LOCUS       pLeo-B-α2-GEMS(JAK-STAT 6753 bp ds-DNA     circular     12-SEP-2023
DEFINITION  .
KEYWORDS    "creator:Trial User" "marker:AmpR, NeoR/KanR"
COMMENT     Imported using the Genbank importer. File name: pleo-b-a2-gems.gb
FEATURES             Location/Qualifiers
     polyA_signal    963..1084
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: SV40 polyadenylation signal"
     primer_bind     complement(1133..1149)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
     protein_bind    1157..1173
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: lac repressor encoded by lacI note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1181..1187)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(1188..1205)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(1206..1211)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     protein_bind    1226..1247
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: E. coli catabolite activator protein note: CAP binding activates transcription in the presence of cAMP."
     rep_origin      complement(1535..2120)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: LEFT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2291..3082)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /translation="HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     CDS             complement(3083..3151)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA"
     promoter        complement(3152..3256)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: bla"
     promoter        3532..3728
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: SV40 early promoter"
     rep_origin      3579..3714
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="note: SV40 origin of replication"
     regulatory      3754..3763
                     /label="Kozak sequence"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: vertebrate consensus sequence for strong initiation of translation (Kozak, 1987) regulatory_class: other: "
     misc_feature    3760..3822
                     /label="Igk secretion signal"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     CDS             3829..3855
                     /label="HA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="codon_start: 1 product: HA (human influenza hemagglutinin) epitope tag transl_table: 1 translation: YPYDVPDYA"
                     /translation="YPYDVPDYA"
     CDS             3862..3876
                     /label="Inserted_linker"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="codon_start: 1 transl_table: 1 translation: SGPGS"
                     /translation="SGPGS"
     CDS             3877..3888
                     /label="Consensus_N-terminus"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="codon_start: 1 transl_table: 1 translation: SPED"
                     /translation="SPED"
     CDS             3889..3972
                     /label="B CC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="codon_start: 1 transl_table: 1 translation: ENAALEEKIAQLKQKNAALKEEIQALEY"
                     /translation="ENAALEEKIAQLKQKNAALKEEIQALEY"
     CDS             3973..3973
                     /label="Linker"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="codon_start: 1 transl_table: 1 translation: "
                     /translation=""
     misc_feature    3979..4002
                     /label="α2"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    4003..4746
                     /label="EpoR-D1_D2_TM"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    4756..5586
                     /label="IL6ST"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     polyA_signal    5640..5864
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: bovine growth hormone polyadenylation signal"
     rep_origin      5910..6338
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: RIGHT note: f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
     promoter        6352..6681
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: SV40 enhancer and early promoter"
     rep_origin      6532..6667
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="note: SV40 origin of replication"
     CDS             6748..789
                     /label="NeoR/KanR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: aph(3')-II (or nptII) note: confers resistance to neomycin, kanamycin, and G418 (Geneticin®) product: aminoglycoside phosphotransferase from Tn5 transl_table: 1 translation: MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
ORIGIN
        1 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
       61 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
      121 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
      181 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
      241 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
      301 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
      361 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
      421 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
      481 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
      541 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
      601 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
      661 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
      721 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
      781 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
      841 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
      901 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
