LOCUS       Exported                8467 bp ds-DNA     circular SYN 29-AUG-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8467)
  AUTHORS   NIH-NCI
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Sep 13, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8467
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV Forward

     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
     regulatory      1009..1018
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1021..1791
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     primer_bind     1779..1795
                     /label=A89-MiniTurbo-sequencing-FP
     CDS             1834..1899
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     1922..1967
                     /label=A3-TAF5-GC-attB1-FP
     protein_bind    1924..1948
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1951..4348
                     /codon_start=1
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /product="transcription initiation factor TFIID subunit 5
                     isoform 1"
                     /label=TAF5
                     /note="isoform 1 is encoded by transcript variant 1; TATA
                     box binding protein (TBP)-associated factor, RNA polymerase
                     II, D, 100kD; TATA box binding protein (TBP)-associated 
                     factor 2D; transcription initiation factor TFIID 100 kD 
                     subunit; transcription initiation factor TFIID subunit 5; 
                     transcription initiation factor TFIID 100 kDa subunit; TAF5
                     RNA polymerase II, TATA box binding protein 
                     (TBP)-associated factor, 100kDa"
                     /db_xref="CCDS:CCDS7547.1"
                     /db_xref="GeneID:6877"
                     /db_xref="HGNC:HGNC:11539"
                     /db_xref="MIM:601787"
                     /protein_id="NP_008882.2"
                     /translation="AALAEEQTEVAVKLEPEGPPTLLPPQAGDGAGEGSGGTTNNGPNG
                     GGGNVAASSSTGGDGGTPKPTVAVSAAAPAGAAPVPAAAPDAGAPHDRQTLLAVLQFLR
                     QSKLREAEEALRREAGLLEEAVAGSGAPGEVDSAGAEVTSALLSRVTASAPGPAAPDPP
                     GTGASGATVVSGSASGPAAPGKVGSVAVEDQPDVSAVLSAYNQQGDPTMYEEYYSGLKH
                     FIECSLDCHRAELSQLFYPLFVHMYLELVYNQHENEAKSFFEKFHGDQECYYQDDLRVL
                     SSLTKKEHMKGNETMLDFRTSKFVLRISRDSYQLLKRHLQEKQNNQIWNIVQEHLYIDI
                     FDGMPRSKQQIDAMVGSLAGEAKREANKSKVFFGLLKEPEIEVPLDDEDEEGENEEGKP
                     KKKKPKKDSIGSKSKKQDPNAPPQNRIPLPELKDSDKLDKIMNMKETTKRVRLGPDCLP
                     SICFYTFLNAYQGLTAVDVTDDSSLIAGGFADSTVRVWSVTPKKLRSVKQASDLSLIDK
                     ESDDVLERIMDEKTASELKILYGHSGPVYGASFSPDRNYLLSSSEDGTVRLWSLQTFTC
                     LVGYKGHNYPVWDTQFSPYGYYFVSGGHDRVARLWATDHYQPLRIFAGHLADVNCTRFH
                     PNSNYVATGSADRTVRLWDVLNGNCVRIFTGHKGPIHSLTFSPNGRFLATGATDGRVLL
                     WDIGHGLMVGELKGHTDTVCSLRFSRDGEILASGSMDNTVRLWDAIKAFEDLETDDFTT
                     ATGHINLPENSQELLLGTYMTKSTPVVHLHFTRRNLVLAAGAYSPQ"
     exon            1951..2506
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /inference="alignment:same species:2.1.0"
     exon            2507..2744
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /inference="alignment:same species:2.1.0"
     exon            2745..3060
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /inference="alignment:same species:2.1.0"
     exon            3061..3224
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /inference="alignment:same species:2.1.0"
     exon            3225..3360
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /inference="alignment:same species:2.1.0"
     misc_feature    3349..3468
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /note="propagated from UniProtKB/Swiss-Prot (Q15542.3);
                     Region: WD 1"
     exon            3361..3481
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /inference="alignment:same species:2.1.0"
     primer_bind     complement(3436..3453)
                     /label=A79-TAF5-SDM1 to WT-AtoG-RP
     primer_bind     3454..3476
                     /label=A78-TAF5-SDM1 to WT-AtoG-FP
     exon            3482..3611
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /label=exon 7
                     /inference="alignment:same species:2.1.0"
     misc_feature    3568..3687
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /note="propagated from UniProtKB/Swiss-Prot (Q15542.3);
                     Region: WD 2"
     primer_bind     complement(3594..3611)
                     /label=A44-TAF5-dexon8-RP
     exon            3612..3776
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /label=exon 8
                     /inference="alignment:same species:2.1.0"
     misc_feature    3694..3819
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /note="propagated from UniProtKB/Swiss-Prot (Q15542.3);
                     Region: WD 3"
     exon            3777..3954
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /label=exon 9
                     /inference="alignment:same species:2.1.0"
     primer_bind     3777..3794
                     /label=A43-TAF5-dexon8-FP
     misc_feature    3820..3945
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /note="propagated from UniProtKB/Swiss-Prot (Q15542.3);
