LOCUS       Plasmidsaurus-P2_proph 33287 bp ds-DNA     linear       24-AUG-2023
DEFINITION  .
KEYWORDS    "creator:Jaramillo Lab" "marker:gBlock-epsilon_in_genome-hygR,
            P2-gpR, R" "organism:Escherichia coli C"
FEATURES             Location/Qualifiers
     variation       3..690
                     /label="del6 deletion"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: del6 deletion"
     variation       3..1552
                     /label="P2 vir22 deletion/insertion"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: vir22 deletion/insertion"
     variation       6..1965
                     /label="vir94 deletion/insertion"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: vir94 deletion/insertion"
     protein_bind    24..97
                     /label="P2 Cox binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: Cox"
     gene            complement(87..1100)
                     /label="int"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: int"
     CDS             complement(87..1100)
                     /label="P2-int"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GI: 3139116 function: integrase gene: int product: Int protein_id: AAD03297.1 transl_table: 1 translation: MAIKKLDDGRYEVDIRPTGRNGKRIRRKFDKKSEAVAFEKYTLYNHHNKEWLSKPTDKRRLSELTQIWWDLKGKHEEHGKSNLGKIEIFTKITNDPCAFQITKSLISQYCATRRSQGIKPSSINRDLTCISGMFTALIEAELFFGEHPIRGTKRLKEEKPETGYLTQEEIALLLAALDGDNKKIAILCLSTGARWGEAARLKAENIIHNRVTFVKTKTNKPRTVPISEAVAKMIADNKRGFLFPDADYPRFRRTMKAIKPDLPMGQATHALRHSFATHFMINGGSIITLQRILGHTRIEQTMVYAHFAPEYLQDAISLNPLRGGTEAESVHTVSTVE*"
     protein_bind    95..121
                     /label="P2 Int binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: Int note: P' arm"
     variation       1117..1464
                     /label="vir 79 deletion"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: vir 79 deletion"
     CDS             complement(1216..1515)
                     /label="P2-gpC"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GI: 3139117 function: immunity repressor gene: C product: gpC protein_id: AAD03298.1 transl_table: 1 translation: MSNTISEKIVLMRKSEYLSRQQLADLTGVPYGTLSYYESGRSTPPTDVMMNILQTPQFTKYTLWFMTNQIAPESGQIAPALAHFGQNETTSPHSGQKTG*"
     gene            complement(1216..1515)
                     /label="C"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: C"
     variation       1311..1327
                     /label="cam11 and cam13 mutations"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: C note: cam11 and cam13 mutations replace: a"
     promoter        1499..1508
                     /label="P2-pE promoter"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_fwdcolor="#800000"
                     /note="note: strong promoter pE from phage P2 is repressed in the original strain E811 (P2 lygogen). O1/O2 repressor binding sites marked"
     promoter        1509..1514
                     /label="P2-pE promoter"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_fwdcolor="#800000"
                     /note="note: strong promoter pE from phage P2 is repressed in the original strain E811 (P2 lygogen). O1/O2 repressor binding sites marked"
     promoter        1515..1518
                     /label="P2-pE promoter"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_fwdcolor="#800000"
                     /note="note: strong promoter pE from phage P2 is repressed in the original strain E811 (P2 lygogen). O1/O2 repressor binding sites marked"
     promoter        1519..1526
                     /label="P2-pE promoter"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_fwdcolor="#800000"
                     /note="note: strong promoter pE from phage P2 is repressed in the original strain E811 (P2 lygogen). O1/O2 repressor binding sites marked"
     misc_feature    1519..1526
                     /label="O1 repressor binding site"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: O1 repressor binding site"
     misc_feature    1519..1526
                     /label="O2 repressor binding site"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: O2 repressor binding site"
     variation       1524..1580
                     /label="vir24 deletion"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: vir24 deletion"
     variation       1525..1541
                     /label="vir3 deletion"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: vir3 deletion"
     promoter        1527..1531
                     /label="P2-pE promoter"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_fwdcolor="#800000"
                     /note="note: strong promoter pE from phage P2 is repressed in the original strain E811 (P2 lygogen). O1/O2 repressor binding sites marked"
     variation       1529..1545
                     /label="vir65 deletion"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: vir65 deletion"
     promoter        1532..1537
                     /label="P2-pE promoter"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_fwdcolor="#800000"
                     /note="note: strong promoter pE from phage P2 is repressed in the original strain E811 (P2 lygogen). O1/O2 repressor binding sites marked"
     variation       1534..1608
                     /label="vir40 deletion"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: vir40 deletion"
     promoter        1538..1540
                     /label="P2-pE promoter"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_fwdcolor="#800000"
                     /note="note: strong promoter pE from phage P2 is repressed in the original strain E811 (P2 lygogen). O1/O2 repressor binding sites marked"
     promoter        1541..1548
                     /label="P2-pE promoter"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_fwdcolor="#800000"
                     /note="note: strong promoter pE from phage P2 is repressed in the original strain E811 (P2 lygogen). O1/O2 repressor binding sites marked"
     misc_feature    1541..1548
                     /label="O2 repressor binding site"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: O2 repressor binding site"
     misc_feature    1541..1548
                     /label="O1 repressor binding site"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: O1 repressor binding site"
     promoter        1549..1567
                     /label="P2-pE promoter"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_fwdcolor="#800000"
                     /note="note: strong promoter pE from phage P2 is repressed in the original strain E811 (P2 lygogen). O1/O2 repressor binding sites marked"
     promoter        complement(1554..1586)
                     /label="P2-pC promoter"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_fwdcolor="#800000"
                     /note="note: P2 phage pC promoter initially thought [1] to be activated by P2-C repressor (x4-5 fold) when Cis at low levels, but repressed by C (x0.5 fold) if at higher levels. In reallity, this is due to the regulation of the pE promoter [3], so I had to add it. The predicted activation in a P2 lysogen is x8 fold with a 5% leakage[3].Cox sterically hinders the RNA-polymerase to bind to the Pc promoter of P2 and thereby repress transcription, since the Cox boxes are covering the promoter (Berntsson et al.). 1. Saha S, Lundqvist B, Haggård-Ljungquist E. Autoregulation of bacteriophage P2 repressor. The EMBO Journal. 1987 Mar 1;6(3):809–14. 2. Berntsson RPA, Odegrip R, Sehlén W, Skaar K, Svensson LM, Massad T, Högbom M, Haggård-Ljungquist E, Stenmark P. Structural insight into DNA binding and oligomerization of the multifunctional Cox protein of bacteriophage P2. Nucleic Acids Res. 2nd ed. 2013 Nov 19;42(4):2725–35.3. Saha S, Haggård-Ljungquist E, Nordström K. The cox protein of bacteriophage P2 inhibits the formation of the repressor protein and autoregulates the early operon. The EMBO Journal. 1987 Oct 1;6(10):3191–9. "
     promoter        complement(1587..1592)
                     /label="P2-pC promoter"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_fwdcolor="#800000"
                     /note="note: P2 phage pC promoter initially thought [1] to be activated by P2-C repressor (x4-5 fold) when Cis at low levels, but repressed by C (x0.5 fold) if at higher levels. In reallity, this is due to the regulation of the pE promoter [3], so I had to add it. The predicted activation in a P2 lysogen is x8 fold with a 5% leakage[3].Cox sterically hinders the RNA-polymerase to bind to the Pc promoter of P2 and thereby repress transcription, since the Cox boxes are covering the promoter (Berntsson et al.). 1. Saha S, Lundqvist B, Haggård-Ljungquist E. Autoregulation of bacteriophage P2 repressor. The EMBO Journal. 1987 Mar 1;6(3):809–14. 2. Berntsson RPA, Odegrip R, Sehlén W, Skaar K, Svensson LM, Massad T, Högbom M, Haggård-Ljungquist E, Stenmark P. Structural insight into DNA binding and oligomerization of the multifunctional Cox protein of bacteriophage P2. Nucleic Acids Res. 2nd ed. 2013 Nov 19;42(4):2725–35.3. Saha S, Haggård-Ljungquist E, Nordström K. The cox protein of bacteriophage P2 inhibits the formation of the repressor protein and autoregulates the early operon. The EMBO Journal. 1987 Oct 1;6(10):3191–9. "
     promoter        complement(1593..1606)
                     /label="P2-pC promoter"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_fwdcolor="#800000"
                     /note="note: P2 phage pC promoter initially thought [1] to be activated by P2-C repressor (x4-5 fold) when Cis at low levels, but repressed by C (x0.5 fold) if at higher levels. In reallity, this is due to the regulation of the pE promoter [3], so I had to add it. The predicted activation in a P2 lysogen is x8 fold with a 5% leakage[3].Cox sterically hinders the RNA-polymerase to bind to the Pc promoter of P2 and thereby repress transcription, since the Cox boxes are covering the promoter (Berntsson et al.). 1. Saha S, Lundqvist B, Haggård-Ljungquist E. Autoregulation of bacteriophage P2 repressor. The EMBO Journal. 1987 Mar 1;6(3):809–14. 2. Berntsson RPA, Odegrip R, Sehlén W, Skaar K, Svensson LM, Massad T, Högbom M, Haggård-Ljungquist E, Stenmark P. Structural insight into DNA binding and oligomerization of the multifunctional Cox protein of bacteriophage P2. Nucleic Acids Res. 2nd ed. 2013 Nov 19;42(4):2725–35.3. Saha S, Haggård-Ljungquist E, Nordström K. The cox protein of bacteriophage P2 inhibits the formation of the repressor protein and autoregulates the early operon. The EMBO Journal. 1987 Oct 1;6(10):3191–9. "
     promoter        complement(1607..1612)
                     /label="P2-pC promoter"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_fwdcolor="#800000"
                     /note="note: P2 phage pC promoter initially thought [1] to be activated by P2-C repressor (x4-5 fold) when Cis at low levels, but repressed by C (x0.5 fold) if at higher levels. In reallity, this is due to the regulation of the pE promoter [3], so I had to add it. The predicted activation in a P2 lysogen is x8 fold with a 5% leakage[3].Cox sterically hinders the RNA-polymerase to bind to the Pc promoter of P2 and thereby repress transcription, since the Cox boxes are covering the promoter (Berntsson et al.). 1. Saha S, Lundqvist B, Haggård-Ljungquist E. Autoregulation of bacteriophage P2 repressor. The EMBO Journal. 1987 Mar 1;6(3):809–14. 2. Berntsson RPA, Odegrip R, Sehlén W, Skaar K, Svensson LM, Massad T, Högbom M, Haggård-Ljungquist E, Stenmark P. Structural insight into DNA binding and oligomerization of the multifunctional Cox protein of bacteriophage P2. Nucleic Acids Res. 2nd ed. 2013 Nov 19;42(4):2725–35.3. Saha S, Haggård-Ljungquist E, Nordström K. The cox protein of bacteriophage P2 inhibits the formation of the repressor protein and autoregulates the early operon. The EMBO Journal. 1987 Oct 1;6(10):3191–9. "
     promoter        complement(1613..1622)
                     /label="P2-pC promoter"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_fwdcolor="#800000"
                     /note="note: P2 phage pC promoter initially thought [1] to be activated by P2-C repressor (x4-5 fold) when Cis at low levels, but repressed by C (x0.5 fold) if at higher levels. In reallity, this is due to the regulation of the pE promoter [3], so I had to add it. The predicted activation in a P2 lysogen is x8 fold with a 5% leakage[3].Cox sterically hinders the RNA-polymerase to bind to the Pc promoter of P2 and thereby repress transcription, since the Cox boxes are covering the promoter (Berntsson et al.). 1. Saha S, Lundqvist B, Haggård-Ljungquist E. Autoregulation of bacteriophage P2 repressor. The EMBO Journal. 1987 Mar 1;6(3):809–14. 2. Berntsson RPA, Odegrip R, Sehlén W, Skaar K, Svensson LM, Massad T, Högbom M, Haggård-Ljungquist E, Stenmark P. Structural insight into DNA binding and oligomerization of the multifunctional Cox protein of bacteriophage P2. Nucleic Acids Res. 2nd ed. 2013 Nov 19;42(4):2725–35.3. Saha S, Haggård-Ljungquist E, Nordström K. The cox protein of bacteriophage P2 inhibits the formation of the repressor protein and autoregulates the early operon. The EMBO Journal. 1987 Oct 1;6(10):3191–9. "
     gene            1630..1905
                     /label="cox"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: cox"
     CDS             1630..1905
                     /label="P2-cox"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GI: 3139118 function: repressor of Pc; required for prophageexcision; inhibits integration; activates P11 promoter of satellite phage P4 gene: cox product: Cox protein_id: AAD03299.1 transl_table: 1 translation: MSKQVTLMTDAIPYQEFAKLIGKSTGAVRRMIDKGKLPVIDMTDPQSASGRAGEYWVYLPAWNNGLKLAYESRPKEIRDGWLMWLGLGEPR*"
     gene            1916..2086
                     /label="orf78"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: orf78"
     gene            2083..2583
                     /label="B"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: B"
     CDS             2083..2583
                     /label="P2-gpB"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GI: 3139120 function: essential protein; DNA replication; requiredfor lagging strand synthesis gene: B product: gpB protein_id: AAD03301.1 transl_table: 1 translation: MTVMTLNLVEKQPAAMRRIIGKHLAVPRWQDTCDYYNQMMERERLTVCFHAQLKQRHATMCFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLTVSQRRTLFMHAGLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVKPEQYLH*"
     regulatory      2605..2632
                     /label="P2-B terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: putative rho-independent terminator regulatory_class: terminator: "
     gene            2647..2871
                     /label="orf80"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: orf80"
     CDS             2871..3173
                     /label="orf81"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GI: 3139122 gene: orf81 product: Orf81 protein_id: AAD03303.1 transl_table: 1 translation: MFCSRAVVLLNNALKIAVMKNGDLSLIQLGLDKEKREITESVIAIYQSELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTEIASYCADEFKKLNDKRNW*"
     gene            3173..3397
                     /label="orf82"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: orf82"
     gene            3394..3669
                     /label="orf83"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: orf83"
     CDS             3659..5944
                     /label="P2-gpA"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GI: 3139125 function: essential protein; DNA replication; makessite-specific nick at origin of replication gene: A product: gpA protein_id: AAD03306.1 transl_table: 1 translation: MAVKASGRFVPPSAFAAGTGKMFTGAYAWNAPREAVGRERPLTRDEMRQMQGVLSTINRLPYFLRSLFTSRYDYIRRNKSPVHGFYFLTSTFQRRLWPRIERVNQRHEMNTDASLLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYEELSDAWVDAHGEKESLFTDEAQAHLYGHVAGAARAFNISPLYWKKYRKGQMTTRQAYSAIARLFNDEWWTHQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLENRETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTAPSKYHPTRQVGKGESKTVQLNHGWNDEAFNPKDAQRYLCHIWSLMRTAFKDNDLQVYGLRVVEPHHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNQGGAAGYIAKYISKNIDGYALDGQLDNDTGRPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRELRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSDEVNEYEEEVERVVGIYAPHLGARHIHITRTTDWRIVPKVPVVEPLTLKSGIAAPRSPVNNCGKLTGGDTSLPAPTPSEHAAAVLNLVDDGVIEWNEPEVVRALRGALKYDMRTPNRQQRNGSPLKPHEIAPSARLTRSERLQITRIRVDLAQNGIRPQRWELEALARGATVNYDGKKFTYPVADEWPGFSTVMEWT*"
     CDS             5941..6270
                     /label="orf91"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GI: 3139126 gene: orf91 product: Orf91 protein_id: AAD03307.1 transl_table: 1 translation: MMAKIHEVKLHAKYFDLVLEGKKRAEFRKKDRNYERGDTLILHEWVQGVFTGRKVEARITDVTDLSDWLEDYVLLHRILGEILPLTVKLAVSSTFFINLRRRTVAIYLL*"
     CDS             complement(6309..6646)
                     /label="tin"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GI: 3139127 function: nonessential protein; lysogenic conversion;blocks growth of T-even phages gene: tin product: Tin protein_id: AAD03308.1 transl_table: 1 translation: MNNMDIVKAVKDGMKKSADATYLMDFRSGAKINTEYVATVSIGLSLLEIKSFRHGDYKVIFEYHTNKFINATVPLSKRSDPQKIFSKKTVRKNTNTTRSGRIDIAILDSRPFFDIPICAIEVKGNAPCKSLLFSDIRRNLEYFKHTGPTGNSSLGLALNCSFHSYNDSTKKNYCTTIHHKEDMIRKLKNKYKKYISELNEEIPDDISVTIDVFTAAEHLLSPDADQYEYESHIDDLHLTLGVMVIFERKSILN*"
     variation       6617..6646
                     /label="del4 deletion"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: del4 deletion"
     terminator      6733..6789
                     /label="L3S2P55 strong terminator"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_fwdcolor="#800000"
                     /note="note: Recombination resistant. Strength 255 = 2.3x rrnCChen Y-J, Liu P, Nielsen AAK, Brophy JAN, Clancy K, Peterson T, Voigt CA. Characterization of 582 natural and synthetic terminators and quantification of their design constraints. Nat Meth. 2013 Jun 2;10(7):659–64. "
     promoter        6790..6807
                     /label="rhaB promoter"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_fwdcolor="#800000"
                     /note="note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)"
     promoter        6808..6823
                     /label="rhaB promoter"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)"
     promoter        6824..6826
                     /label="rhaB promoter"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_fwdcolor="#800000"
                     /note="note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)"
     promoter        6827..6843
                     /label="rhaB promoter"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)"
     promoter        6844..6859
                     /label="rhaB promoter"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_fwdcolor="#800000"
                     /note="note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)"
     promoter        6860..6876
                     /label="rhaB promoter"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)"
     promoter        6877..6907
                     /label="rhaB promoter"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_fwdcolor="#800000"
                     /note="note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006) Mutation nucleotide C9318A in promoter; induction with rhamnosa has been tested to amplified a P4 cosmid and it has worked."
