LOCUS       J1219_pENTR4_AvrRpm1_CDS_no_stop        2909 bp    DNA     circular UNA 15-SEP-2023
DEFINITION  A new nucleotide sequence entered manually.
ACCESSION   urn.local...c-grac5w8
VERSION     urn.local...c-grac5w8
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     terminator      767..810
                     /note="Geneious type: Terminator"
     terminator      942..969
                     /note="Geneious type: Terminator"
     misc_feature    1019..1115
                     /note="Geneious type: attL1"
                     /standard_name="attL1"
     CDS             1123..1782
                     /standard_name="CDS AvrRpm1"
     misc_feature    complement(1802..1898)
                     /note="Geneious type: attL2"
                     /standard_name="attL2"
     misc_feature    2021..2830
                     /note="Geneious type: Marker"
                     /standard_name="kan2 marker"
     misc_feature    2904..614
                     /note="Geneious type: Origin of Replication"
                     /standard_name="pBR322 origin"
ORIGIN      
        1 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
       61 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
      121 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
      181 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
      241 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
      301 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
      361 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
      421 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
      481 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
      541 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
      601 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
      661 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgctagc atggatctcg
      721 gggacgtcta actactaagc gagagtaggg aactgccagg catcaaataa aacgaaaggc
      781 tcagtcggaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag
      841 taggacaaat ccgccgggag cggatttgaa cgttgtgaag caacggcccg gagggtggcg
      901 ggcaggacgc ccgccataaa ctgccaggca tcaaactaag cagaaggcca tcctgacgga
      961 tggccttttt gcgtttctac aaactcttcc tgttagttag ttacttaagc tcgggcccca
     1021 aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg ataagcaatg
     1081 cttttttata atgccaactt tgtacaaaaa agcaggctcc acatgggctg tgtatcgagc
     1141 acttcaagaa gtactggata ttattccggt tacgaaaatc atgaggagcc aagggtggca
     1201 agtagcccga cgaacaggca tgatcgcggc tacgaaaccg attccgagca ttccaatgtt
     1261 aacttaatac ccaatgcgag gagagtgtat aaagagtctg ttttgtggca tggtacatct
     1321 atgcaatcaa aaatagattt gagaaggcaa gggtttgatg tgaatagaaa aactgatggt
     1381 gcaaccgagg gatctagaac atcgaactca gcccctacag acacgctcat cagaaatgct
     1441 agacgtcaca actacttaac agcgtatgat gtaacagcta agaattatgc tcgaagagca
     1501 gaccccgaat ccccagccct agttagaact ataggtgtca agagcaattt taatattgag
     1561 ctagatcctg aatcgaaaga cgagaatggt gaaatatccc aattcagctg cagaacgaca
     1621 gattcaatac ctaaaaaata cattgttgga agcaagagca gccagccggg agcagatgca
     1681 aaggttttta aaaaagaact ctccaatgcg ggctttgaag tttccacaag ccgtgcaggc
     1741 gagttattaa gagctgttca gtctgactca gaagatgact ttaactcgag atatctagac
     1801 ccagctttct tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac
     1861 gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctgc agctctggcc
     1921 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat
     1981 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga
     2041 aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
     2101 tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc
     2161 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
     2221 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
     2281 tactcctggt gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt
     2341 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
     2401 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct
     2461 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
     2521 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc
     2581 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
     2641 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct
     2701 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca
     2761 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga
     2821 gtttttctaa tcagaattgg ttaattggtt gtaacattat tcagattggg ccccgttcca
     2881 ctgagcgtca gaccccgtag aaaagatca
//
