LOCUS       FB376                  11106 bp ds-DNA     circular     23-AUG-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..4
                     /label="GTCA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    18..42
                     /label="RB"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(1104..2104)
                     /label="STA\region\from\pVS1\plasmid"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    2697..3697
                     /label="pVS1-Rep\ori"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    complement(4107..4367)
                     /label="pBR322"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     rep_origin      complement(4507..4787)
                     /label="pBR322\ori"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             complement(5079..6089)
                     /label="SpmR"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     promoter        complement(6090..6223)
                     /label="Spm\Promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    6656..6681
                     /label="LB"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     Terminador      complement(6707..6951)
                     /label="TtrpC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             complement(6959..7687)
                     /label="YFP"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="vntifkey: 4"
     mutation        complement(7577..7577)
                     /label="T>C"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="vntifkey: 62"
     misc_feature    complement(7689..8416)
                     /label="PgpdA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    8429..9544
                     /label="Prom_pyr4"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="ApEinfo_revcolor: #f58a5e"
     misc_feature    9549..10694
                     /label="CDS_pyr4"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="ApEinfo_revcolor: #ff9ccd"
     misc_feature    10699..11102
                     /label="Tpyr4"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="ApEinfo_revcolor: #b1ff67"
ORIGIN
        1 gtcatgagac cggatcctga caggatatat tggcgggtaa acctaagaga aaagagcgtt
       61 tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta
      121 tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca acccctccgc
      181 tgctatagtg cagtcggctt ctgacgttca gtgcagccgt catctgaaaa cgacatgtcg
      241 cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc
      301 gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg
      361 aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac
      421 ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag
      481 atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct
      541 ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg
      601 gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg
      661 gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag
      721 ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcccga
      781 ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag
      841 ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc
      901 tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg
      961 cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat
     1021 gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca
     1081 ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc
     1141 acctctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg
     1201 cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg
     1261 tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatccga tgagtaaata
     1321 aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc
     1381 aggcaagacg accatcggaa cccatctagc ccgcgccctg caactcgccg gggccgatgt
     1441 tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga
     1501 tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat
     1561 cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc
     1621 cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg
     1681 ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct
     1741 acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc
     1801 cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag
     1861 ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc
     1921 tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga
     1981 ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc
     2041 agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac
     2101 tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag
     2161 accattaccg agctgctatc tgaatagatc gcgcagctac cagagtaaat gagcaaatga
     2221 ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc
     2281 aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag
     2341 cggctgggtt gtctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc
     2401 gtgacggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg
     2461 tggagaagtt gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc
     2521 ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg
