LOCUS       Exported               13071 bp ds-DNA     circular SYN 07-OCT-2022
DEFINITION  Gateway cloning compatible binary vector for C-terminal fusion with 
            sGFP (CaMV35S promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    pGW-TurboID-inCter
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13071)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 13071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Sep 19, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13071
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1179..1303
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1287..1304)
                     /label=GW-TurboID-GFP-Fw1
     promoter        1340..1370
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1424..2083
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1490..1509)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2425..2730
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2609..2628
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     2765..2787
                     /label=GW-TurboID-GFP-Fw1
     protein_bind    complement(2771..2895)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2892..2934)
                     /label=GW-noATG-TurboID-Cter-Rv1
     CDS             2915..2941
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2945..2971
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2975..3001
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3008..3964
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     primer_bind     complement(3947..3982)
                     /label=GW-TurboID-Cter-Rv2
     terminator      3992..4244
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4278..4294)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4278..4294)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4291..4313)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4302..4318
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4326..4356)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4371..4392
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      4428..4680
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4831..5856)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5908..6087)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6593..6617
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7138..7929
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8175..8763
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8664..8683
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8917..8934
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8949..9089
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9015..9034)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9175..9197
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9433..9627
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9693..10766)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11195..11824)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg
     1201 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     1261 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgaggggc
     1321 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     1381 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     1441 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1501 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1561 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1621 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     1681 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1741 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1801 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     1861 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1921 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     1981 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     2041 tgaattacaa cagtactgcg atgagtggca gggcggggcg taatctagag gatccggctt
     2101 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     2161 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca
     2221 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     2281 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     2341 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     2401 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     2461 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     2521 gtgatccccc tggccagtgc acgtctgctg tcagataaag tcccccgtga actttacccg
     2581 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     2641 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     2701 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     2761 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     2821 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     2881 cttgtacaaa gtggttcgat ctagaggatc catgtacccg tatgatgttc cggattacgc
     2941 tggctatccc tacgacgtgc ccgactatgc cgggtacccc tatgacgtcc cagactacgc
     3001 agctagcaaa gacaatactg tgcctctgaa gctgatcgct ctcctggcta atggcgagtt
     3061 ccatagtggc gaacagctgg gagaaaccct gggcatgtcc agggccgcta tcaacaagca
     3121 cattcagact ctgcgcgact ggggcgtgga cgtgttcacc gtgcccggaa agggctactc
     3181 tctgcccgag cctatcccgc tgctgaacgc taaacagatt ctgggacagc tggacggcgg
     3241 gagcgtggca gtcctgcctg tggtagactc caccaatcag tacctgctgg atcgaatcgg
     3301 cgagctgaag agtggggatg cttgcattgc agaatatcag caggcaggga gaggaagcag
     3361 agggaggaaa tggttctctc cttttggagc taacctgtac ctgagtatgt tttggcgcct
     3421 gaagcgggga ccagcagcaa tcggcctggg cccggtcatc ggaattgtca tggcagaagc
     3481 gctgcgaaag ctgggagcag acaaggtgcg agtcaaatgg cccaatgacc tgtatctgca
     3541 ggatagaaag ctggcaggca tcctggtgga gctggccgga ataacaggcg atgctgcaca
     3601 gatcgtcatt ggcgccggga ttaacgtggc tatgaggcgc gtggaggaaa gcgtggtcaa
     3661 tcagggctgg atcacactgc aggaagcagg gattaacctg gacaggaata ctctggccgc
     3721 tacgctgatc cgagagctgc gggcagccct ggaactgttc gagcaggaag gcctggctcc
     3781 atatctgcca cggtgggaga agctggataa cttcatcaat agacccgtga agctgatcat
     3841 tggggacaaa gagattttcg ggattagccg ggggattgat aaacagggag ccctgctgct
     3901 ggaacaggac ggagttatca aaccctggat gggcggagaa atcagtctgc ggtctgccga
     3961 aaagtagagc ggcccgagct cgaatttccc cgatcgttca aacatttggc aataaagttt
     4021 cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta
     4081 cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat
     4141 gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa
     4201 ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat tggttccgga
     4261 accaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     4321 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     4381 taactcacat taggctgaat taggcgcgcc tatttctatt aattcccgat ctagtaacat
     4441 agatgacacc gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc
     4501 gtattaaatg tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat
     4561 gcattacatg ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat
     4621 cgcaagaccg gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc
     4681 ggggaaattc gggggatcga tcccgcgaac tgtggacgag aactgtgcca ccaagcgtaa
     4741 ggccgttctc tcgcatttgc cttgctaggc tcgcgcgagt tgctggctga ggcgttctcg
     4801 aaatcagctc ttgttcggtc ggcatctact ctattccttt gccctcggac gagtgctggg
     4861 gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct
     4921 tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca
     4981 tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg
     5041 gtcaagacca atgcggagca tatacgcccg gagccgcggc gatcctgcaa gctccggatg
     5101 cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa
     5161 gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt caatgaccgc
     5221 tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt gcacgaggtg
     5281 ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct gcgcgacgga
     5341 cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat cagcaatcgc
     5401 gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga atgggccgaa
     5461 cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atggcctccg cgaccggctg
     5521 cagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg
     5581 ggagatgcaa taggtcaggc tctcgctgaa tgccccaatg tcaagcactt ccggaatcgg
     5641 gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac catcggcgca
     5701 gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag cacgagattc
     5761 ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga tcagaaactt
     5821 ctcgacagac gtcgcggtga gttcaggctt tttcatatct tattgccccc cgggatcaga
     5881 tctggattga gagtgaatat gagactctaa ttggataccg aggggaattt atggaacgtc
     5941 agtggagcat ttttgacaag aaatatttgc tagctgatag tgaccttagg cgacttttga
