LOCUS       Exported               13780 bp ds-DNA     circular SYN 07-APR-2022
DEFINITION  Gateway cloning compatible binary vector for N-terminal fusion with 
            sGFP (CaMV35S promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    pGW-GFP-TurboID-inNter
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13780)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 13780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Sep 19, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13780
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1178..1891
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELY"
     primer_bind     complement(1484..1503)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1831..1852
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1898..1924
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1928..1954
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1958..1984
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1991..2947
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    2948..3072
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        3108..3138
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3192..3851
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3258..3277)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4193..4498
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4377..4396
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4539..4663)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      4701..4953
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4987..5003)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4987..5003)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5000..5022)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5011..5027
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5035..5065)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5080..5101
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      5137..5389
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5540..6565)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(6617..6796)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    7302..7326
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7847..8638
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8884..9472
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9373..9392
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9626..9643
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9658..9798
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9724..9743)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9884..9906
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10142..10336
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10402..11475)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11904..12533)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aggatccatg gtgagcaagg gcgaggagct
     1201 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtgaacg gccacaagtt
     1261 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1321 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcacctacgg
     1381 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1441 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1501 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1561 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1621 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     1681 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     1741 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     1801 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1861 cgggatcact cacggcatgg acgagctgta catcatgtac ccgtatgatg ttccggatta
     1921 cgctggctat ccctacgacg tgcccgacta tgccgggtac ccctatgacg tcccagacta
     1981 cgcagctagc aaagacaata ctgtgcctct gaagctgatc gctctcctgg ctaatggcga
     2041 gttccatagt ggcgaacagc tgggagaaac cctgggcatg tccagggccg ctatcaacaa
     2101 gcacattcag actctgcgcg actggggcgt ggacgtgttc accgtgcccg gaaagggcta
     2161 ctctctgccc gagcctatcc cgctgctgaa cgctaaacag attctgggac agctggacgg
     2221 cgggagcgtg gcagtcctgc ctgtggtaga ctccaccaat cagtacctgc tggatcgaat
     2281 cggcgagctg aagagtgggg atgcttgcat tgcagaatat cagcaggcag ggagaggaag
     2341 cagagggagg aaatggttct ctccttttgg agctaacctg tacctgagta tgttttggcg
     2401 cctgaagcgg ggaccagcag caatcggcct gggcccggtc atcggaattg tcatggcaga
     2461 agcgctgcga aagctgggag cagacaaggt gcgagtcaaa tggcccaatg acctgtatct
     2521 gcaggataga aagctggcag gcatcctggt ggagctggcc ggaataacag gcgatgctgc
     2581 acagatcgtc attggcgccg ggattaacgt ggctatgagg cgcgtggagg aaagcgtggt
     2641 caatcagggc tggatcacac tgcaggaagc agggattaac ctggacagga atactctggc
     2701 cgctacgctg atccgagagc tgcgggcagc cctggaactg ttcgagcagg aaggcctggc
     2761 tccatatctg ccacggtggg agaagctgga taacttcatc aatagacccg tgaagctgat
     2821 cattggggac aaagagattt tcgggattag ccgggggatt gataaacagg gagccctgct
     2881 gctggaacag gacggagtta tcaaaccctg gatgggcgga gaaatcagtc tgcggtctgc
     2941 cgaaaagaca agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa
     3001 tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat
     3061 ccagtcacta tggcggccct cgaggggccg cattaggcac cccaggcttt acactttatg
     3121 cttccggctc gtataatgtg tggattttga gttaggatcc ggcgagattt tcaggagcta
     3181 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
     3241 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
     3301 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
     3361 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa
     3421 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
     3481 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
     3541 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag
     3601 ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg
     3661 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt
     3721 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtctgtg
     3781 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg
     3841 gcggggcgta aacgcgtgga tccggcttac taaaagccag ataacagtat gcgtatttgc
