LOCUS       pCMV-T7-Phi29(+exo)-CC(N5)_(LM2761)        5322 bp    DNA     circular SYN 24-NOV-2023
DEFINITION  JDS246.
ACCESSION   urn.local...116-h0aqq85
KEYWORDS    .
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     enhancer        132..356
                     /created_by="lha16"
                     /modified_by="lha16"
                     /label="CMV_promoter"
     promoter        377..396
                     /X-Transferred_From="pCRII-TOPO"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pCMV-T7-Cas9 (MLM3613), pCMV-T7-Cas9
                     delta NcoI"
                     /Transferred_Similarity="100.00%"
                     /modified_by="lha16"
                     /label="T7 promoter"
     CDS             412..2133
                     /created_by="KleinstiverLab8"
                     /Color="#ee0077"
                     /modified_by="lha16"
                     /label="Phi29(+exo)"
     CDS             2134..2157
                     /created_by="lha16"
                     /Color="#ff7400"
                     /modified_by="lha16"
                     /label="GS linker"
     CDS             2158..2211
                     /Color="#6bba70"
                     /created_by="lha16"
                     /label="BPNLS"
     source          <2212..>2328
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          <2212..>2235
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             <2212..2235
                     /codon_start=1
                     /created_by="Kleinstiver Lab 1"
                     /Color="#ff7400"
                     /modified_by="lha16"
                     /label="GS linker"
     CDS             2236..2328
                     /created_by="bpk6"
                     /Color="#ff77ff"
                     /modified_by="lha16"
                     /label="CC(N5)"
     CDS             2329..2331
                     /Color="#000000"
                     /created_by="lha16"
                     /label
     terminator      2380..2607
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIK
                     KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
                     ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH
                     MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSK
                     SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD
                     NLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTL
                     LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVK
                     LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
                     YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL
                     PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKE
                     DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT
                     LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF
                     LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT
                     VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKE
                     HPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKV
                     LTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA
                     GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG
                     KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
                     MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV
                     AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLF
                     ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH
                     KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA
                     PAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDGSPKKKRKVS
                     SDYKDHDGDYKDHDIDYKDDDDKAAG*"
                     /label="bGH_PA_terminator"
     terminator      2866..2997
                     /SerialCloner_Color="&hF08080"
                     /SerialCloner_Show="False"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""SV40_PA_terminator"" -
                     translation=""MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     -
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     -
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     - GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLST \"
                     /X-Transferred_From="3.
                     pCAG-nls-Csy4-FokI-T2A-dCas9-recoded (C-terminal fusion) -
                     linker variants; 6.pCAG-nls-FokI-Csy4-T2A-Cas9-recoded
                     (N-terminal fusion) -linker variants; JDS246;