     promoter        6908..6908
                     /label="rhaB promoter"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_fwdcolor="#800000"
                     /note="note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)"
     RBS             6909..6915
                     /label="BBa_B0034 Strong RBS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="label: BBa_B0034_RBS"
     RBS             6916..6927
                     /label="BBa_B0034 Strong RBS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="label: BBa_B0034_RBS"
     RBS             6928..6933
                     /label="BBa_B0034 Strong RBS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="label: BBa_B0034_RBS"
     gene            6934..6937
                     /label="P4p12"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             6934..7434
                     /label="P4-gp13 delta"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GOA: P12551 db_xref: UniProtKB/Swiss-Prot: P12551 db_xref: GeneID: 1261088 locus_tag: P4p13 note: delta gene product (AA 1-166). This is a positive regulator that can both activate the satellite and trans-activate the helper late operons.Dehò, Gianni, and D. A. N. I. E. L. A. Ghisotti. ""The satellite phage P4."" The Bacteriophages (2006): 391-408. product: transactivation protein protein_id: NP_042043.1 transl_table: 1 translation: MIYCPSCGHVAHTRRAHFMDDGTKIMIAQCRNIYCSATFEASESFFSDSKDSGMEYISGKQRYRDSLTSASCGMKRPKRMLVTGYCCRRCKGLALSRTSRRLSQEVTERFYVCTDPGCGLVFKTLQTINRFIVRPVTPDELAERLHEKQELPPVRLKTQSYSLRLE*"
     source          6934..7434
                     /label="source"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="mol_type: genomic DNA: "
     misc_feature    7116..7116
                     /label="fkn insertion"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    7358..7437
                     /label="HL-epsilon"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    7358..9071
                     /label="gBlock-epsilon_in_genome-hygR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     RBS             7438..7461
                     /label="BBa_B0064 weak RBS"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     CDS             7462..7749
                     /label="P4 epsilon opt"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="codon_start: 1 note: epsilon from P4 phage with methionine as start codon and codon optimized for GC=60% using COOL. The opt will remove endogenous RNA regulations known to occur with epsilon. Alfonsohttps://cool.syncti.org Mutation: there is a non-silence change in a nucleotide, L41P, phenotypic results are unknown transl_table: 1 translation: MRNKKAPQTVSARHDAREHLSIEAYHKLNRASAVSRFVGGDLIHRELSGLHQLYIPHIFSYLNEDIDFVLNELKAKGLCRDFLAQQKDRGDRTHV*"
     CDS             7462..7749
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MRNKKAPQTVSARHDAREHLSIEAYHKLNRASAVSRFVGGDPIHRELSGLHQLYIPHIFSYLNEDIDFVLNELKAKGLCRDFLAQQKDRGDRTHV*"
     terminator      7753..7785
                     /label="BBa_B1002 terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: BBa_B1002"
     protein_bind    7786..7787
                     /label="FRT (in-frame)"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="allele: The first amino acid will by GLY (although with 0.9% usage in E. coli) bound_moiety: FLP recombinase from the Saccharomyces cerevisiae 2μ plasmid note: supports FLP-mediated excision but not integration (Turan and Bode, 2011)The first amino acid will by GLY (although with 0.9% usage in E. coli)"
     gene            7786..8991
                     /label="B6N50_08665"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="locus_tag: B6N50_08665"
     protein_bind    7788..7821
                     /label="FRT (minimal)"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: FLP recombinase from the Saccharomyces cerevisiae 2μ plasmid note: supports FLP-mediated excision but not integration (Turan and Bode, 2011)"
     protein_bind    7788..7821
                     /label="FRT (in-frame)"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="allele: The first amino acid will by GLY (although with 0.9% usage in E. coli) bound_moiety: FLP recombinase from the Saccharomyces cerevisiae 2μ plasmid note: supports FLP-mediated excision but not integration (Turan and Bode, 2011)The first amino acid will by GLY (although with 0.9% usage in E. coli)"
     promoter        7822..7856
                     /label="J23102 promoter"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_fwdcolor="#800000"
                     /note="note: BBa_J23102 http://parts.igem.org/Promoters/Catalog/Andersonstrength = 0.86 of BBa_J23100"
     CDS             7874..8878
                     /label="Hygro R"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="Gene that confers resistance to Hygromicin. Mutations from original: R64A and A263T. The strain has been grown in hygromicin plates (50ug/ml) and the gene seems to function. "
     CDS             7874..8878
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRTYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPG*"
     terminator      8907..8955
                     /label="L3S3P22 strong terminator"
                     /ApEinfo_revcolor="#993300"
                     /ApEinfo_fwdcolor="#993300"
                     /note="note: table {mso-displayed-decimal-separator:""\.""; mso-displayed-thousand-separator:""\,"";} @page {margin:.75in .7in .75in .7in; mso-header-margin:.3in; mso-footer-margin:.3in;} td {padding-top:1px; padding-right:1px; padding-left:1px; mso-ignore:padding; color:black; font-size:11.0pt; font-weight:400; font-style:normal; text-decoration:none; font-family:Calibri, sans-serif; mso-font-charset:0; mso-number-format:General; text-align:general; vertical-align:bottom; border:none; mso-background-source:auto; mso-pattern:auto; mso-protection:locked visible; white-space:nowrap; mso-rotate:0;} .xl63 {color:#000090; font-family:Calibri; mso-generic-font-family:auto; mso-font-charset:0; mso-number-format:Fixed; text-align:right;} StartFragment Strong terminator (strength= 231.63, 2.2x rrnC terminator ) resistant to recombination. Chen Y-J, Liu P, Nielsen AAK, Brophy JAN, Clancy K, Peterson T, Voigt CA. Characterization of 582 natural and synthetic terminators and quantification of their design constraints. Nat Meth. 2013 Jun 2;10(7):659–664.EndFragment"
     protein_bind    8956..8957
                     /label="FRT (in-frame)"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="allele: The first amino acid will by GLY (although with 0.9% usage in E. coli) bound_moiety: FLP recombinase from the Saccharomyces cerevisiae 2μ plasmid note: supports FLP-mediated excision but not integration (Turan and Bode, 2011)The first amino acid will by GLY (although with 0.9% usage in E. coli)"
     protein_bind    8958..8991
                     /label="FRT (minimal)"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: FLP recombinase from the Saccharomyces cerevisiae 2μ plasmid note: supports FLP-mediated excision but not integration (Turan and Bode, 2011)"
     protein_bind    8958..8991
                     /label="FRT (in-frame)"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="allele: The first amino acid will by GLY (although with 0.9% usage in E. coli) bound_moiety: FLP recombinase from the Saccharomyces cerevisiae 2μ plasmid note: supports FLP-mediated excision but not integration (Turan and Bode, 2011)The first amino acid will by GLY (although with 0.9% usage in E. coli)"
     misc_feature    8992..9071
                     /label="Homology Right C-117"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     regulatory      complement(9072..9093)
                     /label="regulatory region"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: tQ; putative rho-independent terminator for Poperon regulatory_class: terminator: "
     gene            complement(9099..10133)
                     /label="Q"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: Q"
     CDS             complement(9099..10133)
                     /label="P2-gpQ"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GI: 3139087 experiment: experimental evidence, no additional detailsrecorded function: presumed portal protein gene: Q product: gpQ protein_id: AAD03268.1 transl_table: 1 translation: MSKKKGKTPQPAAKTMTASGPKMEAFTFGEPVPVLDRRDILDYVECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFVLDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTAFAPGSVFHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDRNDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAADLLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRFKNYSLDTDND*"
     CDS             complement(10133..11905)
                     /label="P2-gpP"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GI: 3139088 experiment: experimental evidence, no additional detailsrecorded function: terminase; DNA-dependent ATPase gene: P product: gpP protein_id: AAD03269.1 transl_table: 1 translation: MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSWKQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQTGNEADLNPNVANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFDYQLHWYRAGLEHRIRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVDLTGDPIVLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSHLRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQIVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKASVFPFEELQRCMVDTLEEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGMDFATQAESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVLKAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATMHALLNEPLTAGISTPLTSTILEFY*"
     gene            complement(10133..11928)
                     /label="P2-gpP"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: P"
     promoter        11947..12038
                     /label="P2-PO promoter"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_fwdcolor="#800000"
                     /note="note: PO promoter from P2 phageThis is the PO promoter taken from the P2 phage genome. It is an inducible promoter that is activated by a class of activators, including P2 ogr (I746350), PSP3 pag (I746351) and phiR73 delta (I746352). These different activators should cause different levels of activity of the PO promoter.Part:BBa_I746361"
     misc_feature    11969..11999
                     /label="miscellaneous"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="experiment: experimental evidence, no additional detailsrecorded note: activator binding site; pO, pP promoters"
     CDS             12079..12933
                     /label="P2-gpO"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GI: 3139089 function: presumed capsid scaffolding protein gene: O product: gpO protein_id: AAD03270.1 transl_table: 1 translation: MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRINLEHLRGILPDGIFKRYGDVAELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQKVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENLISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHEAVTAVAEHVQEKLSATEQRLAEMETAFSALKQEVTDRADETSQAFTRLKNSLDHTESLTQQRRSKATGGGGDALMTNC*"