     3901 gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa
     3961 gaggtgtgct atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct
     4021 caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc
     4081 cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc
     4141 cggtttattg aaatgaacgg ctcttttgct gacgagaaca gggactggtg aaatgcagtt
     4201 taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga
     4261 tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc
     4321 agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat
     4381 gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct
     4441 cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat
     4501 gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg atatgttgtg
     4561 ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta
     4621 tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggtgatgtac aagtaaagcg
     4681 gcccgagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga
     4741 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg
     4801 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
     4861 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat
     4921 tatcgcgcgc ggtgtcatct atgttactag atcgggaatt ggttccggaa ccaattcgta
     4981 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     5041 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     5101 aggctgaatt aggcgcgcct atttctatta attcccgatc tagtaacata gatgacaccg
     5161 cgcgcgataa tttatcctag tttgcgcgct atattttgtt ttctatcgcg tattaaatgt
     5221 ataattgcgg gactctaatc ataaaaaccc atctcataaa taacgtcatg cattacatgt
     5281 taattattac atgcttaacg taattcaaca gaaattatat gataatcatc gcaagaccgg
     5341 caacaggatt caatcttaag aaactttatt gccaaatgtt tgaacgatcg gggaaattcg
     5401 ggggatcgat cccgcgaact gtggacgaga actgtgccac caagcgtaag gccgttctct
     5461 cgcatttgcc ttgctaggct cgcgcgagtt gctggctgag gcgttctcga aatcagctct
     5521 tgttcggtcg gcatctactc tattcctttg ccctcggacg agtgctgggg cgtcggtttc
     5581 cactatcggc gagtacttct acacagccat cggtccagac ggccgcgctt ctgcgggcga
     5641 tttgtgtacg cccgacagtc ccggctccgg atcggacgat tgcgtcgcat cgaccctgcg
     5701 cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg tcaagaccaa
     5761 tgcggagcat atacgcccgg agccgcggcg atcctgcaag ctccggatgc ctccgctcga
     5821 agtagcgcgt ctgctgctcc atacaagcca accacggcct ccagaagaag atgttggcga
     5881 cctcgtattg ggaatccccg aacatcgcct cgctccagtc aatgaccgct gttatgcggc
     5941 cattgtccgt caggacattg ttggagccga aatccgcgtg cacgaggtgc cggacttcgg
     6001 ggcagtcctc ggcccaaagc atcagctcat cgagagcctg cgcgacggac gcactgacgg
     6061 tgtcgtccat cacagtttgc cagtgataca catggggatc agcaatcgcg catatgaaat
     6121 cacgccatgt agtgtattga ccgattcctt gcggtccgaa tgggccgaac ccgctcgtct
     6181 ggctaagatc ggccgcagcg atcgcatcca tggcctccgc gaccggctgc agaacagcgg
     6241 gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg tgcacggcgg gagatgcaat
     6301 aggtcaggct ctcgctgaat gccccaatgt caagcacttc cggaatcggg agcgcggccg
     6361 atgcaaagtg ccgataaaca taacgatctt tgtagaaacc atcggcgcag ctatttaccc
     6421 gcaggacata tccacgccct cctacatcga agctgaaagc acgagattct tcgccctccg
     6481 agagctgcat caggtcggag acgctgtcga acttttcgat cagaaacttc tcgacagacg
     6541 tcgcggtgag ttcaggcttt ttcatatctt attgcccccc gggatcagat ctggattgag
     6601 agtgaatatg agactctaat tggataccga ggggaattta tggaacgtca gtggagcatt
     6661 tttgacaaga aatatttgct agctgatagt gaccttaggc gacttttgaa cgcgcaataa
     6721 tggtttctga cgtatgtgct tagctcatta aactccagaa acccgcggct gagtggctcc
     6781 ttcaacgttg cggttctgtc agttccaaac gtaaaacggc ttgtcccgcg tcatcggcgg
     6841 gggtcataac gtgactccct taattctccg ctcatgatct tgatcccctg cgccatcaga
     6901 tccttggcgg caagaaagcc atccagttta ctttgcaggg cttcccaacc ttaccagagg
     6961 gcgccccagc tggcaattcc ggttcgcttg ctgtccataa aaccgcccag tctagctatc
     7021 gccatgtaag cccactgcaa gctacctgct ttctctttgc gcttgcgttt tcccttgtcc
     7081 agatagccca gtagctgaca ttcatccggg gtcagcaccg tttctgcgga ctggctttct
     7141 acgtgttccg cttcctttag cagcccttgc gccctgagtg cttgcggcag cgtgaagctg
     7201 atccgtcgag attaatagaa ataaattcag cctaattcgg cgttaattca gtacattaaa
     7261 aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc acaatatatc
     7321 ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc accactcgat
     7381 acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag gcggcagact
     7441 ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg tttgaaacac
     7501 ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg
     7561 atcaattcgg gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa
     7621 gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg
     7681 cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg
     7741 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
     7801 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatgg gggaagcggt
     7861 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
     7921 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca
     7981 cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
     8041 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
     8101 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg
     8161 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc
     8221 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
     8281 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
     8341 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
     8401 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
     8461 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
     8521 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca
     8581 gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg
     8641 tctagctaga aattcgttca agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta
     8701 gatgcactaa gcacataatt gctcacagcc aaactatcag gtcaagtctg cttttattat
     8761 ttttaagcgt gcataataag ccctacacaa attgggagat atatcatgca tgaccaaaat