                     VC-(VC780+Csy4-Cas9)_pLKO-TRC001;
                     pCAG-Csy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK376); pCAG-Csy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64
                     recoded (BPK376frameshift);
                     pCAG-Csy4-P2A-dCas9-NLS-FLAG-VP64 recoded;
                     pCAG-Csy4-T2A-Cas9-VP64 recoded (BPK1);
                     pCAG-Csy4-T2A-Cas9-VP64 recoded (BPK2a);
                     pCAG-Csy4-T2A-Cas9-recoded (SQT834);
                     pCAG-Csy4-T2A-FokI-dCas9-recoded-nls (JAF1484);
                     pCAG-Csy4-T2A-KRAB-dCas9-recoded-nlsFLAG (NW9);
                     pCAG-Csy4-T2A-NLS-FLAG-NLS-VP64-dCas9-recoded (making
                     p125); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-KRAB recoded
                     (BPK353); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-VP64 recoded
                     (BPK326); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-VP64 recoded NO
                     CTERM FLAG;
                     pCAG-Csy4-T2A-VP64-NLS/FLAG/NLS-dCas9-recoded-nlsFLAG
                     (BPK328); pCAG-Csy4-T2A-VP64-dCas9 linker variant BbsI
                     cassette; pCAG-Csy4-T2A-VP64-dCas9-recoded-nlsFLAG
                     (BPK285); pCAG-Csy4-T2A-VP64-dCas9-recoded-nlsFLAG
                     (BPK286); pCAG-Csy4-T2A-dCas9-KRAB recoded (NW8);
                     pCAG-Csy4-T2A-dCas9-VP64 recoded (BPK3);
                     pCAG-Csy4-T2A-dCas9-VP64 recoded NO FLAG (BPK373 or 374?);
                     pCAG-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64;
                     pCAG-NLS-FLAG-NLS-dCas9-VP64 recoded;
                     pCAG-NLS-FLAG-VP64-dCas9-recoded;
                     pCAG-NLS-VP64-dCas9-recoded; pCAG-NLS-dCas9-NLS-3xFLAG
                     recoded; pCAG-NLS-dCas9-VP64 recoded; pCAG-NLS-dCas9-VP64
                     recoded CopyXXXXX;
                     pCAG-NLS-hCsy4-P2A-NLS-FLAG-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-NLS-hTtAgo (BPK633); pCAG-NLS-hTtAgo-NLS-3xFLAG
                     (BPK573); pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64;
                     pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64 D478A/D546A;
                     pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64 D478N/D546N;
                     pCAG-VP64-NLS-FLAG-NLS-dCas9-recoded-NLS-FLAG (BPK329);
                     pCAG-VP64-NLS-hTtAgo; pCAG-VP64-dCas9-recod linker variant
                     BbsI cassette; pCAG-VP64-dCas9-recoded GGGGSx5 linker;
                     pCAG-VP64-dCas9-recoded-NLS-FLAG (BPK278);
                     pCAG-VP64-hCsy4-NLS-P2A-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-dCas9-VP64;
                     pCAG-hCsy4-NLS-P2A-NLS-FLAG-NLS-hdCas9-NLS-FLAG-VP64
                     (BPK553);
                     pCAG-hCsy4-NLS-VP64-P2A-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-3xFLAG-KRAB
                     (BPK550); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64
                     delta175-307 (BPK544);
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded;
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK447);
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64-GG recoded
                     (BPK531); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-KRAB
                     (BPK454); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-VP64
                     recoded (BPK453); pCAG-hCsy4-P2A-NLS-dCas9-NLS-FLAG-VP64
                     recoded (BPK546); pCAG-hCsy4-P2A-NLS-dCas9-NLS-KRAB
                     (BPK548); pCAG-hCsy4-P2A-dCas9-NLS-FLAG-VP64 recoded
                     (BPK422); pCAG-hCsy4-P2A-dCas9-NLS-KRAB (BPK439);
                     pCAG-hCsy4-P2A-dCas9-NLS-VP64 recoded (BPK450);
                     pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK428); pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-KRAB
                     (BPK444); pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-VP64
                     recoded (BPK437); pCAG-hCsy4-T2A-dCas9-NLS-FLAG-VP64
                     recoded (BPK426); pCAG-hCsy4-T2A-dCas9-NLS-KRAB (BPK442);
                     pCAG-hCsy4-T2A-dCas9-NLS-VP64 recoded (BPK435);
                     pCAG-hCsy4-T2A-nls-hFokI-dhCas9-nls-3XFLAG (SQT1601);
                     pCAG-hTtAgo; pCAG-hTtAgo-NLS; pCAG-hTtAgo-NLS-3xFLAG;
                     pCAG-nls-FokI-Csy4-T2A-dCas9-recoded (N-terminal fusion
                     CK1891); pCMV-NLS-hTtAgo-NLS-3xFLAG; pCW-Cas9 (modified);
                     pCW-Cas9 Activator; pCW-Cas9 N-F-N Activator; pCW-Cas9
