LOCUS       RC00214-pcsb-35Sp-ccdB 11475 bp ds-DNA     circular     13-SEP-2023
DEFINITION  .
ACCESSION   RC00214-pcsb-35S-ccd
VERSION     RC00214-pcsb-35S-ccd
KEYWORDS    "RIPE ID:RC00214" "Alias" "Assembly level" "Plant selection"
            "Bacterial selection:Spectinomycin resistance 1"
            "Promoter(s):Pro-CaMV35SShort, Pro-AtuOcs_5U-AtuOcs" "Gene of
            interest (GOI):mNeonGreen, tdTomato" "Tags"
            "Terminator(s):Ter-CaMV35S(2), 3U+Ter-AtuOcs" "User" "Lab"
COMMENT     Imported using the Genbank importer. File name:
            RC00214-pcsb-35S-ccdB-mNeonGreen-Ocspro-tdTomato.gb ..
FEATURES             Location/Qualifiers
     homology        1..3
                     /label="GGT"
                     /note="color: #f7e8f7"
     misc_feature    4..6708
                     /label="From ec36460-pcsb-peven-2"
                     /note="source: ec36460-pcsb-peven-2 indicates_part: True color: white"
     misc_feature    5..8
                     /label="AATG fusion site"
                     /note="ApEinfo_revcolor: #c7b0e3"
     misc_feature    22..46
                     /label="RB"
                     /note="ApEinfo_revcolor: #b7e6d7"
     primer          complement(90..109)
                     /label="R(0232)"
                     /note="sequence: GGATAAACCTTTTCACGCCC"
     primer          complement(122..147)
                     /label="F(0229)"
                     /note="sequence: GAACCCTGTGGTTGGCATGCACATAC"
     primer          complement(164..185)
                     /label="TB0076"
                     /note="sequence: agcggaggggttggatcaaagt"
     primer          complement(169..190)
                     /label="TB0080"
                     /note="sequence: atagcagcggaggggttggatc"
     primer          252..273
                     /label="TB0125"
                     /note="sequence: cctaagttacgcgacaggctgc"
     misc_feature    complement(1108..2108)
                     /label="STA\region\from\pVS1\plasmid"
                     /note="ApEinfo_revcolor: #ff9ccd"
     primer          complement(1334..1355)
                     /label="TB0126"
                     /note="sequence: aaccttcatgcgttccccttgc"
     misc_feature    complement(1347..1976)
                     /label="pVS1 StaA"
                     /note="ApEinfo_revcolor: #85dae9"
     misc_feature    complement(1716..2108)
                     /label="STA\region\from\pVS1\plasmid"
                     /note="ApEinfo_revcolor: #c7b0e3"
     misc_feature    2405..3478
                     /label="pVS1 RepA"
                     /note="ApEinfo_revcolor: #d6b295"
     misc_feature    2702..3702
                     /label="pVS1-Rep\ori"
                     /note="ApEinfo_revcolor: #d59687"
     misc_feature    3544..3738
                     /label="pVS1 oriV"
                     /note="ApEinfo_revcolor: #c6c9d1"
     primer          complement(3743..3760)
                     /label="SEQ080"
                     /note="sequence: GGCTGTGCGCTGGCCAGA"
     primer          3768..3789
                     /label="SEQ079"
                     /note="sequence: TGCAAAAAGCGCCTACCCTTCG"
     misc_feature    complement(4112..4372)
                     /label="pBR322"
                     /note="ApEinfo_revcolor: #84b0dc"
     primer          complement(4204..4223)
                     /label="SEQ082"
                     /note="sequence: GCCTGATGCGGTATTTTCTC"
     primer          4231..4248
                     /label="SEQ081"
                     /note="sequence: CGCTTCCTCGCTCACTGA"
     primer          complement(4252..4269)
                     /label="F(0014)"
                     /note="sequence: cgaacgaccgagcgcagc"
     primer          complement(4312..4337)
                     /label="F(0015)"
                     /note="sequence: CGTTATCCCCTGATTCTGTGGATAAC"
     primer          complement(4345..4362)
                     /label="SEQ040"
