LOCUS       Exported               13790 bp ds-DNA     circular SYN 06-APR-2022
DEFINITION  Gateway cloning compatible binary vector for C-terminal fusion with 
            sGFP (CaMV35S promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    pGW-TurboID-Gene-GFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13790)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 13790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Sep 19, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13790
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1181..1207
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1211..1237
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1241..1267
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1274..2230
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    2234..2358
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2395..2425
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2479..3138
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2545..2564)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3480..3785
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3664..3683
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3826..3950)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3967..4686
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(4273..4292)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4620..4641
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      4711..4963
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4997..5013)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4997..5013)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5010..5032)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5021..5037
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5045..5075)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5090..5111
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      5147..5399
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5550..6575)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(6627..6806)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    7312..7336
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7857..8648
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8894..9482
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9383..9402
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9636..9653
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9668..9808
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9734..9753)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9894..9916
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10152..10346
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10412..11485)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11914..12543)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aggatccatg tacccgtatg atgttccgga
     1201 ttacgctggc tatccctacg acgtgcccga ctatgccggg tacccctatg acgtcccaga
     1261 ctacgcagct agcaaagaca atactgtgcc tctgaagctg atcgctctcc tggctaatgg
     1321 cgagttccat agtggcgaac agctgggaga aaccctgggc atgtccaggg ccgctatcaa
     1381 caagcacatt cagactctgc gcgactgggg cgtggacgtg ttcaccgtgc ccggaaaggg
     1441 ctactctctg cccgagccta tcccgctgct gaacgctaaa cagattctgg gacagctgga
     1501 cggcgggagc gtggcagtcc tgcctgtggt agactccacc aatcagtacc tgctggatcg
     1561 aatcggcgag ctgaagagtg gggatgcttg cattgcagaa tatcagcagg cagggagagg
     1621 aagcagaggg aggaaatggt tctctccttt tggagctaac ctgtacctga gtatgttttg
     1681 gcgcctgaag cggggaccag cagcaatcgg cctgggcccg gtcatcggaa ttgtcatggc
     1741 agaagcgctg cgaaagctgg gagcagacaa ggtgcgagtc aaatggccca atgacctgta
     1801 tctgcaggat agaaagctgg caggcatcct ggtggagctg gccggaataa caggcgatgc
     1861 tgcacagatc gtcattggcg ccgggattaa cgtggctatg aggcgcgtgg aggaaagcgt
     1921 ggtcaatcag ggctggatca cactgcagga agcagggatt aacctggaca ggaatactct
     1981 ggccgctacg ctgatccgag agctgcgggc agccctggaa ctgttcgagc aggaaggcct
     2041 ggctccatat ctgccacggt gggagaagct ggataacttc atcaatagac ccgtgaagct
     2101 gatcattggg gacaaagaga ttttcgggat tagccggggg attgataaac agggagccct
     2161 gctgctggaa caggacggag ttatcaaacc ctggatgggc ggagaaatca gtctgcggtc
     2221 tgccgaaaag atcacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa
     2281 tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca
     2341 acatatccag tcactatggc ggcccctcga ggggccgcat taggcacccc aggctttaca
     2401 ctttatgctt ccggctcgta taatgtgtgg attttgagtt aggatccgtc gagattttca
     2461 ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc
     2521 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac
     2581 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
     2641 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt
     2701 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt
     2761 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg
     2821 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc
     2881 cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc
     2941 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc
     3001 aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc
     3061 gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag
     3121 tggcagggcg gggcgtaatc tagaggatcc ggcttactaa aagccagata acagtatgcg
     3181 tatttgcgcg ctgatttttg cggtataaga atatatactg atatgtatac ccgaagtatg
     3241 tcaaaaagag gtatgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc
     3301 agttgctcaa ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa
     3361 tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga
     3421 ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac gagaacaggg gctggtgaaa
     3481 tgcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac
     3541 agagtgatat tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcacgtc
     3601 tgctgtcaga taaagtcccc cgtgaacttt acccggtggt gcatatcggg gatgaaagct
     3661 ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg gaagaagtgg
     3721 ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa
     3781 tataaatgtc aggctccctt atacacagcc agtctgcagg tcgaccatag tgactggata
     3841 tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg
     3901 atatttatat cattttacgt ttctcgttca gctttcttgt acaaagtggt tcgatctaga
     3961 ggatccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     4021 ctggacggcg acgtgaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     4081 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     4141 cccaccctcg tgaccacctt cacctacggc gtgcagtgct tcagccgcta ccccgaccac
     4201 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     4261 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     4321 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     4381 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     4441 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     4501 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     4561 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     4621 atggtcctgc tggagttcgt gaccgccgcc gggatcactc acggcatgga cgagctgtac
     4681 aagtaaagcg gcccgagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc
     4741 ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac
     4801 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg
     4861 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac
     4921 taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt ggttccggaa
     4981 ccaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     5041 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     5101 aactcacatt aggctgaatt aggcgcgcct atttctatta attcccgatc tagtaacata
     5161 gatgacaccg cgcgcgataa tttatcctag tttgcgcgct atattttgtt ttctatcgcg
     5221 tattaaatgt ataattgcgg gactctaatc ataaaaaccc atctcataaa taacgtcatg
     5281 cattacatgt taattattac atgcttaacg taattcaaca gaaattatat gataatcatc
     5341 gcaagaccgg caacaggatt caatcttaag aaactttatt gccaaatgtt tgaacgatcg
     5401 gggaaattcg ggggatcgat cccgcgaact gtggacgaga actgtgccac caagcgtaag
     5461 gccgttctct cgcatttgcc ttgctaggct cgcgcgagtt gctggctgag gcgttctcga
     5521 aatcagctct tgttcggtcg gcatctactc tattcctttg ccctcggacg agtgctgggg
     5581 cgtcggtttc cactatcggc gagtacttct acacagccat cggtccagac ggccgcgctt
     5641 ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg atcggacgat tgcgtcgcat
     5701 cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg
     5761 tcaagaccaa tgcggagcat atacgcccgg agccgcggcg atcctgcaag ctccggatgc
     5821 ctccgctcga agtagcgcgt ctgctgctcc atacaagcca accacggcct ccagaagaag
     5881 atgttggcga cctcgtattg ggaatccccg aacatcgcct cgctccagtc aatgaccgct
     5941 gttatgcggc cattgtccgt caggacattg ttggagccga aatccgcgtg cacgaggtgc
     6001 cggacttcgg ggcagtcctc ggcccaaagc atcagctcat cgagagcctg cgcgacggac
     6061 gcactgacgg tgtcgtccat cacagtttgc cagtgataca catggggatc agcaatcgcg
     6121 catatgaaat cacgccatgt agtgtattga ccgattcctt gcggtccgaa tgggccgaac
     6181 ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca tggcctccgc gaccggctgc
     6241 agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg tgcacggcgg
     6301 gagatgcaat aggtcaggct ctcgctgaat gccccaatgt caagcacttc cggaatcggg
     6361 agcgcggccg atgcaaagtg ccgataaaca taacgatctt tgtagaaacc atcggcgcag
     6421 ctatttaccc gcaggacata tccacgccct cctacatcga agctgaaagc acgagattct
     6481 tcgccctccg agagctgcat caggtcggag acgctgtcga acttttcgat cagaaacttc
     6541 tcgacagacg tcgcggtgag ttcaggcttt ttcatatctt attgcccccc gggatcagat
     6601 ctggattgag agtgaatatg agactctaat tggataccga ggggaattta tggaacgtca
     6661 gtggagcatt tttgacaaga aatatttgct agctgatagt gaccttaggc gacttttgaa
     6721 cgcgcaataa tggtttctga cgtatgtgct tagctcatta aactccagaa acccgcggct
     6781 gagtggctcc ttcaacgttg cggttctgtc agttccaaac gtaaaacggc ttgtcccgcg
     6841 tcatcggcgg gggtcataac gtgactccct taattctccg ctcatgatct tgatcccctg
     6901 cgccatcaga tccttggcgg caagaaagcc atccagttta ctttgcaggg cttcccaacc
     6961 ttaccagagg gcgccccagc tggcaattcc ggttcgcttg ctgtccataa aaccgcccag
     7021 tctagctatc gccatgtaag cccactgcaa gctacctgct ttctctttgc gcttgcgttt
     7081 tcccttgtcc agatagccca gtagctgaca ttcatccggg gtcagcaccg tttctgcgga
     7141 ctggctttct acgtgttccg cttcctttag cagcccttgc gccctgagtg cttgcggcag
     7201 cgtgaagctg atccgtcgag attaatagaa ataaattcag cctaattcgg cgttaattca
     7261 gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc
     7321 acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc
     7381 accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag
     7441 gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg
     7501 tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt
     7561 gctgcctgtg atcaattcgg gcacgaaccc agtggacata agcctgttcg gttcgtaagc
     7621 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
     7681 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt
     7741 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
     7801 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatgg
     7861 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
     7921 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
     7981 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
     8041 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga
     8101 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
     8161 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct
     8221 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
     8281 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
     8341 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
     8401 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
     8461 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
     8521 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc
     8581 gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg
     8641 gcaaataatg tctagctaga aattcgttca agccgacgcc gcttcgcggc gcggcttaac
     8701 tcaagcgtta gatgcactaa gcacataatt gctcacagcc aaactatcag gtcaagtctg
     8761 cttttattat ttttaagcgt gcataataag ccctacacaa attgggagat atatcatgca