                     N-F-N Activator-GG; pCW-EFS-Puro-P2A-NFN-dCas9-VP64;
                     pCW-NLS-dCas9-VP64 EFS-Puro-T2A-rTta3-WPRE;
                     pEF1a-Csy4-T2A-NLS-FLAG-NLS-dCas9-VP64 recoded;
                     pEF1a-Csy4-T2A-dCas9-VP64 recoded;
                     pEF1a-Csy4-T2A-dCas9-VP64 recoded TRUNC;
                     pEF1a-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded;
                     pEFS-Csy4-T2A-dCas9-VP64 recoded;
                     pEFS-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK533); pLV-CMVEI.EGFPDS-iNpLTRSEBBA6_0693;
                     pLenti-TREminCMV-NLS-3xFLAG-NLS-dCas9-VP64
                     EFS-Puro-T2A-rTta3-WPRE; pLenti-TREminCMV-NLS-dCas9-VP64
                     EFS-Puro-T2A-rTta3-WPRE; pMLM3705; pMLM3705 annotation
                     mess; pWPXLd (modified); pcDNA5/FRT;
                     pcDNA5/FRT-tdtomato-AAVS1 site 1;
                     pcDNA5/FRT-tdtomato-AAVS1 site 1 in wrong way;
                     pcDNA5/FRT-tdtomato-AAVS1 site 2"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pCMV-T7-Cas9 (MLM3613), pCMV-T7-Cas9
                     delta NcoI"
                     /Transferred_Similarity="100.00%"
                     /modified_by="KleinstiverLab8"
                     /label="SV40_PA_terminator"
     misc_feature    complement(3065..3684)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /X-Transferred_From="pBR322 origin"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pCAG-hCas9-nls-3XFLAG (SQT817),
                     pCAG-hCas9-nls-3XFLAG delta NcoI (BPK742), pCMV-T7-Cas9
                     (MLM3613), pCMV-T7-Cas9 delta NcoI"
                     /Transferred_Similarity="100.00%"
                     /label="pBR322 origin"
                     /note="Geneious type: origin of replication"
     CDS             complement(3839..4699)
                     /gene="Ampicillin"
                     /Original_Translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVL
                     GNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKV
                     DDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADL
                     RLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAK
                     AILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLS
                     KDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRY
                     DEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKM
                     DGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIE
                     KILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFD
                     KNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNR
                     KVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDI
                     LEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDK
                     QSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSP
                     AIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIK
                     ELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFL
                     KDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAER
                     GGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLV
                     SDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKM
                     IAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRD
                     FATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSP
                     TVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLI
                     IKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNE
                     QKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIH
                     LFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDG
                     SPKKKRKVSSDYKDHDGDYKDHDIDYKDDDDKAAG*"
                     /Color="#777777"
                     /modified_by="lha16"
                     /label="Ampicillin"
     promoter        complement(4700..4804)
                     /X-Transferred_From="JDS246; pCMV-NLS-hTtAgo-NLS-3xFLAG;
                     pLV-CMVEI.EGFPDS-iNpLTRSEBBA6_0693; pLVX-TetOne-puro;
                     pMLM3705; pMLM3705 annotation mess"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pCMV-T7-Cas9 (MLM3613), pCMV-T7-Cas9
                     delta NcoI"
                     /Transferred_Similarity="100.00%"
                     /label="AmpR promoter"
     promoter        5088..131
                     /SerialCloner_Color="&h03C000"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /X-Transferred_From="3.
