LOCUS       Exported               13785 bp ds-DNA     circular SYN 06-APR-2022
DEFINITION  Gateway cloning compatible binary vector for C-terminal fusion with 
            sGFP (CaMV35S promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    pGW-TurboID-GFP-inCter
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13785)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 13785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Sep 19, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13785
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1179..1303
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1340..1370
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1424..2083
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1490..1509)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2425..2730
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2609..2628
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2771..2895)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2915..2941
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2945..2971
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2975..3001
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3008..3964
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     primer_bind     complement(3563..3579)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             3965..4681
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(4007..4028)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4268..4287)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4615..4636
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      4706..4958
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4992..5008)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4992..5008)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5005..5027)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5016..5032
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5040..5070)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5085..5106
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      5142..5394
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5545..6570)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(6622..6801)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    7307..7331
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7852..8643
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8889..9477
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9378..9397
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9631..9648
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9663..9803
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9729..9748)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9889..9911
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10147..10341
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10407..11480)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11909..12538)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg
     1201 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     1261 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgaggggc
     1321 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     1381 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     1441 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1501 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1561 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1621 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     1681 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1741 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1801 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     1861 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1921 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     1981 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     2041 tgaattacaa cagtactgcg atgagtggca gggcggggcg taatctagag gatccggctt
     2101 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     2161 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca
     2221 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     2281 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     2341 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     2401 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     2461 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     2521 gtgatccccc tggccagtgc acgtctgctg tcagataaag tcccccgtga actttacccg
     2581 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     2641 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     2701 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     2761 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     2821 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     2881 cttgtacaaa gtggttcgat ctagaggatc catgtacccg tatgatgttc cggattacgc
     2941 tggctatccc tacgacgtgc ccgactatgc cgggtacccc tatgacgtcc cagactacgc
     3001 agctagcaaa gacaatactg tgcctctgaa gctgatcgct ctcctggcta atggcgagtt
     3061 ccatagtggc gaacagctgg gagaaaccct gggcatgtcc agggccgcta tcaacaagca
     3121 cattcagact ctgcgcgact ggggcgtgga cgtgttcacc gtgcccggaa agggctactc
     3181 tctgcccgag cctatcccgc tgctgaacgc taaacagatt ctgggacagc tggacggcgg
     3241 gagcgtggca gtcctgcctg tggtagactc caccaatcag tacctgctgg atcgaatcgg
     3301 cgagctgaag agtggggatg cttgcattgc agaatatcag caggcaggga gaggaagcag
     3361 agggaggaaa tggttctctc cttttggagc taacctgtac ctgagtatgt tttggcgcct
     3421 gaagcgggga ccagcagcaa tcggcctggg cccggtcatc ggaattgtca tggcagaagc
     3481 gctgcgaaag ctgggagcag acaaggtgcg agtcaaatgg cccaatgacc tgtatctgca
     3541 ggatagaaag ctggcaggca tcctggtgga gctggccgga ataacaggcg atgctgcaca
     3601 gatcgtcatt ggcgccggga ttaacgtggc tatgaggcgc gtggaggaaa gcgtggtcaa
     3661 tcagggctgg atcacactgc aggaagcagg gattaacctg gacaggaata ctctggccgc
     3721 tacgctgatc cgagagctgc gggcagccct ggaactgttc gagcaggaag gcctggctcc
     3781 atatctgcca cggtgggaga agctggataa cttcatcaat agacccgtga agctgatcat
     3841 tggggacaaa gagattttcg ggattagccg ggggattgat aaacagggag ccctgctgct
