LOCUS       pBJ089_zACNE20_cmlc2_V 10792 bp ds-DNA     circular     11-MAR-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          1..20
                     /label="pBJ088_zACNE20_cmlc2_Voltron_P2A_CoChR_GFP FWD"
                     /note="sequence: ATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     rep_origin      complement(64..746)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             complement(844..1503)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     rep_origin      complement(1964..2270)
                     /label="F1 ori"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             complement(2289..2357)
                     /label="LacZ alpha"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     2428..2445
                     /label="M13-fwd"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          2429..2444
                     /label="GENEWIZ UNIVERSAL M13F"
                     /note="sequence: gtaaaacgacggccag"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     2454..2474
                     /label="T7"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          2455..2474
                     /label="GENEWIZ UNIVERSAL T7"
                     /note="sequence: TAATACGACTCACTATAGGG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          4323..4342
                     /label="sprBJ043"
                     /note="sequence: CAGAGGTGTAAAAAGTACTC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    4816..4837
                     /label="Sequence_FP1"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    4864..4869
                     /label="XhoI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    4877..4884
                     /label="Gateway Cassette"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     misc_feature    4877..4884
                     /label="attR1"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_fwdcolor="#0000ff"
     misc_feature    4877..4884
                     /label="Frag4-F3"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    4877..4884
                     /label="Frag4-F2"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    4904..4935
                     /label="FP"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    4906..5594
                     /label="ATAC_diff_peaks"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     misc_feature    5247..5254
                     /label="GATA5 motif"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    5420..5438
                     /label="GATA1 motif"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    5615..5642
                     /label="RP"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    5654..5668
                     /label="Gateway Cassette"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     misc_feature    5676..5681
                     /label="BglII"
                     /ApEinfo_revcolor="#008080"
                     /ApEinfo_fwdcolor="#008080"
     promoter        5683..6581
                     /label="cmlc2 promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(5683..6581)
                     /label="XT103 pDestTol2CG2 extraction (concatenatedsequence 2)"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          5736..5755
                     /label="sprBJ051"
                     /note="sequence: gctctccaaatcagcagact"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(5934..5939)
                     /label="BsrGI"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(6036..6055)
                     /label="sprBJ050"
                     /note="sequence: gaatgaagcaaacagcagcc"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    complement(6349..6356)
