LOCUS       Exported                8302 bp ds-DNA     circular SYN 29-AUG-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8302)
  AUTHORS   Damodaran, Arun Prasath (NIH/NCI) [F]
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Sep 13, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8302
                     /organism="synthetic DNA construct"
                     /lab_host="Homo sapiens"
                     /mol_type="other DNA"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV Forward

     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
     regulatory      1009..1018
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1021..1791
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     primer_bind     1779..1795
                     /label=A89-MiniTurbo-sequencing-FP
     CDS             1834..1899
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     1922..1967
                     /label=A3-TAF5-GC-attB1-FP
     protein_bind    1924..1948
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1951..4183
                     /codon_start=1
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /product="transcription initiation factor TFIID subunit 5
                     isoform 1"
                     /label=TAF5
                     /note="isoform 1 is encoded by transcript variant 1; TATA
                     box binding protein (TBP)-associated factor, RNA polymerase
                     II, D, 100kD; TATA box binding protein (TBP)-associated 
                     factor 2D; transcription initiation factor TFIID 100 kD 
                     subunit; transcription initiation factor TFIID subunit 5; 
                     transcription initiation factor TFIID 100 kDa subunit; TAF5
                     RNA polymerase II, TATA box binding protein 
                     (TBP)-associated factor, 100kDa"
                     /db_xref="CCDS:CCDS7547.1"
                     /db_xref="GeneID:6877"
                     /db_xref="HGNC:HGNC:11539"
                     /db_xref="MIM:601787"
                     /protein_id="NP_008882.2"
                     /translation="AALAEEQTEVAVKLEPEGPPTLLPPQAGDGAGEGSGGTTNNGPNG
                     GGGNVAASSSTGGDGGTPKPTVAVSAAAPAGAAPVPAAAPDAGAPHDRQTLLAVLQFLR
                     QSKLREAEEALRREAGLLEEAVAGSGAPGEVDSAGAEVTSALLSRVTASAPGPAAPDPP
                     GTGASGATVVSGSASGPAAPGKVGSVAVEDQPDVSAVLSAYNQQGDPTMYEEYYSGLKH
                     FIECSLDCHRAELSQLFYPLFVHMYLELVYNQHENEAKSFFEKFHGDQECYYQDDLRVL
                     SSLTKKEHMKGNETMLDFRTSKFVLRISRDSYQLLKRHLQEKQNNQIWNIVQEHLYIDI
                     FDGMPRSKQQIDAMVGSLAGEAKREANKSKVFFGLLKEPEIEVPLDDEDEEGENEEGKP
                     KKKKPKKDSIGSKSKKQDPNAPPQNRIPLPELKDSDKLDKIMNMKETTKRVRLGPDCLP
                     SICFYTFLNAYQGLTAVDVTDDSSLIAGGFADSTVRVWSVTPKKLRSVKQASDLSLIDK
                     ESDDVLERIMDEKTASELKILYGHSGPVYGASFSPDRLWATDHYQPLRIFAGHLADVNC
                     TRFHPNSNYVATGSADRTVRLWDVLNGNCVRIFTGHKGPIHSLTFSPNGRFLATGATDG
                     RVLLWDIGHGLMVGELKGHTDTVCSLRFSRDGEILASGSMDNTVRLWDAIKAFEDLETD
                     DFTTATGHINLPENSQELLLGTYMTKSTPVVHLHFTRRNLVLAAGAYSPQ"
     exon            1951..2506
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /inference="alignment:same species:2.1.0"
     exon            2507..2744
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /inference="alignment:same species:2.1.0"
     exon            2745..3060
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /inference="alignment:same species:2.1.0"
     exon            3061..3224
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /inference="alignment:same species:2.1.0"
     exon            3225..3360
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /inference="alignment:same species:2.1.0"
     misc_feature    3349..3468
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /note="propagated from UniProtKB/Swiss-Prot (Q15542.3);
                     Region: WD 1"
     exon            3361..3481
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /inference="alignment:same species:2.1.0"
     primer_bind     complement(3436..3453)
                     /label=A79-TAF5-SDM1 to WT-AtoG-RP
     primer_bind     3454..3476
                     /label=A78-TAF5-SDM1 to WT-AtoG-FP
     exon            3482..3611
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /label=exon 7
                     /inference="alignment:same species:2.1.0"
     misc_feature    3568..3611
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /note="propagated from UniProtKB/Swiss-Prot (Q15542.3);
                     Region: WD 2"
     primer_bind     complement(3594..3611)
                     /label=A44-TAF5-dexon8-RP
     exon            3612..3789
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /label=exon 9
                     /inference="alignment:same species:2.1.0"
     misc_feature    3612..3654
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /note="propagated from UniProtKB/Swiss-Prot (Q15542.3);
                     Region: WD 3"
     primer_bind     3612..3629
                     /label=A43-TAF5-dexon8-FP
     misc_feature    3655..3780
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /note="propagated from UniProtKB/Swiss-Prot (Q15542.3);
                     Region: WD 4"