     8821 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     8881 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     8941 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     9001 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     9061 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     9121 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     9181 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     9241 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     9301 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     9361 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     9421 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     9481 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     9541 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     9601 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     9661 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
     9721 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct
     9781 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc
     9841 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     9901 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagg gtgccttgat
     9961 gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt agattgcctg
    10021 gccgtaggcc agccattttt gagcggccag cggccgcgat aggccgacgc gaagcggcgg
    10081 ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg cagctcttcg gctgtgcgct
    10141 ggccagacag ttatgcacag gccaggcggg ttttaagagt tttaataagt tttaaagagt
    10201 tttaggcgga aaaatcgcct tttttctctt ttatatcagt cacttacatg tgtgaccggt
    10261 tcccaatgta cggctttggg ttcccaatgt acgggttccg gttcccaatg tacggctttg
    10321 ggttcccaat gtacgtgcta tccacaggaa agagaccttt tcgacctttt tcccctgcta
    10381 gggcaatttg ccctagcatc tgctccgtac attaggaacc ggcggatgct tcgccctcga
    10441 tcaggttgcg gtagcgcatg actaggatcg ggccagcctg ccccgcctcc tccttcaaat
    10501 cgtactccgg caggtcattt gacccgatca gcttgcgcac ggtgaaacag aacttcttga
    10561 actctccggc gctgccactg cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct
    10621 tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa
    10681 agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca
    10741 tccaatcagc tagctcgatc tcgatgtact ccggccgccc ggtttcgctc tttacgatct
    10801 tgtagcggct aatcaaggct tcaccctcgg ataccgtcac caggcggccg ttcttggcct
    10861 tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt
    10921 cgtctttctg ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg
    10981 gacggaacac gcggccgggc ttgtctccct tcccttcccg gtatcggttc atggattcgg
    11041 ttagatggga aaccgccatc agtaccaggt cgtaatccca cacactggcc atgccggccg
    11101 gccctgcgga aacctctacg tgcccgtctg gaagctcgta gcggatcacc tcgccagctc
    11161 gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg
    11221 tgcccacgtc atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct
    11281 tcctaatcga cggcgcaccg gctgccggcg gttgccggga ttctttgcgg attcgatcag
    11341 cggccgcttg ccacgattca ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg
    11401 cctgcgcggc cttcaacttc tccaccaggt catcacccag cgccgcgccg atttgtaccg
    11461 ggccggatgg tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc cagtgccatt
    11521 gcagggccgg cagacaaccc agccgcttac gcctggccaa ccgcccgttc ctccacacat
    11581 ggggcattcc acggcgtcgg tgcctggttg ttcttgattt tccatgccgc ctcctttagc
    11641 cgctaaaatt catctactca tttattcatt tgctcattta ctctggtagc tgcgcgatgt
    11701 attcagatag cagctcggta atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg
    11761 tgtgatcctc cgccggcaac tgaaagttga cccgcttcat ggctggcgtg tctgccaggc
    11821 tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg
    11881 tgcttttgct cattttctct ttacctcatt aactcaaatg agttttgatt taatttcagc
    11941 ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt tctgattcaa gaacggttgt
    12001 gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg atacgggact caagaatggg
    12061 cagctcgtac ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc
    12121 ccgcgacacg acaaaggccg cttgtagcct tccatccgtg acctcaatgc gctgcttaac
    12181 cagctccacc aggtcggcgg tggcccatat gtcgtaaggg cttggctgca ccggaatcag
    12241 cacgaagtcg gctgccttga tcgcggacac agccaagtcc gccgcctggg gcgctccgtc
    12301 gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc
    12361 gatgccgaca acggttagcg gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc
    12421 ctggggatcg gaatcgacta acagaacatc ggccccggcg agttgcaggg cgcgggctag
    12481 atgggttgcg atggtcgtct tgcctgaccc gcctttctgg ttaagtacag cgataacctt
    12541 catgcgttcc ccttgcgtat ttgtttattt actcatcgca tcatatacgc agcgaccgca
    12601 tgacgcaagc tgttttactc aaatacacat caccttttta gacggcggcg ctcggtttct
    12661 tcagcggcca agctggccgg ccaggccgcc agcttggcat cagacaaacc ggccaggatt
    12721 tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc
    12781 atctccgcct cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt
    12841 ttcatgcttg ttcctcttgg cgttcattct cggcggccgc cagggcgtcg gcctcggtca
    12901 atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc
    12961 gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc gcctctttca
    13021 cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg
    13081 tagggcgggg gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc
    13141 ggtcgatgat tagggaacgc tcgaactcgg caatgccggc gaacacggtc aacaccatgc
    13201 ggccggccgg cgtggtggtg tcggcccacg gctctgccag gctacgcagg cccgcgccgg
    13261 cctcctggat gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta
    13321 gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg gccagctccg
    13381 ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag cttggtgcag ccggccgcgt
    13441 gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca
    13501 gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat
    13561 tctactttat gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag
    13621 cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac
    13681 gtcagaagcc gactgcacta tagcagcgga ggggttggat caaagtactt tgatcccgag
    13741 gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
//