                     pCAG-nls-Csy4-FokI-T2A-dCas9-recoded (C-terminal fusion) -
                     linker variants; 6.pCAG-nls-FokI-Csy4-T2A-Cas9-recoded
                     (N-terminal fusion) -linker variants; JDS246;
                     pCAG-Csy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK376); pCAG-Csy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64
                     recoded (BPK376frameshift);
                     pCAG-Csy4-P2A-dCas9-NLS-FLAG-VP64 recoded;
                     pCAG-Csy4-T2A-Cas9-VP64 recoded (BPK1);
                     pCAG-Csy4-T2A-Cas9-VP64 recoded (BPK2a);
                     pCAG-Csy4-T2A-Cas9-recoded (SQT834);
                     pCAG-Csy4-T2A-FokI-dCas9-recoded-nls (JAF1484);
                     pCAG-Csy4-T2A-KRAB-dCas9-recoded-nlsFLAG (NW9);
                     pCAG-Csy4-T2A-NLS-FLAG-NLS-VP64-dCas9-recoded (making
                     p125); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-KRAB recoded
                     (BPK353); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-VP64 recoded
                     (BPK326); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-VP64 recoded NO
                     CTERM FLAG;
                     pCAG-Csy4-T2A-VP64-NLS/FLAG/NLS-dCas9-recoded-nlsFLAG
                     (BPK328); pCAG-Csy4-T2A-VP64-dCas9 linker variant BbsI
                     cassette; pCAG-Csy4-T2A-VP64-dCas9-recoded-nlsFLAG
                     (BPK285); pCAG-Csy4-T2A-VP64-dCas9-recoded-nlsFLAG
                     (BPK286); pCAG-Csy4-T2A-dCas9-KRAB recoded (NW8);
                     pCAG-Csy4-T2A-dCas9-VP64 recoded (BPK3);
                     pCAG-Csy4-T2A-dCas9-VP64 recoded NO FLAG (BPK373 or 374?);
                     pCAG-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64;
                     pCAG-NLS-FLAG-NLS-dCas9-VP64 recoded;
                     pCAG-NLS-FLAG-VP64-dCas9-recoded;
                     pCAG-NLS-VP64-dCas9-recoded; pCAG-NLS-dCas9-NLS-3xFLAG
                     recoded; pCAG-NLS-dCas9-VP64 recoded; pCAG-NLS-dCas9-VP64
                     recoded CopyXXXXX;
                     pCAG-NLS-hCsy4-P2A-NLS-FLAG-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-NLS-hTtAgo (BPK633); pCAG-NLS-hTtAgo-NLS-3xFLAG
                     (BPK573); pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64;
                     pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64 D478A/D546A;
                     pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64 D478N/D546N;
                     pCAG-VP64-NLS-FLAG-NLS-dCas9-recoded-NLS-FLAG (BPK329);
                     pCAG-VP64-NLS-hTtAgo; pCAG-VP64-dCas9-recod linker variant
                     BbsI cassette; pCAG-VP64-dCas9-recoded GGGGSx5 linker;
                     pCAG-VP64-dCas9-recoded-NLS-FLAG (BPK278);
                     pCAG-VP64-hCsy4-NLS-P2A-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-dCas9-VP64;
                     pCAG-hCsy4-NLS-P2A-NLS-FLAG-NLS-hdCas9-NLS-FLAG-VP64
                     (BPK553);
                     pCAG-hCsy4-NLS-VP64-P2A-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-3xFLAG-KRAB
                     (BPK550); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64
                     delta175-307 (BPK544);
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded;
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK447);
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64-GG recoded
                     (BPK531); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-KRAB
                     (BPK454); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-VP64
                     recoded (BPK453); pCAG-hCsy4-P2A-NLS-dCas9-NLS-FLAG-VP64
                     recoded (BPK546); pCAG-hCsy4-P2A-NLS-dCas9-NLS-KRAB
                     (BPK548); pCAG-hCsy4-P2A-dCas9-NLS-FLAG-VP64 recoded
                     (BPK422); pCAG-hCsy4-P2A-dCas9-NLS-KRAB (BPK439);
                     pCAG-hCsy4-P2A-dCas9-NLS-VP64 recoded (BPK450);
                     pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK428); pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-KRAB
                     (BPK444); pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-VP64
                     recoded (BPK437); pCAG-hCsy4-T2A-dCas9-NLS-FLAG-VP64
                     recoded (BPK426); pCAG-hCsy4-T2A-dCas9-NLS-KRAB (BPK442);
                     pCAG-hCsy4-T2A-dCas9-NLS-VP64 recoded (BPK435);
                     pCAG-hCsy4-T2A-nls-hFokI-dhCas9-nls-3XFLAG (SQT1601);
                     pCAG-hTtAgo; pCAG-hTtAgo-NLS; pCAG-hTtAgo-NLS-3xFLAG;
                     pCAG-nls-FokI-Csy4-T2A-dCas9-recoded (N-terminal fusion
                     CK1891); pCMV-NLS-hTtAgo-NLS-3xFLAG; pLenti-CAG-Puro-DEST;
                     pMLM3705; pMLM3705 annotation mess"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pCMV-T7-Cas9 (MLM3613), pCMV-T7-Cas9
                     delta NcoI"
                     /Transferred_Similarity="100.00%"
                     /modified_by="lha16"
                     /label="CMV Enhancer"
     promoter        complement(5137..5165)
                     /SerialCloner_Color="&hF08080"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""AmpR_promoter"" -
                     translation=""MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     -
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     -
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     - DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWM \"
                     /X-Transferred_From="3.