                     /note="sequence: CCTTTTGCTCACATGTTC"
     primer          complement(4363..4384)
                     /label="TB0146"
                     /note="sequence: acggttcctggccttttgctgg"
     misc_feature    complement(4393..5007)
                     /label="rep\(pMB1)"
                     /note="ApEinfo_revcolor: #ff9ccd"
     misc_feature    complement(4408..4996)
                     /label="ori"
                     /note="ApEinfo_revcolor: #d6b295"
     primer          complement(4424..4445)
                     /label="pUC57mini-R1"
                     /note="sequence: TTTTGTGATGCTCGTCAGGGGG"
     primer          complement(4427..4446)
                     /label="pMS-R1"
                     /note="sequence: TTTTTGTGATGCTCGTCAGG"
     rep_origin      complement(4512..4792)
                     /label="pBR322\ori"
                     /note="ApEinfo_revcolor: #c6c9d1"
     primer          complement(4893..4912)
                     /label="SEQ059"
                     /note="sequence: agagctaccaactctttttc"
     CDS             complement(5084..6094)
                     /label="SpmR"
                     /note="ApEinfo_revcolor: #b7e6d7"
     CDS             complement(5084..6094)
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQARYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK*"
     misc_feature    complement(5084..6094)
                     /label="Spectinomycin resistance 1"
     promoter        complement(6095..6228)
                     /label="Spm\Promoter"
                     /note="ApEinfo_revcolor: #75c6a9"
     misc_feature    complement(6229..6656)
                     /label="STA\region\from\pVS1\plasmid"
                     /note="ApEinfo_revcolor: #c7b0e3"
     primer          6464..6485
                     /label="TB0029"
                     /note="sequence: tgctaccctccgcgagatcatc"
     primer          6504..6525
                     /label="SEQ-LOOP-F3"
                     /note="sequence: gcttagttgccgttcttccgaa"
     primer          6527..6544
                     /label="SEQ-PLOOP-F2"
                     /note="sequence: agcatcggtaacatgagc"
     primer          6612..6631
                     /label="SEQ-PLOOP-F1"
                     /note="sequence: gtggtgattttgtgccgagc"
     primer          6657..6680
                     /label="R(0230)"
                     /note="sequence: CTGGTGGCAGGATATATTGTGGTG"
     misc_feature    6661..6686
                     /label="LB"
                     /note="ApEinfo_revcolor: #ffef86"
     misc_feature    6704..6707
                     /label="TACT fusion site"
                     /note="ApEinfo_revcolor: #c7b0e3"
     homology        6709..6711
                     /label="ATG"
                     /note="color: #f7e8f7"
     misc_feature    6712..6921
                     /label="From ec36455-pck1-spacer"
                     /note="source: ec36455-pck1-spacer indicates_part: True color: white"
     misc_feature    6713..6716
                     /label="GGAG fusion site"
                     /note="ApEinfo_revcolor: #c7b0e3"
     misc_feature    6717..6916
                     /label="Odd-spacer"
                     /note="ApEinfo_revcolor: #d6b295"
     misc_feature    6917..6920
                     /label="CGCT fusion site"
                     /note="ApEinfo_revcolor: #c7b0e3"
     homology        6922..6924
                     /label="GCA"
                     /note="color: #f7e8f7"
     misc_feature    6925..8740
                     /label="From ec36682"
                     /note="source: ec36682 indicates_part: True color: white"
     homology        6926..6929
                     /label="GGAG"
     Promoter        6926..7355
                     /label="35S pro short (no BpiI and BsaI)"
     misc_feature    6926..7355
                     /label="Pro-CaMV35SShort"
     misc_feature    6930..7355
                     /label="From pich41388"