     misc_feature    3781..3900
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /note="propagated from UniProtKB/Swiss-Prot (Q15542.3);
                     Region: WD 5"
     exon            3790..3967
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /inference="alignment:same species:2.1.0"
     misc_feature    3907..4026
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /note="propagated from UniProtKB/Swiss-Prot (Q15542.3);
                     Region: WD 6"
     exon            3968..4183
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /inference="alignment:same species:2.1.0"
     primer_bind     complement(4112..4131)
                     /label=A81-TAF5-SDM2 to WT-TtoG-FP
     primer_bind     4132..4156
                     /label=A80-TAF5-SDM2 to WT-TtoG-FP
     primer_bind     complement(4156..4212)
                     /label=A4-TAF5-GC-attB2-RP
     regulatory      4181..4183
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TAF5"
                     /gene_synonym="TAF(II)100; TAF2D; TAFII-100; TAFII100"
                     /label=hexamer: AATAAA
                     /note="hexamer: AATAAA"
     protein_bind    complement(4188..4212)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4254..4271)
                     /label=BGH Reverse
     polyA_signal    4260..4484
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    4768..4815
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4823..5845
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5975..6096
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6145..6161)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6169..6185
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6193..6223)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6238..6259
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(6547..7135)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7306..8166)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8167..8271)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccg agctcggatc cagctagcgc caccatggcg
     1021 atcccgctgc tgaacgctaa acagattctg ggacagctgg acggcgggag cgtggcagtc
     1081 ctgcctgtgg tcgactccac caatcagtac ctgctggatc gaatcggcga gctgaagagt
     1141 ggggatgctt gcattgcaga atatcagcag gcagggagag gaagcagagg gaggaaatgg
     1201 ttctctcctt ttggagctaa cctgtacctg agtatgtttt ggcgcctgaa gcggggacca
     1261 gcagcaatcg gcctgggccc ggtcatcgga attgtcatgg cagaagcgct gcgaaagctg
     1321 ggagcagaca aggtgcgagt caaatggccc aatgacctgt atctgcagga tagaaagctg
     1381 gcaggcatcc tggtggagct ggccggaata acaggcgatg ctgcacagat cgtcattggc
     1441 gccgggatta acgtggctat gaggcgcgtg gaggaaagcg tggtcaatca gggctggatc
     1501 acactgcagg aagcagggat taacctggac aggaatactc tggccgctat gctgatccga
     1561 gagctgcggg cagccctgga actgttcgag caggaaggcc tggctccata tctgtcacgg
     1621 tgggagaagc tggataactt catcaataga cccgtgaagc tgatcattgg ggacaaagag
     1681 attttcggga ttagccgggg gattgataaa cagggagccc tgctgctgga acaggacgga
     1741 gttatcaaac cctggatggg cggagaaatc agtctgcggt ctgccgaaaa gatgcatggg
     1801 ggagggggag gatctggggg agggggagga tctgactaca aggaccacga cggtgactac
     1861 aaggaccacg acatcgacta caaggacgac gacgacaagt taattaaggg cgcgccacct
     1921 aggacaagtt tgtacaaaaa agcaggcttc gcggcgctgg cggaggagca gacggaggtg
     1981 gcggtcaagc tagagcctga gggaccgcca acgctgctac ctccgcaggc gggggacggc
     2041 gcaggcgagg gtagcggcgg cactaccaac aacggcccca acggcggcgg cgggaacgtt
     2101 gcggcgtcgt cgtccactgg cggggatggc gggaccccca agcccacggt ggctgtctcc
     2161 gccgctgccc cggcgggggc ggccccggtg cccgccgctg ctccggacgc cggcgctccg
     2221 catgaccgac agactctact ggccgtgctg cagttcctac ggcagagcaa actccgcgag
     2281 gccgaagagg cgctgcgccg tgaggccggg ctgctggagg aggcagtggc gggctccgga
     2341 gccccgggag aggtggacag cgccggcgct gaggtgacca gcgcgcttct cagccgggtg
     2401 accgcctcgg cccctggccc tgcggccccc gaccctccgg gcactggcgc ttcgggggcc
     2461 acggtcgtct caggttcagc ctcaggtcct gcggctccgg gtaaagttgg aagtgttgct
     2521 gtggaagacc agccagatgt cagtgccgtg ttgtcagcct acaaccaaca aggagatccc
     2581 acaatgtatg aagaatacta tagtggactg aaacacttca ttgaatgttc cctggactgc
     2641 catcgggcag agttgtccca acttttttat cctctgtttg tgcacatgta cttggagcta
     2701 gtctacaatc aacatgagaa tgaagcaaag tcattctttg agaagttcca tggagatcag
     2761 gaatgttatt accaggatga cctacgagta ttatctagtc ttaccaaaaa ggaacacatg
     2821 aaagggaatg agaccatgtt ggattttcga acaagtaaat ttgttctgcg tatttcccgt
     2881 gactcgtacc aactcttgaa gaggcatctt caggagaaac agaacaatca gatatggaac
     2941 atagttcagg agcacctcta cattgacatc tttgatggga tgccgcgtag taagcaacag
     3001 atagatgcga tggtgggaag tttggcagga gaggctaaac gagaggcaaa caaatcaaag
     3061 gtattttttg gtttattaaa agaaccagaa attgaggtac ctttggatga cgaggatgaa
     3121 gagggagaaa atgaagaagg aaaacctaaa aagaagaagc ctaaaaaaga tagtattgga
     3181 tccaaaagca aaaaacaaga tcccaatgct ccacctcaga acagaatccc tcttcctgag
     3241 ttgaaagatt cagataagtt ggataagata atgaatatga aagaaaccac caaacgagtg
     3301 cgccttgggc cggactgctt accctccatt tgtttctata catttctcaa tgcttaccag
     3361 ggtctcactg cagtggatgt cactgatgat tctagtctga ttgctggagg ttttgcagat
     3421 tcaactgtca gagtgtggtc ggtaacaccc aaaaagcttc gtagtgtcaa acaagcatca
     3481 gatcttagtc ttatagacaa agaatcagat gatgtcttag aaagaatcat ggatgagaaa
