LOCUS       pUC19-U6-[BsmBI_entry]-term (MNW320) - pegRNA entry vector        2204 bp    DNA     circular     21-NOV-2023
DEFINITION  .
ACCESSION   urn.local...b-4oqvoi1
VERSION     urn.local...b-4oqvoi1
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     Serial Cloner Genbank Format SerialCloner_Type=DNA                 
            SerialCloner_Comments=[DEFINITION]: - [LOCUS]:                     
            pUC19-U6-+103gRNA.seq SerialCloner_Ends=0,0,,0,.
FEATURES             Location/Qualifiers
     regulatory      13..330
                     /note="Geneious type: promoter"
                     /standard_name="U6 promoter"
     misc_binding    331..351
     misc_feature    complement(332..337)
                     /note="Geneious type: misc_marker"
                     /standard_name="BsmBI"
     misc_feature    345..350
                     /note="Geneious type: misc_marker"
                     /standard_name="BsmBI"
     misc_RNA        352..358
                     /note="Geneious type: terminator"
                     /standard_name="U6 terminator"
     rep_origin      complement(396..1069)
     CDS             complement(1214..2074)
                     /standard_name="ampR"
     misc_feature    complement(1354..1359)
                     /note="Geneious type: misc_marker"
                     /standard_name="BsaI"
     regulatory      complement(2116..2144)
                     /note="Geneious type: promoter"
                     /standard_name="amp promoter"
ORIGIN      
        1 gacgtcgcta gctgtacaaa aaagcaggct ttaaaggaac caattcagtc gactggatcc
       61 ggtaccaagg tcgggcagga agagggccta tttcccatga ttccttcata tttgcatata
      121 cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta
      181 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
      241 tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct
      301 ttatatatct tgtggaaagg acgaaacacc ggagacgatt aatgcgtctc ctttttttaa
      361 gcttgggccg ctcgaggtac ctctctacat atgacatgtg agcaaaaggc cagcaaaagg
      421 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      481 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      541 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      601 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
      661 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      721 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      781 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      841 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
      901 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      961 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     1021 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     1081 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     1141 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     1201 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     1261 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     1321 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1381 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1441 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1501 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1561 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1621 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1681 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1741 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1801 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1861 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1921 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1981 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     2041 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     2101 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     2161 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acct
//