     2581 cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg
     2641 ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt
     2701 tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg
     2761 ggcacgtaga ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg
     2821 tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag
     2881 acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag
     2941 ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc
     3001 acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg
     3061 gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca
     3121 tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg
     3181 tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc
     3241 gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg
     3301 aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg
     3361 ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc
     3421 tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg
     3481 agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaagga ctctttcctg
     3541 tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg
     3601 ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga
     3661 aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc
     3721 tggcctgtgc ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc
     3781 ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc
     3841 gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt
     3901 cgccactcga ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac
     3961 ggtgaaaacc tctgacacat gcagctcccg gtgacggtca cagcttgtct gtaagcggat
     4021 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca
     4081 gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag
     4141 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     4201 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     4261 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     4321 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     4381 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     4441 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     4501 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     4561 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     4621 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     4681 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     4741 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     4801 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     4861 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     4921 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     4981 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     5041 actcacgtta agggattttg gtcatgcatt ctaggtgatt atttgccgac taccttggtg
     5101 atctcgcctt tcacgtagtg gacaaattct tccaactgat ctgcgcgcga ggccaagcga
     5161 tcttcttctt gtccaagata agcctgtcta gcttcaagta tgacgggctg atactgggcc
     5221 ggcaggcgct ccattgccca gtcggcagcg acatccttcg gcgcgatttt gccggttact
     5281 gcgctgtacc aaatgcggga caacgtaagc actacatttc gctcatcacc agcccagtcg
     5341 ggcggcgagt tccatagcgt taaggtttca tttagcgcct caaatagatc ctgttcagga
     5401 accggatcaa agagttcctc cgccgctgga cctaccaagg caacgctatg ttctcttgct
     5461 tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat acctgcaaga
     5521 atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga
     5581 atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc gctctctcca
     5641 ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc
     5701 cttacggtca ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc gccatccact
     5761 gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc gatgacgcca
     5821 actacctctg atagttgagt cgatacttcg gcgatcaccg cttccctcat aatgtttaac
     5881 tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat
     5941 cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg taccccaaaa
     6001 aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc
     6061 aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagctt acgaaccgaa
     6121 caggcttatg tccactgggt tcgtgccttc atccgtttcc acggtgtgcg tcacccggca
     6181 accttgggta gcagcgaagt cgaggcattt ctgtcctggc tggaacagaa cttattattt
     6241 ccttcctctt ttctacagta tttaaagata ccccaagaag ctaattataa caagacgaac
     6301 tccaattcac tgttccttgc attctaaaac cttaaatacc agaaaacagc tttttcaaag
     6361 ttgttttcaa agttggcgta taacatagta tcgacggagc cgattttgaa accgcggtga
     6421 tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc
     6481 cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca
     6541 aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg
     6601 tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca
     6661 ggatatattg tggtgtaaac ataacggatc cggtctcagg agagcgcatg tctcagacgg
     6721 tcgatgcatt gcagatgagc tgtatctgga agaggtaaac ccgaaacgcg ttttattctt
     6781 gttgacatgg agctattaaa tcactagaag gcactctttg ctgcttggac aaatgaacgt
     6841 atcttatcga gatcctgaac accatttgtc tcaactccgg agctgacatc gacaccaacg
     6901 atcttatatc cagattcgtc aagctgtttg atgatttcag taacgttaag taagctcagc
     6961 tcatgactga cttgtagagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