                     /label="MZF"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(6356..6364)
                     /label="GATA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(6367..6373)
                     /label="Nkx2.5"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    complement(6403..6409)
                     /label="nkx2.5"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    complement(6411..6419)
                     /label="GATA"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    6454..6461
                     /label="MZF"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    complement(6474..6479)
                     /label="PstI"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    6482..6488
                     /label="Cdx"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    6499..6504
                     /label="GATA"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             6582..7262
                     /label="Ace2N"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             6582..9551
                     /label="Translation 6582-9551"
                     /translation="MADVETETGMIAQWIVFAIMAAAAIAFGVAVHFRPSELKSAYYINIAICTIAATAYYAMAVNYQDLTMNGERQVVYARYINWVLTTPLLLLDLIVMTKMGGVMISWVIGADIFMIVFGILGAFEDEHKFKWVYFIAGCVMQAVLTYGMYNATWKDDLKKSPEYHSSYVSLLVFLSILWVFYPVVWAFGSGSGVLSVDNEAILMGILDVLAKPLFGMGCLIAHETIFKIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLEISGEPTTKSRITSEGEYIPLDQIDINVFCYENEVGSGATNFSLLKQAGDVEENPGPVDSSRRKWNKTGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKELFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK*"
     primer          7070..7090
                     /label="sprBJ045"
                     /note="sequence: ccatagctcctatgtcagtct"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(7170..7189)
                     /label="sprBJ052"
                     /note="sequence: atgagaatggcctcattgtc"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             7263..8156
                     /label="HaloTag"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          7800..7819
                     /label="sprBJ053"
                     /note="sequence: gaagtcgagatggaccatta"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             8157..8216
                     /label="KGC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             8217..8237
                     /label="ER2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(8218..8237)
                     /label="pBJ088_zACNE20_cmlc2_Voltron_P2A_CoChR_GFP REV"
                     /note="sequence: aagttcgtggctccggagccCACCTCGTTCTCGTAACAGA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(8218..8247)
                     /label="prBJ205"
                     /note="sequence: ctccggagccCACCTCGTTCTCGTAACAGA"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          8228..8253
                     /label="prBJ202"
                     /note="sequence: GAACGAGGTGggctccggagccacga"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          8238..8257
                     /label="pBJ065_zACNE20_memCherry_P2A_jGCaMP7f FWD"
                     /note="sequence: TCTGTTACGAGAACGAGGTGggctccggagccacgaactt"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             8238..8303
                     /label="P2A"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     regulatory      8304..8304
                     /label="Kozak sequence"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             8316..8372
                     /label="calmodulin-binding peptide"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          8398..8417
                     /label="sprBJ006"