     primer          6934..6955
                     /label="TB110"
                     /note="sequence: ACATGGTGGAGCACGACACTCT"
     primer          6934..6955
                     /label="TB0045"
                     /note="sequence: ACATGGTGGAGCACGACACTCT"
     primer          complement(6934..6955)
                     /label="TB0102"
                     /note="sequence: agagtgtcgtgctccaccatgt"
     primer          7177..7198
                     /label="TB0139"
                     /note="sequence: aagatggacccccacccacgag"
     primer          7230..7248
                     /label="SEQ041"
                     /note="note: sequence: acgtctacaaagcaagtgg"
     primer          7230..7248
                     /label="SEQ041"
                     /note="sequence: acgtctacaaagcaagtgg"
     primer          complement(7275..7296)
                     /label="TB0025"
                     /note="sequence: agtgggattgtgcgtcatccct"
     primer          7283..7305
                     /label="SEQ_PU:35S_F1"
                     /note="note: sequence: cgcacaatcccactatccttcgc"
     primer          7283..7305
                     /label="SEQ_PU:35S_F1"
                     /note="sequence: cgcacaatcccactatccttcgc"
     5'_UTR          7349..7355
                     /label="CaMV 35s 5'UT"
     5'_UTR          7349..7355
                     /label="CaMV 35s 5'UT"
     homology        7356..7359
                     /label="TACT"
     misc_feature    7360..7813
                     /label="From l-1m-5u-spacer-ccdb"
     misc_feature    7368..7805
                     /label="ccdB cassette"
     CDS             7537..7749
                     /label="ccdB"
     CDS             7537..7749
                     /translation="MVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI*"
     homology        7814..7817
                     /label="AATG"
     primer          7815..7836
                     /label="TB0081"
                     /note="sequence: atgGTGAGCAAAGGGGAGGAGG"
     misc_feature    7815..8525
                     /label="mNeonGreen"
     misc_feature    7818..8525
                     /label="From ec36252-pl0m-sc-mneongreen"
     primer          7847..7868
                     /label="mNeongreen 42-63 FWD 2"
                     /note="sequence: ATCACTGCCTGCAACACATGAG"
     primer          7847..7868
                     /label="mNeongreen 42-63 FWD 2"
                     /note="sequence: ATCACTGCCTGCAACACATGAG"
     primer          7898..7919
                     /label="mNeongreen 93-114 FWD 3"
                     /note="sequence: TGATTTTGACATGGTCGGGCAG"
     primer          7898..7919
                     /label="mNeongreen 93-114 FWD 3"
                     /note="sequence: TGATTTTGACATGGTCGGGCAG"
     primer          7963..7982
                     /label="mNeonGreen_F1"
                     /note="note: sequence: AGAGCACAAAGGGGGATCTT"
     primer          7963..7982
                     /label="mNeonGreen_F1"
                     /note="sequence: AGAGCACAAAGGGGGATCTT"
     primer          7963..7982
                     /label="mNeonGreen_F1"
                     /note="sequence: AGAGCACAAAGGGGGATCTT"
     primer          complement(7963..7984)
                     /label="mNeongreen 158-179 REV 2"
                     /note="sequence: TGAAGATCCCCCTTTGTGCTCT"
     primer          complement(7963..7984)
                     /label="mNeongreen 158-179 REV 2"
                     /note="sequence: TGAAGATCCCCCTTTGTGCTCT"
     primer          7982..8001
                     /label="mNeonGreen_F2"
     primer          7982..8001
                     /label="mNeonGreen_F2"
                     /note="sequence: TCAATTCAGCCCCTGGATAC"
     primer          7982..8001
                     /label="mNeonGreen_F2"
                     /note="sequence: TCAATTCAGCCCCTGGATAC"
     primer          complement(7984..8005)
                     /label="mNeongreen 179-200 REV 3"
