recoded (NW8);
                     pCAG-Csy4-T2A-dCas9-VP64 recoded (BPK3);
                     pCAG-Csy4-T2A-dCas9-VP64 recoded NO FLAG (BPK373 or 374?);
                     pCAG-Csy4site2-gRNA-Csy4site2 (SQT1468) + 1 site;
                     pCAG-Csy4site2-gRNA-Csy4site2 (SQT1468) + 4 sites;
                     pCAG-Csy4site2-gRNA-Csy4site2 BsaI;
                     pCAG-Csy4site2-gRNA-Csy4site2 BsaI cassette; pCAG-DEST
                     (BPK88); pCAG-DEST V2;
                     pCAG-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64;
                     pCAG-NLS-FLAG-NLS-dCas9-VP64 recoded;
                     pCAG-NLS-FLAG-VP64-dCas9-recoded;
                     pCAG-NLS-VP64-dCas9-recoded; pCAG-NLS-dCas9-NLS-3xFLAG
                     recoded; pCAG-NLS-dCas9-VP64 recoded; pCAG-NLS-dCas9-VP64
                     recoded CopyXXXXX;
                     pCAG-NLS-hCsy4-P2A-NLS-FLAG-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-NLS-hTtAgo (BPK633); pCAG-NLS-hTtAgo-NLS-3xFLAG
                     (BPK573); pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64;
                     pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64 D478A/D546A;
                     pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64 D478N/D546N;
                     pCAG-VP64-NLS-FLAG-NLS-dCas9-recoded-NLS-FLAG (BPK329);
                     pCAG-VP64-NLS-hTtAgo; pCAG-VP64-dCas9-recod linker variant
                     BbsI cassette; pCAG-VP64-dCas9-recoded GGGGSx5 linker;
                     pCAG-VP64-dCas9-recoded-NLS-FLAG (BPK278);
                     pCAG-VP64-hCsy4-NLS-P2A-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-dCas9-VP64;
                     pCAG-hCsy4-NLS-P2A-NLS-FLAG-NLS-hdCas9-NLS-FLAG-VP64
                     (BPK553);
                     pCAG-hCsy4-NLS-VP64-P2A-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-3xFLAG-KRAB
                     (BPK550); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64
                     delta175-307 (BPK544);
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded;
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK447);
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64-GG recoded
                     (BPK531); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-KRAB
                     (BPK454); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-VP64
                     recoded (BPK453); pCAG-hCsy4-P2A-NLS-dCas9-NLS-FLAG-VP64
                     recoded (BPK546); pCAG-hCsy4-P2A-NLS-dCas9-NLS-KRAB
                     (BPK548); pCAG-hCsy4-P2A-dCas9-NLS-FLAG-VP64 recoded
                     (BPK422); pCAG-hCsy4-P2A-dCas9-NLS-KRAB (BPK439);
                     pCAG-hCsy4-P2A-dCas9-NLS-VP64 recoded (BPK450);
                     pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK428); pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-KRAB
                     (BPK444); pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-VP64
                     recoded (BPK437); pCAG-hCsy4-T2A-dCas9-NLS-FLAG-VP64
                     recoded (BPK426); pCAG-hCsy4-T2A-dCas9-NLS-KRAB (BPK442);
                     pCAG-hCsy4-T2A-dCas9-NLS-VP64 recoded (BPK435);
                     pCAG-hCsy4-T2A-nls-hFokI-dhCas9-nls-3XFLAG (SQT1601);
                     pCAG-hTtAgo; pCAG-hTtAgo-NLS; pCAG-hTtAgo-NLS-3xFLAG;
                     pCAG-nls-FokI-Csy4-T2A-dCas9-recoded (N-terminal fusion
                     CK1891); pCMV-NLS-hTtAgo-NLS-3xFLAG; pLenti-CAG-Puro-DEST;
                     pMLM3705; pMLM3705 annotation mess"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pCMV-T7-Cas9 (MLM3613), pCMV-T7-Cas9
                     delta NcoI"
                     /Transferred_Similarity="100.00%"
                     /modified_by="ben"
                     /label="CAG_enhancer"
ORIGIN      
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gcctagaaag
      421 atgttcagct gcgatttcga aaccacaacc aagctggacg actgcagagt gtgggcttac
      481 ggctacatgg aaatcggcaa cctggataac tacaagattg gaaacagcct ggatgagttc
      541 atgcagtggg tgatggaaat ccaggccgac ctgtacttcc acaacctgaa gtttgacggc
      601 gccttcatag tgaactggct ggaaagacac ggcttcaagt ggagcaacga gggcctgccc
      661 aacacctaca ataccatcat ttctaagatg ggccagtggt acatgatcga tatctgtttc
      721 ggctataagg gcaagcggaa gctgcacacc gtgatctacg acagcctgaa gaagctgcca
      781 ttccctgtca aaaagatcgc caaagatttt cagctgcctc tgctgaaagg cgatatcgac
      841 taccacaagg agcggcctgt cggccacgaa atcacaccag aggaatatga atacatcaag
      901 aatgacatcg agatcatcgc ccgggccctg gacatccagt tcaagcaagg cctggataga
      961 atgaccgctg gctctgattc tctgaagggg ttcaaagata tcctgagcac caagaagttc