                     /note="sequence: acaagcagaagaacggcatc"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(8579..8596)
                     /label="sprBJ005"
                     /note="sequence: AGCAGGACCATGTGATCG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(8630..8647)
                     /label="prBJ145"
                     /note="sequence: ttgtacagctcgtccatg"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(8712..8733)
                     /label="GENEWIZ UNIVERSAL EGFP-N"
                     /note="sequence: cgtcgccgtccagctcgaccag"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    9549..9551
                     /label="151013 bactin1321 sequencing verified"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    9549..9551
                     /label="151013 SV40_132_rev sequencing verified"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal    9580..9771
                     /label="SV40 late polyA"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     repeat_region   complement(10147..10346)
                     /label="Tol2-L200"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          10767..10792
                     /label="prBJ066"
                     /note="sequence: GCGGTAATACGGTTATCCACAGAATC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(10767..10792)
                     /label="pBJ065_zACNE20_memCherry_P2A_jGCaMP7f REV"
                     /note="sequence: TCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(10767..10791)
                     /label="prBJ065"
                     /note="sequence: ATTCTGTGGATAACCGTATTACCGC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
ORIGIN
        1 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA
       61 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA
      121 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
      181 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
      241 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG
      301 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA
      361 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC
      421 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
      481 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG
      541 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA
      601 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA
      661 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA
      721 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT
      781 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG
      841 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT
      901 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC
      961 CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA
     1021 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA
     1081 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA
     1141 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT
     1201 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC
     1261 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT
     1321 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC
     1381 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG
     1441 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT
     1501 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC
     1561 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG
     1621 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC
     1681 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG
     1741 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT
     1801 TCCGCGCACA TTTCCCCGAA AAGTGCCACC TAAATTGTAA GCGTTAATAT TTTGTTAAAA
     1861 TTCGCGTTAA ATTTTTGTTA AATCAGCTCA TTTTTTAACC AATAGGCCGA AATCGGCAAA
