LOCUS       pcDNA3.1-CRISPR-Puro-sgRPL3-56    10870 bp      DNA     circular   2023/9/6
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    6447..919
                     /label=pcDNA3.1 (-) backbone
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=pcDNA3.1 (-) backbone \ 
     misc_feature    927..5776
                     /label=CRISPR/csa9
                     /SerialCloner_Color=&h8080FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=containing U6 promoter and sgRNA  cloned from lentiCRISPRv2 \ 
     misc_feature    968..1200
                     /label=hU6p
                     /SerialCloner_Color=&hFF8080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=human U6 promoter \ 
     CDS             1602..6440
                     /label=Cas9 ORF
                     /SerialCloner_Color=&h0080FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=Cas9 ORF from lentiCRISPRv2 \ 
     misc_feature    5754..5777
                     /label=Flag
                     /SerialCloner_Color=&hFF80FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=Flag \ 
     sig_peptide     5706..5753
                     /label=NLS
                     /SerialCloner_Color=&h8000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=Xenopus laevis Nucleoplasmin NLS from lentiCRISPRv2 \ 
     misc_feature    5787..5843
                     /label=Eukaryotic ribosomes fail to insert a peptide bond _P2A
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=Eukaryotic ribosomes fail to insert a peptide bond _P2A from lentiCRISPRv2 \ 
     misc_feature    5784..6440
                     /label=PuroR
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=Puromycin resistance gene from lentiCRISPRv2 \ 
     misc_feature    1209..1228
                     /label=RPL3-sgR56
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=targeting RPL3 3' region in human genome (GRCh38.p14_Chr22:39312868-39312887).  56 is a number given by E-CRISPR \ 
ORIGIN
        1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 
       61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 
      121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 
      181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 
      241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 
      301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 
      361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 
      421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 
      481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 
      541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 
      601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 
      661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 
      721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 
      781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 
      841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 
      901 GTTTAAACGG GCCCTCTAGA CTCGAGctca agcttcgaat tctgcagtcg acggtaccga 
      961 gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat 
     1021 aattagaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa 
     1081 gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat 
     1141 gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg 
     1201 aaacaccgAG AAAGAGGCAA GATGTCAGgt tttagagcta gaaatagcaa gttaaaataa 
     1261 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt gaattcgcta 
     1321 gctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat 
     1381 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgatccggt gcctagagaa 
     1441 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg 
     1501 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt 
     1561 ttgccgccag aacacaggac cggttctaga gcgctgccac catggacaag aagtacagca 
     1621 tcggcctgga catcggcacc aactctgtgg gctgggccgt gatcaccgac gagtacaagg 
     1681 tgcccagcaa gaaattcaag gtgctgggca acaccgaccg gcacagcatc aagaagaacc 
     1741 tgatcggagc cctgctgttc gacagcggcg aaacagccga ggccacccgg ctgaagagaa 
     1801 ccgccagaag aagatacacc agacggaaga accggatctg ctatctgcaa gagatcttca 
     1861 gcaacgagat ggccaaggtg gacgacagct tcttccacag actggaagag tccttcctgg 
     1921 tggaagagga taagaagcac gagcggcacc ccatcttcgg caacatcgtg gacgaggtgg 
     1981 cctaccacga gaagtacccc accatctacc acctgagaaa gaaactggtg gacagcaccg 
     2041 acaaggccga cctgcggctg atctatctgg ccctggccca catgatcaag ttccggggcc 
     2101 acttcctgat cgagggcgac ctgaaccccg acaacagcga cgtggacaag ctgttcatcc 
     2161 agctggtgca gacctacaac cagctgttcg aggaaaaccc catcaacgcc agcggcgtgg 
     2221 acgccaaggc catcctgtct gccagactga gcaagagcag acggctggaa aatctgatcg 
     2281 cccagctgcc cggcgagaag aagaatggcc tgttcggaaa cctgattgcc ctgagcctgg 
     2341 gcctgacccc caacttcaag agcaacttcg acctggccga ggatgccaaa ctgcagctga 
     2401 gcaaggacac ctacgacgac gacctggaca acctgctggc ccagatcggc gaccagtacg 
     2461 ccgacctgtt tctggccgcc aagaacctgt ccgacgccat cctgctgagc gacatcctga 