     gene            12079..12933
                     /label="O"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: O"
     gene            12992..14065
                     /label="N"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: N"
     CDS             12992..14065
                     /label="P2-gpN"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GI: 3139090 function: major capsid precursor gene: N product: gpN protein_id: AAD03271.1 transl_table: 1 translation: MRQETRFKFNAYLSRVAELNGIDAGDVSKKFTVEPSVTQTLMNTMQESSDFLTRINIVPVSEMKGEKIGIGVTGSIASTTDTAGGTERQPKDFSKLASNKYECDQINFDFYIRYKTLDLWARYQDFQLRIRNAIIKRQSLDFIMAGFNGVKRAETSDRSSNPMLQDVAVGWLQKYRNEAPARVMSKVTDEEGRTTSEVIRVGKGGDYASLDALVMDATNNLIEPWYQEDPDLVVIVGRQLLADKYFPIVNKEQDNSEMLAADVIISQKRIGNLPAVRVPYFPADAMLITKLENLSIYYMDDSHRRVIEENPKLDRVENYESMNIDYVVEDYAAGCLVEKIKVGDFSTPAKATAEPGA*"
     CDS             14069..14812
                     /label="P2-gpM"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GI: 3139091 function: terminase gene: M product: gpM protein_id: AAD03272.1 transl_table: 1 translation: MTSPAQRHMMRVSAAMTAQREAAPLRHATVYEQMLVKLAADQRTLKAIYSKELKAAKKRELLPFWLPWVNGVLELGKGAQDDILMTVMLWRLDTGDIAGALEIARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDTRLLTETLELTATADMPDEVRAKLHKITGLFLRDGGDAAGALAHLQRATQLDCQAGVKKEIERLERELKPKPEPQPKAATRAPRKTRSVTPAKRGRPKKKAS*"
     gene            14069..14812
                     /label="M"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: M"
     gene            14912..15421
                     /label="L"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: L"
     CDS             14912..15421
                     /label="P2-gpL"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GI: 3139092 function: capsid completion protein gene: L product: gpL protein_id: AAD03273.1 transl_table: 1 translation: MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKRVRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFTRLADVPADDIDGESIKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQGKPRCIVSQI*"
     gene            15421..15624
                     /label="X"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: X"
     CDS             15421..15624
                     /label="P2-gpX"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GI: 3139093 experiment: experimental evidence, no additional detailsrecorded function: essential tail gene gene: X product: gpX protein_id: AAD03274.1 transl_table: 1 translation: MKTFALQGDTLDAICVRYYGRTEGVVETVLAANPGLAELGAVLPHGTAVELPDVQTAPVAETVNLWE*"
     gene            15628..15909
                     /label="Y"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: Y"
     CDS             15628..15909
                     /label="P2-gpY"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GI: 3139094 experiment: experimental evidence, no additional detailsrecorded function: holin; essential for host cell cell lysis gene: Y product: gpY protein_id: AAD03275.1 transl_table: 1 translation: MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGFVSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKQ*"
     gene            15909..16406
                     /label="K"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: K"
     CDS             15909..16406
                     /label="P2-gpK"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GI: 3139095 experiment: experimental evidence, no additional detailsrecorded function: endolysin; essential for host cell lysis gene: K product: gpK protein_id: AAD03276.1 transl_table: 1 translation: MPVINTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGKPEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQDRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTAGGVPA*"
     gene            16421..16846
                     /label="lysA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: lysA"
     CDS             16421..16846
                     /label="P2-lysA"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GI: 3139096 experiment: experimental evidence, no additional detailsrecorded function: nonessential; affects timing of lysis gene: lysA product: LysA protein_id: AAD03277.1 transl_table: 1 translation: MKKLSLSLMLNVSLALMLALSLIYPQSVAVNFVAAWAILATVICVVAGGVGVYATEYVLERYGRELPPESLAVKIVTSLFLQPVPWRRRAAALVVVVATFISLVAAGWIFTALIYLVVSLFFRLIRKACRQRLEGREPCQG*"
     gene            16834..17259
                     /label="lysB"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: lysB"
     CDS             16834..17259
                     /label="P2 lysB"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GI: 3139097 experiment: experimental evidence, no additional detailsrecorded function: nonessential; affects timing of lysis gene: lysB product: LysB protein_id: AAD03278.1 transl_table: 1 translation: MSRLMIVLVVLLSLAVAGLFLVKHKNASLRASLDRANNVASGQQTTITMLKNQLHVALTRADKNELAQVALRQELENAAKREAQREKTITRLLNENEDFRRWYGADLPDAVRRLHQRPACTDASDCPQRMPESEPLPDAGQ*"
     CDS             17114..17404
                     /label="P2-lysC"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GI: 21956655 experiment: experimental evidence, no additional detailsrecorded gene: lysC note: non-essential; regulation of lysis product: LysC protein_id: AAM83596.1 transl_table: 1 translation: MRTKIFAAGTVLTCLMLCAGCTSAPPAPTPVIVPNACPKVSLCPMPGSDPQTNGDLSADIRQLENALARCASQVKMIKHCQDENDAQTRQPAQGAD*"
     gene            17114..17404
                     /label="lysC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: lysC"
     regulatory      17311..17329
                     /label="regulatory region"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: lysC note: putative transcription attenuator; O operon regulatory_class: attenuator: "
     CDS             17367..17834
                     /label="P2-gpR"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GI: 3139098 experiment: experimental evidence, no additional detailsrecorded function: essential tail gene; tail completion gene: R product: gpR protein_id: AAD03279.1 transl_table: 1 translation: MLKPDSLRRALTDAVTVLKTNPDMLRIFVDNGSIASTLAASLSFEKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDEGQKKGFTFYADINNDSSFDISISLMLTERTLVSEVDGALHVKNISEPPPPEPVTRPMELYINGELVSKWDE*"
     gene            17367..17834
                     /label="R"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: R"
     gene            17827..18279
                     /label="S"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: S"
     CDS             17827..18279
                     /label="P2-gpS"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GI: 3139099 experiment: experimental evidence, no additional detailsrecorded function: essential tail gene; tail completion gene: S product: gpS protein_id: AAD03280.1 transl_table: 1 translation: MNEFKRFEDRLTGLIESLSPSGRRRLSAELAKRLRQSQQRRVMAQKAPDGTPYAPRQQQSVRKKTGRVKRKMFAKLITSRFLHIRASPEQASMEFYGGKSPKIASVHQFGLSEENRKDGKKIDYPARPLLGFTGEDVQMIEEIILAHLER*"
     regulatory      18309..18345
                     /label="putative rho-independent terminator for O operon"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: putative rho-independent terminator for O operon regulatory_class: terminator: "
     CDS             complement(18351..19136)
                     /label="orf30"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GI: 3139100 function: nonessential gene: orf30 product: Orf30 protein_id: AAD03281.1 transl_table: 1 translation: MVSYNVTNVWGLIVFFLCSFAVLAFFSFGKSNLMRLIAHYFNFGYSDKKLKRLDREWRDIQLFKIINGINVSGIENVRMIQQGLIDGKLKTSYFFLTRIWGDITKPPHIIKTIIVILASIFYILLACYIHNEQSVIVRDATGIPYKNMMYYVYSDKVLLSFKNKAVEFNKTYSLADCKRLQNVFIKDTLPEIACNKLLQLNEEDSEWLSQEIKDNNSHKKALLILSLVYFTSGLVIFLSYTKFFYANKKVLEYKASNKNHS*"
     misc_feature    19151..19178
                     /label="miscellaneous"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="experiment: experimental evidence, no additional detailsrecorded note: activator binding site; activation of pV promoter"
     gene            19220..19855
                     /label="V"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: V"
     CDS             19220..19855
                     /label="P2-gpV"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GI: 3139101 experiment: experimental evidence, no additional detailsrecorded function: baseplate assembly protein gene: V product: gpV protein_id: AAD03282.1 transl_table: 1 translation: MNTLANIQELARALRNMIRTGIIVETDLNAGRCRVQTGGMCTDWLQWLTHRAGRSRTWWAPSVGEQVLILAVGGELDTAFVLPGIYSGDNPSPSVSADALHIRFPDGAVIEYEPETSALTVSGIKTASVTASGSVTATVPVVMVKASTRVTLDTPEVVCTNRLITGTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGSPL*"
     gene            19852..20199
                     /label="W"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: W"
     CDS             19852..20199
                     /label="P2-gpW"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GI: 3139102 experiment: experimental evidence, no additional detailsrecorded function: baseplate assembly protein gene: W product: gpW protein_id: AAD03283.1 transl_table: 1 translation: MTARYLGMNRSDGLTVTDLEHISQSIGDILRTPVGSRVMRRDYGSLLASMIDQPQTPALELQIKVACYMAVLKWEPRVTLSSVTTARSFDGRMTVTLTGQHNDTGQPLSLTIPVS*"
     gene            20204..21112
                     /label="J"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: J"
     CDS             20204..21112
                     /label="P2-gpJ"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GI: 3139103 experiment: experimental evidence, no additional detailsrecorded function: baseplate assembly protein gene: J product: gpJ protein_id: AAD03284.1 transl_table: 1 translation: MPIIDLNQLPAPDVVEELDFESILAERKATLISLYPEDQQEAVARTLTLESEPLVKLLEENAYRELIWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLTITPADDSTIPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISVTSPSPACVTISVLSRENNGVASEDLLAVVRNALNGEDVRPVADRVTVQSAAIVEYQINATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELAAPLADIVLNSTQASFCTEYRVVTGGSDE*"
     gene            21105..21635
                     /label="I"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: I"
     CDS             21105..21635
                     /label="P2-gpI"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GI: 3139104 experiment: experimental evidence, no additional detailsrecorded function: baseplate assembly protein gene: I product: gpI protein_id: AAD03285.1 transl_table: 1 translation: MSDSRLLPTGSSPLEVAAAKACAEIEKTPVSIRELWNPDTCPANLLPWLAWAFSVDRWDEKWPEATKRAVIRDAYFIHCHKGTIGAIRRVVEPLGYLINVTEWWENSDPPGTFRLDIGVLESGITEAMYQEMERLIADAKPASRHLIGLNITRDIPGYLFAGGVAYDGDVITVYPG*"
     CDS             21646..23655