                     /note="sequence: ACGAGTATCCAGGGGCTGAATT"
     primer          complement(7984..8005)
                     /label="mNeongreen 179-200 REV 3"
                     /note="sequence: ACGAGTATCCAGGGGCTGAATT"
     primer          8249..8270
                     /label="mNeongreen 444-465 FWD 1"
                     /note="sequence: CGCAGATTGGTGCCGATCTAAA"
     primer          8249..8270
                     /label="mNeongreen 444-465 FWD 1"
                     /note="sequence: CGCAGATTGGTGCCGATCTAAA"
     primer          complement(8340..8361)
                     /label="mNeongreen 535-556 REV 1"
                     /note="sequence: TCCTTGCCGTTGATCTGTACCT"
     primer          complement(8340..8361)
                     /label="mNeongreen 535-556 REV 1"
                     /note="sequence: TCCTTGCCGTTGATCTGTACCT"
     primer          8379..8398
                     /label="mNeonGreen_F3"
                     /note="note: sequence: AAGCCGATGGCAGCTAACTA"
     primer          8379..8398
                     /label="mNeonGreen_F3"
                     /note="sequence: AAGCCGATGGCAGCTAACTA"
     primer          8379..8398
                     /label="mNeonGreen_F3"
                     /note="sequence: AAGCCGATGGCAGCTAACTA"
     primer          complement(8472..8491)
                     /label="mNeonGreen_R1"
     primer          complement(8472..8491)
                     /label="mNeonGreen_R1"
                     /note="sequence: GTGAAAGCCTTCTGCCACTC"
     primer          complement(8472..8491)
                     /label="mNeonGreen_R1"
                     /note="sequence: GTGAAAGCCTTCTGCCACTC"
     primer          complement(8489..8510)
                     /label="TB0082"
                     /note="sequence: GTCCATGCCCATCACATCCGTG"
     homology        8526..8529
                     /label="GCTT"
     misc_feature    8530..8735
                     /label="From ec27347-pl0m-t-35s"
     misc_feature    8530..8735
                     /label="From ec27347-pl0m-t-35s"
     terminator      8530..8735
                     /label="35s Terminator polyA"
     misc_feature    8530..8735
                     /label="Ter-CaMV35S(2)"
     homology        8736..8739
                     /label="CGCT"
     homology        8741..8743
                     /label="TAC"
                     /note="color: #f7e8f7"
     misc_feature    8744..11262
                     /label="From ec36795-pck3-ocspro-tt-ocster"
                     /note="source: ec36795-pck3-ocspro-tt-ocster indicates_part: True color: white"
     homology        8745..8748
                     /label="GGAG"
     misc_feature    8749..9107
                     /label="From pich88103"
     Promoter        8749..9107
                     /label="ocs- octopine synthase promoter and 5' UTR (Agrobacterium tumefaciens)"
     misc_feature    8749..9107
                     /label="Pro-AtuOcs_5U-AtuOcs"
     homology        9108..9111
                     /label="AATG"
     misc_feature    9109..10539
                     /label="tdTomato"
     misc_feature    9112..10539
                     /label="From ec36254-pl0m-sc-tdtomato"
     primer          9611..9630
                     /label="tdTomato_F1"
                     /note="note: sequence: TGAAAGACGGAGGGCATTAC"
     primer          9611..9630
                     /label="tdTomato_F1"
                     /note="sequence: TGAAAGACGGAGGGCATTAC"
     primer          9829..9848
                     /label="tdTomato_F2"
                     /note="note: sequence: GCCTCATCCGAGGATAACAA"
     primer          9829..9848
                     /label="tdTomato_F2"
                     /note="sequence: GCCTCATCCGAGGATAACAA"
     primer          complement(10057..10076)
                     /label="tdTomato_R1"
                     /note="note: sequence: GGAATGTCAGCAGGGTGTTT"
     primer          complement(10057..10076)