     2521 gagtgaacac cgagatcacc aaggcccccc tgagcgcctc tatgatcaag agatacgacg 
     2581 agcaccacca ggacctgacc ctgctgaaag ctctcgtgcg gcagcagctg cctgagaagt 
     2641 acaaagagat tttcttcgac cagagcaaga acggctacgc cggctacatt gacggcggag 
     2701 ccagccagga agagttctac aagttcatca agcccatcct ggaaaagatg gacggcaccg 
     2761 aggaactgct cgtgaagctg aacagagagg acctgctgcg gaagcagcgg accttcgaca 
     2821 acggcagcat cccccaccag atccacctgg gagagctgca cgccattctg cggcggcagg 
     2881 aagattttta cccattcctg aaggacaacc gggaaaagat cgagaagatc ctgaccttcc 
     2941 gcatccccta ctacgtgggc cctctggcca ggggaaacag cagattcgcc tggatgacca 
     3001 gaaagagcga ggaaaccatc accccctgga acttcgagga agtggtggac aagggcgctt 
     3061 ccgcccagag cttcatcgag cggatgacca acttcgataa gaacctgccc aacgagaagg 
     3121 tgctgcccaa gcacagcctg ctgtacgagt acttcaccgt gtataacgag ctgaccaaag 
     3181 tgaaatacgt gaccgaggga atgagaaagc ccgccttcct gagcggcgag cagaaaaagg 
     3241 ccatcgtgga cctgctgttc aagaccaacc ggaaagtgac cgtgaagcag ctgaaagagg 
     3301 actacttcaa gaaaatcgag tgcttcgact ccgtggaaat ctccggcgtg gaagatcggt 
     3361 tcaacgcctc cctgggcaca taccacgatc tgctgaaaat tatcaaggac aaggacttcc 
     3421 tggacaatga ggaaaacgag gacattctgg aagatatcgt gctgaccctg acactgtttg 
     3481 aggacagaga gatgatcgag gaacggctga aaacctatgc ccacctgttc gacgacaaag 
     3541 tgatgaagca gctgaagcgg cggagataca ccggctgggg caggctgagc cggaagctga 
     3601 tcaacggcat ccgggacaag cagtccggca agacaatcct ggatttcctg aagtccgacg 
     3661 gcttcgccaa cagaaacttc atgcagctga tccacgacga cagcctgacc tttaaagagg 
     3721 acatccagaa agcccaggtg tccggccagg gcgatagcct gcacgagcac attgccaatc 
     3781 tggccggcag ccccgccatt aagaagggca tcctgcagac agtgaaggtg gtggacgagc 
     3841 tcgtgaaagt gatgggccgg cacaagcccg agaacatcgt gatcgaaatg gccagagaga 
     3901 accagaccac ccagaaggga cagaagaaca gccgcgagag aatgaagcgg atcgaagagg 
     3961 gcatcaaaga gctgggcagc cagatcctga aagaacaccc cgtggaaaac acccagctgc 
     4021 agaacgagaa gctgtacctg tactacctgc agaatgggcg ggatatgtac gtggaccagg 
     4081 aactggacat caaccggctg tccgactacg atgtggacca tatcgtgcct cagagctttc 
     4141 tgaaggacga ctccatcgac aacaaggtgc tgaccagaag cgacaagaac cggggcaaga 
     4201 gcgacaacgt gccctccgaa gaggtcgtga agaagatgaa gaactactgg cggcagctgc 
     4261 tgaacgccaa gctgattacc cagagaaagt tcgacaatct gaccaaggcc gagagaggcg 
     4321 gcctgagcga actggataag gccggcttca tcaagagaca gctggtggaa acccggcaga 
     4381 tcacaaagca cgtggcacag atcctggact cccggatgaa cactaagtac gacgagaatg 
     4441 acaagctgat ccgggaagtg aaagtgatca ccctgaagtc caagctggtg tccgatttcc 
     4501 ggaaggattt ccagttttac aaagtgcgcg agatcaacaa ctaccaccac gcccacgacg 
     4561 cctacctgaa cgccgtcgtg ggaaccgccc tgatcaaaaa gtaccctaag ctggaaagcg 
     4621 agttcgtgta cggcgactac aaggtgtacg acgtgcggaa gatgatcgcc aagagcgagc 
     4681 aggaaatcgg caaggctacc gccaagtact tcttctacag caacatcatg aactttttca 
     4741 agaccgagat taccctggcc aacggcgaga tccggaagcg gcctctgatc gagacaaacg 
     4801 gcgaaaccgg ggagatcgtg tgggataagg gccgggattt tgccaccgtg cggaaagtgc 
     4861 tgagcatgcc ccaagtgaat atcgtgaaaa agaccgaggt gcagacaggc ggcttcagca 
     4921 aagagtctat cctgcccaag aggaacagcg ataagctgat cgccagaaag aaggactggg 
     4981 accctaagaa gtacggcggc ttcgacagcc ccaccgtggc ctattctgtg ctggtggtgg 
     5041 ccaaagtgga aaagggcaag tccaagaaac tgaagagtgt gaaagagctg ctggggatca 
     5101 ccatcatgga aagaagcagc ttcgagaaga atcccatcga ctttctggaa gccaagggct 
     5161 acaaagaagt gaaaaaggac ctgatcatca agctgcctaa gtactccctg ttcgagctgg 
     5221 aaaacggccg gaagagaatg ctggcctctg ccggcgaact gcagaaggga aacgaactgg 
     5281 ccctgccctc caaatatgtg aacttcctgt acctggccag ccactatgag aagctgaagg 
     5341 gctcccccga ggataatgag cagaaacagc tgtttgtgga acagcacaag cactacctgg 
     5401 acgagatcat cgagcagatc agcgagttct ccaagagagt gatcctggcc gacgctaatc 
     5461 tggacaaagt gctgtccgcc tacaacaagc accgggataa gcccatcaga gagcaggccg 
     5521 agaatatcat ccacctgttt accctgacca atctgggagc ccctgccgcc ttcaagtact 
     5581 ttgacaccac catcgaccgg aagaggtaca ccagcaccaa agaggtgctg gacgccaccc 
     5641 tgatccacca gagcatcacc ggcctgtacg agacacggat cgacctgtct cagctgggag 
     5701 gcgacaagcg acctgccgcc acaaagaagg ctggacaggc taagaagaag aaagattaca 
     5761 aagacgatga cgataagGGA TCCggcgcaa caaacttctc tctgctgaaa caagccggag 
     5821 atgtcgaaga gaatcctgga ccgaccgagt acaagcccac ggtgcgcctc gccacccgcg 
     5881 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc 
     5941 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc 
     6001 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg 
     6061 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc 
     6121 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg 
     6181 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggagtc tcgcccgacc 
     6241 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg 
     6301 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc 
     6361 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgCATGA 