     8821 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     8881 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     8941 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     9001 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     9061 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     9121 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     9181 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     9241 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     9301 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     9361 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     9421 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     9481 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     9541 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     9601 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct
     9661 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
     9721 cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt
     9781 gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     9841 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     9901 cagaggtttt caccgtcatc accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg
     9961 gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt agattgcctg gccgtaggcc
    10021 agccattttt gagcggccag cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga
    10081 gcgcagcgac cgaagggtag gcgctttttg cagctcttcg gctgtgcgct ggccagacag
    10141 ttatgcacag gccaggcggg ttttaagagt tttaataagt tttaaagagt tttaggcgga
    10201 aaaatcgcct tttttctctt ttatatcagt cacttacatg tgtgaccggt tcccaatgta
    10261 cggctttggg ttcccaatgt acgggttccg gttcccaatg tacggctttg ggttcccaat
    10321 gtacgtgcta tccacaggaa agagaccttt tcgacctttt tcccctgcta gggcaatttg
    10381 ccctagcatc tgctccgtac attaggaacc ggcggatgct tcgccctcga tcaggttgcg
    10441 gtagcgcatg actaggatcg ggccagcctg ccccgcctcc tccttcaaat cgtactccgg
    10501 caggtcattt gacccgatca gcttgcgcac ggtgaaacag aacttcttga actctccggc
    10561 gctgccactg cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc
    10621 gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa agtaatcggg
    10681 gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca tccaatcagc
    10741 tagctcgatc tcgatgtact ccggccgccc ggtttcgctc tttacgatct tgtagcggct
    10801 aatcaaggct tcaccctcgg ataccgtcac caggcggccg ttcttggcct tcttcgtacg
    10861 ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt cgtctttctg
    10921 ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg gacggaacac
    10981 gcggccgggc ttgtctccct tcccttcccg gtatcggttc atggattcgg ttagatggga
    11041 aaccgccatc agtaccaggt cgtaatccca cacactggcc atgccggccg gccctgcgga
    11101 aacctctacg tgcccgtctg gaagctcgta gcggatcacc tcgccagctc gtcggtcacg
    11161 cttcgacaga cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg tgcccacgtc
    11221 atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct tcctaatcga
    11281 cggcgcaccg gctgccggcg gttgccggga ttctttgcgg attcgatcag cggccgcttg
    11341 ccacgattca ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg cctgcgcggc
    11401 cttcaacttc tccaccaggt catcacccag cgccgcgccg atttgtaccg ggccggatgg
    11461 tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc cagtgccatt gcagggccgg
    11521 cagacaaccc agccgcttac gcctggccaa ccgcccgttc ctccacacat ggggcattcc
    11581 acggcgtcgg tgcctggttg ttcttgattt tccatgccgc ctcctttagc cgctaaaatt
    11641 catctactca tttattcatt tgctcattta ctctggtagc tgcgcgatgt attcagatag
    11701 cagctcggta atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc
    11761 cgccggcaac tgaaagttga cccgcttcat ggctggcgtg tctgccaggc tggccaacgt
    11821 tgcagccttg ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg tgcttttgct
    11881 cattttctct ttacctcatt aactcaaatg agttttgatt taatttcagc ggccagcgcc
    11941 tggacctcgc gggcagcgtc gccctcgggt tctgattcaa gaacggttgt gccggcggcg
    12001 gcagtgcctg ggtagctcac gcgctgcgtg atacgggact caagaatggg cagctcgtac
    12061 ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg
    12121 acaaaggccg cttgtagcct tccatccgtg acctcaatgc gctgcttaac cagctccacc
    12181 aggtcggcgg tggcccatat gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg
    12241 gctgccttga tcgcggacac agccaagtcc gccgcctggg gcgctccgtc gatcactacg
    12301 aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca
    12361 acggttagcg gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg
    12421 gaatcgacta acagaacatc ggccccggcg agttgcaggg cgcgggctag atgggttgcg
    12481 atggtcgtct tgcctgaccc gcctttctgg ttaagtacag cgataacctt catgcgttcc
    12541 ccttgcgtat ttgtttattt actcatcgca tcatatacgc agcgaccgca tgacgcaagc
    12601 tgttttactc aaatacacat caccttttta gacggcggcg ctcggtttct tcagcggcca
    12661 agctggccgg ccaggccgcc agcttggcat cagacaaacc ggccaggatt tcatgcagcc
    12721 gcacggttga gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc atctccgcct
    12781 cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg
    12841 ttcctcttgg cgttcattct cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc
    12901 acggaaggca ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc
    12961 gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc gcctctttca cggtgcggcc
    13021 ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg
    13081 gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat
    13141 tagggaacgc tcgaactcgg caatgccggc gaacacggtc aacaccatgc ggccggccgg
    13201 cgtggtggtg tcggcccacg gctctgccag gctacgcagg cccgcgccgg cctcctggat
    13261 gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac
    13321 tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg
    13381 cctggtgccg gtgatcttct cggaaaacag cttggtgcag ccggccgcgt gcagttcggc
    13441 ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc
    13501 ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat
    13561 gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt
    13621 aacttaggac ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac gtcagaagcc
    13681 gactgcacta tagcagcgga ggggttggat caaagtactt tgatcccgag gggaaccctg
    13741 tggttggcat gcacatacaa atggacgaac ggataaacct
//
