LOCUS       CAG174_1_1              7572 bp ds-DNA     circular     23-APR-2023
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name:
            Cohen_b6z_1_CAG174_1_1_pLann.gbk Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     misc_feature    complement(381..969)
                     /label="WPRE"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     primer          972..1007
                     /label="CAG174_HindIII_Rev"
                     /note="sequence: cgataagcttttaggaaatctccagagtagacagcc"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             complement(982..984)
                     /label="StopCodon"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /translation="*"
     CDS             complement(985..1869)
                     /label="HaloTag®"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="99.3"
                     /fragment="False"
                     /other="CDS"
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLEIS"
     CDS             complement(1870..2754)
                     /label="GEM7"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /translation="MNDLMRDLAPISAKAWAEIETEARGTLTVTLAARKVVDFKGPLGWDASSVSLGRTEALAEEPKAAGSAAVVTVRKRAVQPLIELCVPFTLKRAELEAIARGASDADLDPVIEAARAIAIAEDRAVFHGFAAGGITGIGEASAEHALDLPADLADFPGVLVRALAVLRDRGVDGPYALVLGRTVYQQLMETTTPGGYPVLQHVRRLFEGPLIWAPGVDGAMLISQRGGDFELTVGRDFSIGYHDHDAQSVHLYLQESMTFRCLGPEAAVPLRGLSQAATKAGTGGGGSGGGSGGGS"
     primer          complement(2740..2771)
                     /label="CAG174_BamHI_Fwd"
                     /note="sequence: ctcgaggatccgccaccatgaacgacctgatg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    2755..2760
                     /label="Kozak Sequence"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    complement(2771..2788)
                     /label="MCS (fragment)"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="34.0"
                     /fragment="True"
                     /other="misc_feature"
     intron          complement(2849..3865)
                     /label="chimeric intron"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="intron"
     promoter        complement(3866..4140)
                     /label="chicken β-actin promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="98.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     enhancer        complement(4142..4445)
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     repeat_region   complement(4573..4600)
                     /label="AAV2 ITR (fragment)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
     repeat_region   complement(4629..4656)
                     /label="AAV2 ITR (fragment)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
     rep_origin      complement(4729..5317)
                     /label="ori"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(5488..6348)
                     /label="AmpR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(6349..6453)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             6548..6583
                     /label="rop (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
                     /translation="LARFGDDGENL*"
     misc_feature    6685..6734
                     /label="bom (fragment)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="35.5"
                     /fragment="True"
                     /other="misc_feature"
     rep_origin      complement(6735..7190)
                     /label="f1 ori"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="98.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     misc_feature    7209..7260
                     /label="bom (fragment)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     repeat_region   complement(7265..7405)
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="repeat_region"
     polyA_signal    complement(7445..349)
                     /label="hGH poly(A) signal"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
ORIGIN
        1 aaaaaaacaa aaattagctg agcctggtca tgcatgcctg gaatcccaac aactcgggag
       61 gctgaggcag gagaatcgct tgaacccagg aggcggagat tgcagtgagc caagattgtg
      121 ccactgcact ccagcttggt tcccaataga ccccgcaggc cctacaggtt gtcttcccaa
      181 cttgcccctt gctccatacc acccccctcc accccataat attatagaag gacacctagt
      241 cagacaaaat gatgcaactt aattttatta ggacaaggct ggtgggcact ggagtggcaa