                     pCAG-nls-Csy4-FokI-T2A-dCas9-recoded (C-terminal fusion) -
                     linker variants; 6.pCAG-nls-FokI-Csy4-T2A-Cas9-recoded
                     (N-terminal fusion) -linker variants; JDS246;
                     pCAG-Csy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK376); pCAG-Csy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64
                     recoded (BPK376frameshift);
                     pCAG-Csy4-P2A-dCas9-NLS-FLAG-VP64 recoded;
                     pCAG-Csy4-T2A-Cas9-VP64 recoded (BPK1);
                     pCAG-Csy4-T2A-Cas9-VP64 recoded (BPK2a);
                     pCAG-Csy4-T2A-Cas9-recoded (SQT834);
                     pCAG-Csy4-T2A-FokI-dCas9-recoded-nls (JAF1484);
                     pCAG-Csy4-T2A-KRAB-dCas9-recoded-nlsFLAG (NW9);
                     pCAG-Csy4-T2A-NLS-FLAG-NLS-VP64-dCas9-recoded (making
                     p125); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-KRAB recoded
                     (BPK353); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-VP64 recoded
                     (BPK326); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-VP64 recoded NO
                     CTERM FLAG;
                     pCAG-Csy4-T2A-VP64-NLS/FLAG/NLS-dCas9-recoded-nlsFLAG
                     (BPK328); pCAG-Csy4-T2A-VP64-dCas9 linker variant BbsI
                     cassette; pCAG-Csy4-T2A-VP64-dCas9-recoded-nlsFLAG
                     (BPK285); pCAG-Csy4-T2A-VP64-dCas9-recoded-nlsFLAG
                     (BPK286); pCAG-Csy4-T2A-dCas9-KRAB recoded (NW8);
                     pCAG-Csy4-T2A-dCas9-VP64 recoded (BPK3);
                     pCAG-Csy4-T2A-dCas9-VP64 recoded NO FLAG (BPK373 or 374?);
                     pCAG-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64;
                     pCAG-NLS-FLAG-NLS-dCas9-VP64 recoded;
                     pCAG-NLS-FLAG-VP64-dCas9-recoded;
                     pCAG-NLS-VP64-dCas9-recoded; pCAG-NLS-dCas9-NLS-3xFLAG
                     recoded; pCAG-NLS-dCas9-VP64 recoded; pCAG-NLS-dCas9-VP64
                     recoded CopyXXXXX;
                     pCAG-NLS-hCsy4-P2A-NLS-FLAG-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-NLS-hTtAgo (BPK633); pCAG-NLS-hTtAgo-NLS-3xFLAG
                     (BPK573); pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64;
                     pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64 D478A/D546A;
                     pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64 D478N/D546N;
                     pCAG-VP64-NLS-FLAG-NLS-dCas9-recoded-NLS-FLAG (BPK329);
                     pCAG-VP64-NLS-hTtAgo; pCAG-VP64-dCas9-recod linker variant
                     BbsI cassette; pCAG-VP64-dCas9-recoded GGGGSx5 linker;
                     pCAG-VP64-dCas9-recoded-NLS-FLAG (BPK278);
                     pCAG-VP64-hCsy4-NLS-P2A-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-dCas9-VP64;
                     pCAG-hCsy4-NLS-P2A-NLS-FLAG-NLS-hdCas9-NLS-FLAG-VP64
                     (BPK553);
                     pCAG-hCsy4-NLS-VP64-P2A-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-3xFLAG-KRAB
                     (BPK550); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64
                     delta175-307 (BPK544);
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded;
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK447);
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64-GG recoded
                     (BPK531); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-KRAB
                     (BPK454); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-VP64
                     recoded (BPK453); pCAG-hCsy4-P2A-NLS-dCas9-NLS-FLAG-VP64
                     recoded (BPK546); pCAG-hCsy4-P2A-NLS-dCas9-NLS-KRAB
                     (BPK548); pCAG-hCsy4-P2A-dCas9-NLS-FLAG-VP64 recoded
                     (BPK422); pCAG-hCsy4-P2A-dCas9-NLS-KRAB (BPK439);
                     pCAG-hCsy4-P2A-dCas9-NLS-VP64 recoded (BPK450);
                     pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK428); pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-KRAB