     6421 CCCGCAAGCC CGGTGCCTGA CTTAAGTTTA AACCGCTGAT CAGCCTCGAC TGTGCCTTCT 
     6481 AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC 
     6541 ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT 
     6601 CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT 
     6661 AGCAGGCATG CTGGGGATGC GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG AACCAGCTGG 
     6721 GGCTCTAGGG GGTATCCCCA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     6781 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     6841 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     6901 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     6961 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     7021 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     7081 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     7141 CTGATTTAAC AAAAATTTAA CGCGAATTAA TTCTGTGGAA TGTGTGTCAG TTAGGGTGTG 
     7201 GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG 
     7261 CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC 
     7321 TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC 
     7381 CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG 
     7441 AGGCCGCCTC TGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG 
     7501 GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG 
     7561 GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT 
     7621 GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG 
     7681 CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG 
     7741 GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG 
     7801 TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG 
     7861 GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA 
     7921 TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC 
     7981 ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC 
     8041 AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA 
     8101 AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA 
     8161 ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG 
     8221 CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG 
     8281 AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG 
     8341 CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA 
     8401 CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG 
     8461 GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT 
     8521 CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA 
     8581 AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG 
     8641 TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG 
     8701 CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC 
     8761 ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA 
     8821 ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA 
     8881 GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC 
     8941 CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC 
     9001 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     9061 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     9121 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     9181 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     9241 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     9301 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     9361 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     9421 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     9481 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     9541 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     9601 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTTTTTT 
     9661 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     9721 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     9781 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     9841 CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC 
     9901 TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT 
     9961 AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC 
    10021 ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG 
    10081 AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG 
    10141 AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT 
    10201 GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG 
    10261 AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT 
    10321 TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC 
    10381 TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC 
    10441 ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA 
    10501 TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG 
    10561 AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC 
    10621 CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG 
    10681 GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT 
    10741 CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT 
    10801 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC 
    10861 ACCTGACGTC 
//