LOCUS       Exported               13784 bp ds-DNA     circular SYN 06-APR-2022
DEFINITION  Gateway cloning compatible binary vector for N-terminal fusion with 
            sGFP (CaMV35S promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    pGW-GFP-Gene-TurboID
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13784)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 13784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Sep 19, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13784
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1178..1888
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELY"
     primer_bind     complement(1220..1241)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1481..1500)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1828..1849
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1892..2016
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2053..2083
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2137..2796
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2203..2222)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3138..3443
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3322..3341
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3484..3608)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3628..3654
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3658..3684
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3688..3714
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3721..4677
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     primer_bind     complement(4276..4292)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     terminator      4705..4957
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4991..5007)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4991..5007)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5004..5026)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5015..5031
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5039..5069)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5084..5105
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      5141..5393
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5544..6569)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(6621..6800)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    7306..7330
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7851..8642
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8888..9476
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9377..9396
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9630..9647
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9662..9802
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9728..9747)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9888..9910
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10146..10340
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10406..11479)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11908..12537)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aggatccatg agcaagggcg aggagctgtt
     1201 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtgaacggcc acaagttcag
     1261 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     1321 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttca cctacggcgt
     1381 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     1441 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     1501 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     1561 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     1621 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     1681 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     1741 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     1801 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     1861 gatcactcac ggcatggacg agctgtacat cacaagtttg tacaaaaaag ctgaacgaga
     1921 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac
     1981 ataatactgt aaaacacaac atatccagtc actatggcgg cccctcgagg ggccgcatta
     2041 ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag
     2101 gatccggcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
     2161 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
     2221 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
     2281 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
     2341 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
     2401 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
     2461 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