                     /label="P2-gpH"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GI: 3139105 function: probable tail fiber protein gene: H product: gpH protein_id: AAD03286.1 transl_table: 1 translation: MSIKFRTVITTAGAAKLAAATAPGGRKVNITTMAVGDGGGKLPVPDAGQTGLIHEVWRHALNKISQDKRNSNYIIAELVIPPEVGGFWMRELGLYDDAGTLIAVANMAESYKPALAEGSGRSQTCRMVIIVSSVASVELTIDTTTVMATQDYVDDKIAEHEQSRRHPDASLTAKGFTQLSSATNSTSETLAATPKAVKAAYDLANGKYTAQDATTARKGLVQLSSATNSTSETLAATPKAVKTVMDETNKKAPLNSPALTGTPTTPTARQGTNNTQIANTAFVMAAIAALVDSSPDALNTLNELAAALGNDPNFATTMTNALAGKQPKDATLTALAGLATAADRFPYFTGNDVASLATLTKVGRDILAKSTVAAVIEYLGLQETVNRAGNAVQKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNDADGDTDSYMWFETGDNGNEYFKWRSRQSTTTKDLMTLKWDALNILVNAVINGCFGVGTTNALGGSSIVLGDNDTGFKQNGDGILDVYANSQRVFRFQNGVAIAFKNIQAGDSKKFSLSSSNTSTKNITFNLWGASTRPVVAELGDEAGWHFYSQRNTDNSVIFAVNGQMQPSNWGNFDSRYVKDVRLGTRVVQLMARGGRYEKAGHTITGLRIIGEVDGDDEAIFRPIQKYINGTWYNVAQV*"
     CDS             21646..23655
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MSIKFRTVITTAGAAKLAAATAPGGRKVNITTMAVGDGGGKLPVPDAGQTGLIHEVWRHALNKISQDKRNSNYIIAELVIPPEVGGFWMRELGLYDDAGTLIAVANMAESYKPALAEGSGRSQTCRMVIIVSSVASVELTIDTTTVMATQDYVDDKIAEHEQSRRHPDASLTAKGFTQLSSATNSTSETLAATPKAVKAAYDLANGKYTAQDATTARKGLVQLSSATNSTSETLAATPKAVKTVMDETNKKAPLNSPALTGTPTTPTARQGTNNTQIANTAFVMAAIAALVDSSPDALNTLNELAAALGNDPNFATTMTNALAGKQPKDATLTALAGLATAADRFPYFTGNDVASLATLTKVGRDILAKSTVAAVIEYLGLQETVNRAGNAVQKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNDADGDTDSYMWFETGDNGNEYFKWRSRQSTTTKDLMTLKWDALNILVNAVINGCFGVGTTNALGGSSIVLGDNDTGFKQNGDGILDVYANSQRVFRFQNGVAIAFKNIQAGDSKKFSLSSSNTSTKNITFNLWGASTRPVVAELGDEAGWHFYSQRNTDNSVIFAVNGQMQPSNWGNFDSRYVKDVRLGTRVVQLMARGGRYEKAGHTITGLRIIGEVDGDDEAIFRPIQKYINGTWYNVAQV*"
     primer          22299..22318
                     /label="gpH_fw"
                     /note="sequence: aggccttgtccagcttagta"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    22528..22617
                     /label="MutationRecombinationgpH"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(22779..22798)
                     /label="gpH_rv"
                     /note="sequence: ccgtttcctgtaaaccgaga"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             23659..24186
                     /label="P2-gpG"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GI: 3139106 function: probable tail fiber assembly protein gene: G product: gpG protein_id: AAD03287.1 transl_table: 1 translation: MQHLKNIKSGNPKTKEQYQLTKNFDVIWLWSEDGKNWYEEVKNFQPDTIKIVYDENNIIVAITRDASTLNPEGFSVVEVPDITSNRRADDSGKWMFKDGAVVKRIYTADEQQQQAESQKAALLSEAENVIQPLERAVRLNMATDEERARLESWERYSVLVSRVDPANPEWPEMPQ*"
     CDS             23659..24186
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MQHLKNIKSGNPKTKEQYQLTKNFDVIWLWSEDGKNWYEEVKNFQPDTIKIVYDENNIIVAITRDASTLNPEGFSVVEVPDITSNRRADDSGKWMFKDGAVVKRIYTADEQQQQAESQKAALLSEAENVIQPLERAVRLNMATDEERARLESWERYSVLVSRVDPANPEWPEMPQ*"
     primer          23741..23762
                     /label="gpG_fw"
                     /note="sequence: ggttatggtccgaagacggaaa"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    23869..23958
                     /label="MutationRecombinationPlacegpG"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(24020..24039)
                     /label="gpG_rv"
                     /note="sequence: ttccgcttcggaaagtaacg"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     variation       24206..26145
                     /label="del2 deletion"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: del2 deletion"
     regulatory      24551..24578
                     /label="putative rho-independent terminator for V operon"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: putative rho-independent terminator for V operon regulatory_class: terminator: "
     CDS             complement(24587..26173)
                     /label="P2-fun(Z)"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GI: 3139107 function: nonessential; lysogenic conversion; conferssensitivity to FUdR gene: fun(Z) note: cloned gene blocks growth of T5 product: Fun(Z) protein_id: AAD03288.1 transl_table: 1 translation: MIPIKDLTLGYADAENYKRRENKELLNKVFIRDNHLSRLCEPNISFLVGEKGTGKTAYSIYLSNNNINNTLATTKYIRETEYQKFITLKSEKHLNLSDFSGIWKVILYLLISKQVLDKEGKISKLLNYSKFSSLNDAIDEYYLKAFSPEIIQALSFVQESKVAAELLHKHATLKGEERESLSFSESRFQINLFYIQKKFEEAFSQIRLSQNHILFIDGIDIRPSSIPYDDYLECIKGLAHAVWEINNDFFPSIKGGKGRMKAVLLIRPDIFESIGLQNQNAKIRDNSVFLDWRTEYVNHRSSQLFEVLDHLLRTQQDMALKKGQAWDHYFPWDSPNVHDNYNHPTSFINFLRWSYYRPRDILRMLEIIKSHASGDDGKNQFSLDDFESPSMQRDYSNYLLGEIKDHLSFYYGSEHYEIFLKFFEFLSGKDTFDYAEYLLAYKQLETHISSISTTKPKFMTTANDFLQFLFGLNVICYIETTEDNNRFIRWCFRERSYANISPKIKTGMKYQIFYGLAKSLNVGKALKK*"
     gene            complement(24587..26173)
                     /label="fun(Z)"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: fun(Z)"
     variation       25179..26211
                     /label="del5 deletion"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: del5 deletion"
     promoter        26447..26537
                     /label="P2-PF promoter"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_fwdcolor="#800000"
                     /note="note: PF promoter from P2 phageThis is the PF promoter taken from the P2 phage genome. It is an inducible promoter that is activated by a class of activators, including P2 ogr (I746350), PSP3 pag (I746351) and phiR73 delta (I746352). These different activators should cause different levels of activity of the PF promoter.Part:BBa_I746360"
     misc_feature    26467..26495
                     /label="miscellaneous"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="experiment: experimental evidence, no additional detailsrecorded note: activator binding site; activation of pF promoter"
     CDS             26564..27754
                     /label="P2-gpFI"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GI: 3139108 function: essential tail protein; tail sheath gene: FI product: gpFI protein_id: AAD03289.1 transl_table: 1 Mutation: L120M, the phenotypic result of this mutation is unknown. translation: MSDYHHGVQVLEINEGTRVISTVSTAIVGMVCTASDADAETFPLNKPVLITNVQSAISKAGKKGTLAASLQAIADQSKPVTVVMRVEDGTGDDEETKLAQTVSNIIGTTDENGQYTGLKALLAAESVTGVKPRILGVPGLDTKEVAVALASVCQKLRAFGYISAWGCKTISEVKAYRQNFSQRELMVIWPDFLAWDTVTSTTATAYATARALGLRAKIDQEQGWHKTLSNVGVNGVTGISASVFWDLQESGTDADLLNESGVTTLIRRDGFRFWGNRTCSDDPLFLFENYTRTAQVLADTMAEAHMWAVDKPITATLIRDIVDGINAKFRELKTNGYIVDATCWFSEESNDAETLKAGKLYIDYDYTPVPPLENLTLRQRITDKYLANLVTSVNSN*"
     CDS             26564..27754
                     /label="gpFI"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MSDYHHGVQVLEINEGTRVISTVSTAIVGMVCTASDADAETFPLNKPVLITNVQSAISKAGKKGTLAASLQAIADQSKPVTVVMRVEDGTGDDEETKLAQTVSNIIGTTDENGQYTGLKAMLAAESVTGVKPRILGVPGLDTKEVAVALASVCQKLRAFGYISAWGCKTISEVKAYRQNFSQRELMVIWPDFLAWDTVTSTTATAYATARALGLRAKIDQEQGWHKTLSNVGVNGVTGISASVFWDLQESGTDADLLNESGVTTLIRRDGFRFWGNRTCSDDPLFLFENYTRTAQVLADTMAEAHMWAVDKPITATLIRDIVDGINAKFRELKTNGYIVDATCWFSEESNDAETLKAGKLYIDYDYTPVPPLENLTLRQRITDKYLANLVTSVNSN*"
     gene            27767..28285
                     /label="FII"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: FII"
     CDS             27767..28285
                     /label="P2-gpFII"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GI: 3139109 function: essential tail protein; tail tube gene: FII product: gpFII protein_id: AAD03290.1 transl_table: 1 translation: MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMNGSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATGVDAVPIRFAGSYQRDDTGETVAVEVVMRGRQKEIDTGEGKQGEDTESKISVVCTYFRLTMDGKELVEIDTINMIEKVNGVDRLEQHRRNIGL*"
     CDS             28342..28596
                     /label="P2-gpE+E'"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GI: 3139111 function: essential tail protein gene: E+E' note: shares amino terminus with gpE; -1 frameshift product: gpE+E' protein_id: AAD03292.1 transl_table: 1 translation: MNKENVITLDNPVKRGEQVIEQVTLMKPSAGTLRGVSLAAVANSEVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFL,VAELGAVTFPKNLSVDDLMADVAVIFHWPPSELYPMSLTELITWREKALRRSGNTNE*"
     gene            28342..28617
                     /label="E"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: E"
     CDS             28342..28617
                     /label="P2-gpE"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GI: 3139110 function: essential tail protein gene: E product: gpE protein_id: AAD03291.1 transl_table: 1 translation: MNKENVITLDNPVKRGEQVIEQVTLMKPSAGTLRGVSLAAVANSEVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ*"
     gene            28342..28769
                     /label="E+E'"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: E+E'"
     CDS             28596..28769
                     /label="P2-gpE+E'"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GI: 3139111 function: essential tail protein gene: E+E' note: shares amino terminus with gpE; -1 frameshift product: gpE+E' protein_id: AAD03292.1 transl_table: 1 translation: MNKENVITLDNPVKRGEQVIEQVTLMKPSAGTLRGVSLAAVANSEVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFL,VAELGAVTFPKNLSVDDLMADVAVIFHWPPSELYPMSLTELITWREKALRRSGNTNE*"
     CDS             28762..31209
                     /label="P2-gpT"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GI: 3139112 function: essential tail protein; putative tail length determinator gene: T product: gpT protein_id: AAD03293.1 transl_table: 1 translation: MSNNVKLQVLLRAVDQASRPFKSIRTASKSLSGDIRETQKSLRELNGHASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKRAAEDLQAKYNRLTDSVKRQQRELAAVGINTRNLAHDEQGLKNRISETTAQLNRQRDALVRVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAGTMAGVKLLMPGYEFAQKNSELQAVIGVAKDSAEMAALRKQARQLGDNTAASADDAAGAQIIIAKAGGDVDAIQAATPVTLNMALANRRTMEENAALLMGMKSAFQLSNDKVAHIGDVLSMTMNKTAADFDGMSDALTYAAPVAKNAGVSIEETAAMVGALHDAKITGSMAGTGSRAVLSRLQAPTGKAWDALKELGVKTSDSKGNTRPIFTILKEMQASFEKNRLGTAQQAEYMKTIFGEEASSAAAVLMTAASTGKLDKLTAAFKASDGKTAELVNIMQDNLGGDFKAFQSAYEAVGTDLFDQQEGALRKLTQTATKYVLKLDGWIQKNKSLASTIGIIAGGALALTGIIGAIGLVAWPVITGINAIIAAAGAMGAVFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFGGVVEGLKAAFAPVGELFTPLKPVFDWLGEKLQAAWQWFKNLIAPVKATQDTLNRCRDTGVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTHTATYGTGDYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGTAPGTQLDRQLQDALEKYERDKRARARASMMHDG*"
     gene            28762..31209
                     /label="T"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: T"
     RBS             31207..31215
                     /label="Shine-Dalgarno sequence"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: full consensus sequence for ribosome-binding sites upstream of start codons in E. coli; complementary to a region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             31224..31703
                     /label="P2-gpU"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GI: 3139113 function: essential tail protein gene: U product: gpU protein_id: AAD03294.1 transl_table: 1 translation: MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAFQFLGVGEENITLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISRVSETGSIFFADGTPRKIDFTLSLTRVDESLAALYGDIGKQAESLIGKAGSMATRFTGMTGAG*"
     gene            31224..31703
                     /label="U"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: U"
     CDS             31703..32866