     1921 ATCCCTTATA AATCAAAAGA ATAGACCGAG ATAGGGTTGA GTGTTGTTCC AGTTTGGAAC
     1981 AAGAGTCCAC TATTAAAGAA CGTGGACTCC AACGTCAAAG GGCGAAAAAC CGTCTATCAG
     2041 GGCGATGGCC CACTACGTGA ACCATCACCC TAATCAAGTT TTTTGGGGTC GAGGTGCCGT
     2101 AAAGCACTAA ATCGGAACCC TAAAGGGAGC CCCCGATTTA GAGCTTGACG GGGAAAGCCG
     2161 GCGAACGTGG CGAGAAAGGA AGGGAAGAAA GCGAAAGGAG CGGGCGCTAG GGCGCTGGCA
     2221 AGTGTAGCGG TCACGCTGCG CGTAACCACC ACACCCGCCG CGCTTAATGC GCCGCTACAG
     2281 GGCGCGTCCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC
     2341 TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA
     2401 ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAGCG CGCGTAATAC
     2461 GACTCACTAT AGGGCGAATT GGGTACCAAA TAGTAGGAAT TACCCACCTG TACAAGTGCT
     2521 GAAAACTTGG ATGAATAAGC CCGTTTGCCA TTTTCTACTG CTATTTTAAA TCTTTTCTGT
     2581 ATTTGTCTGC ATTTGTCTTT ACCCTTGAAT AAATGTTTTA GTTGTTTTTT TTCAATTATG
     2641 ACTGTGTTTA ACAGACATTA TTACAATAAT ATGTAATAGT AGTACACACT ATTATTATGT
     2701 AATAGTACTT GTTGACTGTA TTTGAGAACT GGACCGAGTG AGTGTTACGT CACCCATTCA
     2761 AAATGACTTA CTTCTGGCTC CAACAAAATG AAGTTAATTC AGTTGCCATT TTTCACTGTA
     2821 TGGACATCGC CGTGTTGGAG CTAGACGTTG CCAATAAGCA AGAAAGAGCC GAGATGCGTC
     2881 GAGTCTGAGT CACCGTTCCT ATGGCAACCC CTCTAACCAA TCAGAAGTAA GCTTGTTGGA
     2941 AGTCCACAGC CTACCACTTG AAAGCGGGCT GCACAAAATC TGTCAAACGT TTTGAACGTT
     3001 GGATGTGAGA GCACATACTT TTATTAAGGC ATCTGGTTGG TCAGTTTATA ATATCAACAA
     3061 CTTGGGCTAC AGAAAAGAAA AGTTATTACA GAAATTATGA TTAACAAGTA CATGTTAAAT
     3121 AAAGATTTTA ATATGAATGC CACCACTGGA GCATTCATGC CATTTGGAGC TTCTTCCTGT
     3181 TTGGATCACT AGAAGGAGGA GGTCACTCAT TACAGTTCTC ATATACAGTC GTTGGTTGGT
     3241 TGGTTGGTTG GTAGATTGAT TGATTGATTG ATTGATTGAT TGATTGATTG ATTGATTGAT
     3301 TGATTGATTG ATTGATTGAT TGATTGATTG GTAGTCAAAA TAAGAAATAA TTTCCACAGA
     3361 TTCATTACAG AAATGATTAA ATGCATACAT AAAAAACTGG GGGGGGGGGG ATACAACAAC
     3421 ACACTTAAGT CACATTTGCC TACGTAAAGA AAAGTAAAGA AAATCAATAG CTATATTTTA
     3481 CATCTCCTTT TTTTGCTGTC TTTTAATTAG CCTTGTTTTG CTGTTATCTT GATTGAAACT
     3541 GTAACTTCTT CACCTGCTTC TTTTCTTTGT AGGTTTTGCC AGCCCAGAAA CAGGCATGGC
     3601 TGTGCAAGGC CAGAGCACCA TGCACAATGC GCTGCATGTC TTCATGAACG GCTCAATGTC
     3661 CTCAGTCCAG GGCTCAGCCA ACGACCCCAT CTTCCTCCTT CACCATGCTT TCATTGACAG
     3721 GTAACAAACA CGTCATGACA TTAGACTGCA CAGTTTTTGA CAAAGTTCAT ACAATCTGTT
     3781 GTTTATAGCT GCTACAATTA GTGAAGTTTG TGAATGTACT TGGATGAGCA GCGAAAGATC
     3841 AATTGAGATC AATTGTTAGA GTTTGGTTGC CCTGCAGAGC AAAGAACAAA AAATAATCTG
     3901 GTGGCTTTAC TGCGTGAGGT TATTATTGGT GGAATAGAAA CACAAAACAT AATTGCATTT
     3961 ATTTGTTTAA TTTTTTATCT TATCTTAACT TTCATCTTGC ATATTTGTTT CTTACATCAT
     4021 TTCTAGCATC TTTGAGCGCT GGCTAAGAAC TCATCAGCCT CCCCGGTCCA TCTACCCACG
     4081 TACCAATGCA CCAATTGGCC ACAATGACGG CTACTACATG GTGCCATTCC TTCCTCTTTA
     4141 TAGGAATGGA GACTACCTCC TGTCCAACAA TGCTCTTGGA TACGAGTACG CCTACCTGTT
     4201 GGACCCAGGT CATTGCACAA CACCAGAAAT GCCCTCTGAT CTGCAAAAGA CGTGAATATC
     4261 TGTTCAGACA CCCATATCCA CTCTGTTCCA CACAGGTCAG AGGTTTGTCC AGGAGTTCTT
     4321 GACAGAGGTG TAAAAAGTAC TCAAAAATTT TACTCAAGTG AAAGTACAAG TACTTAGGGA
     4381 AAATTTTACT CAATTAAAAG TAAAAGTATC TGGCTAGAAT CTTACTTGAG TAAAAGTAAA
     4441 AAAGTACTCC ATTAAAATTG TACTTGAGTA TTAAGGAAGT AAAAGTAAAA GCAAGAAAGA
     4501 AAACTAGAGA TTCTTGTTTA AGCTTTTAAT CTCAAAAAAC ATTAAATGAA ATGCATACAA
     4561 GGTTTTATCC TGCTTTAGAA CTGTTTGTAT TTAATTATCA AACTATAAGA CAGACAATCT
     4621 AATGCCAGTA CACGCTACTC AAAGTTGTAA AACCTCAGAT TTAACTTCAG TAGAAGCTGA
     4681 TTCTCAAAAT TGTTAGTGTC AAGCCTAGCT CTTTTGGGGC TGAAAAGCAA TCCTGCAGTG
     4741 CTGAAAAGCC TCTCACAGGC AGCCGATGCG GGAAGAGGTG TATTAGTCTT GATAGAGAGG
     4801 CTGCAAATAG CAGGAAACGT GAGCAGAGAC TCCCTGGTGT CTGAAACACA GGCCAGATGG