     6001 acgcgcaata atggtttctg acgtatgtgc ttagctcatt aaactccaga aacccgcggc
     6061 tgagtggctc cttcaacgtt gcggttctgt cagttccaaa cgtaaaacgg cttgtcccgc
     6121 gtcatcggcg ggggtcataa cgtgactccc ttaattctcc gctcatgatc ttgatcccct
     6181 gcgccatcag atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac
     6241 cttaccagag ggcgccccag ctggcaattc cggttcgctt gctgtccata aaaccgccca
     6301 gtctagctat cgccatgtaa gcccactgca agctacctgc tttctctttg cgcttgcgtt
     6361 ttcccttgtc cagatagccc agtagctgac attcatccgg ggtcagcacc gtttctgcgg
     6421 actggctttc tacgtgttcc gcttccttta gcagcccttg cgccctgagt gcttgcggca
     6481 gcgtgaagct gatccgtcga gattaataga aataaattca gcctaattcg gcgttaattc
     6541 agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat ttgtttacac
     6601 cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc ggcacaaaat
     6661 caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa
     6721 ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact aagctgccgg
     6781 gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat gacagagcgt
     6841 tgctgcctgt gatcaattcg ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
     6901 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
     6961 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
     7021 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
     7081 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg
     7141 ggggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
     7201 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
     7261 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
     7321 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
     7381 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
     7441 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
     7501 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
     7561 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
     7621 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
     7681 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
     7741 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     7801 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     7861 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     7921 ggcaaataat gtctagctag aaattcgttc aagccgacgc cgcttcgcgg cgcggcttaa
     7981 ctcaagcgtt agatgcacta agcacataat tgctcacagc caaactatca ggtcaagtct
     8041 gcttttatta tttttaagcg tgcataataa gccctacaca aattgggaga tatatcatgc
     8101 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     8161 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     8221 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     8281 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     8341 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     8401 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     8461 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     8521 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     8581 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     8641 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     8701 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     8761 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     8821 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     8881 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     8941 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     9001 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc
     9061 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc
     9121 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     9181 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga
     9241 tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct
     9301 ggccgtaggc cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg
     9361 gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc
     9421 tggccagaca gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag
     9481 ttttaggcgg aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg
     9541 ttcccaatgt acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt
     9601 gggttcccaa tgtacgtgct atccacagga aagagacctt ttcgaccttt ttcccctgct
     9661 agggcaattt gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg
     9721 atcaggttgc ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa
     9781 tcgtactccg gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg
     9841 aactctccgg cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc
     9901 ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa
     9961 aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac
    10021 atccaatcag ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc
    10081 ttgtagcggc taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc
    10141 ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg
    10201 tcgtctttct gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt
    10261 ggacggaaca cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg
    10321 gttagatggg aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc
    10381 ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct
    10441 cgtcggtcac gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg
    10501 gtgcccacgt catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc
    10561 ttcctaatcg acggcgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca
    10621 gcggccgctt gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg
    10681 gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc
    10741 gggccggatg gtttgcgacc gctcacgccg attcctcggg cttgggggtt ccagtgccat
    10801 tgcagggccg gcagacaacc cagccgctta cgcctggcca accgcccgtt cctccacaca
    10861 tggggcattc cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg cctcctttag
    10921 ccgctaaaat tcatctactc atttattcat ttgctcattt actctggtag ctgcgcgatg
    10981 tattcagata gcagctcggt aatggtcttg ccttggcgta ccgcgtacat cttcagcttg
    11041 gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca tggctggcgt gtctgccagg
    11101 ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac ggccggcact tagcgtgttt
    11161 gtgcttttgc tcattttctc tttacctcat taactcaaat gagttttgat ttaatttcag
    11221 cggccagcgc ctggacctcg cgggcagcgt cgccctcggg ttctgattca agaacggttg
    11281 tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt gatacgggac tcaagaatgg
    11341 gcagctcgta cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg
    11401 cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa
    11461 ccagctccac caggtcggcg gtggcccata tgtcgtaagg gcttggctgc accggaatca
    11521 gcacgaagtc ggctgccttg atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt
    11581 cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt
    11641 cgatgccgac aacggttagc ggttgatctt cccgcacggc cgcccaatcg cgggcactgc
    11701 cctggggatc ggaatcgact aacagaacat cggccccggc gagttgcagg gcgcgggcta
    11761 gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca gcgataacct
    11821 tcatgcgttc cccttgcgta tttgtttatt tactcatcgc atcatatacg cagcgaccgc
    11881 atgacgcaag ctgttttact caaatacaca tcaccttttt agacggcggc gctcggtttc
    11941 ttcagcggcc aagctggccg gccaggccgc cagcttggca tcagacaaac cggccaggat
    12001 ttcatgcagc cgcacggttg agacgtgcgc gggcggctcg aacacgtacc cggccgcgat
    12061 catctccgcc tcgatctctt cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg
    12121 tttcatgctt gttcctcttg gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc
    12181 aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg
    12241 cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc
    12301 acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg
    12361 gtagggcggg ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg
    12421 cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt caacaccatg
    12481 cggccggccg gcgtggtggt gtcggcccac ggctctgcca ggctacgcag gcccgcgccg
    12541 gcctcctgga tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc caggcggtct
    12601 agcctggtca ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct ggccagctcc
    12661 gggcggtcgc gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg
    12721 tgcagttcgg cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc
    12781 agcaggccag cggcggcgct cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta
    12841 ttctacttta tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg cagggcggca
    12901 gcctgtcgcg taacttagga cttgtgcgac atgtcgtttt cagaagacgg ctgcactgaa
    12961 cgtcagaagc cgactgcact atagcagcgg aggggttgga tcaaagtact ttgatcccga
    13021 ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc t
//