                     (BPK444); pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-VP64
                     recoded (BPK437); pCAG-hCsy4-T2A-dCas9-NLS-FLAG-VP64
                     recoded (BPK426); pCAG-hCsy4-T2A-dCas9-NLS-KRAB (BPK442);
                     pCAG-hCsy4-T2A-dCas9-NLS-VP64 recoded (BPK435);
                     pCAG-hCsy4-T2A-nls-hFokI-dhCas9-nls-3XFLAG (SQT1601);
                     pCAG-hTtAgo; pCAG-hTtAgo-NLS; pCAG-hTtAgo-NLS-3xFLAG;
                     pCAG-nls-FokI-Csy4-T2A-dCas9-recoded (N-terminal fusion
                     CK1891); pCMV-NLS-hTtAgo-NLS-3xFLAG; pLenti-CAG-Puro-DEST;
                     pMLM3705; pMLM3705 annotation mess; pcDNA5/FRT"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pCMV-T7-Cas9 (MLM3613), pCMV-T7-Cas9
                     delta NcoI"
                     /Transferred_Similarity="100.00%"
                     /label="AmpR_promoter"
     promoter        5154..119
                     /SerialCloner_Color="&hDC143C"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""CAG_enhancer"" - 84..371 \"
                     /X-Transferred_From="3.
                     pCAG-nls-Csy4-FokI-T2A-dCas9-recoded (C-terminal fusion) -
                     linker variants; 6.pCAG-nls-FokI-Csy4-T2A-Cas9-recoded
                     (N-terminal fusion) -linker variants; JDS246;
                     pCAG-AttB-Csy4-VEGFA1-3 (BPK318); pCAG-CFP;
                     pCAG-Csy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK376); pCAG-Csy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64
                     recoded (BPK376frameshift);
                     pCAG-Csy4-P2A-dCas9-NLS-FLAG-VP64 recoded;
                     pCAG-Csy4-T2A-Cas9-VP64 recoded (BPK1);
                     pCAG-Csy4-T2A-Cas9-VP64 recoded (BPK2a);
                     pCAG-Csy4-T2A-Cas9-recoded (SQT834);
                     pCAG-Csy4-T2A-FokI-dCas9-recoded-nls (JAF1484);
                     pCAG-Csy4-T2A-KRAB-dCas9-recoded-nlsFLAG (NW9);
                     pCAG-Csy4-T2A-NLS-FLAG-NLS-VP64-dCas9-recoded (making
                     p125); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-KRAB recoded
                     (BPK353); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-VP64 recoded
                     (BPK326); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-VP64 recoded NO
                     CTERM FLAG;
                     pCAG-Csy4-T2A-VP64-NLS/FLAG/NLS-dCas9-recoded-nlsFLAG
                     (BPK328); pCAG-Csy4-T2A-VP64-dCas9 linker variant BbsI
                     cassette; pCAG-Csy4-T2A-VP64-dCas9-recoded-nlsFLAG
                     (BPK285); pCAG-Csy4-T2A-VP64-dCas9-recoded-nlsFLAG
                     (BPK286); pCAG-Csy4-T2A-dCas9-KRAB recoded (NW8);
                     pCAG-Csy4-T2A-dCas9-VP64 recoded (BPK3);
                     pCAG-Csy4-T2A-dCas9-VP64 recoded NO FLAG (BPK373 or 374?);
                     pCAG-Csy4site2-gRNA-Csy4site2 (SQT1468) + 1 site;
                     pCAG-Csy4site2-gRNA-Csy4site2 (SQT1468) + 4 sites;
                     pCAG-Csy4site2-gRNA-Csy4site2 BsaI;
                     pCAG-Csy4site2-gRNA-Csy4site2 BsaI cassette; pCAG-DEST
                     (BPK88); pCAG-DEST V2;
                     pCAG-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64;
                     pCAG-NLS-FLAG-NLS-dCas9-VP64 recoded;
                     pCAG-NLS-FLAG-VP64-dCas9-recoded;
                     pCAG-NLS-VP64-dCas9-recoded; pCAG-NLS-dCas9-NLS-3xFLAG
                     recoded; pCAG-NLS-dCas9-VP64 recoded; pCAG-NLS-dCas9-VP64
                     recoded CopyXXXXX;
                     pCAG-NLS-hCsy4-P2A-NLS-FLAG-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-NLS-hTtAgo (BPK633); pCAG-NLS-hTtAgo-NLS-3xFLAG
                     (BPK573); pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64;
                     pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64 D478A/D546A;
                     pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64 D478N/D546N;
                     pCAG-VP64-NLS-FLAG-NLS-dCas9-recoded-NLS-FLAG (BPK329);
