LOCUS       pLeo-A-GEMS(JAK-STAT)   6723 bp ds-DNA     circular     12-SEP-2023
DEFINITION  .
KEYWORDS    "creator:Trial User" "marker:NeoR/KanR, AmpR"
COMMENT     Imported using the Genbank importer. File name: pleo-a-gems.gb
FEATURES             Location/Qualifiers
     polyA_signal    963..1084
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: SV40 polyadenylation signal"
     primer_bind     complement(1133..1149)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
     protein_bind    1157..1173
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: lac repressor encoded by lacI note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1181..1187)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(1188..1205)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(1206..1211)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     protein_bind    1226..1247
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: E. coli catabolite activator protein note: CAP binding activates transcription in the presence of cAMP."
     rep_origin      complement(1535..2120)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: LEFT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2291..3082)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /translation="HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     CDS             complement(3083..3151)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA"
     promoter        complement(3152..3256)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: bla"
     promoter        3532..3728
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: SV40 early promoter"
     rep_origin      3579..3714
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="note: SV40 origin of replication"
     regulatory      3754..3763
                     /label="Kozak sequence"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: vertebrate consensus sequence for strong initiation of translation (Kozak, 1987) regulatory_class: other: "
     misc_feature    3760..3822
                     /label="Igk secretion signal"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     CDS             3829..3855
                     /label="HA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="codon_start: 1 product: HA (human influenza hemagglutinin) epitope tag transl_table: 1 translation: YPYDVPDYA"
                     /translation="YPYDVPDYA"
     CDS             3862..3876
                     /label="Inserted_linker"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="codon_start: 1 transl_table: 1 translation: SGPGS"
                     /translation="SGPGS"
     CDS             3877..3888
                     /label="Consensus N-terminus"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="codon_start: 1 transl_table: 1 translation: SPED"
                     /translation="SPED"
     CDS             3889..3972
                     /label="A CC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="codon_start: 1 transl_table: 1 translation: EIQQLEEEIAQLEQKNAALKEKNQALKY"
                     /translation="EIQQLEEEIAQLEQKNAALKEKNQALKY"
     misc_feature    3973..4716
                     /label="EpoR-D1_D2_TM"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    4726..5556
                     /label="IL6ST"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     polyA_signal    5610..5834
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: bovine growth hormone polyadenylation signal"
     rep_origin      5880..6308
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: RIGHT note: f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
     promoter        6322..6651
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: SV40 enhancer and early promoter"
     rep_origin      6502..6637
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="note: SV40 origin of replication"
     CDS             6718..789
                     /label="NeoR/KanR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: aph(3')-II (or nptII) note: confers resistance to neomycin, kanamycin, and G418 (Geneticin®) product: aminoglycoside phosphotransferase from Tn5 transl_table: 1 translation: MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
ORIGIN
        1 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
       61 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
      121 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
      181 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
      241 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
      301 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
      361 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
      421 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
      481 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
      541 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
      601 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
      661 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
      721 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
      781 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
      841 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
      901 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
      961 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     1021 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     1081 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     1141 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     1201 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     1261 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     1321 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     1381 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     1441 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     1501 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     1561 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     1621 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     1681 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     1741 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     1801 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     1861 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     1921 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     1981 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     2041 gatccggcaa acaaaccacc gctggtagcg gtttttttgt ttgcaagcag cagattacgc