     3901 ggaacaggac ggagttatca aaccctggat gggcggagaa atcagtctgc ggtctgccga
     3961 aaagatgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     4021 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     4081 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     4141 cctcgtgacc accttcacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     4201 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     4261 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     4321 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     4381 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     4441 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     4501 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     4561 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     4621 cctgctggag ttcgtgaccg ccgccgggat cactcacggc atggacgagc tgtacaagta
     4681 aagcggcccg agctcgaatt tccccgatcg ttcaaacatt tggcaataaa gtttcttaag
     4741 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa
     4801 gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag
     4861 agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga
     4921 taaattatcg cgcgcggtgt catctatgtt actagatcgg gaattggttc cggaaccaat
     4981 tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     5041 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     5101 acattaggct gaattaggcg cgcctatttc tattaattcc cgatctagta acatagatga
     5161 caccgcgcgc gataatttat cctagtttgc gcgctatatt ttgttttcta tcgcgtatta
     5221 aatgtataat tgcgggactc taatcataaa aacccatctc ataaataacg tcatgcatta
     5281 catgttaatt attacatgct taacgtaatt caacagaaat tatatgataa tcatcgcaag
     5341 accggcaaca ggattcaatc ttaagaaact ttattgccaa atgtttgaac gatcggggaa
     5401 attcggggga tcgatcccgc gaactgtgga cgagaactgt gccaccaagc gtaaggccgt
     5461 tctctcgcat ttgccttgct aggctcgcgc gagttgctgg ctgaggcgtt ctcgaaatca
     5521 gctcttgttc ggtcggcatc tactctattc ctttgccctc ggacgagtgc tggggcgtcg
     5581 gtttccacta tcggcgagta cttctacaca gccatcggtc cagacggccg cgcttctgcg
     5641 ggcgatttgt gtacgcccga cagtcccggc tccggatcgg acgattgcgt cgcatcgacc
     5701 ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag ctctgataga gttggtcaag
     5761 accaatgcgg agcatatacg cccggagccg cggcgatcct gcaagctccg gatgcctccg
     5821 ctcgaagtag cgcgtctgct gctccataca agccaaccac ggcctccaga agaagatgtt
     5881 ggcgacctcg tattgggaat ccccgaacat cgcctcgctc cagtcaatga ccgctgttat
     5941 gcggccattg tccgtcagga cattgttgga gccgaaatcc gcgtgcacga ggtgccggac
     6001 ttcggggcag tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga cggacgcact
     6061 gacggtgtcg tccatcacag tttgccagtg atacacatgg ggatcagcaa tcgcgcatat
     6121 gaaatcacgc catgtagtgt attgaccgat tccttgcggt ccgaatgggc cgaacccgct
     6181 cgtctggcta agatcggccg cagcgatcgc atccatggcc tccgcgaccg gctgcagaac
     6241 agcgggcagt tcggtttcag gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat
     6301 gcaataggtc aggctctcgc tgaatgcccc aatgtcaagc acttccggaa tcgggagcgc
     6361 ggccgatgca aagtgccgat aaacataacg atctttgtag aaaccatcgg cgcagctatt
     6421 tacccgcagg acatatccac gccctcctac atcgaagctg aaagcacgag attcttcgcc
     6481 ctccgagagc tgcatcaggt cggagacgct gtcgaacttt tcgatcagaa acttctcgac
     6541 agacgtcgcg gtgagttcag gctttttcat atcttattgc cccccgggat cagatctgga
     6601 ttgagagtga atatgagact ctaattggat accgagggga atttatggaa cgtcagtgga
     6661 gcatttttga caagaaatat ttgctagctg atagtgacct taggcgactt ttgaacgcgc
     6721 aataatggtt tctgacgtat gtgcttagct cattaaactc cagaaacccg cggctgagtg
     6781 gctccttcaa cgttgcggtt ctgtcagttc caaacgtaaa acggcttgtc ccgcgtcatc
     6841 ggcgggggtc ataacgtgac tcccttaatt ctccgctcat gatcttgatc ccctgcgcca
     6901 tcagatcctt ggcggcaaga aagccatcca gtttactttg cagggcttcc caaccttacc
     6961 agagggcgcc ccagctggca attccggttc gcttgctgtc cataaaaccg cccagtctag
     7021 ctatcgccat gtaagcccac tgcaagctac ctgctttctc tttgcgcttg cgttttccct
     7081 tgtccagata gcccagtagc tgacattcat ccggggtcag caccgtttct gcggactggc
     7141 tttctacgtg ttccgcttcc tttagcagcc cttgcgccct gagtgcttgc ggcagcgtga
     7201 agctgatccg tcgagattaa tagaaataaa ttcagcctaa ttcggcgtta attcagtaca
     7261 ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat
     7321 atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca aaatcaccac
     7381 tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc
     7441 agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg ccgggtttga
     7501 aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc
     7561 ctgtgatcaa ttcgggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa
     7621 tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt
     7681 aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg
     7741 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
     7801 agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgggggaa
     7861 gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat
     7921 ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag
     7981 ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg
     8041 cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc
     8101 cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct
     8161 aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag
     8221 ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt
     8281 gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt
     8341 gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag
     8401 cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg
     8461 ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc
     8521 atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca
     8581 gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa
     8641 taatgtctag ctagaaattc gttcaagccg acgccgcttc gcggcgcggc ttaactcaag
     8701 cgttagatgc actaagcaca taattgctca cagccaaact atcaggtcaa gtctgctttt
     8761 attattttta agcgtgcata ataagcccta cacaaattgg gagatatatc atgcatgacc
