LOCUS       pBWB507_ME_mRFP_U2_barc 3667 bp ds-DNA     circular     19-SEP-2023
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name: pAD200.gb
            ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    1..627
                     /label="tetR"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     primer          5..27
                     /label="BWB1418"
                     /note="sequence: gacccactttcacatttaagttg"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          533..554
                     /label="BWB1417"
                     /note="sequence: gcgagtttacgggttgttaaac"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    628..700
                     /label="tet promoter region"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    649..667
                     /label="Q2 operator (TetR )"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_fwdcolor="#800080"
                     /note="ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    649..667
                     /label="Q1 operator (tetR)"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_fwdcolor="#800080"
                     /note="ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     primer          complement(686..724)
                     /label="BWB1416"
                     /note="sequence: attgtcgacatatggtcTGAATTCTTTTCTCTATCACTG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(686..732)
                     /label="BWB1439"
                     /note="sequence: CTGTCTCTTATACACATCTatggtctgaattcttttctctatcactg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(688..713)
                     /label="BWB1422"
                     /note="sequence: atggtctgaattcttttctctatcac"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(690..716)
                     /label="BWB1424"
                     /note="sequence: catatggtctgaattcttttctctatc"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    701..713
                     /label="Could remove potentially"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    708..713
                     /label="Eam1105I site 1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    714..732
                     /label="Front"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          714..760
                     /label="BWB1440"
                     /note="sequence: AGATGTGTATAAGAGACAGtttaagaaggagatatacatatggcgag"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    733..752
                     /label="RBS(JBEI biobricks)"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_fwdcolor="#800000"
                     /note="ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    753..1430
                     /label="mRFP"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
                     /note="ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     CDS             753..1430
                     /label="Translation 753-1430"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMRLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHSTGA*"
     primer          complement(1411..1449)
                     /label="BWB1441"
                     /note="sequence: AGATGTGTATAAGAGACAGttaagcaccggtggagtgac"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1431..1449
                     /label="Back"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          1431..1509
                     /label="BWB1438"
                     /note="sequence: CTGTCTCTTATACACATCTGTCGACCTGCAGCGTACGNNNNNNNNNNNNNNNNNNNNAGAGACCTCGTGGACATCactg"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    complement(1450..1467)
                     /label="U2"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: cyan ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     primer          1450..1509
                     /label="BWB1431"
                     /note="sequence: GTCGACCTGCAGCGTACGNNNNNNNNNNNNNNNNNNNNAGAGACCTCGTGGACATCactg"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          1450..1525
                     /label="BWB1429"
                     /note="sequence: GTCGACCTGCAGCGTACGNNNNNNNNNNNNNNNNNNNNAGAGACCTCGTGGACATCactgtcTAGGGATAACAGGG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1468..1487
                     /label="Barcode"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          1468..1509
                     /label="BWB1431"
                     /note="sequence: NNNNNNNNNNNNNNNNNNNNAGAGACCTCGTGGACATCactg"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(1488..1505)
                     /label="U1"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: cyan ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     primer          1488..1525
                     /label="BWB1430"
                     /note="sequence: AGAGACCTCGTGGACATCactgtcTAGGGATAACAGGG"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          1495..1530
                     /label="BWB1415"
                     /note="sequence: cgtcctgacgcactgtcTAGGGATAACAGGGTAATG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          1505..1525
                     /label="BWB1423"
                     /note="sequence: cactgtcTAGGGATAACAGGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1506..1511
                     /label="Eam1105i site 2"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          1506..1525
                     /label="BWB1421"
                     /note="sequence: actgtcTAGGGATAACAGGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1512..1529
                     /label="I-SceI site"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: cyan ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    1530..1553
                     /label="oAD232_FORWARD"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="ApEinfo_revcolor: #ffff00 ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    1554..1682
                     /label="dbI terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    1687..2398
                     /label="p15A"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_fwdcolor="#808080"
                     /note="ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     primer          complement(1740..1759)
                     /label="BWB1419"
                     /note="sequence: gtaagccatttccgctcgcc"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    2466..2691
                     /label="oriT"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /note="ApEinfo_revcolor: #ff00ff ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    2692..2709
                     /label="oriT"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /note="locus_tag: oriT(1) ApEinfo_revcolor: #ff00ff ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    2755..2860
                     /label="To (terminator ?)"