      301 cttccagggc caggagaggc actggggagg ggtcacaggg atgccacccg tagatctctc
      361 gagcagcgct cggtatcgat gcggggaggc ggcccaaagg gagatccgac tcgtctgagg
      421 gcgaaggcga agacgcggaa gaggccgcag agccggcagc aggccgcggg aaggaaggtc
      481 cgctggattg agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa tccaggtggc
      541 aacataggcg agcagccaag gaaaggacga tgatttcccc gacaacacca cggaattgtc
      601 agtgcccaac agccgagccc ctgtccagca gcgggcaagg caggcggcga tgagttccgc
      661 cgtggcaata gggaggggga aagcgaaagt cccggaaagg agctgacagg tggtggcaat
      721 gccccaacca gtgggggttg cgtcagcaaa cacagtgcac accacgccac gttgcctgac
      781 aacgggccac aactcctcat aaagagacag caaccaggat ttatacaagg aggagaaaat
      841 gaaagccata cgggaagcaa tagcatgata caaaggcatt aaagcagcgt atccacatag
      901 cgtaaaagga gcaacatagt taagaatacc agtcaatctt tcacaaattt tgtaatccag
      961 aggttgatta tcgataagct tttaggaaat ctccagagta gacagccagc gcgcgatctc
     1021 gctgccgatc aggtccgggt tgtcttcttg cagcagattc agacccgggc cgatgtccac
     1081 agccttgcag ttaggcaggc ttttggccag gcgagcggct tcggccggtg ggatcagaac
     1141 gcctggggtg ccccagaaca gcagcttcgg gacaggggac tggtgcagcc agtccatgta
     1201 ttcttcgacc agcgcgacga tgttcgctgg ctcaccggcg attggcagct cgtttgggaa
     1261 gcgccacagt ggctcgcggt caacaggatt caggaacggc tcgcggtaat ggtccatctc
     1321 gacttcagtc agcgggcgga cgacacccat cggcagcgta ccctcgataa aaacgttctg
     1381 atcgatgatc agcttgcggc cgacgtcggt ggtgcggaag gcctggaagg tctcgcgggc
     1441 aaattctggc cattcgtccc aggtcgggat agggcggatg aactccataa atgcaatacc
     1501 tttgacgcgc tctggattgc gcttggccca gtggaaaccc agagcggagc cccagtcgtg
     1561 aatgaccagg acgacctctt ccagacccag ggcttcgatg aaggcatcca tgaagcggac
     1621 gtggtcgtcg aagaaataac ccaggtctgg tttgtcggat ttgcccatac cgatcaggtc
     1681 tggagcaatg cagcgatggg tcggtgcaac atgcgggatg atgttgcgcc acacgtagga
     1741 ggaggtcggg ttaccgtgca ggaacagcac aggggtgcca tcgcgcggac caacatcgac
     1801 gtagtgcatg cgctcgccca ggacttccac ataatggggg tcgaatggaa agccagtacc
     1861 gatttctgcg ctgccgccgc cagatccgcc gccgctgcct ccgccgccgg taccggcctt
     1921 ggtggcggcc tggctcaggc ctctcagggg cacggcggcc tcagggccca ggcatctgaa
     1981 ggtcatgctc tcctgcaggt acaggtgcac gctctgggca tcgtggtcgt ggtatccgat
     2041 gctgaaatct ctgcccactg tcagctcaaa gtcgccgcct ctctggctga tcagcatggc
     2101 gccatcaact ccaggggccc agatcagagg gccctcgaac agtctccgca cgtgttgaag
     2161 cacagggtag ccgccagggg ttgtggtttc catcagctgc tggtacacgg tccttcccag
     2221 gaccagggcg taggggccgt ccacgcctct gtcccgcagc acagcgaggg cgcgcaccag
     2281 cacgccgggg aagtcggcga ggtcggcagg caggtccagg gcgtgttcag cgctggcttc
     2341 tccgatgcct gtgatgccgc ctgcggcgaa gccatgaaag acggcccggt cctcggcgat
     2401 ggcgatggct ctagcggcct cgataacagg gtccagatcg gcatcagagg ctcctctcgc
     2461 gatagcttcc agttcagctc ttttcagggt gaatggcaca cacagctcga tcagaggctg
     2521 cacagcccgc ttccgcactg tgaccacagc ggcgctgccg gcggccttag gttcctcagc
     2581 cagggcctcg gttctgccca gggacacaga ggaggcgtcc cagcccaggg ggcccttgaa
     2641 gtccaccact tttctggcgg ccagggtcac ggtcagggtg ccccgggcct ctgtctcgat
     2701 ttcggcccag gccttggcgc taattggagc caggtctctc atcaggtcgt tcatggtggc
     2761 ggatcctcga gatcccgggc ccgcggtatc aaatcaattc tttgccaaaa tgatgagaca
     2821 gcacaataac cagcacgttg cccaggagct gtaggaaaaa gaagaaggca tgaacatggt
     2881 tagcagaggc tctagagccg ccggtcacac gccagaagcc gaaccccgcc ctgccccgtc
     2941 ccccccgaag gcagccgtcc ccccgcggac agccccgagg ctggagaggg agaaggggac
     3001 ggcggcgcgg cgacgcacga aggccctccc cgcccatttc cttcctgccg gcgccgcacc
     3061 gcttcgcccc gcgcccgcta gagggggtgc ggcggcgcct cccagatttc ggctccgcac
     3121 agatttggga caaaggaagt ccctgcgccc tctcgcacga ttaccataaa aggcaatggc
     3181 tgcggctcgc cgcgcctcga cagccgccgg cgctccgggg accgccgcgc ccctcccccg
     3241 agccctcccc ggcccgaggc ggccccgccc cgcccggcac ccccacctgc cgccaccccc
     3301 cgcccggcac ggcgagcccc gcgccacgcc ccgtacggag ccccgcaccc gaagccgggc
     3361 cgtgctcagc aactcgggga ggggggtgca gggggggttg cagcccgacc gacgcgccca
     3421 caccccctgc tcaccccccc acgcacacac cccgcacgca gcctttgttc ccctcgcagc
     3481 cccccccgca ccgcggggca ccgcccccgg ccgcgctccc ctcgcgcaca ctgcggagcg
     3541 cacaaagccc cgcgccgcgc ccgcagcgct cacagccgcc gggcagcgcg gagccgcacg
     3601 cggcgctccc cacgcacaca cacacgcacg caccccccga gccgctcccc ccgcacaaag
     3661 ggccctcccg gagcccctca aggctttcac gcagccacag aaaagaaaca agccgtcatt