     1021 aacaaggtgt tccccaagtt aagcctgcct atggacaagg aaatccgcta cgcctatagg
     1081 ggcggcttta catggctgaa cgacaaatac aaggaaaagg agattggaga aggcatggtg
     1141 ttcgatgtga actctctgta tccttcccag atgtacagca gacctctgcc ttacggcgcc
     1201 cccatcgtgt tccagggcaa gtacgagaag gacgagcagt accccctgta catccaaaga
     1261 atcagattcg agttcgagct gaaagagggc tatatcccca ccatccagat caaaaaaaac
     1321 cccttcttca agggcaatga gtacctgaag aactccggcg gagagcctgt tgaactgtac
     1381 ctgaccaacg tggatctgga actgatccag gagcactacg agctgtacaa cgtggagtac
     1441 attgacggct ttaaatttag agaaaaaacc ggcttgttta aggacttcat cgacaagtgg
     1501 acctacgtga agacacacga ggaaggagca aagaaacagc tggccaagct gatgctgaac
     1561 agcctgtacg gcaagtttgc cagcaatcct gacgtgaccg gcaaagttcc atacctcaaa
     1621 gaggacggat ctctgggctt ccgggtgggc gacgaagaat acaaggaccc cgtgtacacc
     1681 cctatgggag tgtttatcac agcctgggcc agattcacca ccatcaccgc cgcccaggct
     1741 tgttacgacc ggatcatcta ctgcgacaca gacagcatcc acctgacagg caccgaggtg
     1801 cctgagatca tcaaggacat cgtggaccct aagaagctgg gctactgggc tcatgagtcc
     1861 accttcaaaa gagccaagta cctgagacag aagacctaca tccaggacat ctacatgaag
     1921 gaagttgacg gaaagctggt ggagtgcagc cctgatgagg ccaccacaac caagttctcc
     1981 gtgaagtgcg ccggcatgac agataccatc aaaaagaagg tgacattcga caacttcaag
     2041 gtgggcttca gcagcaaggg aaagcctaaa ccagtgcaag tgaacggcgg agtggtgctg
     2101 gtagacagcg tgttcacaat caagtccggc ggaagctctg gtggcagcaa gcggaccgcc
     2161 gacggctctg aattcgagag ccctaagaag aaaagaaagg tgtgaccggt catcatcacc
     2221 atcaccattg agtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     2281 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     2341 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     2401 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     2461 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tcgataccgt
     2521 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     2581 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctagggtg
     2641 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     2701 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     2761 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     2821 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     2881 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     2941 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     3001 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     3061 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     3121 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     3181 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     3241 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     3301 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     3361 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     3421 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     3481 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     3541 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     3601 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     3661 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     3721 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     3781 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     3841 caatgatacc gcgcgaacca cgctcaccgg ctccagattt atcagcaata aaccagccag
     3901 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     3961 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     4021 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     4081 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     4141 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     4201 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     4261 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     4321 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     4381 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     4441 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     4501 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     4561 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     4621 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     4681 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcgatct cccgatcccc
     4741 tagggtcgac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
     4801 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
     4861 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
     4921 gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat
     4981 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     5041 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     5101 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     5161 ggtaaactgc ccacttggca gtacatcaag tgtatc
//