                     /ApEinfo_revcolor="#808000"
                     /ApEinfo_fwdcolor="#808000"
                     /note="ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    2876..3535
                     /label="cmR2"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     primer          complement(2981..3001)
                     /label="BWB130"
                     /note="sequence: CAAGGCGACAAGGTGCTGATG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          3127..3146
                     /label="BWB149"
                     /note="sequence: CTTTAGGGAAATAGGCCAGG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(3127..3146)
                     /label="BWB148"
                     /note="sequence: CCTGGCCTATTTCCCTAAAG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          3483..3505
                     /label="BWB129"
                     /note="sequence: CGATGCCATTGGGATATATCAAC"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(3483..3505)
                     /label="BWB128"
                     /note="sequence: GTTGATATATCCCAATGGCATCG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
ORIGIN
        1 ttaagaccca ctttcacatt taagttgttt ttctaatccg catatgatca attcaaggcc
       61 gaataagaag gctggctctg caccttggtg atcaaataat tcgatagctt gtcgtaataa
      121 tggcggcata ctatcagtag taggtgtttc cctttcttct ttagcgactt gatgctcttg
      181 atcttccaat acgcaaccta aagtaaaatg ccccacagcg ctgagtgcat ataatgcatt
      241 ctctagtgaa aaaccttgtt ggcataaaaa ggctaattga ttttcgagag tttcatactg
      301 tttttctgta ggccgtgtac ctaaatgtac ttttgctcca tcgcgatgac ttagtaaagc
      361 acatctaaaa cttttagcgt tattacgtaa aaaatcttgc cagctttccc cttctaaagg
      421 gcaaaagtga gtatggtgcc tatctaacat ctcaatggct aaggcgtcga gcaaagcccg
      481 cttatttttt acatgccaat acaatgtagg ctgctctaca cctagcttct gggcgagttt
      541 acgggttgtt aaaccttcga ttccgacctc attaagcagc tctaatgcgc tgttaatcac
      601 tttactttta tctaatctag acatcattaa ttcctaattt ttgttgacac tctatcgttg
      661 atagagttat tttaccactc cctatcagtg atagagaaaa gaattcagac catAGATGTG
      721 TATAAGAGAC AGtttaagaa ggagatatac atatggcgag tagcgaagac gttatcaaag
      781 agttcatgcg tttcaaagtt cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg
      841 aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg aaagttacca
      901 aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc gcagttccag tacggttcca
      961 aagcttacgt taaacacccg gctgacatcc cggactacct gaaactgtcc ttcccggaag
     1021 gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc gttacccagg
     1081 actcctccct gcaagacggt gagttcatct acaaagttaa actgcgtggt accaacttcc
     1141 cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta
     1201 tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg
     1261 gtggtcacta cgacgctgaa gttaaaacca cctacatggc taaaaaaccg gttcagctgc
     1321 cgggtgctta caaaaccgac atcaaactgg acatcacctc ccacaacgaa gactacacca
     1381 tcgttgaaca gtacgaacgt gctgaaggtc gtcactcAac cggtgcttaa CTGTCTCTTA
     1441 TACACATCTG TCGACCTGCA GCGTACGTAA CATCGTCCGA TACTGTAAGA GACCTCGTGG
     1501 ACATCactgt cTAGGGATAA CAGGGTAATG GATCCAAACT CGAGTAAGGA TCTccaggca
     1561 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     1621 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     1681 taccgggata tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     1741 gcgagcggaa atggcttacg aacggggcgg agatttcctg gaagatgcca ggaagatact
     1801 taacagggaa gtgagagggc cgcggcaaag ccgtttttcc ataggctccg cccccctgac
     1861 aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga
     1921 taccaggcgt ttccccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt
     1981 accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc
     2041 cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg
     2101 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc
     2161 actggcagca gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt
     2221 aaggctaaac tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt
     2281 tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt
     2341 tttcagagca agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc
     2401 agataaaata tttctagatt tcagtgcaat ttatctcttc aaatgtagca cctgTTCTTG
     2461 CTTAtCGGCC AGCCTCGCAG AGCAGGATTC CCGTTGAGCA CCGCCAGGTG CGAATAAGGG
     2521 ACAGTGAAGA AGGAACACCC GCTCGCGGGT GGGCCTACTT CACCTATCCT GCCCGGCTGA
     2581 CGCCGTTGGA TACACCAAGG AAAGTCTACA CGAACCCTTT GGCAAAATCC TGTATATCGT
     2641 GCGAAAAAGG ATGGATATAC CGAAAAAATC GCTATAATga ccccgaagca gGGTTATGCA
     2701 GCGGAAAAGa atgtcatgat aagtcagccc catacgatat aagttgttac tagtgcttgg
     2761 attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
     2821 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg
     2881 ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
     2941 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
     3001 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
     3061 ttaaatcaaa actggtgaaa ctcacccagg gattggctGA aACGaaaaac atattctcaa
     3121 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
     3181 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag
     3241 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
     3301 ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
     3361 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
     3421 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
     3481 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
     3541 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
     3601 cattatggtg aaagttggaa cctcttacgt gccgatcaag GTCTCatttt cgccagatat
     3661 cgacgtc
//