     2521 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
     2581 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
     2641 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
     2701 attcaggttc atcatgccgt ctgtgatggc ttccatgtcg gcagaatgct taatgaatta
     2761 caacagtact gcgatgagtg gcagggcggg gcgtaaacgc gtggatccgg cttactaaaa
     2821 gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat
     2881 atgtataccc gaagtatgtc aaaaagaggt gtgctatgaa gcagcgtatt acagtgacag
     2941 ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt
     3001 aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa
     3061 tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga
     3121 gaacagggac tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc
     3181 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc
     3241 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc
     3301 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg
     3361 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
     3421 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc
     3481 gaccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
     3541 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac
     3601 aaagtggttc gatctagagg atccatgtac ccgtatgatg ttccggatta cgctggctat
     3661 ccctacgacg tgcccgacta tgccgggtac ccctatgacg tcccagacta cgcagctagc
     3721 aaagacaata ctgtgcctct gaagctgatc gctctcctgg ctaatggcga gttccatagt
     3781 ggcgaacagc tgggagaaac cctgggcatg tccagggccg ctatcaacaa gcacattcag
     3841 actctgcgcg actggggcgt ggacgtgttc accgtgcccg gaaagggcta ctctctgccc
     3901 gagcctatcc cgctgctgaa cgctaaacag attctgggac agctggacgg cgggagcgtg
     3961 gcagtcctgc ctgtggtaga ctccaccaat cagtacctgc tggatcgaat cggcgagctg
     4021 aagagtgggg atgcttgcat tgcagaatat cagcaggcag ggagaggaag cagagggagg
     4081 aaatggttct ctccttttgg agctaacctg tacctgagta tgttttggcg cctgaagcgg
     4141 ggaccagcag caatcggcct gggcccggtc atcggaattg tcatggcaga agcgctgcga
     4201 aagctgggag cagacaaggt gcgagtcaaa tggcccaatg acctgtatct gcaggataga
     4261 aagctggcag gcatcctggt ggagctggcc ggaataacag gcgatgctgc acagatcgtc
     4321 attggcgccg ggattaacgt ggctatgagg cgcgtggagg aaagcgtggt caatcagggc
     4381 tggatcacac tgcaggaagc agggattaac ctggacagga atactctggc cgctacgctg
     4441 atccgagagc tgcgggcagc cctggaactg ttcgagcagg aaggcctggc tccatatctg
     4501 ccacggtggg agaagctgga taacttcatc aatagacccg tgaagctgat cattggggac
     4561 aaagagattt tcgggattag ccgggggatt gataaacagg gagccctgct gctggaacag
     4621 gacggagtta tcaaaccctg gatgggcgga gaaatcagtc tgcggtctgc cgaaaagtag
     4681 agcggcccga gctcgaattt ccccgatcgt tcaaacattt ggcaataaag tttcttaaga
     4741 ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag
     4801 catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga
     4861 gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat
     4921 aaattatcgc gcgcggtgtc atctatgtta ctagatcggg aattggttcc ggaaccaatt
     4981 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     5041 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     5101 cattaggctg aattaggcgc gcctatttct attaattccc gatctagtaa catagatgac
     5161 accgcgcgcg ataatttatc ctagtttgcg cgctatattt tgttttctat cgcgtattaa
     5221 atgtataatt gcgggactct aatcataaaa acccatctca taaataacgt catgcattac
     5281 atgttaatta ttacatgctt aacgtaattc aacagaaatt atatgataat catcgcaaga
     5341 ccggcaacag gattcaatct taagaaactt tattgccaaa tgtttgaacg atcggggaaa
     5401 ttcgggggat cgatcccgcg aactgtggac gagaactgtg ccaccaagcg taaggccgtt
     5461 ctctcgcatt tgccttgcta ggctcgcgcg agttgctggc tgaggcgttc tcgaaatcag
     5521 ctcttgttcg gtcggcatct actctattcc tttgccctcg gacgagtgct ggggcgtcgg
     5581 tttccactat cggcgagtac ttctacacag ccatcggtcc agacggccgc gcttctgcgg
     5641 gcgatttgtg tacgcccgac agtcccggct ccggatcgga cgattgcgtc gcatcgaccc
     5701 tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc tctgatagag ttggtcaaga
     5761 ccaatgcgga gcatatacgc ccggagccgc ggcgatcctg caagctccgg atgcctccgc
     5821 tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg gcctccagaa gaagatgttg
     5881 gcgacctcgt attgggaatc cccgaacatc gcctcgctcc agtcaatgac cgctgttatg
     5941 cggccattgt ccgtcaggac attgttggag ccgaaatccg cgtgcacgag gtgccggact
     6001 tcggggcagt cctcggccca aagcatcagc tcatcgagag cctgcgcgac ggacgcactg
     6061 acggtgtcgt ccatcacagt ttgccagtga tacacatggg gatcagcaat cgcgcatatg
     6121 aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc
     6181 gtctggctaa gatcggccgc agcgatcgca tccatggcct ccgcgaccgg ctgcagaaca
     6241 gcgggcagtt cggtttcagg caggtcttgc aacgtgacac cctgtgcacg gcgggagatg