                     /label="P2-gpD"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GI: 3139114 function: essential tail protein gene: D product: gpD protein_id: AAD03295.1 transl_table: 1 translation: MLDALTFDAGSTLTPDYMLMLDSRDITGNISDRLMSMTLTDNRGFEADQLDIELNDADGQVGLPVRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVTIRARSADFRGTLNSRREGSWHDTTLGAIVKAIATRNRLEASVAPSLAGIKIPHIDQSQESDAKFLTRLAERNGGEVSVKMGKLLFLKAGQGVTASGKKIPQVTITRSDGDRHHFAIADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVRQKKAPKVPEAREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSISLATGRADIYTETPVKVSGFKRVIDEQDWTITKVTHFLNNSGFTTSLELEVRLSDVEYETEDDE*"
     gene            31703..32866
                     /label="D"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: D"
     prim_transcript 32920..33200
                     /label="putative"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: putative"
     gene            32948..33166
                     /label="ogr"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: ogr"
     CDS             32948..33166
                     /label="P2-ogr"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 db_xref: GI: 3139115 gene: ogr product: Ogr protein_id: AAD03296.1 transl_table: 1 translation: MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYESVQRYIVKPGEVHAVRPHPLPSGQQIMWM*"
     regulatory      33177..33200
                     /label="P2 ogr terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="experiment: experimental evidence, no additional detailsrecorded note: rho-independent terminator for ogr transcript and F operon regulatory_class: terminator: "
     protein_bind    33205..33232
                     /label="Int binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: Int note: P arm"
     protein_bind    33236..33269
                     /label="IHF binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: IHF"
ORIGIN
        1 ggagatttag ggtgtcacca gtaggggctt tcaacggtac aatgcgggtt tgagcggcat
       61 aaattaccac tgaaagccct taaacgttac tctactgtgg acactgtgtg gacactctcg
      121 gcctcagtac cacctcttag cggattaaga gaaatggcgt cctgaaggta ctctggcgca
      181 aaatgagcgt aaaccatagt ttgctcaatc cgcgtgtgac ctagtatccg ttgtagcgtg
      241 ataatacttc ctccattaat catgaaatga gtggcaaagc tgtgccttag tgcatgtgtg
      301 gcttgcccca ttggcaaatc cggttttatt gctttcattg ttcgtctgaa gcgagggtaa
      361 tcagcatcag ggaataaaaa acctcgtttg ttatccgcga tcattttggc aacagcctct
      421 gagatcggga cggtgcgtgg tttgtttgtt ttcgttttaa caaacgtgac gcggttatgg
      481 atgatatttt ctgctttcaa acgagctgct tctccccaac gtgctcctgt actcaggcaa
      541 agaatcgcaa tctttttatt gtcgccgtca agtgctgcaa gcagtaaggc aatttcttcc
      601 tgtgttagat agcctgtttc tggtttttcc tccttaagcc tttttgtccc tctgataggg
      661 tgctcaccaa agaataactc cgcttcaatc agggctgtaa acatgccgct aatacatgtt
      721 aaatcacgat tgatactcga aggtttaata ccctgacttc ttcgggtggc gcagtactgg
      781 ctgataagcg atttcgtaat ttgaaatgcg catgggtcat tcgttatttt tgtgaagatt
      841 tcaatttttc caagattaga tttcccatgc tcttcgtgtt taccctttaa atcccaccag
      901 atctgtgtca gctccgacag acgtcgcttg tctgttggtt ttgatagcca ttctttattg
      961 tggtggttgt acaacgtgta tttctcgaaa gcgacagctt cgcttttctt atcaaacttc
     1021 ctacggatgc gttttccatt acgtccagta gggcggatgt ccacttcata tcgaccatca
     1081 tcgagttttt tgattgccat cagaaaaccc tccgagtggt gtgttttttt gcgactacta
     1141 atcgcttttt tcgtggtggc tgaaatttag ccaccaatag taggcacttg tgatgaatat
     1201 attcacgatg aattgttaac cagtcttttg accggagtgg ggcgacgttg tttcgttttg
     1261 cccaaagtgt gcgagagcgg gcgcaatttg cccggactca ggagcgatct gattggtcat
     1321 gaaccataaa gtgtatttgg tgaattgtgg ggtctgcagg atgttcatca tgacatctgt
     1381 tggaggtgtt gaacgaccac tttcatagta actcagcgtg ccatacggaa cccctgttaa
     1441 atcagcaagt tgttgtctgc tcaaatactc tgattttcgc attaagacta tcttctcgct
     1501 tatcgtgttt gacatggtgt ttagatctca atagtattta gtttagatgt agattgttta
     1561 gtgcttggat gtgggcacta aaaggcatta taagacatta aacgcaattc atgagggcta
     1621 gaggacgaca tgagcaagca agtaacactc atgactgatg cgattcctta tcaggagttc
     1681 gcaaaactaa taggaaaatc gacaggagcg gttcgtcgga tgatcgataa aggaaagctg
     1741 cctgtaattg atatgaccga tccacaatca gcttcaggtc gtgcaggtga atattgggta
     1801 taccttccgg catggaataa cggactaaaa ctggcttatg aaagccgccc taaagagatt
     1861 cgtgacggct ggttgatgtg gttaggtctc ggtgaaccac gttaaggaga accgtatgaa
     1921 tgagcctcgt tgtattgctc agttactgcg taacgaaagc cccagggcga ttgacttcac
     1981 catcacccac ggtaaggggc gtaagggaat cattatccgc accaaaaaac agagtccgtt
     2041 aaaaaaggct ctgacctttc tgaaaagccg gagggtatgg aaatgacagt gatgacgctc
     2101 aatctcgttg aaaaacagcc agcagctatg cgccggataa ttggtaagca tcttgccgtt
     2161 cctcgctggc aggatacatg tgattattat aatcagatga tggagcgcga acggttaacg
     2221 gtttgcttcc atgctcagtt aaaacagcgt cacgcaacga tgtgttttga agaaatgaac
     2281 gacgtcgaac gtgaacgact ggtatgtgca attgatgaat tgcgtggtgc attctcaaaa
     2341 cgccgtcagg ttggcgcaag tgagtatgca tatattagtt ttttaacagt cagtcagcgt
     2401 cgtactttat ttatgcatgc cggattgact gaaaaagaat tcaaccagcc atactggcga
     2461 attaatgaag agtcatgtta ctggcgtgat gctttattcc gtgcattacg tgaattattc
     2521 agtctgtttg agtatgcacc gacaattctg acgtcggtaa aaccggagca atatctgcat
     2581 taagtaatta accagagttt ttaacgcact taattgtgcg gggcttcttt ttgcctggag
     2641 aaagttatgc atacagtttc tgaaaatcag tgcggtatat acgcattact gctgcaacag
     2701 gccagaaccg aagcacaggc cgacgctgcg acgcgctttt cttctcatct tgatgccatg
     2761 attcgccaca tcacaaaggc ggagttatcc cgcgtggaga tagtcgagct gctcagtcag
     2821 gagtcggaaa aatttcacaa tatcggattg tctcgcgggg aggtgctttg atgttctgtt
     2881 ctcgtgcagt tgtattactg aataacgcct taaaaatcgc cgttatgaaa aatggcgatt
     2941 tgtctcttat tcaacttggt cttgataaag aaaaacgcga aataactgaa tctgttatcg
     3001 cgatttatca gagtgaatta aacctcctgt ctgatgtggt caatttactt gttaaacgcg
     3061 ctgtatttca caagcaaatt tcctccgtgg atgaactgac gaaattaacg acagaaatcg
     3121 ccagctattg cgctgatgaa tttaaaaaac tgaacgacaa aaggaactgg taatgccgga
     3181 caacgtagat tttattcagg aacaacaggc tgaattactg gagcgccaga ttaacgcggc
     3241 aagggtaaaa cattgcggtg cttctgcgct ggtttgcgaa gagtgtgacg cgccaatacc
     3301 tgctgcccgt cgtgcggctt acccgtcagc cacgcgttgt gtttcctgtc agtcagtctt
     3361 tgaagcaaaa aacaaacatt accggagaac ggcatgagta ttcgtattga aattggcgaa
     3421 cgttatgtcg ttaccagtga cagctttcag tttattctcc acgagaaaaa gagagcggaa
     3481 agcggtaaaa acgccggtca ggaatggctg gcggtggttg gttattaccc gaaattaagc
     3541 cagctcgttt caggcctgat gcatcacgat attctgaccg gaagcgcaaa gtcttttgct
     3601 gatttaaacg tgcaggttga gcaactcagc aagcgttgtt cagaggcttt tggctcatat
     3661 ggccgttaaa gcctccgggc gttttgtccc tccgtcagca tttgccgcag gcaccggtaa
     3721 gatgtttacc ggtgcttatg catggaacgc gccacgcgag gccgtcgggc gcgaaagacc
     3781 ccttacacgt gacgagatgc gtcagatgca aggtgtttta tccacgatta accgcctgcc
     3841 ttactttttg cgctcgctgt ttacttcacg ctatgactac atccggcgca ataaaagccc
     3901 ggtgcacggg ttttatttcc tcacatccac ttttcagcgt cgtttatggc cgcgcattga
     3961 gcgtgtgaat cagcgccatg aaatgaacac cgacgcgtcg ttgctgtttc tggcagagcg
     4021 tgaccactat gcgcgcctgc cgggaatgaa tgacaaggag ctgaaaaagt ttgccgcccg
     4081 tatctcatcg cagcttttca tgatgtatga ggaactcagc gatgcctggg tggatgcaca
     4141 tggcgaaaaa gaatcgctgt ttacggatga ggcgcaggct cacctctatg gtcatgttgc
     4201 tggcgctgca cgtgctttca atatttcccc gctttactgg aaaaaatacc gtaaaggaca
     4261 gatgaccacg aggcaggcat attctgccat tgcccgtctg tttaacgatg agtggtggac
     4321 tcatcagctc aaaggccagc gtatgcgctg gcatgaggcg ttactgattg ctgtcgggga
     4381 ggtgaataaa gaccgttctc cttatgccag taaacatgcc attcgtgatg tgcgtgcacg
     4441 ccgccaggca aatctggaat ttcttaaatc gtgtgacctt gaaaacaggg aaaccggcga
     4501 gcgcatcgac cttatcagta aggtgatggg cagtatttct aatcctgaaa ttcgccggat
     4561 ggagctgatg aacaccattg ccggtattga gcgttacgcc gccgcagagg gtgatgtggg
     4621 gatgtttatc acgcttaccg cgccttcaaa gtatcacccg acacgtcagg tcggaaaagg
     4681 cgaaagtaaa accgtccagc taaatcacgg ctggaacgat gaggcattta atccaaagga
     4741 tgcgcagcgt tatctctgcc atatctggag cctgatgcgc acggcattca aagataatga
     4801 tttacaggtc tacggtttgc gtgtcgtcga gccacaccac gacggaacgc cgcactggca
     4861 tatgatgctt ttttgtaatc cacgccagcg taaccagatt atcgaaatca tgcgtcgcta
     4921 tgcgctcaaa gaggatggcg acgaaagagg agccgcgcga aaccgttttc aggcaaaaca
     4981 ccttaaccag ggcggtgctg cggggtatat cgcgaaatac atctcaaaaa acatcgatgg
     5041 ctatgcactg gatggtcagc tcgataacga taccggcaga ccgctgaaag acactgctgc
     5101 ggctgttacc gcatgggcgt caacgtggcg catcccacaa tttaaaacgg ttggtctgcc
     5161 gacaatgggg gcttaccgtg aactacgcaa attgcctcgc ggcgtcagca ttgctgatga
     5221 gtttgacgag cgcgtcgagg ctgcacgcgc cgccgcagac agtggtgatt ttgcgttgta
     5281 tatcagcgcg cagggtgggg caaatgtccc gcgcgattgt cagactgtca gggtcgcccg
     5341 tagtccgtcg gatgaagtta acgagtacga ggaagaagtc gagagagtgg tcggcattta
     5401 cgcgccgcat ctcggcgcgc gtcatattca tatcaccaga acgacggact ggcgcattgt
     5461 gccgaaagtt ccggtcgttg agcctctgac tttaaaaagc ggcatcgccg cgcctcggag
     5521 tcctgtcaat aactgtggaa agctcaccgg tggtgatact tcgttaccgg ctcccacacc
     5581 ttctgagcac gccgcagcag tgcttaatct ggttgatgac ggtgttattg aatggaatga
     5641 accggaggtc gtgagggcgc tcaggggcgc attaaaatac gacatgagaa cgccaaaccg
     5701 tcagcaaaga aacggaagcc cgttaaaacc gcatgaaatt gcaccatctg ccagactgac
     5761 caggtctgaa cgattgcaga tcacccgtat ccgcgttgac cttgctcaga acggtatcag
     5821 gcctcagcga tgggaacttg aggcgctggc gcgtggagca accgtaaatt atgacgggaa
     5881 aaaattcacg tatccggtcg ctgatgagtg gccgggattc tcaacagtaa tggagtggac
     5941 atgatggcaa aaattcacga ggtaaagctg cacgcaaaat atttcgacct tgtactggaa
     6001 ggaaagaaac gcgcagagtt tcggaaaaaa gatcgtaatt atgagcgcgg ggacacgttg
     6061 attttgcatg aatgggtgca gggtgtgttt acggggcgaa aggttgaagc ccggataaca
     6121 gatgttactg acctgtcaga ctggctggaa gattatgtct tgctgcacag aatactaggt
     6181 gagatactgc cgctgactgt caagctggca gtatcctcca cattttttat aaatctaagg
     6241 cgaagaacgg tggcaatata tttgctttaa attgtaaata agcaaattat tctaagccac
     6301 aaatgatatc aattgagtat cgattttcgt tcgaatataa ccataacgcc aagcgtcaaa
     6361 tgtaagtcat ctatatgtga ttcgtattca tattggtcag catcaggaga tagtaaatgc
     6421 tctgctgctg taaaaacatc aattgtaaca gatatatcgt ctggaatttc ttcatttaat
     6481 tcggatatgt attttttata tttgtttttt aatttcctta tcatgtcttc cttatggtgg
     6541 attgtagtgc agtaattttt tttagttgaa tcattgtatg aatggaatga acagtttaat
     6601 gctaggccaa gacttgaatt tcctgtaggg cctgtgtgtt taaagtattc aagggtgcat
     6661 gagattgcat taagggaaac tgtgatatgg cttggctttt gactggaaat actgatggct
     6721 cattagtttt atCTCGGTAC CAAAGACGAA CAATAAGACG CTGAAAAGCG TCTTTTTTCG
     6781 TTTTGGTCCg gccacaattc agcaaattgt gaacatcatc acgttcatct ttccctggtt
     6841 gccaatggcc cattttcctg tcagtaacga gaaggtcgcg aattAaggcg ctttttagac
     6901 tggtcgtgAC TAGAGAAAGA GGAGAAATAC TAGatgattt actgtccgtc gtgtggacat
     6961 gttgctcaca cccgtcgcgc acatttcatg gacgatggca ccaagataat gattgcacag
     7021 tgccggaata tttattgctc tgcgacattt gaagcgagtg aaagcttttt ctctgacagt
     7081 aaagattcag gaatggaata catttcaggc aaacagagat accgcgattc actgacgtca
     7141 gcctcctgcg gtatgaaacg cccgaaaaga atgcttgtta ccggatattg ttgtcggaga