                     Region: WD 4"
     misc_feature    3946..4065
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /note="propagated from UniProtKB/Swiss-Prot (Q15542.3);
                     Region: WD 5"
     exon            3955..4132
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /inference="alignment:same species:2.1.0"
     misc_feature    4072..4191
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /note="propagated from UniProtKB/Swiss-Prot (Q15542.3);
                     Region: WD 6"
     exon            4133..4348
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /inference="alignment:same species:2.1.0"
     primer_bind     complement(4277..4296)
                     /label=A81-TAF5-SDM2 to WT-TtoG-FP
     primer_bind     4297..4321
                     /label=A80-TAF5-SDM2 to WT-TtoG-FP
     primer_bind     complement(4321..4377)
                     /label=A4-TAF5-GC-attB2-RP
     regulatory      4346..4348
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /label=hexamer: AATAAA
                     /note="hexamer: AATAAA"
     protein_bind    complement(4353..4377)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4419..4436)
                     /label=BGH Reverse
     polyA_signal    4425..4649
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    4933..4980
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4988..6010
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6140..6261
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6310..6326)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6334..6350
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6358..6388)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6403..6424
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(6712..7300)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7471..8331)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8332..8436)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccg agctcggatc cagctagcgc caccatggcg
     1021 atcccgctgc tgaacgctaa acagattctg ggacagctgg acggcgggag cgtggcagtc
     1081 ctgcctgtgg tcgactccac caatcagtac ctgctggatc gaatcggcga gctgaagagt
     1141 ggggatgctt gcattgcaga atatcagcag gcagggagag gaagcagagg gaggaaatgg
     1201 ttctctcctt ttggagctaa cctgtacctg agtatgtttt ggcgcctgaa gcggggacca
     1261 gcagcaatcg gcctgggccc ggtcatcgga attgtcatgg cagaagcgct gcgaaagctg
     1321 ggagcagaca aggtgcgagt caaatggccc aatgacctgt atctgcagga tagaaagctg
     1381 gcaggcatcc tggtggagct ggccggaata acaggcgatg ctgcacagat cgtcattggc
     1441 gccgggatta acgtggctat gaggcgcgtg gaggaaagcg tggtcaatca gggctggatc
     1501 acactgcagg aagcagggat taacctggac aggaatactc tggccgctat gctgatccga
     1561 gagctgcggg cagccctgga actgttcgag caggaaggcc tggctccata tctgtcacgg
     1621 tgggagaagc tggataactt catcaataga cccgtgaagc tgatcattgg ggacaaagag
     1681 attttcggga ttagccgggg gattgataaa cagggagccc tgctgctgga acaggacgga
     1741 gttatcaaac cctggatggg cggagaaatc agtctgcggt ctgccgaaaa gatgcatggg
     1801 ggagggggag gatctggggg agggggagga tctgactaca aggaccacga cggtgactac
     1861 aaggaccacg acatcgacta caaggacgac gacgacaagt taattaaggg cgcgccacct
     1921 aggacaagtt tgtacaaaaa agcaggcttc gcggcgctgg cggaggagca gacggaggtg
     1981 gcggtcaagc tagagcctga gggaccgcca acgctgctac ctccgcaggc gggggacggc
     2041 gcaggcgagg gtagcggcgg cactaccaac aacggcccca acggcggcgg cgggaacgtt
     2101 gcggcgtcgt cgtccactgg cggggatggc gggaccccca agcccacggt ggctgtctcc
     2161 gccgctgccc cggcgggggc ggccccggtg cccgccgctg ctccggacgc cggcgctccg
     2221 catgaccgac agactctact ggccgtgctg cagttcctac ggcagagcaa actccgcgag
     2281 gccgaagagg cgctgcgccg tgaggccggg ctgctggagg aggcagtggc gggctccgga
     2341 gccccgggag aggtggacag cgccggcgct gaggtgacca gcgcgcttct cagccgggtg
     2401 accgcctcgg cccctggccc tgcggccccc gaccctccgg gcactggcgc ttcgggggcc
     2461 acggtcgtct caggttcagc ctcaggtcct gcggctccgg gtaaagttgg aagtgttgct
     2521 gtggaagacc agccagatgt cagtgccgtg ttgtcagcct acaaccaaca aggagatccc
     2581 acaatgtatg aagaatacta tagtggactg aaacacttca ttgaatgttc cctggactgc
     2641 catcgggcag agttgtccca acttttttat cctctgtttg tgcacatgta cttggagcta
     2701 gtctacaatc aacatgagaa tgaagcaaag tcattctttg agaagttcca tggagatcag
     2761 gaatgttatt accaggatga cctacgagta ttatctagtc ttaccaaaaa ggaacacatg
     2821 aaagggaatg agaccatgtt ggattttcga acaagtaaat ttgttctgcg tatttcccgt
     2881 gactcgtacc aactcttgaa gaggcatctt caggagaaac agaacaatca gatatggaac
     2941 atagttcagg agcacctcta cattgacatc tttgatggga tgccgcgtag taagcaacag
     3001 atagatgcga tggtgggaag tttggcagga gaggctaaac gagaggcaaa caaatcaaag
     3061 gtattttttg gtttattaaa agaaccagaa attgaggtac ctttggatga cgaggatgaa
     3121 gagggagaaa atgaagaagg aaaacctaaa aagaagaagc ctaaaaaaga tagtattgga
     3181 tccaaaagca aaaaacaaga tcccaatgct ccacctcaga acagaatccc tcttcctgag
     3241 ttgaaagatt cagataagtt ggataagata atgaatatga aagaaaccac caaacgagtg
     3301 cgccttgggc cggactgctt accctccatt tgtttctata catttctcaa tgcttaccag
     3361 ggtctcactg cagtggatgt cactgatgat tctagtctga ttgctggagg ttttgcagat
     3421 tcaactgtca gagtgtggtc ggtaacaccc aaaaagcttc gtagtgtcaa acaagcatca
     3481 gatcttagtc ttatagacaa agaatcagat gatgtcttag aaagaatcat ggatgagaaa
     3541 acagcaagtg agttgaagat tttgtatggt cacagtgggc ctgtctacgg agccagcttc
     3601 agtccggata ggaactatct gctttcctct tcagaggacg gaactgttag attgtggagc