     2101 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     2161 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     2221 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     2281 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     2341 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     2401 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     2461 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     2521 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     2581 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     2641 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     2701 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     2761 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     2821 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     2881 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     2941 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     3001 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     3061 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     3121 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     3181 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     3241 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
     3301 atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     3361 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta
     3421 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
     3481 ttttgcgctg cttcgcgatg tacgggccag atatacgcgt gatctgcgat ctgcatctca
     3541 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     3601 gttccgccca ttctccgccc catcgctgac taattttttt tatttatgca gaggccgagg
     3661 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     3721 tttgcaaaaa gcttcgaatc gcgctagcgg ccggccacca tggaaactga tactttgctg
     3781 ctctgggtcc tgctgctgtg ggtccctgga tcaacggggg acgGATCCTA CCCATACGAT
     3841 GTTCCAGATT ACGCTGGTAC CTCCGGCCCT GGCAGCAGCC CCGAGGACGA GATCCAGCAA
     3901 CTGGAAGAGG AAATCGCCCA GCTGGAGCAG AAAAACGCCG CTCTGAAGGA AAAGAACCAG
     3961 GCCCTGAAGT ACgcaccttc acccagcctc ccggacccca agtttgagag caaagcggcc
     4021 ctgctggcat cccggggctc cgaagaactt ctgtgcttca cccaacgctt ggaagacttg
     4081 gtgtgtttct gggaggaagc ggcgagctcc gggatggact tcaactacag cttctcatac
     4141 cagctcgagg gtgagtcacg aaagtcatgt agcctgcacc aggctcccac cgtccgcggc
     4201 tccgtgcgtt tctggtgttc actgccaaca gcggacacat cgagtgctgt gccgctggag
     4261 ctgcaggtga cggaggcgtc cggttctcct cgctatcacc gcatcatcca tatcaatgaa
     4321 gtagtgctcc tggacgcccc cgcggggctg ctggcgcgcc gggcagaaga gggcagccac
     4381 gtggtgctgc gctggctgcc acctcctgga gcacctatga ccacccacat ccgatatgaa
     4441 gtggacgtgt cggcaggcaa ccgggcagga gggacacaaa gggtggaggt cctggaaggc
     4501 cgcactgagt gtgttctgag caacctgcgg ggcgggacgc gctacacctt cgctgttcga
     4561 gcgcgcatgg ccgagccgag cttcagcgga ttctggagtg cctggtctga gcccgcgtca
     4621 ctactgaccg ctagcgacct ggaccctctc atcttgacgc tgtctctcat tctggtcctc
     4681 atctcgttgt tgctgacggt tctggccctg ctgtccgcag cagcaaataa gcgagaccta
     4741 attaaaaaac acatctggcc taatgttcca gatccttcaa agagtcatat tgcccagtgg
     4801 tcacctcaca ctcctccaag gcacaatttt aattcaaaag atcaaatgta ttcagatggc
     4861 aatttcactg atgtaagtgt tgtggaaata gaagcaaatg acaaaaagcc ttttccagaa
     4921 gatctgaaat cattggacct gttcaaaaag gaaaaaatta atactgaagg acacagcagt
     4981 ggtattgggg ggtcttcatg catgtcatct tctaggccaa gcatttctag cagtgatgaa
     5041 aatgaatctt cacaaaacac ttcgagcact gtccaggctt ctaccgtggt acacagtggc
     5101 tacagacacc aagttccgtc agtccaagtc ttctcaagat ccgagtctac ccagcccttg
     5161 ttagattcag aggagcggcc agaagatcta caattagtag atcatgtaga tggcggtgat
     5221 ggtattttgc ccaggcaaca gtacttcaaa cagaactgca gtcagcatga atccagtcca
     5281 gatatttcac attttgaaag gtcaaagcaa gtttcatcag tcaatgagga agattttgtt
     5341 agacttaaac agcagatttc agatcatatt tcacaatcct gtggatctgg gcaaatgaaa
     5401 atgtttcagg aagtttctgc agcagatgct tttggtccag gtactgaggg acaagtagaa
     5461 agatttgaaa cagttggcat ggaggctgcg actgatgaag gcatgcctaa aagttactta
     5521 ccacagactg tacggcaagg cggctacatg cctcagtagg cggccgctcg agtctagagg
     5581 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     5641 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     5701 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     5761 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     5821 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     5881 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     5941 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     6001 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     6061 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     6121 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     6181 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     6241 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     6301 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     6361 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     6421 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     6481 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     6541 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     6601 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     6661 ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg
     6721 att
//