     3721 aaaccaagcg ctaattacag cccggaggag aagggccgtc ccgcccgctc acctgtggga
     3781 gtaacgcggt cagtcagagc cggggcgggc ggcgcgaggc ggcggcggag cggggcacgg
     3841 ggcgaaggca gcgcgcagcg actcccgccc gccgcgcgct tcgcttttta tagggccgcc
     3901 gccgccgccg cctcgccata aaaggaaact ttcggagcgc gccgctctga ttggctgccg
     3961 ccgcacctct ccgcctcgcc ccgccccgcc cctcgccccg ccccgccccg cctggcgcgc
     4021 gccccccccc ccccgccccc atcgctgcac aaaataatta aaaaataaat aaatacaaaa
     4081 ttgggggtgg ggaggggggg gagatgggga gagtgaagca gaacgtgggg ctcacctcga
     4141 ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg
     4201 tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg
     4261 tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca
     4321 cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg
     4381 acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg
     4441 taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat
     4501 aactagttaa ttaaccaaac tggacgcgtg cggccgcagg aacccctagt gatggagttg
     4561 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga
     4621 cgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggaca tgtgagcaaa
     4681 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     4741 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     4801 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     4861 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     4921 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     4981 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     5041 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     5101 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     5161 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     5221 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     5281 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     5341 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     5401 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     5461 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     5521 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     5581 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     5641 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     5701 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     5761 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     5821 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     5881 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     5941 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     6001 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     6061 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     6121 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     6181 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     6241 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     6301 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     6361 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     6421 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     6481 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     6541 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     6601 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     6661 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
     6721 gagagtgcac cataaaattg taaacgttaa tattttgtta aaattcgcgt taaatttttg
     6781 ttaaatcagc tcatttttta accaatagac cgaaatcggc aaaatccctt ataaatcaaa
     6841 agaatagccc gagatagagt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     6901 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     6961 tgaaccatca cccaaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     7021 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     7081 ggaagggaag aaagcgaaag gagcgggcgc taaggcgctg gcaagtgtag cggtcacgct
     7141 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt actatggttg
     7201 ctttgacgta tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     7261 cgcccctgca ggcagctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg
     7321 cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg
     7381 gccaactcca tcactagggg ttcctgcggc cgctcggtcc gcacgtggtt acctacaaaa
     7441 tcagaaggac agggaaggga gcagtggttc acgcctgtaa tcccagcaat ttgggaggcc
     7501 aaggtgggta gatcacctga gattaggagt tggagaccag cctggccaat atggtgaaac
     7561 cccgtctcta cc
//