     4861 GCCCTCGAGG ATATCAACAA GTTTGTACAA AAAAGCAGGC TCGcagagtg agaatgtaaa
     4921 tgttaaataC GTAGgttaag tttcggtatt gaagaggatt atggatgtag agtaagatct
     4981 tgcagaatat gtgcttcata actaccaata atcagccaat atcttaataa taggctggta
     5041 aAAGTGAGGA TTTGTCCTTA AACTATTGCC AGTTTTTAag ctattcaggc aaatctgcat
     5101 ttttagcacc attactctag tcttcagtgc cacatgatcc ttcagaaatc accccaatat
     5161 gTCAGCTTTT CGGTTTGCTG CTTAACATTG TTGTGGAAAC TATAAGCGAA ATGGGAACTC
     5221 CTGTGTCTCA CACACTCGCT GATTTGTGTT ATCAGTATTT TTTTGGGATG GTGGGTGGTG
     5281 GCGCTCAGGC CTGCTGTGGA GCCGCTGTCG GCCCACTCCC CCTCAAATGG AGCCAGGCCT
     5341 GCCTGATAAG CCACGTCCCA GGACTAGCAG GGAGCCAGGC CTATCTGAGC CCCTGAGTGA
     5401 CTGGGTGACA CGCTCTTCCA TGGCCCGAAC TGATAGACAG GGCACAGCTA TACGGCACAG
     5461 TGAGGAAGAG GAAGGATGGG ATAAGGATGC AATCAGCAGT TTTCCCAAAC TCTGAAAACC
     5521 TCTGGATTTG ATTAGGCATG TAGGATGGCA AATGTATTGA TGATTTAAGG GAAGATTCAG
     5581 CCAAAATTGA GTCATCTTGT ATCCACCCTT ACTTCAATCC AAACCTGTAT TACTTTCTAC
     5641 CACACCCAGC TTTCTTGTAC AAAGTGGTGA TATCAAGATC TCaaagctta aatcagttgt
     5701 gttaaataag agacattcaa aataaatgta aatgagctct ccaaatcagc agacttaaca
     5761 ttctttaaaa tgattgattc aatagtgata aaaatcaggc atagccagtt gtaactttag
     5821 ataaattaca gaaaatgtca aatacagaga accgattctt ttttatgata catccaagca
     5881 cacatttaac acaatccagg caaaccccga atttcacagt cacaagcact gtttgtacaa
     5941 gagctttgcc taaggacaca cagtctctat aagtccaggt cgttggtttc actcttattt
     6001 taaacatgtg acatttttcc tgccatcctg tcttaggctg ctgtttgctt cattccatgt
     6061 cacattaaat tcctcagtag caccttttac acacacagcc aatcttttcc agaaaattca
     6121 attgctttga agagataatg tgtgaacaaa tccatttaga aaaggaaaat taagaatttg
     6181 taaaatcatc tgtaaattgt tggcattctt ctgtatatga acatcacatc atttacaggt
     6241 aaaggtctgg tcattaatta tatgacaatt tactggtatt attttgtgaa aggggctatt
     6301 ttcaatgcgt tcatccatcc ttttcatccc tcaaatctct cattcacgtc cccctcccca
     6361 tctgcacact ttatctcatt ttccaccctg ctggaatctg agcacttgtg cagttatcag
     6421 ggctcctgta tttaggaggc tctgggtgtc catgtagggg acgaacagaa acactgcaga
     6481 cctttataga agaacaattg ataagagtcc tcatacataa agactccatt agtaagccag
     6541 tgacccagga gcccagacca acagcaaagc agacagtgac catggctgac gtggaaaccg
     6601 agaccggcat gattgcacag tggattgtct ttgctattat ggctgctgct gctattgctt
     6661 ttggagtggc tgtgcacttt cggccttcag agctgaagag cgcatactat atcaacattg
     6721 ccatctgcac tatcgccgct accgcttact atgcaatggc cgtgaactac caggacctga
     6781 caatgaatgg tgaaaggcag gtggtctacg caagatatat taactgggtg ctgaccacac
     6841 cactgctcct gctcgatctc atcgtcatga ccaagatggg cggagtgatg atttcttggg
     6901 tcatcggcgc agacattttc atgatcgtgt ttggtattct gggcgccttc gaggatgaac
     6961 acaagttcaa atgggtgtac tttatcgctg gatgtgtgat gcaggcagtc ctgacatacg
     7021 ggatgtataa cgccacttgg aaagacgatc tgaagaaaag ccccgagtac catagctcct
     7081 atgtcagtct gctcgtcttc ctgtcaatcc tctgggtgtt ttatcctgtc gtgtgggctt
     7141 tcgggtctgg tagtggcgtg ctgtccgtcg acaatgaggc cattctcatg ggaatcctgg
     7201 atgtgctcgc taagccactg tttggaatgg ggtgcctcat tgcccatgag actatcttca
     7261 agatcggtac tggctttcca ttcgaccccc attatgtgga agtcctgggc gagcgcatgc
     7321 actacgtcga tgttggtccg cgcgatggca cccctgtgct gttcctgcac ggtaacccga
     7381 cctcctccta cgtgtggcgc aacatcatcc cgcatgttgc accgacccat cgctgcattg
     7441 ctccagacct gatcggtatg ggcaaatccg acaaaccaga cctgggttat ttcttcgacg
     7501 accacgtccg cttcatggat gccttcatcg aagccctggg tctggaagag gtcgtcctgg
     7561 tcattcacga ctggggctcc gctctgggtt tccactgggc caagcgcaat ccagagcgcg
     7621 tcaaaggtat tgcatttatg gagttcatcc gccctatccc gacctgggac gaatggccag
     7681 aatttgcccg cgagaccttc caggccttcc gcaccaccga cgtcggccgc aagctgatca
     7741 tcgatcagaa cgtttttatc gagggtacgc tgccgatggg tgtcgtccgc ccgctgactg