                     /label="tdTomato_R1"
                     /note="sequence: GGAATGTCAGCAGGGTGTTT"
     primer          complement(10297..10316)
                     /label="tdTomato_R2"
                     /note="note: sequence: TCGCCCTTTAAGACACCATC"
     primer          complement(10297..10316)
                     /label="tdTomato_R2"
                     /note="sequence: TCGCCCTTTAAGACACCATC"
     homology        10540..10543
                     /label="GCTT"
     misc_feature    10544..11257
                     /label="From ec41432-pl0m-t-ocs"
     Terminator      10544..11257
                     /label="ocst"
     misc_feature    10544..11257
                     /label="3U+Ter-AtuOcs"
     primer          11038..11059
                     /label="TB0121"
                     /note="sequence: GTTGAGTATTGGCCGTCCGCTC"
     primer          11055..11073
                     /label="SEQ020"
                     /note="note: sequence: cgctctaccgaaagttacg"
     primer          11055..11073
                     /label="SEQ020"
                     /note="sequence: cgctctaccgaaagttacg"
     primer          11055..11073
                     /label="SEQ020"
                     /note="sequence: cgctctaccgaaagttacg"
     homology        11258..11261
                     /label="CGCT"
     homology        11263..11265
                     /label="CAG"
                     /note="color: #f7e8f7"
     misc_feature    11266..11475
                     /label="From ec36458-pck4-spacer"
                     /note="source: ec36458-pck4-spacer indicates_part: True color: white"
     misc_feature    11271..11470
                     /label="Odd-spacer"
                     /note="ApEinfo_revcolor: #d6b295"
ORIGIN
        1 ggtaaatgtg agaccggatc ctgacaggat atattggcgg gtaaacctaa gagaaaagag
       61 cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt
      121 tgtatgtgca tgccaaccac agggttcccc tcgggatcaa agtactttga tccaacccct
      181 ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcatctg aaaacgacat
      241 gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct
      301 tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc
      361 catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca
      421 ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga
      481 gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg
      541 ccctggcgac gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct
      601 actggacatt gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc
      661 gtgggccgac accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc
      721 cgagttcgag cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc
      781 ccgaggcgtg aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg
      841 cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca
      901 tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag
      961 gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga
     1021 gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt
     1081 ttcattaccg aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc
     1141 gcgcacctct caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg
     1201 gcggcctggc cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga
     1261 tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta
     1321 aataaacaaa tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg
     1381 ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg
     1441 atgttctgtt agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg
     1501 aagatcaacc gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg
     1561 ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg
     1621 tgtccgcgat caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca
     1681 tatgggccac cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa
     1741 ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg
     1801 ttgccgaggc gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg
     1861 tgagctaccc aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg
     1921 acgctgcccg cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta
     1981 atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc
     2041 acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt
     2101 caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg
     2161 caagaccatt accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa
     2221 atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac
     2281 aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg
     2341 taagcggctg ggttgcctgc cggccctgca atggcactgg aacccccaag cccgaggaat
     2401 cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga
     2461 cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc
     2521 acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc
     2581 gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt
     2641 tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc
     2701 cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc
     2761 agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga
     2821 cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa
     2881 gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg
     2941 gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac
     3001 cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc
     3061 cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga
     3121 gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc
     3181 ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct
     3241 ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat
     3301 ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct
     3361 gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc
     3421 gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg
     3481 tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggactctt
     3541 tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta
     3601 cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa
     3661 agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac
     3721 ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagtgctgc aaaaagcgcc
     3781 tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc
     3841 tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc
     3901 gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg
     3961 atgacggtga aaacctctga cacatgcagc tcccggtgac ggtcacagct tgtctgtaag
     4021 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     4081 gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc
     4141 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     4201 aaggagaaaa taccgcatca ggcgctcatc cgcttcctcg ctcactgact cgctgcgctc
     4261 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     4321 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     4381 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     4441 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     4501 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     4561 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     4621 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     4681 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     4741 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     4801 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     4861 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     4921 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     4981 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     5041 cgaaaactca cgttaaggga ttttggtcat gcattctagg tgattatttg ccgactacct
     5101 tggtgatctc gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca
     5161 agcgatcttc ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact
     5221 gggccggcag gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg
     5281 ttactgcgct gtaccaaatg cgggacaacg taagcactac atttcgctca tcaccagccc
     5341 agtcgggcgg cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt
     5401 caggaaccgg atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc
     5461 ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg
     5521 caagaatgtc attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc
     5581 acggaatgat gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct
     5641 ctccagggga agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat
     5701 caagccttac ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat
     5761 ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga
     5821 cgccaactac ctctgatagt tgagtcgata cttcggcgat caccgcttcc ctcataatgt
     5881 ttaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca
     5941 aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc
     6001 caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt
     6061 cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta cttgcattac agcttacgaa
     6121 ccgaacaggc ttatgtccac tgggttcgtg ccttcatccg tttccacggt gtgcgtcacc
     6181 cggcaacctt gggtagcagc gaagtcgagg catttctgtc ctggctggaa cagaacttat
     6241 tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat tataacaaga
     6301 cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa acagcttttt
     6361 caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt ttgaaaccgc
     6421 ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca acatgctacc ctccgcgaga
     6481 tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt ccgaatagca tcggtaacat
     6541 gagcaaagtc tgccgcctta caacggctct cccgctgacg ccgtcccgga ctgatgggct
     6601 gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg
     6661 tggcaggata tattgtggtg taaacataac ggatccggtc tcatactaat gaggagtctg
     6721 agcaggacaa ctcgcgtagt gagagttaca tgttcgttgg gttcttccga cacggacctg
     6781 agttggccaa cgtcccacct gaggtctgtg ccccggtgat gagaagtgtg catctcgttc
     6841 ttgcagctcg tcagtacttt cagaatcatg gcgtgcatgg tagaatgacc cttataacgg
     6901 acttcgacat ggcaatcgct agcaaGGAGG TCAACATGGT GGAGCACGAC ACTCTGGTCT
     6961 ACTCCAAAAA TGTCAAAGAT ACAGTCTCAG AAGATCAAAG GGCTATTGAG ACTTTTCAAC
     7021 AAAGGATAAT TTCGGGAAAC CTCCTCGGAT TCCATTGCCC AGCTATCTGT CACTTCATCG
     7081 AAAGGACAGT AGAAAAGGAA GGTGGCTCCT ACAAATGCCA TCATTGCGAT AAAGGAAAGG
     7141 CTATCATTCA AGATCTCTCT GCCGACAGTG GTCCCAAAGA TGGACCCCCA CCCACGAGGA
     7201 GCATCGTGGA AAAAGAAGAG GTTCCAACCA CGTCTACAAA GCAAGTGGAT TGATGTGACA
     7261 TCTCCACTGA CGTAAGGGAT GACGCACAAT CCCACTATCC TTCGCAAGAC CCTTCCTCTA
     7321 TATAAGGAAG TTCATTTCAT TTGGAGAGGA CACGCtacta agtcttccag gaagggatgg
     7381 ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac agggactggt
     7441 gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat
     7501 gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca
     7561 cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa
     7621 agctggcgca tgatgaccac cgatatggcc agtgtgccgg tatccgttat cggggaagaa
     7681 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
     7741 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacg atatctctag
     7801 agctcgaaga cttaatgGTG AGCAAAGGGG AGGAGGACAA TATGGCATCA CTGCCTGCAA
     7861 CACATGAGCT GCATATATTC GGTTCTATCA ATGGAGTTGA TTTTGACATG GTCGGGCAGG
     7921 GCACTGGCAA CCCCAACGAT GGATACGAGG AACTGAACTT AAAGAGCACA AAGGGGGATC
     7981 TTCAATTCAG CCCCTGGATA CTCGTACCGC ACATAGGTTA TGGCTTTCAC CAATATCTTC
     8041 CCTATCCAGA CGGCATGTCC CCCTTTCAAG CTGCAATGGT CGACGGAAGT GGGTACCAAG
     8101 TTCATCGTAC GATGCAATTC GAGGACGGGG CTTCTCTGAC GGTGAACTAC AGATATACAT
     8161 ACGAAGGCTC ACACATAAAA GGAGAAGCTC AGGTAAAGGG TACGGGTTTT CCCGCAGATG
     8221 GCCCTGTAAT GACTAACTCC CTGACTGCCG CAGATTGGTG CCGATCTAAA AAGACTTATC
     8281 CAAATGATAA AACTATCATA AGCACGTTTA AGTGGTCCTA TACGACAGGA AATGGTAAAA
     8341 GGTACAGATC AACGGCAAGG ACCACATACA CATTTGCAAA GCCGATGGCA GCTAACTACC