     3541 acagcaagtg agttgaagat tttgtatggt cacagtgggc ctgtctacgg agccagcttc
     3601 agtccggata ggctctgggc tacagaccac tatcagcctt taagaatatt tgccggccat
     3661 cttgctgatg tgaattgtac cagattccat ccaaattcta attatgttgc tacgggctct
     3721 gcagacagaa ctgtgcggct ctgggacgtc ctgaatggta actgtgtaag gatcttcact
     3781 ggacacaagg gaccaattca ttccttgaca ttttctccca atgggagatt cctggctaca
     3841 ggagcaacag atggcagagt gcttctttgg gatattggac atggtttgat ggttggagaa
     3901 ttaaaaggcc acactgatac agtctgttca cttaggttta gtagagatgg tgaaattttg
     3961 gcatcaggtt caatggataa tacagttcga ttatgggatg ctatcaaagc ctttgaagat
     4021 ttagagaccg atgactttac tacagccact gggcatataa atttacctga gaattcacag
     4081 gagttattgt tgggaacata tatgaccaaa tcaacaccag ttgtacacct tcattttact
     4141 cgaagaaacc tggttctagc tgcaggagct tatagtccac aatgataacc cagctttctt
     4201 gtacaaagtg gtgtgactcg agtctagagg gcccgtttaa acccgctgat cagcctcgac
     4261 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     4321 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     4381 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     4441 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     4501 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     4561 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     4621 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     4681 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     4741 tgattagggt gatggttcac gtacctagaa gttcctattc cgaagttcct attctctaga
     4801 aagtatagga acttccttgg ccaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt
     4861 tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc
     4921 tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc
     4981 cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat
     5041 tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg
     5101 tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc
     5161 ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg
     5221 cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat
     5281 tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt
     5341 cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct
     5401 cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt
     5461 cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt
     5521 ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc
     5581 ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact
     5641 ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga
     5701 cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc
     5761 ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag
     5821 cactcgtccg agggcaaagg aatagcacgt actacgagat ttcgattcca ccgccgcctt
     5881 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     5941 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     6001 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     6061 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     6121 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     6181 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     6241 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     6301 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     6361 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     6421 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     6481 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     6541 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     6601 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     6661 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     6721 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     6781 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     6841 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     6901 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     6961 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     7021 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     7081 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     7141 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     7201 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     7261 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     7321 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     7381 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     7441 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     7501 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     7561 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     7621 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     7681 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     7741 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     7801 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     7861 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     7921 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     7981 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     8041 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     8101 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     8161 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     8221 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     8281 ccgaaaagtg ccacctgacg tc
//