     7021 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactggtagc
     7081 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
     7141 agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct
     7201 tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact
     7261 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc
     7321 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt
     7381 ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt
     7441 cgtgctgctt catgtggtcg gggtagcggg cgaagcactt caggccgtag ccgaaggtgg
     7501 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca
     7561 gcttgccgta ggtggcgtcg ccctcgccct cgccggacac gctgaacttg tggccgttta
     7621 cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc
     7681 tcaccatttt aaaggttctt ggatgggaag atgaatatac tgaagatggg aaaagaaaga
     7741 gaaaagaaaa gagcagctgg tggggagagc aggaaaatat ggcaacaaat gttggactga
     7801 cgcaacgacc ttgtcaaccc cgccgacaca ccgggcggac agacggggca aagctgccta
     7861 ccagggactg agggacctca gcaggtcgag tgcagagcac cggatgggtc gactgccagc
     7921 ttgtgttccc ggtctgcgcc gctggccagc tcctgagcgg cctttccggt ttcatacacc
     7981 gggcaaagca ggagaggcac gatatttgga cgccctacag atgccggatg ggccaattag
     8041 ggagcttacg cgccgggtac tcgctctacc tacttcggag aaggtactat ctcgtgaatc
     8101 ttttaccaga tcggaagcaa ttggacttct gtacctaggt taatggcatg ctatttcgcc
     8161 gacggctata cacccctggc ttcacattct ccttcgctta ctgccggtga ttcgatgaag
     8221 ctccatattc tccgatgatg caatagattc ttggtcaacg aggggcacac cagcctttcc
     8281 acttcggggc ggaggggcgg ccggtcccgg attaataatc atccactgca cctcagagcc
     8341 gccagagctg tctggcgcag tggcgcttat tactcagccc ttctctctgc gtccgtccgt
     8401 ctgtccgcat gcgccactcc gtcaggagga tctgtgtctt ttgccttgcc ttgtcttgtc
     8461 tgggttcctg ctcgtctgcg gcaatcggaa cgccgccagt gcggtgccaa gcaaaccagc
     8521 caaggtaggt acctaccact aggcttcttt tctcgttgtc tcactctctc ttttcctctt
     8581 tgtcctctct tatccccatc ttttctctct ctctctgctc ctttcctaac cacttcccta
     8641 cctttctctt tttccttttc ttgtcatctc catcttggct gacgaaaaag gtctgactgg
     8701 gtaggtatta tctggcagac ttgtgtgtat cattcaccct atttctgctt catagtacat
     8761 gtactgtacc tgaacggctc aaccgctatt tacgactctt atttttttgt ggcgttggtc
     8821 acgtttgcca gctgttgtcc gtctttctag ggctcctcaa acttgacctg accgagctcc
     8881 ctttctggac ccggtgggct tcacttccag ctgctgagcg acctgagccg aacatcctca
     8941 gtccttgtcc agcgcaattc attttctttc cttttctttt tttttattcc tttctttact
     9001 tttattctct ctttttctcc tcttcctctt cttcttcttt ctcctcctcc tccatatcct
     9061 cactctcgtt tccctcatta ctaccctctc ggctcctcag gtccaccaac cctcccgcac
     9121 ccaaacctct gccgctgaaa cccattcggt ggtcgccgtt tttttttttt tttttttctc
     9181 acccccaaag tcgcaatatc gggtatcgcc gccggcattg aatcgccttc tccgctagca
     9241 tcgactactg ctgctctgct ctcgttgcca gcgctgctcc ctagaatttt gaccagggga
     9301 cgagcccgac attaaagcaa ctccctcgcc tcgagatgac tcggatcgca cgaaattctc
     9361 ccaatcgccg acagttccta ctcctcttcc tcccgcacgg ctgtcgcgct tccaacgtca
     9421 ttcgcacagc agaattgtgc catctctctc ttttttttcc ccccctctaa accgccacaa
     9481 cggcacccta agggttaaac tatccaacca gccgcagcct cagcctctct cagcctcatc
     9541 agccaatgat ggcaccacac ccgacgctca aggccacctt cgcggccagg agcgagactg
     9601 cgacgcaccc gctgacggct tacctgttca agctcatgga cctcaaggca tccaacctgt
     9661 gcctgagcgc cgacgtgccg acagcgcgcg agctgctgta cctggccgac aagattggtc
     9721 cgtcgattgt cgtgctcaag acgcactacg acatggtttc gggctgggac ttccacccgg
     9781 agactggcac gggagcccag ctggcgtcgc tggctcgcaa gcacggcttc ctcatcttcg
     9841 aggaccgcaa gtttggtgac attggccaca ccgtcgagct gcagtacacg ggtgggtcgg
     9901 ctcgcatcat cgactgggct cacattgtca acgtcaacat ggtgcccggt aaggcgtcgg
     9961 tggcctcgct ggcccagggt gccaagcgct ggctcgagcg ctacccctgc gaggtcaaga
    10021 cgtccgtcac cgtcggcacg cccaccatgg actcgtttga cgacgacgcc gactccaggg
    10081 acgccgagcc agccggtgcc gtcaacggta tgggttccat tggtgtcctg gacaagccca
    10141 tctactcgaa ccggtccggc gacggccgca agggaagcat cgtttccatc accaccgtca
    10201 cccagcagta cgagtccgtt tcctcgccac ggttaacaaa ggccatcgcc gagggtgacg
    10261 agtcgctctt cccgggaatc gaggaggctc cgctgagccg cggcctcctg atcctcgccc
    10321 aaatgtccag ccagggcaac ttcatgaaca aggagtacac gcaggcctgc gtcgaggccg
    10381 cccgggagca caaggacttt gtcatgggct tcatctcgca ggagactctc aacaccgagc
    10441 ccgacgatgc ctttatccac atgacgcccg gctgccagct gccccccgaa gacgaggacc
    10501 agcagaccaa cggatcggtc ggtggagatg gccagggcca gcagtacaac acgccgcaca
    10561 agctgattgg catcgccggc agcgacattg ccattgtggg ccggggcatc ctcaaggcct
    10621 cagaccccgt agaggaggca gagcggtacc gatcagcagc gtggaaagcc tacaccgaga
    10681 ggctgctgcg ataggcttgg gagggaaggg aagaaagaag taaagaaagg catttagcaa
    10741 gaagggggaa aagggaggga ggacaaacgg agctgagaaa gagctcttgt ccaaagcccg
    10801 gcatcataga atgcagctgt atttaggcga cctctttttc catcttgtcg atttttgtta
    10861 tgacgtacca gttgggatga tggatgattg taccccagct gcgattgatg tgtatctttg
    10921 catgcaacaa cacgcgatgg cggaggcgaa ctgcacattg gaaggttcat atatggtcct
    10981 gacatatctg gtggatctgg aagcatggaa ttgtattttt gatttggcat ttgcttttgc
    11041 gcgtggaggg aacatatcac cctcgggcat ttttcatttg gtaggatggt ttggatgcag
    11101 ttcgct
//