     8401 TTAAAAATCA GCCCATGTAC GTGTTCAGGA AAACAGAATT AAAACACTCT AAGACGGAGC
     8461 TTAATTTCAA GGAGTGGCAG AAGGCTTTCA CGGATGTGAT GGGCATGGAC GAACTCTATA
     8521 AATGAgcttg agtccgcaaa aatcaccagt ctctctctac aaatctatct ctctctattt
     8581 ttctccagaa taatgtgtga gtagttccca gataagggaa ttagggttct tatagggttt
     8641 cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt aaaatacttc
     8701 tatcaataaa atttctaatt cctaaaacca aaatccgcta tacaggagCT GAAAGCGACG
     8761 TTGGATGTTA ACATCTACAA ATTGCCTTTT CTTATCGACC ATGTACGTAA GCGCTTACGT
     8821 TTTTGGTGGA CCCTTGAGGA AACTGGTAGC TGTTGTGGGC CTGTGCTCTC AAGATGGATC
     8881 ATTAATTTCC ACCTTCACCT ACGATGGGGG GCATCGCACC GGTGAGTAAT ATTGTACGGC
     8941 TAAGAGCGAA TTTGGCCTGT AAGATCCTTT TTACCGACAA CTCATCCACA TTGATGGTAG
     9001 GCAGAAAGTT AAAGGATTAT CGCAAGTCAA TACTTGCCCA TTCATTGATC TATTTAAAGG
     9061 TGTGGCCTCA AGGATAATCG CCAAACCATT ATATTTGCAA TCTACCAaat gGTATCCAAG
     9121 GGGGAAGAAG TAATAAAAGA GTTCATGCGA TTCAAGGTTC GTATGGAGGG CTCAATGAAC
     9181 GGCCACGAAT TTGAGATAGA AGGGGAGGGT GAGGGACGTC CATATGAAGG CACGCAGACG
     9241 GCAAAATTAA AGGTCACGAA AGGAGGCCCG TTACCCTTCG CCTGGGATAT TTTGTCTCCT
     9301 CAGTTCATGT ATGGGAGTAA AGCCTATGTT AAGCACCCCG CTGATATACC GGACTACAAA
     9361 AAACTGTCTT TTCCCGAAGG GTTCAAATGG GAAAGGGTCA TGAATTTCGA GGATGGCGGT
     9421 TTGGTTACTG TCACCCAAGA TAGTTCACTG CAAGATGGAA CATTAATATA CAAAGTGAAG
     9481 ATGCGAGGAA CCAACTTCCC ACCGGACGGC CCGGTCATGC AGAAGAAAAC GATGGGATGG
     9541 GAAGCTAGTA CCGAACGTCT GTATCCTAGG GACGGAGTTC TTAAGGGCGA GATCCACCAG
     9601 GCTTTGAAAC TGAAAGACGG AGGGCATTAC CTGGTGGAAT TTAAGACAAT CTACATGGCT
     9661 AAAAAGCCCG TGCAGCTCCC CGGGTATTAC TACGTGGACA CCAAGCTGGA CATCACGAGT
     9721 CATAATGAAG ATTACACCAT TGTAGAGCAA TATGAAAGAA GTGAAGGGCG TCATCATCTT
     9781 TTTTTAGGCC ATGGTACAGG CAGTACTGGA TCCGGCTCCT CCGGGACCGC CTCATCCGAG
     9841 GATAACAACA TGGCAGTCAT TAAGGAATTT ATGAGGTTTA AAGTTCGAAT GGAAGGATCA
     9901 ATGAACGGGC ACGAATTTGA AATCGAGGGA GAGGGAGAAG GTCGACCCTA CGAAGGCACA
     9961 CAAACCGCTA AACTTAAGGT GACTAAAGGT GGTCCGCTGC CATTCGCATG GGATATACTT
    10021 TCACCCCAAT TCATGTACGG GTCAAAAGCA TATGTGAAAC ACCCTGCTGA CATTCCAGAT
    10081 TATAAGAAAC TCAGTTTCCC AGAAGGATTT AAATGGGAGA GGGTTATGAA TTTTGAGGAT
    10141 GGAGGTTTGG TTACGGTAAC ACAGGATTCC AGCCTCCAGG ACGGTACTCT GATCTACAAG
    10201 GTCAAAATGC GTGGCACTAA CTTTCCGCCG GACGGCCCAG TGATGCAAAA GAAAACGATG
    10261 GGCTGGGAGG CATCAACTGA ACGATTGTAT CCCCGTGATG GTGTCTTAAA GGGCGAGATA
    10321 CATCAAGCTT TGAAATTGAA GGACGGGGGG CACTATCTGG TAGAGTTTAA AACAATCTAT
    10381 ATGGCCAAAA AACCAGTGCA ATTGCCCGGA TACTACTATG TAGACACAAA GTTAGACATT
    10441 ACGAGTCATA ATGAAGATTA CACCATCGTC GAACAGTATG AACGATCAGA AGGTAGACAT
    10501 CATCTTTTCT TATATGGAAT GGACGAATTG TACAAGTGAg cttGTCCTGC TTTAATGAGA
    10561 TATGCGAGAA GCCTATGATC GCATGATATT TGCTTTCAAT TCTGTTGTGC ACGTTGTAAA
    10621 AAACCTGAGC ATGTGTAGCT CAGATCCTTA CCGCCGGTTT CGGTTCATTC TAATGAATAT
    10681 ATCACCCGTT ACTATCGTAT TTTTATGAAT AATATTCTCC GTTCAATTTA CTGATTGTAC
    10741 CCTACTACTT ATATGTACAA TATTAAAATG AAAACAATAT ATTGTGCTGA ATAGGTTTAT
    10801 AGCGACATCT ATGATAGAGC GCCACAATAA CAAACAATTG CGTTTTATTA TTACAAATCC
    10861 AATTTTAAAA AAAGCGGCAG AACCGGTCAA ACCTAAAAGA CTGATTACAT AAATCTTATT
    10921 CAAATTTCAA AAGTGCCCCA GGGGCTAGTA TCTACGACAC ACCGAGCGGC GAACTAATAA
    10981 CGCTCACTGA AGGGAACTCC GGTTCCCCGC CGGCGCGCAT GGGTGAGATT CCTTGAAGTT
    11041 GAGTATTGGC CGTCCGCTCT ACCGAAAGTT ACGGGCACCA TTCAACCCGG TCCAGCACGG
    11101 CGGCCGGGTA ACCGACTTGC TGCCCCGAGA ATTATGCAGC ATTTTTTTGG TGTATGTGGG
    11161 CCCCAAATGA AGTGCAGGTC AAACCTTGAC AGTGACGACA AATCGTTGGG CGGGTCCAGG
    11221 GCGAATTTTG CGACAACATG TCGAGGCTCA GCAGGACcgc tacagaggag tctgagcagg
    11281 acaactcgcg tagtgagagt tacatgttcg ttgggttctt ccgacacgga cctgagttgg
    11341 ccaacgtccc acctgaggtc tgtgccccgg tgatgagaag tgtgcatctc gttcttgcag
    11401 ctcgtcagta ctttcagaat catggcgtgc atggtagaat gacccttata acggacttcg
    11461 acatggcaat cgcta
//