      961 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     1021 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     1081 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     1141 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     1201 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     1261 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     1321 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     1381 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     1441 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     1501 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     1561 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     1621 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     1681 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     1741 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     1801 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     1861 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     1921 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     1981 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     2041 gatccggcaa acaaaccacc gctggtagcg gtttttttgt ttgcaagcag cagattacgc
     2101 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     2161 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     2221 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     2281 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     2341 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     2401 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     2461 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     2521 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     2581 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     2641 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     2701 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     2761 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     2821 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     2881 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     2941 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     3001 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     3061 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     3121 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     3181 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     3241 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
     3301 atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     3361 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta
     3421 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
     3481 ttttgcgctg cttcgcgatg tacgggccag atatacgcgt gatctgcgat ctgcatctca
     3541 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     3601 gttccgccca ttctccgccc catcgctgac taattttttt tatttatgca gaggccgagg
     3661 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     3721 tttgcaaaaa gcttcgaatc gcgctagcgg ccggccacca tggaaactga tactttgctg
     3781 ctctgggtcc tgctgctgtg ggtccctgga tcaacggggg acgGATCCTA CCCATACGAT
     3841 GTTCCAGATT ACGCTGGTAC CTCCGGCCCT GGCAGCAGCC CCGAGGACGA GAACGCCGCT
     3901 CTGGAAGAGA AGATCGCCCA GCTGAAACAG AAGAACGCCG CCCTGAAGGA AGAGATCCAG
     3961 GCCCTGGAAT ACGAATTCGG ATCAGGGTCT GGAAGTGGGA GTgcaccttc acccagcctc
     4021 ccggacccca agtttgagag caaagcggcc ctgctggcat cccggggctc cgaagaactt
     4081 ctgtgcttca cccaacgctt ggaagacttg gtgtgtttct gggaggaagc ggcgagctcc
     4141 gggatggact tcaactacag cttctcatac cagctcgagg gtgagtcacg aaagtcatgt
     4201 agcctgcacc aggctcccac cgtccgcggc tccgtgcgtt tctggtgttc actgccaaca
     4261 gcggacacat cgagtgctgt gccgctggag ctgcaggtga cggaggcgtc cggttctcct
     4321 cgctatcacc gcatcatcca tatcaatgaa gtagtgctcc tggacgcccc cgcggggctg
     4381 ctggcgcgcc gggcagaaga gggcagccac gtggtgctgc gctggctgcc acctcctgga
     4441 gcacctatga ccacccacat ccgatatgaa gtggacgtgt cggcaggcaa ccgggcagga
     4501 gggacacaaa gggtggaggt cctggaaggc cgcactgagt gtgttctgag caacctgcgg
     4561 ggcgggacgc gctacacctt cgctgttcga gcgcgcatgg ccgagccgag cttcagcgga
     4621 ttctggagtg cctggtctga gcccgcgtca ctactgaccg ctagcgacct ggaccctctc
     4681 atcttgacgc tgtctctcat tctggtcctc atctcgttgt tgctgacggt tctggccctg
     4741 ctgtccgcag cagcaaataa gcgagaccta attaaaaaac acatctggcc taatgttcca
     4801 gatccttcaa agagtcatat tgcccagtgg tcacctcaca ctcctccaag gcacaatttt
     4861 aattcaaaag atcaaatgta ttcagatggc aatttcactg atgtaagtgt tgtggaaata
     4921 gaagcaaatg acaaaaagcc ttttccagaa gatctgaaat cattggacct gttcaaaaag
     4981 gaaaaaatta atactgaagg acacagcagt ggtattgggg ggtcttcatg catgtcatct
     5041 tctaggccaa gcatttctag cagtgatgaa aatgaatctt cacaaaacac ttcgagcact
     5101 gtccaggctt ctaccgtggt acacagtggc tacagacacc aagttccgtc agtccaagtc
     5161 ttctcaagat ccgagtctac ccagcccttg ttagattcag aggagcggcc agaagatcta
     5221 caattagtag atcatgtaga tggcggtgat ggtattttgc ccaggcaaca gtacttcaaa
     5281 cagaactgca gtcagcatga atccagtcca gatatttcac attttgaaag gtcaaagcaa
     5341 gtttcatcag tcaatgagga agattttgtt agacttaaac agcagatttc agatcatatt
     5401 tcacaatcct gtggatctgg gcaaatgaaa atgtttcagg aagtttctgc agcagatgct
     5461 tttggtccag gtactgaggg acaagtagaa agatttgaaa cagttggcat ggaggctgcg
     5521 actgatgaag gcatgcctaa aagttactta ccacagactg tacggcaagg cggctacatg
     5581 cctcagtagg cggccgctcg agtctagagg gcccgtttaa acccgctgat cagcctcgac
     5641 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     5701 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     5761 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     5821 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     5881 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     5941 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     6001 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     6061 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     6121 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     6181 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     6241 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     6301 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     6361 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     6421 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     6481 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     6541 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     6601 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     6661 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     6721 caagagacag gatgaggatc gtttcgcatg att
//