     7201 tgtaaaggcc ttgcactgtc aagaacatcg cggcgtctgt ctcaggaagt caccgagcgt
     7261 ttttatgtgt gcacggatcc gggctgtggt ctggtgttta aaacgcttca gaccatcaac
     7321 cgcttcattg tccgcccggt cacgccggac gaactggcag aacgcctgca tgaaaaacag
     7381 gaactgccgc cagtacggtt aaaaacacaa tcatattcgc tgcgtctgga ataataaCgt
     7441 cAcAAAGAGG GGAAAatcTA cATGCGTAAC AAAAAAGCAC CGCAGACCGT TTCCGCGCGT
     7501 CACGACGCGC GCGAACACCT GTCCATCGAA GCGTATCACA AACTGAACCG TGCCAGCGCC
     7561 GTCAGCCGCT TCGTTGGCGG CGACCcGATC CACCGTGAAC TGAGCGGTCT GCACCAGCTG
     7621 TAtATCCCGC ACATCTTCTC CTACCTGAAC GAAGAcATCG ACTTCGTGCT GAATGAGCTG
     7681 AAAGCGAAAG GCCTGTGCCG TGATTTCCTG GCGCAGCAGA AAGACCGCGG CGATCGCACC
     7741 CACGTTtaat aacgcaaaaa ccccgcttca gcggggtttt ttcgcccgaa gttcctattc
     7801 tctagaaagt ataggaactt cttgacagct agctcagtcc taggtactgt gctagcaagt
     7861 gaggaactaa accatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat
     7921 cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc
     7981 tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg
     8041 tttctacaaa gatcgttatg tttatcgAca ctttgcatcg gccgcgctcc cgattccgga
     8101 agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca
     8161 gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc
     8221 ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt
     8281 cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga
     8341 tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca
     8401 ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca
     8461 cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga
     8521 ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag
     8581 gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct
     8641 tgcaggatcg ccgcggctcc ggAcgtatat gctccgcatt ggtcttgacc aactctatca
     8701 gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat
     8761 cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt
     8821 ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccggatgagg
     8881 ggatcaattc tctagagctc gctgatCCAA TTATTGAAGG CCGCTAACGC GGCCTTTTTT
     8941 TGTTTCTGGT CTCCCcagaa gttcctattc tctagaaagt ataggaactt cttagtctgc
     9001 ccgttgaggc gttgtgtgtc tgcggggtgt tttgtgcggt ggtgagcgtg tgagggggga
     9061 tgacggggtg taaaaaagcc gcccgcaggc ggcgatgttc agtcgttgtc agtgtccagt
     9121 gagtagtttt taaagcggat gacctcctga ccgagccagc cgtttatctc gcggatcctg
     9181 tcctgtaacg ggataagctc attgcggaca aagacctttg ccactttctc aatatcaccc
     9241 agcgacccga cgttctccgg cttgccaccc atcaactgaa aggggatgcg gtgcgcgtcc
     9301 agcaggtcag cggcgctggc ttttttgata ttaaaaaaat cgtccttcgt cgccacttca
     9361 ctgaggggga taattttaat gccgtcggct ttcccctgtg gggcatagag aaacaggttt
     9421 ttaaagttgt tgcggccttt cgacttgacc atgttttcgc gaagcatttc gatatcgttg
     9481 cgatcctgca cggcatcggt gacatacatg atgtatccgg catgtgcgcc attttcgtaa
     9541 tacttgcggc ggaacaacgt ggccgactca ttcagccagg cagagttaag ggcgctgaga
     9601 tattccggca ggccgtacag ctcctgatta atatccggct ccagcaggtg aaacacggag
     9661 ccgggcgcga aggctgtcgg ctcgttgaag gacggcaccc accagtaaac atcctcttcc
     9721 acgccacggc gggtatattt tgccggtgag gtttccagtc tgatgacctt accggtggtg
     9781 ctgtaacgct tttccagaaa cgcattaccg aacaccagaa aatccagcac aaagcggctg
     9841 aaatcctgct gggaaagcca tggatgcggg ataaatgtcg aggccagaat attgcgtttg
     9901 acgtaaatcg gcgagctgtg atgcacggca gcccgcaggc tttttgccag accggtaaag
     9961 ctgaccggtg gctcatacca tctgccgtta ctgatgcact cgacgtaatc cagaatgtca
    10021 cggcggtcga gtaccggcac cggctcacca aaggtgaatg cctccatttt cgggccgctg
    10081 gcggtcattg tttttgccgc aggttgcggt gttttccctt ttttcttgct catcagtaaa
    10141 actccagaat ggtggatgtc agcggggtgc tgataccggc ggtgagtggc tcatttaaca
    10201 gggcgtgcat ggtcgcccag gcgaggtcgg cgtggctggc ttcctcgctg cggctggcct
    10261 cataggtggc gctgcgtccg ctgctggtca tggtcttgcg gatagccata aacgagctgg
    10321 tgatgtcggt ggcgctgacg tcgtattcca gacagccacg gcggataacg tcttttgcct
    10381 tgagcaccat tgcggttttc atttccggcg tgtagcggat atcacgcgcg gcgggataga
    10441 acgagcgcac gagctggaac acgccgacac cgaggccggt ggcatcaata ccgatgtatt
    10501 cgacgttgta tttttcggtg agtttgcgga tggattccgc ctgggtggca aagtccatgc
    10561 ctttccactg gtgacgctca agtattctga atttgccacc ggccaccacc ggcggtgcca
    10621 gtaccacgca tccggcgctg tcgccacggt gtgacgggtc gtaaccaatc cataccgggc
    10681 gggagccgaa cggattggcg gcaaacggcg catagtcttc ccattcttcc agcgtgtcga
    10741 ccatgcagcg ttgcagctcc tcgaacggga acaccgatgc cttgtcgtca acaaattcac
    10801 acatgaacag gtttttaaaa tcgtcggcgc tgttttcgcg tttgagctgc tcaatgtcga
    10861 acagcgtgca gccgcctttc agggcgtcct caatggtgac aatctgtcgc cactggccgt
    10921 ccgcacagag aagcccaccg gcaagggcgt tatgactgac gtcgatttcc acgcgttcgg
    10981 cggcgctggc gcgtccccgg ttaaacagtt cacccgacca gaacgggtag gcgtcgtgcg
    11041 ccagcgtgga cggggtggag aaataggtcg agcgcaggtg actctgtgag gccatacctg
    11101 atgccacctt acgcagtacc tgaaaattcg ggatccagaa aatctcatcg acgtacaggt
    11161 cgccgttatg actctgcgcg gtgttggagt tggtgccgag aaaaatcagt tttgcgccgt
    11221 tattgcccag gacaatcggg tcaccggtca ggtcaacgtc aaccagacgg gcaaaggcga
    11281 tgatgtattc gcggaacaca tacgcctgtg ttttactggc cgacagaaaa atctggttat
    11341 gaccggtttt cagggcacgc agcagcgcct cgcgggaaaa ataaaacgtc gcgccaatct
    11401 ggcgggattt caggatatcg cggatgcggt gctcaagccc ggcacgatac cagtgcagct
    11461 gatagtcgaa agactgctca aagaaaatct gctccagctt ttcgatggcc tcgtcactga
    11521 aaaaattctt tttcggtttg cgccgtccgc ctttgttacg gttagcgacg ttcggattaa
    11581 ggtctgcctc gttgccggtc tggctgtagc ggttgacccg tgccagtcgt tcaatctggc
    11641 gtcccagcag gtcaatttcc ttgaagtcac cgccggtttt ctgtggtttg atgatgagct
    11701 gggtcagccg cgcttccaga ctcatttcga cacggctgat gggggcaacg ctgtcccagc
    11761 cgtcgcgctg tttccagctc tgcactgtcg ggcgtttcat ctgcaacatg gcggcaatct
    11821 gcggcacgga aaacccctgc cagtacagca gcgccgcctg acgacgcggg tcgtgtaaaa
    11881 gagtggtgtc tgtggtgatg gtcatgaata cctcgccgtg atgaatacac ggcaaggcta
    11941 ctgagtcgcg ccccgcgatt cgctaaggtg ctgttgtgtc agtgataagc catccgggac
    12001 tgatggcgga ggatgcgcat cgtcgggaaa ctgatgccga catgtgactc ctctaatcac
    12061 tattcaggac tcctgacaat ggcaaaaaaa gtctcaaaat tctttcgtat cggcgttgag
    12121 ggtgacacct gtgacgggcg tgtcatcagt gcgcaggata ttcaggaaat ggccgaaacc
    12181 tttgacccgc gtgtctatgg ttgccgcatt aacctggaac atctgcgcgg catcctgcct
    12241 gacggtattt ttaagcgtta tggcgatgtg gccgaactga aggccgaaaa gattgacgat
    12301 gattcggcgc tgaaaggcaa atgggcgctg tttgcgaaaa tcaccccgac cgatgacctt
    12361 atcgcgatga acaaggccgc gcagaaggtc tacacctcaa tggaaattca gccgaacttt
    12421 gccaacaccg gcaaatgtta tctggtgggt ctggccgtca ccgatgaccc ggcaagcctc
    12481 ggcacggaat acctggaatt ctgccgcacg gcaaaacaca accccctgaa ccgcttcaaa
    12541 ttaagccctg aaaacctgat ttcagtggca acgcctgttg agctggaatt tgaagacctg
    12601 cctgaaaccg tgttcaccgc cctgaccgaa aaggtgaagt ccatttttgg ccgcaaacag
    12661 gccagcgatg atgcccgtct gaatgacgtg catgaagcgg tgaccgctgt tgctgaacat
    12721 gtgcaggaaa aactgagcgc cactgagcag cgcctcgctg agatggaaac cgccttttct
    12781 gcacttaagc aggaggtgac tgacagggcg gatgaaacca gccaggcatt cacccgcctg
    12841 aaaaacagtc tcgaccacac cgaaagtctg acccagcagc gccgcagcaa agccaccggc
    12901 ggtggcggtg acgccctgat gacgaactgc tgaccggcgt cagtcagtcc gggaaaacct
    12961 tcacgattaa cccttaattt caggaaaaac tatgcgccag gaaacccgct ttaaatttaa
    13021 tgcctacctg tcccgtgttg ccgaactgaa cggcatcgac gccggtgatg tgtcgaaaaa
    13081 attcaccgtt gaaccgtcgg tcacccagac cctgatgaac accatgcagg agtcctctga
    13141 ctttctgacc cgcatcaata ttgtgccggt cagcgaaatg aaaggggaaa aaattggtat
    13201 cggtgtcacc ggctccatcg ccagcactac cgacactgcc ggtggtaccg agcgtcagcc
    13261 gaaggacttc tcgaagctgg cgtcaaacaa gtacgaatgc gaccagatta acttcgattt
    13321 ttatatccgc tacaaaacgc tggacctgtg ggcgcgttat caggatttcc agctccgtat
    13381 ccgtaacgcc attatcaaac gccagtccct tgatttcatc atggccggtt ttaacggcgt
    13441 gaagcgtgcc gaaacctctg accgcagcag caatccgatg ttgcaggatg tggcggtcgg
    13501 ctggctgcag aaataccgca atgaagcacc ggcgcgcgtg atgagcaagg tcactgacga
    13561 ggaaggccgc accacctctg aggttatccg cgtgggtaag ggcggtgatt atgccagcct
    13621 tgatgcactg gtgatggatg cgaccaacaa cctgattgaa ccgtggtatc aggaagaccc
    13681 tgaccttgtg gtgattgtgg ggcgtcagct actggcggac aagtatttcc ccatcgtcaa
    13741 caaggagcag gacaacagcg aaatgctggc cgctgacgtc atcatcagcc agaaacgcat
    13801 cggtaaccta ccagcggtac gcgtcccgta cttcccggcg gatgcgatgc tcatcacgaa
    13861 gctggaaaac ctgtccatct actacatgga tgacagccat cgccgcgtga ttgaggaaaa
    13921 cccgaaactc gaccgcgtgg agaactacga gtcaatgaac attgattacg tggtggaaga
    13981 ctacgccgcc ggttgtctgg tggaaaaaat caaggtcggt gacttctcca caccggctaa
    14041 ggcgaccgca gagccgggag cgtaaccgat gacgagtccc gcacagcgcc acatgatgcg
    14101 ggtctcggca gcgatgaccg cgcagcggga agccgccccg ctgcgacatg caactgtcta
    14161 tgagcagatg ctggttaagc tcgccgcaga ccagcgcaca ctgaaagcga tttactcaaa
    14221 agagctgaag gccgcaaaaa aacgcgaact gctgccgttc tggttgccgt gggtgaacgg
    14281 cgtgctggag ctgggcaaag gtgcacagga tgacattctg atgacggtca tgctgtggcg
    14341 tctggatacc ggcgatattg ccggtgcgct ggagattgcc cgttatgccc tgaagtacgg
    14401 tctgaccatg ccgggtaaac accgccgtac cccgccgtac atgttcaccg aggaggtagc
    14461 gcttgcggcc atgcgcgctc acgctgccgg tgagtctgtg gatacccgcc tgctgacgga
    14521 gacccttgaa ctgaccgcca cggctgacat gcctgatgaa gtgcgcgcaa agctgcacaa
    14581 aatcaccggt ctgtttctgc gtgacggtgg tgatgccgcc ggtgcgctgg cgcacctgca
    14641 acgtgcgaca cagctcgact gtcaggcagg cgtcaaaaaa gagattgaac gactggagcg
    14701 ggagctgaaa ccgaagccgg agccgcagcc caaagcggcc acccgcgccc cgcgtaagac
    14761 ccggagcgtg acaccggcaa aacgtggacg cccgaaaaag aaagccagtt aacaaccgaa
    14821 tgcgccccgc gccagggcgg cacgccggtc agtgacggtg aatcacctga cactgcaccg
    14881 gcgtccaccg cccgactttt cagaggtagt catgatgacg ctgattattc cgcgaaagga
    14941 ggctcccgtg tccggtgagg gtacggtggt catcccgcaa ccggcaggcg acgagccggt
    15001 gattaaaaac acgttctttt ttcccgatat cgacccgaag cgcgtccggg aacgtatgcg
    15061 ccttgagcag accgtcgccc ccgcccgtct gcgtgaggcc atcaagtcag gcatggctga
    15121 aacgaatgcg gagctgtacg agtaccgcga acagaaaatt gccgccggtt ttacgcgtct
    15181 ggctgacgtc ccggcggacg atatcgacgg tgaaagcatc aaggtttttt actacgagcg
    15241 cgccgtgtgt gcgatggcga ccgcgtcgct ttatgagcgt tatcgcggtg tggatgccag
    15301 tgcgaaaggc gacaagaagg ctgacagcat tgacagcacc attgatgagc tgtggcggga
    15361 tatgcgctgg gcggtggcgc gcatccaggg caagccgcgc tgcatcgtga gtcaaatctg
    15421 atgaagacct ttgcgctaca gggcgacacg ctcgacgcca tttgtgtccg ctattacggg
    15481 cgcactgagg gcgtggttga gaccgtgctc gccgcaaatc cgggactggc tgaactgggg
    15541 gcggtgctgc cacacggcac cgccgtcgaa ctgcccgacg ttcagaccgc gcccgtggct
    15601 gaaactgtca atctgtggga gtaacgcatg acagcagaag aaaaaagcgt cctgtcgctt
    15661 ttcatgattg gggtgctgat tgttgtcggc aaggtgcttg ccggtggtga acctatcacc
    15721 ccgcgtctgt ttatcgggcg catgttgctc ggtggttttg tctcgatggt tgccggtgtt
    15781 gttctggtgc agtttcctga cctgtcactg ccagcggtgt gcggcatcgg ctccatgctg
    15841 ggtatcgccg gttatcaggt gattgagatt gccattcagc gccgctttaa gggcaggggg
    15901 aaacagtaat gccggtaatt aacacgcatc agaatatcgc cgcctttctc gacatgctgg
