LOCUS       03-AAV-EGFP-APOB        6213 bp DNA     circular SYN 18-SEP-2023
DEFINITION  .
ACCESSION   urn.local...49-g17gq3r
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6213)
  AUTHORS   Rui Lin
  TITLE     Direct Submission
  JOURNAL   Exported Sep 19, 2023 from SnapGene 7.0.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6213
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(3..11)
                     /standard_name="glycine-serine-glycine linker"
                     /label=glycine-serine-glycine linker
                     /note="color: #a6acb3; direction: LEFT"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /note="color: #ffff00"
     CDS             complement(941..1801)
                     /gene="bla"
                     /standard_name="AmpR"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin,and 
                     related antibiotics"
                     /note="color: #993366; direction: LEFT"
     promoter        complement(1802..1906)
                     /gene="bla"
                     /standard_name="AmpR promoter"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      complement(1932..2387)
                     /direction=LEFT
                     /note="color: #ffff00"
     promoter        2901..3178
                     /standard_name="chicken beta-actin promoter"
                     /label=chicken beta-actin promoter
                     /note="color: #ffffff"
     CDS             3606..4322
                     /standard_name="EGFP"
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="color: #993366; direction: RIGHT"
     misc_feature    4259..4280
                     /standard_name="EGFP-C_F"
                     /label=EGFP-C_seq_F
                     /note="Geneious type: primer"
                     /note="EGFP, forward primer"
                     /note="sequence: CATGGTCCTGCTGGAGTTCGTG"
                     /note="color: #a6acb3"
     misc_feature    4303..4339
                     /standard_name="EGFP_SV40_P2A_F"
                     /label=EGFP_P2A_clone_F
                     /note="Geneious type: primer"
                     /note="sequence: gcatggacgagctgtacaaggccactaacttctccct"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4323..4379
                     /standard_name="SVNLS_P2A_a_F"
                     /label=P2A_clone_F
                     /note="Geneious type: primer"
                     /note="sequence: 
                     gccactaacttctccctgttgaaacaagcaggggatgtcgaagagaatcccgggcca"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4323..4379
                     /standard_name="P2A"
                     /label=P2A
                     /note="color: #a6acb3"
     misc_feature    4362..4399
                     /standard_name="P2A_APOBEC1_YTH"
                     /label=P2A_APOBEC1_clone_F
                     /note="Geneious type: primer"
                     /note="sequence: gaagagaatcccgggccaatgagctcagagactggccc"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4380..5072
                     /standard_name="APOBEC1"
                     /label=APOBEC1
                     /note="color: #a6acb3"
     misc_feature    complement(5047..5099)
                     /standard_name="V4_HA_XhoI_APOBEC1_R"
                     /label=HA_APOBEC1_clone_R
                     /note="Geneious type: primer"
                     /note="sequence: 
                     ggcgtagtcgggcacgtcgtaggggtactcgagtttcaacccggtggcccaga"
                     /note="color: #a6acb3; direction: LEFT"
     CDS             5073..5099
                     /standard_name="HA"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="color: #993366; direction: RIGHT"
     misc_feature    complement(5080..5128)
                     /standard_name="WPRE-HindIII-SD-HA-APOYTH-R"
                     /label=WPRE-HindIII-SD-HA-clone-R
                     /note="Geneious type: primer"
                     /note="sequence: 
                     tccagaggttgattatcgataagcttttaggcgtagtcgggcacgtcgt"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    5115..5703
                     /standard_name="WPRE"
                     /label=WPRE
                     /note="woodchuck hepatitis virus 
                     posttranscriptionalregulatory element"
                     /note="color: #a6acb3"
     CDS             complement(5586..5597)
                     /standard_name="Factor Xa site"
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #993366; direction: LEFT"
     polyA_signal    5746..5867
                     /label=SV40 polyadenylation signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
     2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     2461 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg
     2521 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
     2581 gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag
     2641 gaagatcgta ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     2701 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     2761 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     2821 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     2881 agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc
     2941 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
     3001 gggggcgggg gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg
     3061 cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc
     3121 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg
     3181 agtcgctgcg acgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
     3241 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
     3301 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
     3361 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ctgtccgcgg
     3421 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     3481 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     3541 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattgga tccggtaccg
     3601 ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     3661 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     3721 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     3781 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     3841 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     3901 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     3961 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     4021 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     4081 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     4141 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     4201 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     4261 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     4321 aggccactaa cttctccctg ttgaaacaag caggggatgt cgaagagaat cccgggccaa
     4381 tgagctcaga gactggccca gtggctgtgg accccacatt gagacggcgg atcgagcccc
     4441 atgagtttga ggtattcttc gatccgagag agctccgcaa ggagacctgc ctgctttacg
     4501 aaattaattg ggggggccgg cactccattt ggcgacatac atcacagaac actaacaagc
     4561 acgtcgaagt caacttcatc gagaagttca cgacagaaag atatttctgt ccgaacacaa
     4621 ggtgcagcat tacctggttt ctcagctgga gcccatgcgg cgaatgtagt agggccatca
     4681 ctgaattcct gtcaaggtat ccccacgtca ctctgtttat ttacatcgca aggctgtacc
     4741 accacgctga cccccgcaat cgacaaggcc tgcgggattt gatctcttca ggtgtgacta
     4801 tccaaattat gactgagcag gagtcaggat actgctggag aaactttgtg aattatagcc
     4861 cgagtaatga agcccactgg cctaggtatc cccatctgtg ggtacgactg tacgttcttg
     4921 aactgtactg catcatactg ggcctgcctc cttgtctcaa cattctgaga aggaagcagc
     4981 cacagctgac attctttacc atcgctcttc agtcttgtca ttaccagcga ctgcccccac
     5041 acattctctg ggccaccggg ttgaaactcg agtaccccta cgacgtgccc gactacgcct
     5101 aaaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     5161 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     5221 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     5281 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     5341 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     5401 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     5461 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     5521 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     5581 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     5641 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     5701 cgcatcgata ccgtcgaccc gggcggccgc ttcgagcaga catgataaga tacattgatg
     5761 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     5821 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     5881 gcattcattt tatgtttcag gttcaggggg agatgtggga ggttttttaa agcaagtaaa
     5941 acctctacaa atgtggtaaa atcgataagg atcttcctag agcatggcta cgtagataag
     6001 tagcatggcg ggttaatcat taactacaag gaacccctag tgatggagtt ggccactccc
     6061 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc
     6121 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcattaatga atcggccaac
     6181 gcgcggggag aggcggtttg cgtattgggc gct
//