     6301 caataggtca ggctctcgct gaatgcccca atgtcaagca cttccggaat cgggagcgcg
     6361 gccgatgcaa agtgccgata aacataacga tctttgtaga aaccatcggc gcagctattt
     6421 acccgcagga catatccacg ccctcctaca tcgaagctga aagcacgaga ttcttcgccc
     6481 tccgagagct gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa cttctcgaca
     6541 gacgtcgcgg tgagttcagg ctttttcata tcttattgcc ccccgggatc agatctggat
     6601 tgagagtgaa tatgagactc taattggata ccgaggggaa tttatggaac gtcagtggag
     6661 catttttgac aagaaatatt tgctagctga tagtgacctt aggcgacttt tgaacgcgca
     6721 ataatggttt ctgacgtatg tgcttagctc attaaactcc agaaacccgc ggctgagtgg
     6781 ctccttcaac gttgcggttc tgtcagttcc aaacgtaaaa cggcttgtcc cgcgtcatcg
     6841 gcgggggtca taacgtgact cccttaattc tccgctcatg atcttgatcc cctgcgccat
     6901 cagatccttg gcggcaagaa agccatccag tttactttgc agggcttccc aaccttacca
     6961 gagggcgccc cagctggcaa ttccggttcg cttgctgtcc ataaaaccgc ccagtctagc
     7021 tatcgccatg taagcccact gcaagctacc tgctttctct ttgcgcttgc gttttccctt
     7081 gtccagatag cccagtagct gacattcatc cggggtcagc accgtttctg cggactggct
     7141 ttctacgtgt tccgcttcct ttagcagccc ttgcgccctg agtgcttgcg gcagcgtgaa
     7201 gctgatccgt cgagattaat agaaataaat tcagcctaat tcggcgttaa ttcagtacat
     7261 taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata
     7321 tatcctgcca ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact
     7381 cgatacaggc agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca
     7441 gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa
     7501 acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc
     7561 tgtgatcaat tcgggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
     7621 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
     7681 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
     7741 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
     7801 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgggggaag
     7861 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
     7921 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
     7981 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
     8041 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
     8101 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
     8161 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
     8221 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
     8281 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
     8341 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
     8401 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     8461 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     8521 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     8581 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     8641 aatgtctagc tagaaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc
     8701 gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta
     8761 ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca tgcatgacca
     8821 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     8881 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     8941 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     9001 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     9061 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     9121 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     9181 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     9241 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     9301 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     9361 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     9421 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     9481 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     9541 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     9601 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     9661 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
     9721 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct
     9781 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg
     9841 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     9901 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagggtgcct tgatgtgggc
     9961 gccggcggtc gagtggcgac ggcgcggctt gtccgcgccc tggtagattg cctggccgta
    10021 ggccagccat ttttgagcgg ccagcggccg cgataggccg acgcgaagcg gcggggcgta
    10081 gggagcgcag cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg cgctggccag
    10141 acagttatgc acaggccagg cgggttttaa gagttttaat aagttttaaa gagttttagg