     7801 aagtcgagat ggaccattac cgcgagccgt tcctgaatcc tgttgaccgc gagccactgt
     7861 ggcgcttccc aaacgagctg ccaatcgccg gtgagccagc gaacatcgtc gcgctggtcg
     7921 aagaatacat ggactggctg caccagtccc ctgtcccgaa gctgctgttc tggggcaccc
     7981 caggcgttct gatcccaccg gccgaagccg ctcgcctggc caaaagcctg cctaactgca
     8041 aggctgtgga catcggcccg ggtctgaatc tgctgcaaga agacaacccg gacctgatcg
     8101 gcagcgagat cgcgcgctgg ctgtcgacgc tcgagatttc cggcgagcca accactAAGA
     8161 GCAGAATCAC AAGCGAGGGA GAGTACATCC CTCTGGACCA GATCGACATC AACGTGTTCT
     8221 GTTACGAGAA CGAGGTGggc tccggagcca cgaacttctc tctgttaaag caagcaggag
     8281 acgtggaaga aaaccccggt cccgtcgact catcacgtcg taagtggaat aagacaggtc
     8341 acgcagtcag agctataggt cggctgagct cactcgagaa cgtctatatc aaggccgaca
     8401 agcagaagaa cggcatcaag gcgaacttca agatccgcca caacatcgag gacggcggcg
     8461 tgcagctcgc ctaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     8521 ccgacaacca ctacctgagc gtgcagtcca aactttcgaa agaccccaac gagaagcgcg
     8581 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     8641 tgtacaaggg cggtaccgga gggagcatgg tgagcaaggg cgaggagctg ttcaccgggg
     8701 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     8761 gcgagggtga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     8821 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     8881 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     8941 gctacatcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     9001 aggtgaagtt cgagggcgac accctggtga accgcatcga gcttaagggc atcgacttca
     9061 aggaggacgg caacatcctg gggcacaagc tggagtacaa cctgccggac caactgactg
     9121 aagagcagat cgcagaattt aaagagctgt tctccctatt tgacaaggac ggggatggga
     9181 caataacaac caaggagctg gggacggtga tgcggtctct ggggcagaac cccacagaag
     9241 cagagctgca ggacatgatc aatgaagtag atgccgacgg tgacggcaca atcgacttcc
     9301 ctgagttcct gacaatgatg gcaagaaaaa tgaaatacac agacagtgaa gaagaaatta
     9361 gagaagcgtt ccgtgtgttt gataaggatg gcaatggcta catcagtgca gcagagcttc
     9421 gccacgtgat gacaaacctt ggagagaagt taacagatga agaggttgat gaaatgatca
     9481 gggaagcaga catcgatggg gatggtcagg taaactacga agagtttgta caaatgatga
     9541 cagcgaagTG AAGCTCGAAT TAATTCATCG ATGATGATCC AGACATGATA AGATACATTG
     9601 ATGAGTTTGG ACAAACCACA ACTAGAATGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT
     9661 GTGATGCTAT TGCTTTATTT GTAACCATTA TAAGCTGCAA TAAACAAGTT AACAACAACA
     9721 ATTGCATTCA TTTTATGTTT CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT
     9781 AAAACCTCTA CAAATGTGGT ATGGCTGATT ATGATCCTCT AGATCAGATC CGAAGATACG
     9841 GCCACGGGTG CTCTTGATCC TGTGGCTGAT TTTGGACTGT GCTGCTCGCA GCTGCTGATG
     9901 AATCACATAC TTCCTCCATT TTCTTCCACT GATTGACTGT TATAATTTCC CTAATTTCCA
     9961 GGTCAAGGTG CTGTGCATTG TGGTAATAGA TGTGACATGA CGTCACTTCC AAAGGACCAA
    10021 TGAACATGTC TGACCAATTT CATATAATGT GAAAACGATT TTCATAGGCA GAATAAATAA
    10081 CATTTAAATT AAACTGGGCA TCAGCGCAAT TCAATTGGTT TGGTAATAGC AAGGGAAAAT
    10141 AGAATGAAGT GATCTCCAAA AAATAAGTAC TTTTTGACTG TAAATAAAAT TGTAAGGAGT
    10201 AAAAAGTACT TTTTTTTCTA AAAAAATGTA ATTAAGTAAA AGTAAAAGTA TTGATTTTTA
    10261 ATTGTACTCA AGTAAAGTAA AAATCCCCAA AAATAATACT TAAGTACAGT AATCAAGTAA
    10321 AATTACTCAA GTACTTTACA CCTCTGGTTC TTGACCCCCT ACCTTCAGCA AGCCCAGCAG
    10381 ATCCACTAGT TCTAGAGCGG CCGCCACCGC GGTGGAGCTC CAGCTTTTGT TCCCTTTAGT
    10441 GAGGGTTAAT TGCGCGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT
    10501 ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG
    10561 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG
    10621 GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC
    10681 GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC
    10741 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TC
//