    15961 ccgtgtccga agggacggcg aatcatccac tgacgaaaaa ccggggctat gacgtgatag
    16021 tcaccggact ggacgggaag ccggaaattt tcaccgacta cagtgaccac ccgttcgcac
    16081 atggccgacc ggcgaaggtg tttaaccgtc gcggtgaaaa atccacggcc tccggtcgct
    16141 atcagcagct ttacctgttc tggccgcatt accgcaaaca gcttgccctg ccggatttca
    16201 gtccgttgtc acaggacaga ctcgccattc agttgatccg cgaacgcgga gcactggatg
    16261 acatccgggc gggacgcatt gagcgcgcca tttcacgctg tcgcaatatc tgggcgtccc
    16321 tgccgggtgc cggttacggt cagcgtgagc attcactgga aaaactggtc accgtctggc
    16381 gtaccgctgg cggcgtaccg gcttaaacgg agtaaatacc atgaagaaat tatccctttc
    16441 actgatgctg aacgtgtcgc tggcgctgat gctggcactg tccctgattt acccgcagag
    16501 cgtggccgtc aattttgtcg ctgcctgggc gattctggcg acggttatct gtgtggttgc
    16561 cggtggtgtg ggcgtgtatg ccactgagta tgtgctggaa cgctacgggc gggagctgcc
    16621 gccggaatcg ctggccgtga agattgtcac gtcgctgttt ttgcagccgg tgccgtggcg
    16681 cagacgggcg gcggctctgg tagtggtggt ggcgacgttt atctcgctgg tcgctgccgg
    16741 gtggattttt accgcgctga tttatcttgt ggtgtcgctg tttttccggc tgatacgtaa
    16801 agcctgtcgt cagcgtcttg aggggcggga accatgtcaa ggctgatgat tgtgctggtc
    16861 gtgttgttat cgctggcggt ggccggtctg tttctggtga aacacaaaaa tgccagcctg
    16921 cgcgcctcgc tggacagggc gaacaacgtc gccagcggtc agcagacgac catcaccatg
    16981 ctgaaaaatc agcttcatgt tgcgctcacc agggcagata aaaacgagct ggcgcaggtg
    17041 gcactgcgtc aggaactgga gaacgccgcg aaacgtgaag cacagcgcga gaaaaccatc
    17101 acgaggttac ttaatgagaa cgaagatttt cgccgctggt acggtgctga cctgcctgat
    17161 gctgtgcgcc ggttgcacca gcgccccgcc tgcaccgacg ccagtgattg tccccaacgc
    17221 atgcccgaaa gtgagccttt gcccgatgcc gggcagtgac ccgcagacga acggcgattt
    17281 aagtgccgat atccggcagc ttgagaacgc gctggcacgc tgtgccagcc aggtaaaaat
    17341 gattaaacac tgtcaggacg aaaacgatgc tcaaacccga cagcctgcgc agggcgctga
    17401 ctgatgccgt cacggtgctg aaaactaacc ccgatatgct gcggatattc gtggataacg
    17461 ggagtattgc ctccacactg gcggcgtcgc tgtcattcga aaagcgttac acgctcaatg
    17521 tgattgtgac cgactttacc ggtgattttg acctgctcat tgtgccggtg ctggcgtggc
    17581 tgcgggaaaa tcagcccgac atcatgacca ccgacgaagg ccagaaaaag ggcttcacgt
    17641 tttatgcaga catcaacaat gacagcagct ttgatatcag tatcagcctg atgctgaccg
    17701 agcgcacgct ggtcagtgag gtggacggcg cactgcatgt gaagaatatc tcggaacccc
    17761 cgccgccgga gccggtcacc cgcccgatgg agctgtatat caatggcgaa ctggtgagta
    17821 agtgggatga atgagtttaa gcgttttgaa gaccggctga ccggactgat tgaatcgctg
    17881 tcaccgtcag ggcgtcggcg actgagtgcc gaactggcga aacgtctgcg gcagagtcag
    17941 cagcgtcggg tgatggcaca gaaagccccg gacggcacac cctacgcgcc acgccagcag
    18001 cagagcgtca gaaaaaagac cggtcgcgtt aagcgaaaaa tgtttgcgaa acttattacc
    18061 agtcgttttt tgcatatccg tgccagcccg gagcaggcat caatggaatt ttacggcggg
    18121 aagtcgccga aaatcgccag tgtgcatcag tttggtctgt cggaagaaaa ccggaaagac
    18181 ggtaagaaaa ttgattatcc ggcgcgtccc ctgctcggct ttaccggtga ggatgtgcag
    18241 atgattgaag agattatcct ggctcacctt gagcgttagt tttatccagg cagaggctga
    18301 tgcgcaatta aacattgagc ggccatgctg gtcgctcaat gtttagaggt ttatgagtga
    18361 tttttatttg atgctttgta ttctaaaacc ttcttattgg cgtaaaagaa ttttgtatat
    18421 gacaggaata taaccagacc tgaagtgaaa tagacgaggg atagtattaa taatgctttt
    18481 ttgtgactgt tattatcttt aatctcctgg cttaaccatt cggagtcctc ctcgtttagc
    18541 tgtaagagct tattgcaggc gatctcagga agtgtgtctt ttataaatac gttttgcagt
    18601 ctcttgcaat cggcaaggct ataagtttta ttaaattcaa ctgctttatt tttgaaggat
    18661 aaaagaactt tgtcactata aacatagtac atcatgtttt tatatggtat gcctgtggca
    18721 tcccttacta taacggattg ttcgttgtgt atgtaacatg cgaggagaat gtaaaaaata
    18781 ctggccagaa ttacaattat tgttttaatt atgtgtggtg gttttgttat gtcaccccag
    18841 atacgagtaa ggaaaaaata cgatgttttt agttttccat caatcagtcc ctgctgtatc
    18901 attctcacat tttcaatgcc tgatacattg attccgttaa ttattttaaa tagttgaatg
    18961 tcgcgccact cgcggtcaag tctttttaat tttttgtctg aatatccaaa attgaaataa
    19021 tgtgcaataa gcctcataag gttactttta ccaaagctaa aaaatgctaa tactgcaaag
    19081 ctacaaagga aaaaaacgat tagcccccac acattagtca cattatagct gaccattacg
    19141 ctctccttga atgttgtctg gtagttctac aaatgaatcc agatagcata acttttatat
    19201 attgtgcaat ctcacatgca tgaacactct cgcaaatatt caggaactcg cgcgcgcact
    19261 gcgcaacatg attcgcactg gcattatcgt cgaaaccgac cttaacgccg gtcgctgccg
    19321 cgtgcagacc ggcggcatgt gcaccgactg gcttcagtgg ctgacccatc gcgcaggacg
    19381 ttcgcgcaca tggtgggcac cttccgtggg ggaacaggtg ctgattctgg ccgtgggtgg
    19441 tgaactcgac acggcgttcg ttctgccggg gatttattcc ggcgataacc cctcgccgtc
    19501 tgtgtcggcg gatgccctgc atatccgttt ccctgacggg gcggtgattg aatatgaacc
    19561 cgaaaccagt gcactcacgg taagcggaat taaaacggcc agcgtgacgg cttccggttc
    19621 tgttactgcc acggtgccgg tggtcatggt gaaagcatca acccgcgtca ccctggacac
    19681 cccggaggtg gtctgcacca acaggctgat taccggcacg ctggaagtgc agaaaggcgg
    19741 gacgatgcgc ggcaacattg aacacaccgg cggtgaactc tcatcaaacg gtaaggtact
    19801 gcatacccat aaacaccccg gcgacagcgg cggcacaacc gggagtcctt tatgacagcg
    19861 cgttatctcg gaatgaatcg cagtgatggc ctgactgtca ctgaccttga gcatatcagc
    19921 cagagtatcg gcgatatcct gcgcacaccg gtcggctcac gggtgatgcg tcgtgattac
    19981 ggctcgttgc tggcgtcaat gattgaccag ccgcagaccc cggcgcttga gttgcagatt
    20041 aaagtcgcct gttacatggc agtgctgaaa tgggaacccc gcgtcaccct gtcatccgtc
    20101 accacggcgc gcagttttga cgggcgaatg acggtcacgt taaccggcca gcacaacgac
    20161 accggccagc cactttcatt aaccatccct gtgagttgaa accatgccga ttatcgacct
    20221 gaaccagcta cccgcaccgg atgtggtcga ggagctggac tttgaaagca ttctcgctga
    20281 acgcaaggcg acactgattt ccctttaccc ggaagatcag caggaggcgg tcgcccgtac
    20341 cctgacactg gaatctgagc ctctcgtcaa actgctggaa gaaaatgctt atcgtgagct
    20401 tatctggcgt cagcgtgtga atgaggccgc acgggcggtg atgctggcct gtgccgccgg
    20461 taatgacctt gatgtgattg gtgccaatta caacaccacg cgcctgacta tcaccccggc
    20521 agatgattcg accatcccgc cgacaccggc agtgatggaa tctgacaccg attatcgtct
    20581 gcgtattcag caggcttttg agggcttaag cgtcgccggg tcagtgggag cctatcagta
    20641 tcatggtcgc agtgctgacg ggcgtgtcgc ggatatttct gtcaccagtc cgtctccggc
    20701 ctgtgtcacc atctctgtgc tgtcacgtga aaataacggc gtcgcatccg aagacctgct
    20761 ggctgtggtg cgtaacgccc ttaatggcga ggacgtcagg ccggtggccg accgcgtgac
    20821 cgtgcagtct gccgccatcg ttgaatacca gataaacgcc acgctttacc tttaccctgg
    20881 tcccgaaagc gaacccatcc gcgctgccgc tgtgaaaaag ctggaagcgt atatcacggc
    20941 acagcaccgg ctggggcgcg acatccgtct gtctgccatt tatgccgctt tgcatgtgga
    21001 aggtgtgcag cgtgtcgaac tggctgcacc actggccgac atcgtgctca acagtacgca
    21061 ggcgtctttc tgtaccgaat accgcgtcgt gaccggaggc tcggatgagt gattcgcgac
    21121 tgctgccgac cggctcatca ccgcttgagg tcgccgccgc aaaagcctgt gcggaaattg
    21181 aaaaaacgcc ggtcagtatt cgtgaactgt ggaacccgga cacctgtccg gcaaatctgc
    21241 tgccgtggct ggcgtgggcg ttttcggtcg acaggtggga tgaaaagtgg ccggaagcga
    21301 caaaacgcgc cgttatccgc gatgcctatt tcatccactg tcataagggc acgataggtg
    21361 caatccggcg tgtggtggag ccgctcggct atctcatcaa cgtgacggag tggtgggaaa
    21421 acagtgaccc gcccggcacc ttccggcttg atattggtgt actggaaagc ggtatcacag
    21481 aggcaatgta tcaggaaatg gaacggctga ttgctgatgc caaacctgca agccgtcacc
    21541 ttattggcct gaacattacc cgggacattc ccggctatct gttcgccggt ggtgtggctt
    21601 acgacggcga tgtaattacg gtttaccccg gataagtgag gaataatgag cataaaattc
    21661 agaaccgtta tcaccactgc cggtgcagca aagctggcag cggcaaccgc gccgggaggg
    21721 cggaaggtca acattaccac gatggccgtc ggggatggcg gtggtaaatt gcctgtcccg
    21781 gatgccggac agaccgggct tatccatgaa gtctggcgac atgcgctgaa caaaatcagc
    21841 caggacaaac gaaacagtaa ttatattatc gccgagctgg ttattccgcc ggaggtgggc
    21901 ggtttctgga tgcgtgagct tggcctgtac gatgatgcgg gaacgttaat tgccgtggcg
    21961 aacatggccg aaagctataa gccagccctt gccgaaggct caggacgttc gcagacctgt
    22021 cgcatggtca tcatcgtcag cagtgtggcc tcagtggagc tgaccattga caccacaacg
    22081 gtgatggcga cgcaggatta cgttgatgac aaaattgcag agcacgaaca gtcacgacgt
    22141 cacccggacg cctcgctgac agcaaaaggt tttactcagt taagcagtgc gaccaacagc
    22201 acgtctgaaa cactggccgc aacgccgaaa gcggtaaagg ccgcgtatga cctggctaac
    22261 gggaaatata ccgcacagga cgccaccaca gcgcgaaaag gccttgtcca gcttagtagc
    22321 gccaccaaca gcacgtctga aacgctcgcc gcaacaccaa aagccgttaa gacggtaatg
    22381 gatgaaacga acaaaaaagc gccattaaac agccctgcac tgaccggaac gccaacgacg
    22441 ccaactgcgc gacagggaac gaataatact cagatcgcaa acacggcttt cgttatggcc
    22501 gcgattgccg cccttgtaga ctcgtcgcct gacgcactga atacgctgaa cgagctggcg
    22561 gcggcgCTGg gcaatGACcc gaattttgct accaccatga ctaatgcgct tgcgggtaag
    22621 caaccgaaag atgctaccct gacggcgctg gcggggcttg ctactgcggc agacaggttt
    22681 ccgtatttta cggggaatga tgttgccagc ctggcgaccc tgacaaaagt cgggcgggat
    22741 attctggcta aatcgaccgt tgccgccgtt atcgaatatc tcggtttaca ggaaacggta
    22801 aaccgagccg ggaacgccgt gcaaaaaaat ggcgatacct tgtccggtgg acttactttt
    22861 gaaaacgact caatccttgc ctggattcga aatactgact gggcgaagat tggatttaaa
    22921 aatgatgccg atggtgacac tgattcatac atgtggtttg aaacggggga taacggcaat
    22981 gaatatttca aatggagaag ccgccagagt accacaacaa aagacctgat gacgttgaaa
    23041 tgggatgcac taaatattct tgttaatgcc gtcattaatg gctgttttgg agttggtacg
    23101 acgaatgcac taggtggtag ctctattgtt cttggtgata atgataccgg atttaaacag
    23161 aatggagacg gtattcttga tgtttatgct aacagtcagc gtgtattccg ttttcagaat
    23221 ggagtggcta ttgcttttaa aaatattcag gcaggtgata gtaaaaagtt ctcgctatcc
    23281 agctctaata catccacgaa gaatattacc tttaatttat ggggtgcttc cacccgtcca
    23341 gtggttgcag agttaggcga tgaggccgga tggcatttct atagccagcg aaatacagat
    23401 aactcggtaa tatttgctgt taacggtcag atgcaaccca gcaactgggg aaattttgat
    23461 tcccgctatg tgaaagatgt tcgcctgggt acgcgagttg ttcaattgat ggcgcgaggt
    23521 ggtcgttatg aaaaagccgg acacacgatt accggattaa gaatcattgg tgaagtagat
    23581 ggcgatgatg aagccatctt caggccgata caaaaataca tcaatggcac atggtataac
    23641 gttgcgcagg tgtaagttat gcagcattta aagaacatta agtcaggtaa tccaaaaaca
    23701 aaagagcaat atcagctaac aaagaatttt gatgttatct ggttatggtc cgaagacgga
    23761 aaaaactggt atgaggaagt gaagaacttt cagccagaca caataaagat tgtttacgat
    23821 gaaaataata ttattgtcgc tatcaccaga gatgcttcaa cgcttaatcc tgaaggtttt
    23881 agcgttgttg aggttcctga tATTacctcc aacCGAcgtg ctgacgactc aggtaaatgg
    23941 atgtttaagg atggtgctgt ggttaaacgg atttatacgg cagatgaaca gcaacaacag
    24001 gcagaatcac aaaaggccgc gttactttcc gaagcggaaa acgttattca gccactggaa
    24061 cgcgctgtca ggctgaatat ggcgacggat gaggaacgtg cacgactgga gtcatgggaa
    24121 cgttacagcg ttctggtcag ccgtgtggat cctgcaaatc ctgaatggcc ggaaatgccg
    24181 caataagttg tatgacctct gttgtgaact tacatatcta tggcacagag taaagcctaa
    24241 tctgaagtcc actctgtgcc aaaagcggac cttataaaca agaacaatgg tcatatcagt
    24301 gtgttttgat tgcatagcta acgtgcgtct tcctgtacag aatcataaga tgatagggca
    24361 taggagatga ttattatcgc gtgttttaaa aatagctttc tgcatcaagt gtcacttgcg
    24421 aagaggtatt ggcgctatga ttggcatcac tcagttcaga taatatttta aatatttggt
    24481 aaatgttcag attacacaat tcggtctgcg tcccgttaaa attatctact attagttatt
    24541 actatgaggt gaatggcaag tgttttccac tcgccatttt tgttttttat ttttttaaag