     3661 cttcaaacat ttacttgttt ggtgggatat aaaggacaca actatccagt atgggacaca
     3721 caattttctc catatggata ttattttgtg tcagggggcc atgaccgagt agctcggctc
     3781 tgggctacag accactatca gcctttaaga atatttgccg gccatcttgc tgatgtgaat
     3841 tgtaccagat tccatccaaa ttctaattat gttgctacgg gctctgcaga cagaactgtg
     3901 cggctctggg acgtcctgaa tggtaactgt gtaaggatct tcactggaca caagggacca
     3961 attcattcct tgacattttc tcccaatggg agattcctgg ctacaggagc aacagatggc
     4021 agagtgcttc tttgggatat tggacatggt ttgatggttg gagaattaaa aggccacact
     4081 gatacagtct gttcacttag gtttagtaga gatggtgaaa ttttggcatc aggttcaatg
     4141 gataatacag ttcgattatg ggatgctatc aaagcctttg aagatttaga gaccgatgac
     4201 tttactacag ccactgggca tataaattta cctgagaatt cacaggagtt attgttggga
     4261 acatatatga ccaaatcaac accagttgta caccttcatt ttactcgaag aaacctggtt
     4321 ctagctgcag gagcttatag tccacaatga taacccagct ttcttgtaca aagtggtgtg
     4381 actcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     4441 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     4501 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     4561 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     4621 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     4681 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     4741 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     4801 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     4861 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     4921 ttcacgtacc tagaagttcc tattccgaag ttcctattct ctagaaagta taggaacttc
     4981 cttggccaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
     5041 cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
     5101 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
     5161 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
     5221 cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac
     5281 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat
     5341 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
     5401 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
     5461 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
     5521 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
     5581 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
     5641 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
     5701 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
     5761 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
     5821 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
     5881 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
     5941 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
     6001 aaaggaatag cacgtactac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     6061 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     6121 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     6181 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     6241 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     6301 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     6361 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     6421 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     6481 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     6541 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     6601 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     6661 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     6721 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     6781 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     6841 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     6901 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     6961 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     7021 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     7081 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     7141 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     7201 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     7261 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     7321 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     7381 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     7441 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     7501 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     7561 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     7621 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     7681 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     7741 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     7801 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     7861 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     7921 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     7981 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     8041 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     8101 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     8161 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     8221 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     8281 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     8341 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     8401 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     8461 tgacgtc
//