                     pCAG-VP64-NLS-hTtAgo; pCAG-VP64-dCas9-recod linker variant
                     BbsI cassette; pCAG-VP64-dCas9-recoded GGGGSx5 linker;
                     pCAG-VP64-dCas9-recoded-NLS-FLAG (BPK278);
                     pCAG-VP64-hCsy4-NLS-P2A-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-dCas9-VP64;
                     pCAG-hCsy4-NLS-P2A-NLS-FLAG-NLS-hdCas9-NLS-FLAG-VP64
                     (BPK553);
                     pCAG-hCsy4-NLS-VP64-P2A-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-3xFLAG-KRAB
                     (BPK550); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64
                     delta175-307 (BPK544);
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded;
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK447);
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64-GG recoded
                     (BPK531); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-KRAB
                     (BPK454); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-VP64
                     recoded (BPK453); pCAG-hCsy4-P2A-NLS-dCas9-NLS-FLAG-VP64
                     recoded (BPK546); pCAG-hCsy4-P2A-NLS-dCas9-NLS-KRAB
                     (BPK548); pCAG-hCsy4-P2A-dCas9-NLS-FLAG-VP64 recoded
                     (BPK422); pCAG-hCsy4-P2A-dCas9-NLS-KRAB (BPK439);
                     pCAG-hCsy4-P2A-dCas9-NLS-VP64 recoded (BPK450);
                     pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded
                     (BPK428); pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-KRAB
                     (BPK444); pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-VP64
                     recoded (BPK437); pCAG-hCsy4-T2A-dCas9-NLS-FLAG-VP64
                     recoded (BPK426); pCAG-hCsy4-T2A-dCas9-NLS-KRAB (BPK442);
                     pCAG-hCsy4-T2A-dCas9-NLS-VP64 recoded (BPK435);
                     pCAG-hCsy4-T2A-nls-hFokI-dhCas9-nls-3XFLAG (SQT1601);
                     pCAG-hTtAgo; pCAG-hTtAgo-NLS; pCAG-hTtAgo-NLS-3xFLAG;
                     pCAG-nls-FokI-Csy4-T2A-dCas9-recoded (N-terminal fusion
                     CK1891); pCMV-NLS-hTtAgo-NLS-3xFLAG; pLenti-CAG-Puro-DEST;
                     pMLM3705; pMLM3705 annotation mess"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pCMV-T7-Cas9 (MLM3613), pCMV-T7-Cas9
                     delta NcoI"
                     /Transferred_Similarity="100.00%"
                     /modified_by="ben"
                     /label="CAG_enhancer"
ORIGIN      
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaagcacatg
      421 cctagaaaga tgtacagctg cgacttcgag acaacaacca aggtcgagga ctgcagagtg
      481 tgggcttacg gatatatgaa tatcgaggac cactctgagt acaagatcgg aaatagcctg
      541 gatgagttta tggcctgggt cctgaaagtg caggccgacc tgtacttcca caacctgaaa
      601 ttcgacggcg ccttcatcat taactggctg gaacggaacg gctttaagtg gtccgccgat
      661 ggactgccaa acacctacaa caccatcatc agcagaatgg gccagtggta catgatcgac
      721 atctgcctgg gctacaaggg caaaagaaag atccataccg tgatctacga cagcctgaag
      781 aaactgcctt tccccgtgaa gaagatcgct aaagacttca agctgacagt gctgaagggc
      841 gacattgatt accacaagga aagacctgtg ggatacaaga tcacacctga ggaatatgcc
      901 tacatcaaga acgacatcca gatcatcgcc gaggccctgc tgatccagtt caagcagggc
      961 ctggaccgga tgaccgctgg cagcgacagc ctcaaaggct tcaaggacat tatcaccact
     1021 aagaagttca aaaaggtgtt cccaacactg tctcttggac tggacaagga agtgcggtac
     1081 gcctatagag gcggctttac ctggctgaac gacagattca aggaaaagga aatcggcgag
     1141 ggcatggtgt ttgacgtgaa ctccctctac cctgcccaga tgtacagccg gctgctgccc
     1201 tatggcgagc ctatcgtgtt tgagggcaag tacgtgtggg atgaagatta ccccctgcac
     1261 atccagcaca tccggtgcga gttcgagctg aaggagggct acatcccaac aatccaaatc
     1321 aaacggagca gattctacaa gggcaacgag tacctgaaaa gcagcggcgg cgaaatcgcc
     1381 gatctgtggc tgagcaatgt ggacctggaa ctgatgaagg aacactacga cctgtacaac
     1441 gtggaataca tcagcggcct gaagtttaag gccacaaccg gcctgttcaa agacttcatc
     1501 gataagtgga cctacatcaa aacaaccagc gagggcgcta tcaagcagct ggccaagctg
     1561 atgctgaatt ctctgtacgg caagttcgcc agcaaccctg atgtcacagg caaggtgccc