    24601 cctttccgac gtttaaggat tttgccaagc cataaaatat ttgatatttc attcctgttt
    24661 ttatttttgg tgagatattt gcgtaacttc tttctctaaa acaccaacgt ataaacctat
    24721 tattatcttc ggttgtttct atatagcata ttacattgag accaaaaaga aactgaagga
    24781 agtcatttgc agttgtcatg aattttggtt ttgttgtgga aatggaacta atatgagttt
    24841 caagttgttt atatgctaag aggtattcag cgtaatcaaa agtatctttt ccagagagga
    24901 attcaaagaa ctttaaaaaa atctcataat gttcacttcc ataataaaat gataaatgat
    24961 cttttatttc tccaagtaaa tagttagagt aatctctttg catgctagga ctttcaaagt
    25021 catctagact aaattgattt ttcccatcat caccgctggc gtgtgatttg ataatttcga
    25081 gcatccgtaa tatatctctt gggcgataat atgaccagcg aaggaaatta ataaatgatg
    25141 tggggtggtt ataattatca tggacgttgg gtgaatccca agggaaataa tggtcccatg
    25201 cctgtccctt tttaagggcc atgtcttgct gagtcctaag cagatgatct aatacctcaa
    25261 ataattgaga gctacggtgg ttaacatatt ctgtccgcca gtccaagaat actgaattat
    25321 ctctgatttt tgcgttttga ttttgtaatc ctatggattc aaatatatct ggcctgatca
    25381 agagaacggc cttcattctc ccttttcctc cctttatact tgggaagaaa tcgttgttaa
    25441 tctcccagac tgcgtgagct aatcctttta tacattctag ataatcatcg tatggaattg
    25501 aggaagggcg gatatcaata ccatcgataa ataaaatatg attttgagat aaacgtattt
    25561 ggctaaaggc ctcttcaaat tttttttgta tataaaataa atttatctga aatctgctct
    25621 cagaaaaaga taaagactct ctttcttcac cttttaaggt tgcatgctta tgaagtagtt
    25681 cggctgcaac ttttgactct tgaacaaaac tgagggcttg aattatttct ggtgaaaatg
    25741 ccttaagata atactcatct attgcatcat taagtgatga gaattttgaa taatttagta
    25801 actttgaaat tttcccttct ttatctaata cttgttttga tattaataaa tatagaataa
    25861 ctttccatat gccagagaag tcagacaaat ttaaatgctt ttcagattta agagtgataa
    25921 atttttgata ttctgtctct cttatatatt tagttgtagc tagcgtattg ttaatattgt
    25981 tatttgagag atagatagag tatgcggttt taccagtacc tttctctcct acaagaaacg
    26041 atatatttgg ttcacataat ctagataggt gattatcacg tatgaacact ttgttaagta
    26101 attctttatt ctcccttctc ttataatttt cagcatcagc atagccaaga gttagatctt
    26161 tgattggtat cattattacc tcaactaaag caattgaaaa acatatggtt ataaaaacat
    26221 agcgctgctc tgtcgcatat tgtggatatc aacatatgaa atgtaaagga actatttgtg
    26281 gatgaatgaa ccgaatcgct gtagcttcgg tagtcatctt tcagacttgt ataaatgaac
    26341 aacttccgct tctcgctcaa aggaaactgt cagatttgat agcttttggg ctatgtaaac
    26401 tgtcagtcgg aaaatgagtg agtacaaatc agggcaggtg agcgaattgc ccgccttttc
    26461 tttaccggtg gttgtgctgt cgattagcca accgggacaa atagcctgac atctccggcg
    26521 caactgaaaa taccactcac ccattaacca cggagttaaa cggatgagtg actatcatca
    26581 cggcgtgcag gtgctggaga ttaacgaggg cacccgcgtc atttccaccg tatccacggc
    26641 cattgtcggc atggtctgca cggccagcga tgcagatgcg gaaaccttcc ccctcaataa
    26701 acctgtgctg attaccaatg tgcagagcgc aatttcaaag gccggtaaaa aaggcacgct
    26761 ggcggcatcg ttgcaggcca tcgctgacca gtcaaaaccg gtcaccgttg tcatgcgcgt
    26821 ggaagacggc accggtgatg acgaggaaac gaaactcgcg cagaccgttt ccaatatcat
    26881 cggcaccacc gatgaaaacg gtcagtacac cggactaaaa gccAtgctgg cggcggagtc
    26941 ggtaaccggt gttaaaccgc gtattctcgg cgtgccggga ctggatacca aagaggtggc
    27001 tgttgcactg gcatcagtct gtcagaagct gcgtgctttc gggtatatca gcgcatgggg
    27061 ctgtaaaacc atttccgagg tgaaagccta tcgtcagaat ttcagccagc gtgagctgat
    27121 ggtcatctgg ccggatttcc tcgcatggga tacggtcacc agtaccaccg ccaccgcgta
    27181 tgccaccgcc cgtgcgctgg ggctgcgcgc taaaatcgac caggagcagg gctggcataa
    27241 aacgctgtcc aatgtcgggg tgaacggtgt taccggcatc agcgcatctg tattctggga
    27301 tttgcaggag tccggcaccg atgctgacct gcttaacgag tcaggcgtca ctacgctgat
    27361 tcgccgcgac ggtttccgct tctggggtaa ccgtacctgc tctgatgacc cgctgttcct
    27421 ctttgaaaac tacacccgca ccgcgcaggt gctggccgac acgatggctg aggcgcacat
    27481 gtgggcggtg gacaagccca tcactgcaac gctgattcgc gacatcgttg acggcatcaa
    27541 tgccaaattc cgtgagctga aaacaaacgg ctatatcgtg gatgcgacct gctggttcag
    27601 cgaagaatcc aacgatgcgg aaaccctcaa ggccggaaaa ctgtatatcg actacgacta
    27661 tacaccggtg cctcctctcg aaaacctgac cctgcgccag cgtattaccg ataaatacct
    27721 ggcaaatctg gtcacctcgg ttaacagcaa ttaaggagcc tgaccgatgg caatgccgcg
    27781 caaactcaag ttaatgaacg tctttctgaa cggctacagc tatcagggcg ttgcaaagtc
    27841 cgtcacgctg ccaaaactga cccgtaagct cgaaaactat cgcggtgcgg ggatgaacgg
    27901 cagcgcaccg gtagacctcg gccttgatga cgatgcgctg tcaatggagt ggtcgctcgg
    27961 tggcttcccg gattcggtta tctgggagct ttacgccgca accggtgtgg atgccgtgcc
    28021 gattcgtttt gcaggctctt accagcgcga cgataccggc gaaacggtgg ccgtcgaagt
    28081 ggtcatgcgt ggacgtcaga aagaaatcga caccggcgag ggtaaacagg gagaagacac
    28141 tgagtcgaaa atctccgtgg tctgcaccta tttccggctg acgatggacg gtaaggagct
    28201 ggtcgaaatt gacaccatca acatgattga gaaggtgaac ggcgtcgatc ggctggagca
    28261 acaccgccgc aatatcggcc tgtgattttc atccggtcag cctggctggc cggttaaccc
    28321 tgattcagaa gtgagaaaac catgaacaaa gaaaatgtca ttaccctgga caatccggtc
    28381 aaacgtggtg agcaggttat cgaacaggtc acgctgatga aacccagtgc cgggacgcta
    28441 cgcggtgtca gtctggctgc ggttgcaaac tccgaagtcg atgcactgat taaggtgctg
    28501 ccgcgcatga cggcaccgat gctgaccgag caggaagtcg ccgcgctgga actgcctgac
    28561 cttgtggcgc tggccggtaa ggtggtcggt tttttgtcgc cgaactcggt gcagtgacgt
    28621 ttccgaaaaa tctctcggtc gatgacctga tggcggatgt ggcagtgata tttcactggc
    28681 cgccatcaga actgtatccc atgagcctga ccgaactcat cacatggcgc gaaaaggcgc
    28741 tccggcgaag cggaaacacg aatgagtaac aatgtaaaat tacaggtatt gctcagggct
    28801 gttgaccagg catcccgccc gtttaaatcc atccgcacag cgagcaagtc gctgtcgggg
    28861 gatatccggg aaacacaaaa atcactgcgc gagctgaacg gtcacgcatc ccgtattgag
    28921 ggattccgca agaccagtgc acagctcgcc gtgactggtc atgcacttga aaaggcacgg
    28981 caggaggccg aagcccttgc cacacagttt aaaaacaccg aacgtccgac ccgtgctcag
    29041 gcgaaagtcc tggaatccgc aaagcgtgcg gcggaggact tacaggcgaa atataaccgc
    29101 ctgacagatt ccgttaaacg ccagcagcgg gaactggccg ctgtgggaat taatacccgc
    29161 aatcttgcac atgatgagca gggactgaaa aaccgtatca gtgaaaccac cgcacagctt
    29221 aaccgtcagc gtgatgcgct ggtgcgtgtc agtgcgcaac aggcaaaact taacgcagta
    29281 aaacagcgtt atcaggccgg aaaggaactg gccggaaata tggcctcagt gggcgctgcc
    29341 ggtgtgggga ttgcggcggc gggaacgatg gccggtgtta agctactgat gcccggttat
    29401 gagtttgcgc agaaaaactc agaattacag gctgtgatcg gagtggcaaa agactccgcc
    29461 gaaatggccg cactccgcaa gcaggcgcgc cagctcggcg acaataccgc cgcctcggca
    29521 gatgatgcag ccggtgcgca gattattatt gcgaaagccg gtggggatgt tgatgccatt
    29581 caggcggcaa cgccggtcac gctgaacatg gcgctggcga accgtcgcac aatggaagaa
    29641 aacgccgccc tgctgatggg gatgaaatcc gcctttcagc tttcaaacga taaggtcgct
    29701 catatcgggg atgttctctc catgacgatg aacaaaaccg ccgccgattt tgacggcatg
    29761 agcgatgcgc tgacctatgc cgcacctgtg gcaaaaaatg ccggtgtcag cattgaagaa
    29821 accgccgcaa tggtcggggc gctgcatgat gcaaaaatca caggctcaat ggcggggacg
    29881 ggaagccgtg ccgtgttaag ccgcctgcag gcaccgacgg gaaaagcatg ggatgcactc
    29941 aaagagcttg gagtgaaaac ctcagacagc aaaggaaaca cccggccaat atttaccatt
    30001 ctgaaagaaa tgcaggccag ttttgagaaa aaccggctcg gtactgccca gcaggctgaa
    30061 tacatgaaaa ctattttcgg ggaggaggcc agctcagccg ctgccgtgct gatgactgcc
    30121 gcctcaaccg gaaagctgga caaactgacc gctgcgttta aagcctcaga cgggaagacc
    30181 gccgagctgg taaatatcat gcaggacaac ctaggcggtg actttaaagc gtttcagtcc
    30241 gcttatgagg cggtggggac tgacctgttt gaccagcagg aaggcgcgct gcgtaagctc
    30301 acgcagacgg ccacaaagta tgtgttaaaa ctcgacggct ggatacagaa aaacaaatca
    30361 ctggcgtcaa ccatcggcat cattgccggc ggtgcactgg cgcttactgg catcatcggt
    30421 gccattggcc tcgtagcctg gccggttatc accggcatca atgccatcat cgcggcagca
    30481 ggcgcaatgg gggcagtctt cacgacggtt ggcagtgctg ttatgaccgc catcggggct
    30541 attagctggc cggttgtggc cgtggtggct gccattgtcg ccggtgcgtt gcttatccgt
    30601 aaatactggg agcctgtcag cgcattcttt ggtggtgtgg ttgaagggct gaaagcggca
    30661 tttgcgccgg tgggggaact gttcacgcca cttaaaccgg tttttgactg gctgggcgaa
    30721 aagttacagg ccgcgtggca gtggtttaaa aacctgattg ccccggtcaa agccacccag
    30781 gacaccctga accgttgccg tgacacgggc gtcatgttcg ggcaggcact ggctgacgcg
    30841 ttgatgctgc cgcttaatgc gttcaacaaa ctgcgcagtg gtattgactg ggtactggaa
    30901 aaactcggtg ttatcaacaa agagtcagac acacttgacc agaccgccgc cagaactcat
    30961 accgccacgt atggtaccgg tgactatatt ccggcgacca gctcttatgc aggctatcag
    31021 gcttatcagc cggtcacggc accggctggc cgctcttatg tagaccagag taaaaacgaa
    31081 tatcacatca gcctgacggg ggggactgcg ccggggacac agcttgaccg ccagttacag
    31141 gatgcgctcg aaaaatacga gcgggataaa cgtgcgcgcg cccgtgccag catgatgcat
    31201 gacggttaag gaggtgacga aaaatgatgc tcgcgttagg tatgtttgtt tttatgcgcc
    31261 agacgctgcc acaccagacc atgcagcgtg aatcagatta tcgctggccg tcaaattccc
    31321 gtatcggtaa acgggatgcc tttcagtttc tcggtgtggg tgaggaaaac atcacgctgg
    31381 ccggtgtgct ttatcccgaa ctgaccggcg gcaagctgac gatgaccacg ctcaggctga
    31441 tggcagagga ggggcgggcg tggccgttgc tggatggcac cggcatgatt tacggcatgt
    31501 atgtcatcag cagggtgagt gaaacaggga gtattttctt tgcagacggc acaccccgga
    31561 aaattgattt tacgctgtca ctcacccgcg ttgatgaatc actggccgcg ctttatggcg
    31621 atatcggtaa acaggcggaa tcgctcatcg gtaaggccgg cagtatggcg accagattca
    31681 caggtatgac gggggcggga taatgctgga tgcgctgaca tttgatgcag gcagtacgct
    31741 gacgccggat tacatgctga tgctcgacag cagggatatt accggcaata tcagcgaccg
    31801 tctgatgagc atgaccctga cggataaccg gggctttgag gctgaccagc ttgatattga
    31861 actgaacgat gccgacgggc aggtcgggct gccggttcgt ggcgctgtcc tgacggtgta
    31921 tatcggctgg aaaggttttg ccctggtatg caaagggaaa tttaccgttg atgaggttga
    31981 acaccggggc gcaccggatg tagtcaccat ccgcgcccgg agtgcagatt ttcgcgggac
    32041 gctcaattcc cgccgggaag gctcctggca tgacaccacg ctcggtgcga ttgttaaggc
    32101 gatagccacc cgtaacaggc tggaagccag tgtcgctccg tcactggccg gaataaaaat
    32161 tccacacatc gaccagtcgc aggagtctga tgcgaaattc ctgacccgtc ttgcagaacg
    32221 caacggcggt gaggtgtcgg taaaaatggg aaaactgttg tttctcaaag cggggcaggg
    32281 agtgacggcc agcggtaaaa aaatcccgca ggtcaccata acccgcagcg acggcgaccg
    32341 ccatcatttt gcgattgctg accgtggagc ctacaccggt gtaacggcaa aatggctaca
    32401 cactaaagac ccgaagccgc aaaagcagaa ggtaaaactg aaacgcaaaa agaaagagaa
    32461 acacctgcgc gcactggagc acccgaaagc gaaaccggtc aggcagaaga aagcgcctaa
    32521 agtaccggaa gcgcgtgaag gtgaatacat ggccggtgag gctgacaacg tttttgccct
    32581 gaccacggta tatgccacga aagcgcaggc catgcgcgcc gctcaggcga agtgggataa
    32641 actgcaacgg ggcgttgcgg agttctctat cagcctggct accggtcggg cagatattta
    32701 cacggaaaca ccggtcaaag tgtctggctt taagcgcgtc atagacgagc aggactggac
    32761 aatcactaag gtgacacatt ttctgaataa tagcggcttc acgacgtcct tagagcttga
    32821 ggtcaggctt tctgatgtgg agtacgaaac agaagatgat gagtgatgtt tttgttttat
    32881 ctgtttgttt tgtaaggata aattaactaa aatggcacca tcaacaaaac cggaagaggt
    32941 gctcgcgatg tttcattgtc ctttatgcca gcatgccgca catgcgcgta caagtcgcta
    33001 tatcactgac acgacaaaag agcgttatca tcagtgccag aacgtgaatt gcagcgccac
    33061 gttcatcact tatgagtcgg tacagcgata catcgtgaag ccgggagaag tccacgccgt
    33121 aaggccgcac ccgttgccat cagggcagca aattatgtgg atgtaattac aaacagaaag
    33181 cccctcagtc gaggggcttt tttgtcgatg tggtcaatgt gtggacgtga ccagaaataa
    33241 atccttttat ttcaatttat tgtacgtaaa aaataagccc gtgtaag
//