     8821 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     8881 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     8941 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     9001 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     9061 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     9121 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     9181 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     9241 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     9301 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     9361 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     9421 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     9481 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     9541 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     9601 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     9661 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
     9721 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc
     9781 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac
     9841 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     9901 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc ttgatgtggg
     9961 cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt gcctggccgt
    10021 aggccagcca tttttgagcg gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt
    10081 agggagcgca gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt gcgctggcca
    10141 gacagttatg cacaggccag gcgggtttta agagttttaa taagttttaa agagttttag
    10201 gcggaaaaat cgcctttttt ctcttttata tcagtcactt acatgtgtga ccggttccca
    10261 atgtacggct ttgggttccc aatgtacggg ttccggttcc caatgtacgg ctttgggttc
    10321 ccaatgtacg tgctatccac aggaaagaga ccttttcgac ctttttcccc tgctagggca
    10381 atttgcccta gcatctgctc cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg
    10441 ttgcggtagc gcatgactag gatcgggcca gcctgccccg cctcctcctt caaatcgtac
    10501 tccggcaggt catttgaccc gatcagcttg cgcacggtga aacagaactt cttgaactct
    10561 ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc atctggcttc tgccttgcct
    10621 gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa
    10681 tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg gtacatccaa
    10741 tcagctagct cgatctcgat gtactccggc cgcccggttt cgctctttac gatcttgtag
    10801 cggctaatca aggcttcacc ctcggatacc gtcaccaggc ggccgttctt ggccttcttc
    10861 gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac caggtcgtct
    10921 ttctgctttc cgccatcggc tcgccggcag aacttgagta cgtccgcaac gtgtggacgg
    10981 aacacgcggc cgggcttgtc tcccttccct tcccggtatc ggttcatgga ttcggttaga
    11041 tgggaaaccg ccatcagtac caggtcgtaa tcccacacac tggccatgcc ggccggccct
    11101 gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg
    11161 tcacgcttcg acagacggaa aacggccacg tccatgatgc tgcgactatc gcgggtgccc
    11221 acgtcataga gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg cggcttccta
    11281 atcgacggcg caccggctgc cggcggttgc cgggattctt tgcggattcg atcagcggcc
    11341 gcttgccacg attcaccggg gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc
    11401 gcggccttca acttctccac caggtcatca cccagcgccg cgccgatttg taccgggccg
    11461 gatggtttgc gaccgctcac gccgattcct cgggcttggg ggttccagtg ccattgcagg
    11521 gccggcagac aacccagccg cttacgcctg gccaaccgcc cgttcctcca cacatggggc
    11581 attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct ttagccgcta
    11641 aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc gatgtattca
    11701 gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag cttggtgtga
    11761 tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc caggctggcc
    11821 aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt
    11881 ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt tcagcggcca
    11941 gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg
    12001 cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga atgggcagct
    12061 cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg
    12121 acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc ttaaccagct
    12181 ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga atcagcacga
    12241 agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct ccgtcgatca
    12301 ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc
    12361 cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca ctgccctggg
    12421 gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg gctagatggg
    12481 ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata accttcatgc
    12541 gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga ccgcatgacg
    12601 caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg tttcttcagc
    12661 ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca ggatttcatg
    12721 cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg cgatcatctc
    12781 cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt gcggtttcat
    12841 gcttgttcct cttggcgttc attctcggcg gccgccaggg cgtcggcctc ggtcaatgcg
    12901 tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc gctgcgctca
    12961 agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg cagccgcctc tttcacggtg
    13021 cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt gagggtaggg
    13081 cgggggccaa acttcacgcc tcgggccttg gcggcctcgc gcccgctccg ggtgcggtcg
    13141 atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac catgcggccg
    13201 gccggcgtgg tggtgtcggc ccacggctct gccaggctac gcaggcccgc gccggcctcc
    13261 tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg gtctagcctg
    13321 gtcactgtca caacgtcgcc agggcgtagg tggtcaagca tcctggccag ctccgggcgg
    13381 tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg tgcagccggc cgcgtgcagt
    13441 tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata gcccagcagg
    13501 ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt tctagtcgca agtattctac
    13561 tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa agggcagggc ggcagcctgt
    13621 cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag acggctgcac tgaacgtcag
    13681 aagccgactg cactatagca gcggaggggt tggatcaaag tactttgatc ccgaggggaa
    13741 ccctgtggtt ggcatgcaca tacaaatgga cgaacggata aacct
//