     1621 tacctgaagg aaaacggcgc cctgggcttc agactgggcg aggaagaaac caaggaccct
     1681 gtgtacaccc ctatgggtgt tttcatcacc gcctgggcca gatacaccac catcaccgcc
     1741 gctcaggctt gttacgatag aatcatctat tgtgataccg actctatcca cctgaccggg
     1801 accgagatcc ccgacgtgat caaggatatt gtggacccca agaaactggg ctactgggcc
     1861 cacgagtcca ccttcaagcg ggccaagtac ctgagacaga agacctacat ccaggacatc
     1921 tacatgaaag aagtggacgg caagctcgtg gaaggcagcc ccgacgacta cacagacatc
     1981 aagttcagcg tgaagtgcgc cggcatgacc gataagatca aaaaggaggt gacatttgag
     2041 aacttcaagg tgggattcag ccgcaagatg aaacctaagc ctgttcaagt gcctggcggt
     2101 gttgtgctgg tggatgacac cttcacaatc aagtccggcg gaagctctgg tggcagcaag
     2161 cggaccgccg acggctctga attcgagagc cctaagaaga aaagaaaggt gagcggtggg
     2221 agcagcggcg ggtcaggcga gatcgccgca ctggaagcca agatcgctgc cctgaaggca
     2281 aagaacgccg ctctgaaggc cgagatcgcc gccctggaag ccggatactg accggtcatc
     2341 atcaccatca ccattgagtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     2401 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     2461 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     2521 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     2581 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcga
     2641 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     2701 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     2761 agggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     2821 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     2881 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     2941 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     3001 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     3061 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     3121 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     3181 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     3241 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     3301 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     3361 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     3421 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     3481 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     3541 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     3601 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     3661 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     3721 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     3781 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     3841 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     3901 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     3961 gtgctgcaat gataccgcgc gaaccacgct caccggctcc agatttatca gcaataaacc
     4021 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     4081 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     4141 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     4201 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     4261 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     4321 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     4381 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     4441 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     4501 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     4561 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     4621 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     4681 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     4741 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     4801 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat cgatctcccg
     4861 atcccctagg gtcgactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
     4921 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
     4981 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
     5041 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
     5101 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     5161 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     5221 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     5281 atttacggta aactgcccac ttggcagtac atcaagtgta tc
//