    10201 cggaaaaatc gccttttttc tcttttatat cagtcactta catgtgtgac cggttcccaa
    10261 tgtacggctt tgggttccca atgtacgggt tccggttccc aatgtacggc tttgggttcc
    10321 caatgtacgt gctatccaca ggaaagagac cttttcgacc tttttcccct gctagggcaa
    10381 tttgccctag catctgctcc gtacattagg aaccggcgga tgcttcgccc tcgatcaggt
    10441 tgcggtagcg catgactagg atcgggccag cctgccccgc ctcctccttc aaatcgtact
    10501 ccggcaggtc atttgacccg atcagcttgc gcacggtgaa acagaacttc ttgaactctc
    10561 cggcgctgcc actgcgttcg tagatcgtct tgaacaacca tctggcttct gccttgcctg
    10621 cggcgcggcg tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc aaaaagtaat
    10681 cggggtgaac cgtcagcacg tccgggttct tgccttctgt gatctcgcgg tacatccaat
    10741 cagctagctc gatctcgatg tactccggcc gcccggtttc gctctttacg atcttgtagc
    10801 ggctaatcaa ggcttcaccc tcggataccg tcaccaggcg gccgttcttg gccttcttcg
    10861 tacgctgcat ggcaacgtgc gtggtgttta accgaatgca ggtttctacc aggtcgtctt
    10921 tctgctttcc gccatcggct cgccggcaga acttgagtac gtccgcaacg tgtggacgga
    10981 acacgcggcc gggcttgtct cccttccctt cccggtatcg gttcatggat tcggttagat
    11041 gggaaaccgc catcagtacc aggtcgtaat cccacacact ggccatgccg gccggccctg
    11101 cggaaacctc tacgtgcccg tctggaagct cgtagcggat cacctcgcca gctcgtcggt
    11161 cacgcttcga cagacggaaa acggccacgt ccatgatgct gcgactatcg cgggtgccca
    11221 cgtcatagag catcggaacg aaaaaatctg gttgctcgtc gcccttgggc ggcttcctaa
    11281 tcgacggcgc accggctgcc ggcggttgcc gggattcttt gcggattcga tcagcggccg
    11341 cttgccacga ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg gcggcctgcg
    11401 cggccttcaa cttctccacc aggtcatcac ccagcgccgc gccgatttgt accgggccgg
    11461 atggtttgcg accgctcacg ccgattcctc gggcttgggg gttccagtgc cattgcaggg
    11521 ccggcagaca acccagccgc ttacgcctgg ccaaccgccc gttcctccac acatggggca
    11581 ttccacggcg tcggtgcctg gttgttcttg attttccatg ccgcctcctt tagccgctaa
    11641 aattcatcta ctcatttatt catttgctca tttactctgg tagctgcgcg atgtattcag
    11701 atagcagctc ggtaatggtc ttgccttggc gtaccgcgta catcttcagc ttggtgtgat
    11761 cctccgccgg caactgaaag ttgacccgct tcatggctgg cgtgtctgcc aggctggcca
    11821 acgttgcagc cttgctgctg cgtgcgctcg gacggccggc acttagcgtg tttgtgcttt
    11881 tgctcatttt ctctttacct cattaactca aatgagtttt gatttaattt cagcggccag
    11941 cgcctggacc tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg ttgtgccggc
    12001 ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa tgggcagctc
    12061 gtacccggcc agcgcctcgg caacctcacc gccgatgcgc gtgcctttga tcgcccgcga
    12121 cacgacaaag gccgcttgta gccttccatc cgtgacctca atgcgctgct taaccagctc
    12181 caccaggtcg gcggtggccc atatgtcgta agggcttggc tgcaccggaa tcagcacgaa
    12241 gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc cgtcgatcac
    12301 tacgaagtcg cgccggccga tggccttcac gtcgcggtca atcgtcgggc ggtcgatgcc
    12361 gacaacggtt agcggttgat cttcccgcac ggccgcccaa tcgcgggcac tgccctgggg
    12421 atcggaatcg actaacagaa catcggcccc ggcgagttgc agggcgcggg ctagatgggt
    12481 tgcgatggtc gtcttgcctg acccgccttt ctggttaagt acagcgataa ccttcatgcg
    12541 ttccccttgc gtatttgttt atttactcat cgcatcatat acgcagcgac cgcatgacgc
    12601 aagctgtttt actcaaatac acatcacctt tttagacggc ggcgctcggt ttcttcagcg
    12661 gccaagctgg ccggccaggc cgccagcttg gcatcagaca aaccggccag gatttcatgc
    12721 agccgcacgg ttgagacgtg cgcgggcggc tcgaacacgt acccggccgc gatcatctcc
    12781 gcctcgatct cttcggtaat gaaaaacggt tcgtcctggc cgtcctggtg cggtttcatg
    12841 cttgttcctc ttggcgttca ttctcggcgg ccgccagggc gtcggcctcg gtcaatgcgt
    12901 cctcacggaa ggcaccgcgc cgcctggcct cggtgggcgt cacttcctcg ctgcgctcaa
    12961 gtgcgcggta cagggtcgag cgatgcacgc caagcagtgc agccgcctct ttcacggtgc
    13021 ggccttcctg gtcgatcagc tcgcgggcgt gcgcgatctg tgccggggtg agggtagggc
    13081 gggggccaaa cttcacgcct cgggccttgg cggcctcgcg cccgctccgg gtgcggtcga
    13141 tgattaggga acgctcgaac tcggcaatgc cggcgaacac ggtcaacacc atgcggccgg
    13201 ccggcgtggt ggtgtcggcc cacggctctg ccaggctacg caggcccgcg ccggcctcct
    13261 ggatgcgctc ggcaatgtcc agtaggtcgc gggtgctgcg ggccaggcgg tctagcctgg
    13321 tcactgtcac aacgtcgcca gggcgtaggt ggtcaagcat cctggccagc tccgggcggt
    13381 cgcgcctggt gccggtgatc ttctcggaaa acagcttggt gcagccggcc gcgtgcagtt
    13441 cggcccgttg gttggtcaag tcctggtcgt cggtgctgac gcgggcatag cccagcaggc
    13501 cagcggcggc gctcttgttc atggcgtaat gtctccggtt ctagtcgcaa gtattctact
    13561 ttatgcgact aaaacacgcg acaagaaaac gccaggaaaa gggcagggcg gcagcctgtc
    13621 gcgtaactta ggacttgtgc gacatgtcgt tttcagaaga cggctgcact gaacgtcaga
    13681 agccgactgc actatagcag cggaggggtt ggatcaaagt actttgatcc cgaggggaac
    13741 cctgtggttg gcatgcacat acaaatggac gaacggataa acct
//
